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稻草秸秆厌氧发酵产沼气研究 总被引:3,自引:0,他引:3
[目的]对影响稻草秸秆厌氧生产生物沼气的重要因素进行分析,以提高产气率,最大限度地提高秸杆原料利用率。[方法]采用单因素法对南京工业大学沼气站生产沼气的接种量、总固体含量、碳氮比进行分析。[结果]沼气站厌氧发酵的最优条件为接种量34%,总固体含量6%,碳氮比25:1。经验证,最优配比下的总产气量比目前常用的发酵条件(接种量50%,总固体含量5.6%,碳氮比22:1)下的总产气量提高了27.60%。[结论]为进一步研究利用秸秆发酵产沼气提供了依据。 相似文献
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稻草秸秆厌氧发酵产沼气研究 总被引:2,自引:1,他引:2
[目的]对影响稻草秸秆厌氧生产生物沼气的重要因素进行分析,以提高产气率,最大限度地提高秸秆原料利用率。[方法]采用单因素法对南京工业大学沼气站生产沼气的接种量、总固体含量、碳氮比进行分析。[结果]沼气站厌氧发酵的最优条件为接种量34%,总固体含量6%,碳氮比25∶1。经验证,最优配比下的总产气量比目前常用的发酵条件(接种量50%,总固体含量5.6%,碳氮比22∶1)下的总产气量提高了27.60%。[结论]为进一步研究利用秸秆发酵产沼气提供了依据。 相似文献
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长绒毛栓菌(Trametes villosa DSM 9591)产漆酶的发酵条件优化 总被引:1,自引:0,他引:1
[目的]通过响应面法优化长绒毛栓菌发酵培养基,提高漆酶的产量。[方法]首先通过Plackett-Burman筛选出影响漆酶产量的3个主要因素;随后以葡萄糖浓度、二甲基苯胺浓度、摇床转速3因素自变量,采用Box-Behnken设计方法进行响应面试验,得到最佳发酵条件。[结果]回归分析得到最佳发酵条件方程,最佳的发酵条件为葡萄糖浓度12.37 g/L,二甲基苯胺浓度1.348 mmol/L,转速200r/min,此时漆酶的产量最高,经验证达4.1 U/ml,比初始条件提高了1.56倍。在10 L的发酵罐中,在1.0 VVM、350 r/min条件下培养时,漆酶产量最高可达13.6 U/ml。[结论]该研究得到了长绒毛栓菌产漆酶最佳的培养条件,为漆酶生产提供了更好的方法,为其今后的工业化生产奠定了基础。 相似文献
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[Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Navicula tenera was preliminarily investigated based on the consideration of regional characteristics; and a series of culture conditions including the nutrient source of nitrogen(N), phosphorus(P), iron(Fe), silicon(Si) and the salinity in medium for culturing Navicula tenera, were optimized by single factor test and orthogonal design. [Result] The optimized conditions for cultivating Navicula tenera using sewage are as follows: the water from Xiaoerlou Artificial Lake of Nanjing University of Technology as basic solvent; 360 mg/L urea; 150 mg/L N2HPO4·12H2O; 50 mg/L ferric citrate; 2 000 mg/L Na2SiO3·9H2O; 2.0 mol/L salinity. Navicula tenera was strongly adaptive to sewage and could well uptake the nutrient sources in the sewage. Under the optimized conditions, the culture cost decreased, and meanwhile the biomass of Navicula tenera reached 4.766 g/L which is 3.57 multiples over original medium and 1.9 multiples over optimized medium No. 1. [Conclusion] This study laid a foundation for the combination of culturing Navicula tenera in large scale and sewage treatment. 相似文献