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51.
According to the United Nations Convention to Combat Desertification (UNCCD), soil erosion is one of the main causes of desertification in the Northern Mediterranean. Recently, the role of channelled erosion in sediment delivery has been underlined by several studies. The aim of this paper is to describe a simplified experimental methodology settled up for studying the relationships between gully erosion and land use.The experimental site, which is wide about 720 ha, is located in a hilly area threatened by desertification in Central Eastern Sardinia (Italy). This area is characterised by irregular morphology with steep slopes, where agropastoral activities, particularly tillage with heavy machinery and overgrazing, are causing severe soil erosion and compromising agricultural productivity. Previous studies carried out in the same area highlighted the need to quantify and monitor soil erosion in order to outline management guidelines for farmers. In this study, an integrated methodology that uses aerial photographs and field surveys was applied to inventory the gullies of the area. The degree of activity and morphological characteristics of gullies were assessed in the field by measuring the position and shape of gully heads, and their width and depth. Total gully volume was also estimated. Relationships among distribution, density and morphological characteristics of gullies, and geomorphological features, land use and land use historical dynamics of the area have been studied. The results show the very significant impact of agricultural activities on gully activity and density.  相似文献   
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The evaluation criteria applied on a 34 set of minor and main olive varieties of a restricted and climatically disfavoured region of Italy have allowed to clearly identify some local varieties with valuable agro-ecological adaptation and outstanding oil quality traits. These traits are necessary for the establishment and/or improvement of olive culture in a disadvantaged cultivation area. The molecular and morphological analyses have allowed to establish the identity of local unknown ecotypes, establishing their relationships either with other local varieties or with wide-spread cultivars, while the evaluation of oil quality parameters ranked their suitability for further exploitation.  相似文献   
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The development of a rapid detection method for Xanthomonas campestris pv. campestris (Xcc) in crucifer seeds and plants is essential for high-throughput certification purposes. Here we describe a diagnostic protocol for the identification/detection of Xcc by PCR amplification of fragments from the pathogenicity-associated gene hrcC. Under stringent conditions of amplification, a PCR product of 519 bp from hrcC was obtained from a collection of 46 isolates of Xcc, with the exception of two isolates from radish. No amplicons were obtained from 39 pure cultures of the phytopathogenic bacteria Xanthomonas campestris pv. cerealicola, X. campestris pv. juglandis, X. campestris pv. pelargonii, X. campestris pv. vitians, X. arboricola pv. pruni, X. axonopodis pv. phaseoli, X. axonopodis pv. vesicatoria, X. vesicatoria, Pseudomonas syringae pv. phaseolicola, P. syringae pv. syringae, P. syringae pv. tomato, P. fluorescens, P. marginalis, Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum. In addition, PCR reactions were negative for fifty unidentified environmental isolates purified from the surface of crucifers. The PCR fragment was obtained from four strains previously classified as X. campestris pv. aberrans, X. campestris pv. armorociae, X. campestris pv. barbarae and X. campestris pv. incanae using pathogenicity assays. Our PCR protocol specifically detected Xcc in inoculated leaves, seeds and naturally infected leaves of crucifers.  相似文献   
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Xylella fastidiosa subspecies pauca strain De Donno (XfDD) ST53 is the causal agent of olive quick decline syndrome, a severe disease first described in Apulia, Italy. Although the two local cultivars Cellina di Nardò and Ogliarola Salentina showed high susceptibility, traits of resistance to the bacterium were found in the cultivar Leccino. Previous studies in field-grown olives suggested that vascular occlusions and anatomophysiological properties of the different cultivars played a role in the olive response to XfDD. The present investigation reports observations at the early stage of the infection on artificially inoculated olives. Electron microscope studies showed that XfDD exploits the pit membranes (PMs) of the susceptible cultivar Cellina di Nardò to spread systemically. In this cultivar, PMs were degraded upon XfDD infection, suggesting activity of bacterial cell wall-degrading enzymes. Moreover, occluded vessels contained an amorphous electrondense matrix resembling gum. Conversely, in Leccino, occluded vessels were mainly filled by callose-like granules that tightly entrapped XfDD cells. In addition, PMs from Leccino had a compact undegraded structure that was not permeable to XfDD. Our study suggests that exploitation of PMs is a key event in the infection process of X. fastidiosa subsp. pauca ST53 in susceptible olive cultivars.  相似文献   
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The reproductive performances of silver European eel in term of gonad development and egg production, employing slow‐release implants with the androgen 17‐MT (1 mg) in combination with traditional weekly injection of carp pituitary extract (CPE) was evaluated. Wild female European eels (Anguilla anguilla) underwent a standard induction protocol with CPE and were randomly divided into three groups (N‐group, no implant; Y‐group, with implant; and control, C‐group, no treatment). The results showed that 17‐MT‐treated females (Y‐group) reproduced spontaneously about 6 weeks earlier than the N‐group females with a saving of almost 40% in CPE and time of induction. Concerning artificial induction of maturation in female silver eels, our study demonstrated that they positively respond to androgen exposure also in terms of eggs productivity. Indeed, Y‐group was more productive than N‐group: in Y‐group, 11 eels ensured an eggs production that exceeded 50% of initial body weight (BW), whereas in N‐group only three eels have exceeded this value. The results suggest that 17‐MT should be considered in future protocols for the improvement of the artificial reproduction of female silver European eels.  相似文献   
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  1. The worldwide decline of marine forests, due to human impacts and climate change, emphasizes the need to develop and implement effective and sustainable solutions to restore these endangered habitats and to re-establish the services they provide.
  2. In this study, the ex situ restoration of Gongolaria barbata (=Treptacantha barbata), a Mediterranean subtidal habitat-forming species of brown seaweed, was for the first time implemented in a marine protected area in the Adriatic Sea. Two restoration efforts were performed in 2019. The first was started in winter, after a marine heatwave that triggered early fertility, and the second in spring, when the species usually reproduces.
  3. This study aimed to evaluate: (i) the disruptive effects of a thermal anomaly on the reproductive biology and performance in culture of G. barbata; and (ii) the impact of the grazing pressure on juveniles after the outplanting.
  4. The first cultivation was more productive than the second, in terms of zygote release and germling growth. To mitigate the low efficiency of the second culture and to avoid prolonged highly demanding maintenance in the mesocosms, the cultivation period was extended outdoors using a structure suspended in the water column.
  5. The modular frames conceived for outplanting G. barbata proved to be effective because of their easy operability and low cost. Controlling for herbivorous fish had significant positive effects on both juvenile survival and growth.
  6. The outcomes highlighted that an unpredictable climatic event and fish grazing were major threats that impaired the restoration process of G. barbata. These stressors should be considered when developing plans to implement effective large-scale restoration of canopy-forming macroalgae.
  相似文献   
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