Identification and in planta detection of Pseudomonas syringae pv. tomato using PCR amplification of hrpZ_Pst     

Massimo Zaccardelli  Annalisa Spasiano  Carlo Bazzi  Massimo Merighi 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(1):85-90

A rapid detection method based on PCR amplification of Pseudomonas syringae pv. tomato chromosomal sequences was developed. Primer design was based on the P. syringae DC3000 hrpZ_Pst gene, which maps on a pathogenicity-associated operon of the hrp/hrc pathogenicity island.A 532 bp product corresponding to an internal fragment of hrpZ_Pst was amplified from 50 isolates of P. syringae pv. tomato belonging to a geographically representative collection. The amplification product was also obtained from three coronatine-deficient strains of P. syringae pv. tomato.On the other hand, PCR did not produce any such products from 100 pathogenic and symbiotic bacterial strains of the genera Pseudomonas, Xanthomonas, Erwinia, and Rhizobium and 75 unidentified bacterial saprophytes isolated from tomato plants. The method was tested using leaf and fruit spots from naturally-infected tomato plants and asymptomatic nursery plants and artificially contaminated tomato seeds. The results confirmed the high specificity observed using pure cultures.  相似文献   

What is your diagnosis? Unusual urinary crystals from a cat          下载免费PDF全文

Joseph Cyrus Parambeth  Annalisa M. Hernandez  Mary B. Nabity  Karen E. Russell  Alexandra N. Myers  Melanie L. Puchot  Kate E. Creevy  Jonathan A. Lidbury 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(3):507-508

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Time trends and risk factors for diabetes mellitus in cats presented to veterinary teaching hospitals     

Prahl A  Guptill L  Glickman NW  Tetrick M  Glickman LT 《Journal of Feline Medicine and Surgery》2007,9(5):351-358

Veterinary Medical Data Base records of cats with diabetes mellitus (DM) from 1970 through 1999 were reviewed to identify trends in hospital prevalence of DM and potential host risk factors. Hospital prevalence increased from eight cases per 10,000 in 1970 to 124 per 10,000 in 1999 (P < 0.001). Case fatality percent at first visit decreased from 40% to 10% (P < 0.001). Hospital prevalence increased in all age groups (P < 0.002). There was no apparent seasonal pattern in hospital prevalence. Significant risk factors included male gender, increasing age for both genders (P < 0.001), increasing weight for males (P < 0.001), and mixed vs pure breed for females (P = 0.006).  相似文献   

Long-Term Monitoring of Oxygen Saturation at Altitude Can Be Useful in Predicting the Subsequent Development of Moderate-to-Severe Acute Mountain Sickness     

Gaia Mandolesi  Giovanni Avancini  Manuela Bartesaghi  Eva Bernardi  Luca Pomidori  Annalisa Cogo 《The Forestry Chronicle》2014,25(4):384-391

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Viridepyronone, a new antifungal 6-substituted 2H-pyran-2-one produced by Trichoderma viride     

Evidente A  Cabras A  Maddau L  Serra S  Andolfi A  Motta A 《Journal of agricultural and food chemistry》2003,51(24):6957-6960

A new antifungal 6-substituted 2H-pyran-2-one, named viridepyronone, has been isolated from a cultural filtrate of a strain of Trichoderma viride showing antagonistic activity in vitro toward Sclerotium rolfsii, which is the causal agent of crown and stem rot of artichoke. Viridepyronone was characterized as 6-(4-oxopentyl)-2H-pyran-2-one 2 with spectroscopic methods. Bioassays showed that viridepyronone had a good antifungal activity against S. rolfsii, and its minimum inhibitory concentration (over 90% inhibition) was found to be 196 microg/mL. This is the first report of viridepyronone produced by any species of fungi.  相似文献   

Compared use of HPLC and FZCE for cluster analysis of Triticum spp and for the identification of T. durum adulteration     

Bonetti A  Marotti I  Catizone P  Dinelli G  Maietti A  Tedeschi P  Brandolini V 《Journal of agricultural and food chemistry》2004,52(13):4080-4089

