Outbreaks of pythiosis in two flocks of sheep in northeastern Brazil     

Tabosa IM  Riet-Correa F  Nobre VM  Azevedo EO  Reis-Júnior JL  Medeiros RM 《Veterinary pathology》2004,41(4):412-415

Two outbreaks of cutaneous pythiosis caused by Pythium insidiosum were diagnosed in two herds of crossbred hair wool sheep of different ages in the semiarid region of Northeastern Brazil. In one herd of 120 sheep, 40 were affected. The other outbreak affected six sheep out of 80. Local swellings with ulcerative lesions were observed in the limbs and abdominal and prescapular regions. Three sheep were necropsied. Two of them had lung metastasis characterized by multifocal nodules measuring 0.5-2 cm. In one animal, the prescapular lymph node was also affected. In another, the cutaneous lesion extended to the sesamoid bone. Microscopically, there were multifocal granulomas with intralesional P. insidiosum hyphae and Splendore-Hoeppli material surrounding the hyphae. The diagnosis was based on the histologic lesions, immunohistochemical identification, and culture of the etiologic agent. One sheep treated with potassium iodide recovered. Standing in swampy water for long periods in a warm aquatic environment seems to be the reason for the high prevalence of the disease.  相似文献   

Performance of the Santa Ines breed raised on pasture in semiarid tropical regions and factors that explain trait variation     

Adriana de Farias Jucá  Juliana Cantos Faveri  Geraldo Magalhães Melo Filho  Antônio de Lisboa Ribeiro Filho  Hymerson Costa Azevedo  Evandro Neves Muniz  Luís Fernando Batista Pinto 《Tropical animal health and production》2014,46(7):1249-1256

This study aimed to evaluate sex, the number of lambs per birth, and the family effects on production traits in the Santa Ines breed of sheep by estimating the least square means and coefficient of variance for those traits. A total of 484 lambs were evaluated for the following traits: weight at birth, at weaning, and at 240 days of age; weight gain during the pre-weaning and post-weaning periods; height, width, and length of different body regions; and rib eye area and fat thickness between the 12th and 13th ribs. We observed coefficients of variation higher than 10 % for several traits. Generally, males were larger than females (P?P?P?ANOVA.  相似文献   

Supervised independent component analysis as an alternative method for genomic selection in pigs     

C.F. Azevedo  F.F. Silva  M.D.V. de Resende  M.S. Lopes  N. Duijvesteijn  S.E.F. Guimarães  P.S. Lopes  M.J. Kelly  J.M.S. Viana  E.F. Knol 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2014,131(6):452-461

The objective of this work was to evaluate the efficiency of the supervised independent component regression (SICR) method for the estimation of genomic values and the SNP marker effects for boar taint and carcass traits in pigs. The methods were evaluated via the agreement between the predicted genetic values and the corrected phenotypes observed by cross‐validation. These values were also compared with other methods generally used for the same purposes, such as RR‐BLUP, SPCR, SPLS, ICR, PCR and PLS. The SICR method was found to have the most accurate prediction values.  相似文献   

Genotype selection by REML/BLUP methodology in a segregating population from an interspecific Passiflora spp. crossing     

Eileen Azevedo Santos  Alexandre Pio Viana  Josie Cloviane de Oliveira Freitas  Daniele Lima Rodrigues  Rulfe Ferreira Tavares  Claudia Lougon Paiva  Margarete Magalhães Souza 《Euphytica》2015,202(1):1-11

  相似文献   

Regulation of lysine metabolism and endosperm protein synthesis by the opaque-5 and opaque-7 maize mutations   总被引：3，自引：0，他引：3  

Azevedo RA  Lea PJ  Damerval C  Landry J  Bellato CM  Meinhardt LW  Le Guilloux M  Delhaye S  Varisi VA  Gaziola SA  Gratão PL  Toro AA 《Journal of agricultural and food chemistry》2004,52(15):4865-4871

Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.  相似文献   

Chemicals with sweet aroma descriptors found in Portuguese wines from the Douro region: 2,6,6-trimethylcyclohex-2-ene-1,4-dione and diacetyl     

Rogerson FS  Castro H  Fortunato N  Azevedo Z  Macedo A  De Freitas VA 《Journal of agricultural and food chemistry》2001,49(1):263-269

  相似文献   

Postpartum toll-like receptors and β-defensin 5 mRNA levels in the endometrium of Holstein cows     

Martins Tda M  da Paixão TA  Costa ÉA  Pires Ade C  Santos RL  Borges ÁM 《Veterinary immunology and immunopathology》2011,139(2-4):277-281

