Resistance of wheat to Mycosphaerella graminicola involves early and late peaks of gene expression     

Tika B. Adhikari  Boovaraghan Balaji  Jill Breeden  Stephen B. Goodwin   《Physiological and Molecular Plant Pathology》2007,71(1-3):55-68

Large-scale cDNA-AFLP profiling identified numerous genes with increased expression during the resistance response of wheat to the Septoria tritici blotch fungus, Mycosphaerella graminicola. To test whether these genes were associated with resistance responses, primers were designed for the 14 that were most strongly up-regulated, and their levels of expression were measured at 12 time points from 0 to 27 days after inoculation (DAI) in two resistant and two susceptible cultivars of wheat by real-time quantitative polymerase chain reaction. None of these genes was expressed constitutively in the resistant wheat cultivars. Instead, infection of wheat by M. graminicola induced changes in expression of each gene in both resistant and susceptible cultivars over time. The four genes chitinase, phenylalanine ammonia lyase, pathogenesis-related protein PR-1, and peroxidase were induced from about 10- to 60-fold at early stages (3 h–1 DAI) during the incompatible interactions but were not expressed at later time points. Nine other genes (ATPase, brassinosteroid-6-oxidase, peptidylprolyl isomerase, peroxidase 2, 40S ribosomal protein, ADP-glucose pyrophosphorylase, putative protease inhibitor, methionine sulfoxide reductase, and an RNase S-like protein precursor) had bimodal patterns with both early (1–3 DAI) and late (12–24 DAI) peaks of expression in at least one of the resistant cultivars, but low if any induction in the two susceptible cultivars. The remaining gene (a serine carboxypeptidase) had a trimodal pattern of expression in the resistant cultivar Tadinia. These results indicate that the resistance response of wheat to M. graminicola is not completed during the first 24 h after contact with the pathogen, as thought previously, but instead can extend into the period from 18 to 24 DAI when fungal growth increases dramatically in compatible interactions. Many of these genes have a possible function in signal transduction or possibly as regulatory elements. Expression of the PR-1 gene at 12 h after inoculation was much higher in resistant compared to susceptible recombinant-inbred lines (RILs) segregating for the Stb4 and Stb8 genes for resistance. Therefore, analysis of gene expression could provide a faster method for separating resistant from susceptible lines in research programs. Significant differential expression patterns of the defense-related genes between the resistant and susceptible wheat cultivars and RILs after inoculation with M. graminicola suggest that these genes may play a major role in the resistance mechanisms of wheat.  相似文献   

Rural household and forest: an evaluation of household’s dependency on community forest in Nepal     

Mohan?AdhikariEmail author  Shin?Nagata  Murali?Adhikari 《Journal of Forest Research》2004,9(1):33-44

The aim of this paper is to analyze the level of dependency of rural households on forests, based on the economic status of the households and the property regimes of forests. The analysis of household-level dependency suggests that large numbers of rural households are benefiting regularly from their community forests. Out of three forest property regimes examined, community forests were found to be the most prominent sources for supplying forest products to local households. Analysis of the degree of forest-products dependency and the economic status of households shows that the low economic status households rely more on the community forest than the economically better-off households. In addition, a ² analysis indicates that the economic status of households affects the degree of forest-product collection activities. The results show three types of property regime with respect to forest management in Chitwan. Each regime holds its own ownership rights, which control the harvesting of forest products and provides incentives to the local households.  相似文献   

Variability of soil carbon in row crop watersheds with agroforestry buffers     

Ranjith P. Udawatta  Pradip Adhikari  G. M. M. M. Anoma Senaviratne  Harold E. Garrett 《Agroforestry Systems》2015,89(1):37-47

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Inheritance of the stem rust resistance phenotype Pg-a in oats     

K.N. Adhikari  R.A. McIntosh  J.D. Oates 《Euphytica》1999,105(2):143-154

Forty stem rust resistant oat cultivars and lines were selected from Australian oat breeding programs, Quaker Oat Nurseries and a Brazilian oat collection. Most, including the Pg-a reference stock Omega, were crossed with a susceptible line in order to study the inheritance of resistance. Omega was crossed with 15 resistant selections and a further 15 intercrosses were made among resistant selections. All selected lines possessed Pg-a which was inherited as a pair of recessive complementary genes. All crosses among resistant lines failed to segregate. In the majority of resistant/susceptible crosses, the F2 ratio was 1 resistant: 2 intermediate: 13 susceptible, whereas in others it was 1 resistant: 15 susceptible. Progenies of plants with intermediate reactions always segregated. Although Kyto with Pg-12 was a parent of Omega, Pg-12 was not present in any resistant line and was not involved in the Pg-a response. The respective complementary genes were isolated in stem rust susceptible lines, which when intercrossed, segregated in F2 to produce resistant plants in the expected frequencies and with the typical Pg-a phenotype. The lack of effective genetic variability for stem rust resistance among important sources of germplasm used by Australian oat breeders is a major cause for concern.  相似文献   

On-Farm Diversity Assessment and Participatory Varietal Evaluation of Cold-Tolerant Rice in Mid-Hills of Nepal     

Yadav  Ritesh Kumar  Gurung  Rita  Dhakal  Rajeev  Adhikari  Achyut Raj  Gautam  Subash  Ghimire  Krishna Hari  Sthapit  Bhuwon Ratna 《Journal of Crop Science and Biotechnology》2019,22(5):403-414

Journal of Crop Science and Biotechnology - Drawing upon consequentially growing food insecurity in mid-hills attributed to poor adoption levels inter alia inflicted by narrow range of locally...  相似文献   

Real-time PCR Quantification and Mycotoxin Production of Fusarium graminearum in Wheat Inoculated with Isolates Collected from Potato, Sugar Beet, and Wheat     