Wheat quality criteria continually evolve in response to market pressure and consumer preference. Characterization of cereal cultivars for quality and agronomic properties, have widely shown the importance of the protein content to ensure good quality products. The aim of this work is a comparison of reversed-phase high performance liquid chromatography (RP-HPLC) and free zone capillary electrophoresis (FZCE) in the identification of Italian wheat cultivars and detection of durum wheat flour adulteration. Mainly alcohol soluble (gliadins) and water soluble (albumins) proteins were extracted from 14 common wheat cultivars and from 9 durum wheat cultivars. In RP-HPLC chromatograms, wheat albumins and gliadins eluted between 3 and 9 min and between 10 and 42 min, respectively. Even if the chosen chromatographic conditions (reversed phase) did not permit a complete resolution of hydrophilic proteins such as albumins, a good reproducibility was observed for both albumins and gliadins. In FZCE electropherograms, wheat albumins and gliadins migrated between 8 and 14 min and 16-25 min, respectively. A good reproducibility was found for wheat albumins, while the relatively poor reproducibility of gliadin fractions was a consequence of the selected separation conditions aimed to separate in the same run either hydrophilic (albumins) and alcohol-soluble (gliadins) proteins. The principal component analysis (PCA) of HPLC and FZCE data evidenced that both techniques allowed the univocal identification of the great proportion of investigated wheat cultivars. Three peaks were exclusively detected in RP-HPLC chromatograms of common wheat cultivars, while three unique peaks were found in FZCE electropherograms of common wheat cultivars. These peaks were investigated as a basis for detecting and estimating the adulteration of durum wheat flour with flour from common wheat. The direct relationship between the area of the peaks and adulteration level enabled standard curves to be constructed. The standard curves showed that adulteration may be quantified by either RP-HPLC or FZCE.  相似文献   

FT-IR study of dopant-wool interactions during PPy deposition     

Alessio Varesano  Annalisa Aluigi  Claudio Tonin  Franco Ferrero 《Fibers and Polymers》2006,7(2):105-111

Coating the fibre surface byin situ oxidative chemical polymerisation of polypyrrole (using FeCl₃ as oxidant) is a readily industrial applicable way to give electrical properties to wool with good ageing stability [1], although pre-treatments are required to avoid damage of the cuticle surface due to the acidic condition of the process. FT-IR and EDX analysis reveal that organic sulphonates and sulphates, used as dopants, are absorbed by wool, while chlorine ions are preferably embedded on the polypyrrole layer. The resulting electrical conductivity seems mainly due to the presence of chlorine as counter-ion of polypyrrole; nevertheless, the presence of arylsulphonate in the polymerisation bath increases the electrical conductivity of the coating layer.  相似文献   

Chemometrical characterization of four italian rice varieties based on genetic and chemical analyses     

Brandolini V  Coïsson JD  Tedeschi P  Barile D  Cereti E  Maietti A  Vecchiati G  Martelli A  Arlorio M 《Journal of agricultural and food chemistry》2006,54(26):9985-9991

This paper describes a method for achieving qualitative identification of four rice varieties from two different Italian regions. To estimate the presence of genetic diversity among the four rice varieties, we used polymerase chain reaction-randomly amplified polymorphic DNA (PCR-RAPD) markers, and to elucidate whether a relationship exists between the ground and the specific characteristics of the product, we studied proximate composition, fatty acid composition, mineral content, and total antioxidant capacity. Using principal component analysis on genomic and compositional data, we were able to classify rice samples according to their variety and their district of production. This work also examined the discrimination ability of different parameters. It was found that genomic data give the best discrimination based on varieties, indicating that RAPD assays could be useful in discriminating among closely related species, while compositional analyses do not depend on the genetic characters only but are related to the production area.  相似文献   

Viridenepoxydiol, a new pentasubstituted oxiranyldecene produced by Trichoderma viride     

Evidente A  Cabras A  Maddau L  Marras F  Andolfi A  Melck D  Motta A 《Journal of agricultural and food chemistry》2006,54(18):6588-6592

A new pentasubstituted oxiranyldecene, named viridenepoxydiol, has been isolated (0.9 mg/L) from the culture filtrate of a strain of Trichoderma viride showing in vitro and in vivo antagonistic activity against Sclerotium rolfsii, which is the causal agent of crown and stem rot of artichoke. Viridenepoxydiol was characterized as 3,5,9-trimethyl-2-oxiranyl-dec-8-ene-2,5-diol (3) using spectroscopic methods. It showed inhibitor effect on mycelial growth of S. rolfsii and its minimum inhibitory concentration (over 90% inhibition) was found to be 396 mug/mL. This is the first time that viridenepoxydiol was reported.  相似文献   

The 70-kDa Heat-shock Protein as a Potential Biomarker of Quality of the Parapenaeus longirostris Shrimp Flesh     

Annalisa Pinsino  Caterina Costa  Roberta Russo  Francesca Zito  Valeria Guarrasi 《Journal Of Aquatic Food Product Technology》2018,27(1):122-130

The deep-water rose shrimp, Parapenaeus longirostris, is a target species of the Mediterranean fisheries mostly caught by trawlers offshore, processed, and frozen on board. The effects of thawing on shrimp muscle exudate collected at 0, 1, 2, 3 days after thawing were investigated. In total, 70-kDa heat-shock protein (Hsp70), alpha (α)-enolase, and manganese superoxide dismutase (Mn-SOD) were selected as metabolic and stress-related proteins and analyzed by immunoblotting on exudates. Data were compared for the amount of exudates collected and the pH values. Among the investigated proteins, only the Hsp70 levels showed a decrease related to the post-thawing period and correlated with both the significant increase of the exudate amount and the pH values. These data strongly suggest the potential use of Hsp70 as an early predictive biomarker for quality of the P. longirostris shrimp after thawing.  相似文献