饲用酸化剂对1～63日龄黄羽肉鸡生长性能的影响     

艾琴  方炳虎  陈瑞爱  湛穗璋  黎乃添  梁惠莲  袁照红 《广东畜牧兽医科技》2015,(3):8-12

本试验旨在评估饲用酸化剂对1~63日龄黄羽肉鸡生长性能、肠道损伤和盲肠微生物菌群变化的影响,同时探讨壳寡糖对饲用酸化剂的增效作用。试验采用单因素完全随机区组设计,设对照组(Con.)、饲用酸化剂(Trt.1)和饲用酸化剂+壳寡糖(Trt.2)共3个处理。每个处理5个重复,每个重复50羽。饲养周期为63天。试验结果表明,从零日龄开始持续在黄羽肉鸡日粮中添加饲用酸化剂、饲用酸化剂+壳寡糖后,对1~21d和22~42d阶段黄羽肉鸡生长性能的影响未达显著(P0.05)水平;但在43~63d阶段时,与对照组相比,添加饲用酸化剂、饲用酸化剂+壳寡糖组的耗料增重比分别降低0.11和0.14,具有极大的生产意义;且饲用酸化剂添加组盲肠大肠杆菌/乳酸杆菌值显著(P0.05)低于对照组,饲用酸化剂+壳寡糖添加组盲肠大肠杆菌/乳酸杆菌值显著(P0.05)低于对照组和饲用酸化剂添加组。说明饲用酸化剂可通过维持肠道健康,促进养分消化吸收,从而提高饲料利用率;而壳寡糖在平衡肠道菌群、维持肠道健康方面对饲用酸化剂有增效作用。  相似文献   

Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation     

Masato HIYAMA  Ken Takeshi KUSAKABE  Ai TAKESHITA  Sinji SUGI  Nobue KUNIYOSHI  Hiroyuki IMAI  Kiyoshi KANO  Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):305-311

LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.  相似文献   

湖北省三峡地区天然林生态效益评估     

黄继红  黄志霖  艾训儒  姚兰  郭仲军  路兴慧 《湖北林业科技》2015,44(2):1-4,49

本文采用涵养水源、保育土壤、固碳释氧、积累营养物质、净化大气环境和生物多样性保护等9个功能指标,对湖北省三峡地区天然林生态系统服务功能价值进行评估。结果表明:湖北省三峡地区天然林生态系统服务价值为336.66亿元·年-1。其中,固碳释氧、涵养水源和生物多样性保护3项功能价值量最大,分别占该地区天然林生态系统服务总价值的34.94%,30.45%和29.00%;阔叶林生态系统服务功能价值量最大,占该地区天然林生态系统服务总价值的37.54%。固碳释氧、涵养水源和生物多样性保护等功能都发挥着重要的作用,阔叶林在湖北省三峡地区天然林生态系统中具有突出的功能。  相似文献   

哀牢山保护区景东片区中山湿性常绿阔叶林特点研究     

王春华  谢有能  周梓楠  刘国庆  鲁成荣  艾倩 《林业科技情报》2015,47(2):16-19

哀牢山位于云南省中部,保护区分布在哀牢山的中上部,其中景东片区面积为11 812hm2,位于哀牢山西坡北段。此次我们在哀牢山保护区主要保护对象之一的中山湿性常绿阔叶林中设置了样地,开展详细调查,并对调查数据进行了计算、统计、分析。通过各个样本乔木层、灌木层、草本层的H’值比较,发现乔木层﹥灌木层﹥草本层,表明乔木层的信息量大于灌木层,而草本层是该结构中相对较低的。  相似文献   

Identification of ‘Candidatus phytoplasma ziziphi’ associated with persimmon (Diospyros kaki Thunb.) fasciation in China          下载免费PDF全文

J. Wang  R. Gao  X. M. Yu  M. An  Z. H. Qin  J. Liu  C. X. Ai 《Forest Pathology》2015,45(4):342-345

Persimmon (Diospyros kaki Thunb.) trees, with fasciation symptoms (PF), were observed in an orchard located in suburban Tai'an, Shandong Province, China. A specific fragment of the phytoplasma 16S rRNA gene, approximately 1.2 kb in length, was amplified from two symptomatic plants via nested polymerase chain reaction, while no fragment was obtained from healthy controls. The two samples (PF1 and PF2) resulted with 99.5% nucleotide sequence identity. Phylogenetic and restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences revealed that PF1 was a member of ribosomal subgroup B of the elm yellows group (16SrV), and PF2 may represent a novel subgroup within the 16SrV group, designed as 16SrV‐I. This is the first report of phytoplasmas of the 16SrV group associated with persimmon fasciation disease.  相似文献   

Localization of putative pituitary stem/progenitor cells in female dairy cattle     

Ai OGUCHI  Masashi HIGUCHI  Yoshiaki YAMANO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1031

