Identification and evaluation of chloroplast uni- and trinucleotide sequence repeats in citrus     

Ziniu Deng  Stefano La Malfa  Yuming Xie  Xingyao Xiong  Alessandra Gentile 《Scientia Horticulturae》2007

A cpDNA fragment of 34 genotypes belonging to Citrus and four related genera was amplified and sequenced. Chloroplast microsatellites were revealed with the length of repeats ranging from 25 to 44 bases. Other than the normal uninucleotide poly(A) repeats, a trinucleotide poly(TAA) motif was also found, the first report of such repeats in a plant chloroplast genome. According to SSR structure variations, 18 Chloroplast SSR Types (CST) were identified. The CST sequences were informative for better understanding the genetic relationships of chloroplast genomes among the analyzed genotypes and confirmed some previous hypotheses about the female parent of several hybrid accessions.  相似文献   

hCG is more effective than the GnRH agonist buserelin for inducing the first ovulation of the breeding season in mares     

Diana Fanelli  Matteo Tesi  Alessandra Rota  Massimiliano Beltramo  Giuseppe Conte  Mario Giorgi  Giovanni Barsotti  Francesco Camillo  Duccio Panzani 《Equine veterinary journal》2022,54(2):306-311

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A new kaurane diterpene dimer from Parinari campestris     

Braca A  Abdel-Razik AF  Mendez J  Morelli I 《Fitoterapia》2005,76(7-8):614-619

A new kaurane diterpene dimer, 15-oxozoapatlin-13alpha-yl-10'alpha,16'alpha-dihydroxy-9'alpha-methyl-20'-nor-kauran-19'-oic acid gamma-lactone-17'-oate (1), together with the known 13-hydroxy-15-oxozoapatlin (2), 10alpha,13alpha,16alpha,17-tetrahydroxy-9alpha-methyl-15-oxo-20-nor-kauran-19-oic acid gamma-lactone (3), 2alpha,10alpha,13alpha,16alpha,17-pentahydroxy-9alpha-methyl-15-oxo-20-nor-kauran-19-oic acid (19,10)-lactone (4), 3alpha,10alpha,13alpha,16alpha,17-pentahydroxy-9alpha-methyl-15-oxo-20-nor-kauran-19-oic acid gamma-lactone (5), and 1beta,16alpha,17-trihydroxy-ent-kaurane (6) were isolated from the leaves of Parinari campestris and identified on the basis of detailed spectral analysis, including 2D NMR spectrometry and ESI-MS.  相似文献   

A new acylated quercetin glycoside from Ranunculus lanuginosus     

Maria Prieto J  Braca A  Morelli I  Barker A  Schaffner U 《Fitoterapia》2004,75(6):533-538

A new acylated quercetin glycoside, quercetin 3-O-(2-t-p-coumaroyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), together with the known 3-(3,4-dihydroxyphenyl)lactic acid (2) were isolated from Ranunculus lanuginosus leaves and identified on the basis of detailed spectral analysis, including 2D-NMR spectrometry and ESI-MS.  相似文献   

Canine cutaneous melanocytic tumours: significance of β‐catenin and survivin immunohistochemical expression          下载免费PDF全文

Laura Bongiovanni  Alessandra D'Andrea  Ilaria Porcellato  Andrea Ciccarelli  Daniela Malatesta  Mariarita Romanucci  Leonardo Della Salda  Luca Mechelli  Chiara Brachelente 《Veterinary dermatology》2015,26(4):270-e59

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Evaluation of 2-alkylcyclobutanones in irradiated cured pork products during vacuum-packed storage     

Zanardi E  Battaglia A  Ghidini S  Conter M  Badiani A  Ianieri A 《Journal of agricultural and food chemistry》2007,55(10):4264-4270

The 2-Alkylcyclobutanones (2-ACBs) content was determined in three Italian cured pork products (salame Milano, coppa, and pancetta) irradiated at different targeted irradiation doses (2, 5, and 8 kGy) during vacuum-packed storage. Among 2-ACBs, three different compounds were investigated, namely, 2-dodecylcyclobutanone, 2-tetradecylcyclobutanone, and 2-(tetradec-5'-enyl)cyclobutanone. 2-ACBs were absent from the nonirradiated samples, whereas their content increased with irradiation dose. Their presence was recorded occasionally at 2 kGy and constantly at higher irradiation doses (5 and 8 kGy). The plot of 2-ACBs content against targeted irradiation doses showed an exponential relationship. The effect of vacuum-packed storage time on the 2-ACBs content was dependent on the irradiation dose. During vacuum-packed storage for up to 60 days, the 2-ACBs content remained unchanged in the cured pork products irradiated at 2 and 5 kGy, whereas a significant increase was observed in the pork products irradiated at 8 kGy.  相似文献   