Burlakoti RR  Estrada R  Rivera VV  Boddeda A  Secor GA  Adhikari TB 《Phytopathology》2007,97(7):835-841

ABSTRACT Fusarium graminearum causes Fusarium head blight (FHB) in small grains worldwide. Although primarily a pathogen of cereals, it also can infect noncereal crops such as potato and sugar beet in the United States. We used a real-time polymerase chain reaction (PCR) method based on intergenic sequences specific to the trichodiene synthase gene (Tri5) from F. graminearum. TaqMan probe and primers were designed and used to estimate DNA content of the pathogen (FgDNA) in the susceptible wheat cv. Grandin after inoculation with the 21 isolates of F. graminearum collected from potato, sugar beet, and wheat. The presence of nine mycotoxins was analyzed in the inoculated wheat heads by gas chromatography and mass spectrometry. All isolates contained the Tri5 gene and were virulent to cv. Grandin. Isolates of F. graminearum differed significantly in virulence (expressed as disease severity), FgDNA content, and mycotoxin accumulation. Potato isolates showed greater variability in producing different mycotoxins than sugar beet and wheat isolates. Correlation analysis showed a significant (P < 0.001) positive relationship between FgDNA content and FHB severity or deoxynivalenol (DON) production. Moreover, a significant (P < 0.001) positive correlation between FHB severity and DON content was observed. Our findings revealed that F. graminearum causing potato dry rot and sugar beet decay could be potential sources of inoculum for FHB epidemics in wheat. Real-time PCR assay provides sensitive and accurate quantification of F. graminearum in wheat and can be useful for monitoring the colonization of wheat grains by F. graminearum in controlled environments, and evaluating wheat germplasms for resistance to FHB.  相似文献   

A Distributed Lag Analysis of the Relationship Between Gibberella zeae Inoculum Density on Wheat Spikes and Weather Variables     

Paul PA  Lipps PE  De Wolf E  Shaner G  Buechley G  Adhikari T  Ali S  Stein J  Osborne L  Madden LV 《Phytopathology》2007,97(12):1608-1624

ABSTRACT In an effort to characterize the association between weather variables and inoculum of Gibberella zeae in wheat canopies, spikes were sampled and assayed for pathogen propagules from plots established in Indiana, North Dakota, Ohio, Pennsylvania, South Dakota, and Manitoba between 1999 and 2005. Inoculum abundance was quantified as the daily number of colony forming units per spike (CFU/spike). A total of 49 individual weather variables for 24-h periods were generated from measurements of ambient weather data. Polynomial distributed lag regression analysis, followed by linear mixed model analysis, was used to (i) identify weather variables significantly related to log-transformed CFU/spike (the response variable; Y), (ii) determine the time window (i.e., lag length) over which each weather variable affected Y, (iii) determine the form of the relationship between each weather variable and Y (defined in terms of the polynomial degree for the relationship between the parameter weights for the weather variables and the time lag involved), and (iv) account for location-specific effects and random effects of years within locations on the response variable. Both location and year within location affected the magnitude of Y, but there was no consistent trend in Y over time. Y on each day was significantly and simultaneously related to weather variables on the day of sampling and on the 8 days prior to sampling (giving a 9-day time window). The structural relationship corresponded to polynomial degrees of 0, 1, or 2, generally showing a smooth change in the parameter weights and time lag. Moisture- (e.g., relative humidity-) related variables had the strongest relationship with Y, but air temperature- and rainfall-related variables also significantly affected Y. The overall marginal effect of each weather variable on Y was positive. Thus, local weather conditions can be utilized to improve estimates of spore density on wheat spikes around the time of flowering.  相似文献   

Length of vernalization period affects flowering time in three lupin species     

Kedar N. Adhikari  Bevan J. Buirchell  Mark W. Sweetingham 《Plant Breeding》2012,131(5):631-636

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Bio-extraction of Potassium from Glauconite Nano-particle in an Alfisols of Southern India     

Shaheen Praveen  Tapan Adhikari  Muneshwar Singh  A. K. Patra  R. N. Singh 《Communications in Soil Science and Plant Analysis》2020,51(13):1811-1825

ABSTRACT

Glauconite is a potassium (K) bearing mineral, which generally contains 6–10% K₂O and mostly available in various natural deposits in India. Glauconite mineral was converted to nano-particle form by grinding in a high-energy ball mill. The release of K from glauconite nano-particle in soil was studied in a pot culture experiment. Five different treatments viz., 0 mg K₂O kg^?1, 100 mg K₂O kg^?1, 150 mg K₂O kg^?1, 200 mg K₂O kg^?1 used glauconite nano-particle (GNP) as a K source and 100 mg K₂O kg^?1 from muriate of potash (MOP), were applied in soil to grow maize (Zea mays L.) plant. The highest K⁺ concentration was observed in shoot at the application of 200 mg K₂O kg^?1 through GNP and recorded 2 times more K⁺ concentration in shoots as compared to 0 mg K₂O kg^?1 treatment whereas 1.1 times more than the MOP treated soil at 5^th harvesting stage. Selected plant physiological parameters namely electrical conductivity of plant cell, height, leaf area and nitrate reductase activity were also recorded maximum at 200 mg K₂O kg^?1 GNP treated soil. Overall, glauconite nano-particle could supply K throughout the growth period and enhanced biomass yield of maize plant without showing any K deficiency symptom.  相似文献   

The effect of blue and red LED light on the growth,egg production,egg quality,behavior, and hormone concentration of Hy-Line W-36 laying hens     

Ishab Poudel  Mary M. Beck  Aaron S. Kiess  Pratima Adhikari 《The Journal of Applied Poultry Research》2022,31(2):100248

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