Research on sex-determining region Y-box 2 (SOX2)-positive pituitary stem/progenitor cells, as a source of hormone-producing cells, is progressing rapidly in rodents. However, the stem/progenitor cells supplying hormone-producing cells that are essential for growth, reproduction, and lactation in bovines have not yet been identified. In this study, we characterized SOX2-positive cells in the pituitary gland of dairy cattle (Holstein heifers) after sexual maturity. Immunofluorescence analysis revealed that the localization pattern of SOX2-positive cells in the dairy cattle pituitary gland was similar to that observed in the rodent pituitary gland; the marginal cell layer (MCL), dense cell clusters, and single cells scattered in the parenchyma of the anterior lobe. Furthermore, most of the SOX2-positive cells were positive for the pituitary stem/progenitor cell niche markers E-cadherin and cytokeratin 8+18, which have been reported in rodents. In addition, in the MCL of the anterior lobe, there was a subpopulation of SOX2-positive cells positive for paired-related homeobox 1 and 2, whereas negative for S100β. Moreover, in the parenchyma of the anterior lobe, co-localization of SOX2 and pituitary hormones was infrequent. In summary, this study reveals the localization of putative pituitary stem/progenitor cells positive for SOX2 in dairy cattle. These results provide valuable information to support further investigation of cell supply in the dairy cattle pituitary gland.  相似文献   

龙南遗址红烧土植物蛋白石分析     

郑云飞  游修龄 《中国水稻科学》1994,8(1):55-56

龙南遗址红烧土植物蛋白石分析郑云飞，游修龄，徐建民，边其均（浙江农业大学，杭州３１００２９）ＰｌａｎｔＯｐａｌＡｎａｌｙｓｉｓｏｆＬｏｎｇｎａｎＮｅｏｌｉｔｈｉｃＳｉｔｅ￥ＺＨＡＮＧＹｕｎｆｅｉ；ＹｏｕＹｉｕｌｉｎｇ；ＸｕＪｉａｎｍｉｎ；ＢＩＡＮＱｉ...  相似文献   

30份大麦种质资源的苗期抗旱性鉴定及抗旱指标筛选     

徐银萍  潘永东  张廷红  刘梅金  任诚  姚元虎  贾延春  陈文庆  赵锋  包奇军  火克仓  牛小霞 《甘肃农业科技》2021,(9):56-67

在西北内陆地区,干旱是影响大麦生长发育的重要非生物胁迫因素,鉴定大麦资源的抗旱性,确定抗旱指标,筛选抗旱种质,培育抗旱品种具有重要意义。以30份大麦种质为材料,设置正常供水和反复干旱胁迫2个处理,在旱棚内进行盆栽试验,测定干旱对株高、根长、叶长、地上部鲜重、根鲜重、地上部干重、根干重和总干重的影响;采用抗旱性度量值(D值)、综合抗旱系数(CDC值)、加权抗旱系数(WDC)、频次分析、相关分析、主成分分析、灰色关联度分析、隶属函数分析、聚类分析和逐步回归分析相结合的方法,对其进行苗期抗旱性鉴定及抗旱指标筛选。结果显示干旱胁迫对各指标均有极显著影响。频次分析表明,各指标对干旱胁迫反应的敏感程度依次为根长、根干重、总干重、地上部鲜重、地上干重、叶长、根鲜重和株高;相关分析表明,总干重与根干重、地上部干重、叶长和根长呈极显著正相关,与地上部鲜重呈显著正相关,与株高和根鲜重呈不显著正相关;主成分分析表明,4个主成分可代表大麦种质资源抗旱性83.583%的原始数据信息量。基于D值、CDC值和WDC值的供试大麦种质抗旱性排序相近。灰色关联度分析表明,各指标DC值与D值间的关联度大小依次为株高、地上部干重、总干重、叶长、地上部鲜重、根长、根干重、根鲜重,与各指标DC值与WDC值关联度大小排序基本吻合。根据D值进行聚类分析,可将供试大麦种质划分为5个抗旱级别,其中1级2份、2级13份、3级6份、4级7份、5级2份。除根鲜重、地上部干重、根干重和总干重外,其余指标的隶属函数值、CDC值、D值和WDC值均随抗旱级别的升高而增大。逐步回归分析表明,与D值密切相关的指标有株高和总干重。苗期抗旱性强的大麦种质资源材料有西藏25和NEVADA,可作为大麦抗旱育种、抗旱机理及干旱调控缓解机制研究的材料。株高和总干重可作为评价大麦种质资源苗期抗旱性鉴定的性状指标。  相似文献   

Status of Biological Studies and Aquaculture Development of the Mud Crab, <Emphasis Type="Italic">Scylla serrata</Emphasis>, in China: An Experimental Ecological Studies     

Wang?Gui-zhongEmail author  Li?Shao-jing  Zeng?Chao-shu  Lin?Shu-jun  Kong?Xiang-hui  Ai?Chun-xiang  Lin?Qiong-wu 《Aquaculture International》2005,13(5):459-468

This is an initial paper in a series of overviews of biological research and aquaculture development of the mud crab, Scylla serrata, in China. Results of experimental ecological studies on mud crabs are reported here. As a result of these experimental studies, results that are important for mud crab culture were also discovered and these include, methods to condition and manage broodstock, determination of ecological conditions that are suitable for embryonic development, and the influence of temperature, salinity, diet and larval density on development and survival of larvae. Results of this work will be useful in establishing a good method for artificial mass culture of larvae.  相似文献   

安徽理工大学生活污水水质监测与评价     

阮淑娴  艾慧颖  陈俊  范廷玉 《绿色科技》2014,(3):68-69

以安徽理工大学校园生活污水为研究对象,分别对校园3个主要污水排放区进行了生活污水样品采集和城市污水中CODcr 、BOD5、N H3-N、T P、SS等主要水质指标监测分析。结果显示：校园生活污水基本满足城市二级污水处理厂的污水排放要求,其中水体生化需氧量和化学需氧量高,水体存在富营养化的趋势。  相似文献