Supercritical CO2 extract of Cinnamomum zeylanicum: chemical characterization and antityrosinase activity     

Marongiu B  Piras A  Porcedda S  Tuveri E  Sanjust E  Meli M  Sollai F  Zucca P  Rescigno A 《Journal of agricultural and food chemistry》2007,55(24):10022-10027

The volatile oil of the bark of Cinnamomum zeylanicum was extracted by means of supercritical CO2 fluid extraction in different conditions of pressure and temperature. Its chemical composition was characterized by GC-MS analysis. Nineteen compounds, which in the supercritical extract represented >95% of the oil, were identified. (E)-Cinnamaldehyde (77.1%), (E)-beta-caryophyllene (6.0%), alpha-terpineol (4.4%), and eugenol (3.0%) were found to be the major constituents. The SFE oil of cinnamon was screened for its biological activity about the formation of melanin in vitro. The extract showed antityrosinase activity and was able to reduce the formation of insoluble flakes of melanin from tyrosine. The oil also delayed the browning effect in apple homogenate. (E)-Cinnamaldehyde and eugenol were found to be mainly responsible of this inhibition effect.  相似文献   

Studies on the phytotoxicity of acid smuts     

Giacomo Lorenzini  Alessandra Panattoni  Giorgio Schenone 《Water, air, and soil pollution》1988,42(1-2):47-56

Experiments were carried out to investigate, under controlled conditions, the effects of acid smut emitted by oil-fired power plants. The foliar response of 31 selected plant species was ranked and marked differences in sensitivity were observed. Anatomical features of the foliar lesions were described. The toxicity of smuts was largely dependent on their acidity and size. Toxicity was related to temperature but was completely independent of relative humidity in the range from 50 to 90%. No interactive effects were observed between acid smuts and some commercial fungicides, or treatments with asymptomatic concentrations of So₂ in fumigation chambers. Some growth parameters were negatively affected by acid smuts in radish.  相似文献   

Chemical composition and glycemic index of Brazilian pine (Araucaria angustifolia) seeds     

Cordenunsi BR  De Menezes Wenzel E  Genovese MI  Colli C  De Souza Gonçalves A  Lajolo FM 《Journal of agricultural and food chemistry》2004,52(11):3412-3416

The seeds of Parana pine (Araucaria brasiliensis syn. Araucaria angustifolia), named pinh?o, are consumed after cooking and posterior dehulling, or they are used to prepare a flour employed in regional dishes. Native people that live in the South of Brazil usually consume baked pinh?o. As a result of cooking, the white seeds become brown on the surface due to the migration of some tinted compounds present in the seed coat. In this work, the proximate composition, minerals, flavonoids, and glycemic index (GI) of cooked and raw pinh?o seeds were compared. No differences in moisture, lipids, soluble fiber, and total starch after boiling were found. However, the soluble sugars and P, Cu, and Mg contents decreased, probably as a consequence of leaching in the cooking water. Also, the boiling process modified the profile of the phenolic compounds in the seeds. No flavonols were detected in raw pinh?o seeds. The internal seed coat had a quercetin content five times higher than that of the external seed coat; also, quercetin migrated into the seed during cooking. The internal seed coat had a high content of total phenolics, and seeds cooked in normal conditions (with the seed coat) showed a total phenolics content five times higher than that of seeds cooked without the seed coat. Cooking was then extremely favorable to pinh?o seeds bioactive compounds content. The carbohydrate availability was evaluated in a short-term assay in humans by the GI. The GI of pinh?o seeds cooked with the coat (67%) was similar to that of the seeds cooked without a coat (62%) and lower than bread, showing that cooking does not interfere with starch availability. The low glycemic response can be partly due to its high content of resistant starch (9% of the total starch).  相似文献   

Nitrogen and carbon mineralisation of leather meal in soil as affected by particle size of fertiliser and microbiological activity of soil     

Maria?Teresa?Dell'AbateEmail author  Anna?Benedetti  Alessandra?Trinchera  Daniele?Galluzzo 《Biology and Fertility of Soils》2003,37(2):124-129

Carbon and nitrogen mineralisation of leather meal fertilisers were studied in two soils characterised by different respiration activity. Both C and N mineralisation were highest in the most active soil, and when leather meal was added as a powder rather than as 2- to 4- and 4- to 6-mm particles. Fast and slow soluble N pools were determined after extraction with cold water and with hot buffer, respectively. The N remaining after the second extraction with hot buffer was named slow-release N. The percentage of slow-release N rose as the size of the applied leather meal particles increased, whereas fast soluble N was highest in the coarsest (4-6 mm) fertiliser.  相似文献