Toll-like receptors (TLRs) and β-defensins are important components of the innate immune system. This study aimed to evaluate endometrial mRNA levels of TLRs (1/6, 2, 4, and 5) and β-defensin 5 in Holstein cows by quantitative real time RT-PCR. Uterine biopsies were performed from 6 to 12 h after parturition, and cows were divided into two groups: (i) cows with placental retention and clinical signs of uterine infection until 45 days postpartum (n=10) or (ii) cows with normal puerperium (n=10). All cows had detectable levels of TLRs and β-defensin 5 mRNAs, but these levels did not differ between groups (P>0.05). Levels of TLR4 mRNA had a positive and significant correlation with the time required for uterine involution in both groups.  相似文献   

Strain-dependent arthritogenic potential of the zoonotic pathogen Corynebacterium ulcerans     

Dias AA  Silva FC  Santos LS  Ribeiro-Carvalho MM  Sabbadini PS  Santos CS  Filardy AA  Myioshi A  Azevedo VA  Hirata R  Villas-Bôas MH  Mattos-Guaraldi AL 《Veterinary microbiology》2011,153(3-4):323-331

During the last decade the majority of diphtheria cases in Europe had Corynebacterium ulcerans as the etiologic agent with dogs and cats as the reservoir hosts. However, little has been documented about the virulence factors of this zoonotic pathogen. To set up an in vivo experimental C. ulcerans infection model, conventional Swiss Webster mice were intravenously infected with different doses (from 1 × 10(7) to 5 × 10(9) bacteria per mouse) of C. ulcerans strains, namely 809 (from human lower respiratory tract), BR-AD22 (from asymptomatic dog nares) and CDC-KC279. Mortality rates were demonstrated by LD(50) values ranging from 1.9 × 10(8) to 1.3 × 10(9). Viable bacteria were recovered from blood, kidneys, liver, spleen and joints. For CDC-KC279 and 809 strains (2 × 10(8)mL(-1)) approximately 85% and 72% of animals with articular lesions were observed, respectively; BR-AD22-infected mice showed no signs of arthritis. CDC-KC279 and 809 strains exhibited higher arthritogenic potential when compared to the homologous toxigenic (ATCC27012) and non-toxigenic (ATCC27010) strains of Corynebacterium diphtheriae. A high number of affected joints and arthritis index in addition to the histopathological features, including subcutaneous edema, inflammatory infiltrate, damage to bone tissue and synoviocyte hypertrophy, indicated a strain-dependent ability of C. ulcerans strains to cause severe polyarthritis. A correlation between the arthritis index and systemic levels of IL-6 and TNF-α was observed for C. ulcerans strains, with the exception of the non-arthritogenic BR-AD22 strain. In conclusion, C. ulcerans revealed a strain-dependent arthritogenic potential independent of DNAse, PLD and diphtheria toxin production.  相似文献   

Transfer of maternal cytokines to suckling piglets: in vivo and in vitro models with implications for immunomodulation of neonatal immunity     

Nguyen TV  Yuan L  Azevedo MS  Jeong KI  Gonzalez AM  Saif LJ 《Veterinary immunology and immunopathology》2007,117(3-4):236-248

Maternal cytokines may play instructive roles in development of the neonatal immune system. However, cytokines in colostrum and milk and their transfer from mothers to neonates have not been well documented, except for TGF-beta. Swine provide a unique model to study lactogenic cytokines because the sow's impermeable placenta prohibits transplacental passage. We investigated IL-6 and TNF-alpha (pro-inflammatory), IFN-gamma and IL-12 (Th1), IL-10 and IL-4 (Th2) and TGF-beta1 (Th3) concentrations in sow serum and colostrum/milk and serum of their suckling and weaned piglets and in age-matched colostrum-deprived gnotobiotic piglets. All cytokines were detected in colostrum/milk and correlated with concentrations in sow serum except for mammary-derived TNF-alpha and TGF-beta1. Detection of IL-12 and TGF-beta1 in pre-suckling and colostrum-deprived gnotobiotic piglet serum suggests constitutive production: other cytokines were undetectable confirming absence of transplacental transfer. Peak median cytokine concentrations in suckling piglet serum occurred at post-partum days 1-2 (IL-4>IL-6>IFN-gamma>IL-10). The effects in vitro of physiologically relevant concentrations of the two predominant lactogenic cytokines (TGF-beta1 and IL-4) on porcine naive B cell responses to lipopolysaccharide (LPS) and rotavirus (RV) were investigated. High (10 ng/ml) TGF-beta1 suppressed immunoglobulin secreting cell responses to LPS and rotavirus; low concentrations (0.1 ng/ml) promoted isotype switching to IgA antibody. Interleukin-4 induced inverse dose-dependent (0.1 ng>10 ng/ml) isotype switching to IgA and enhanced IgM secreting cell responses to LPS and rotavirus. In summary, we documented the transfer and persistence of maternal cytokines from colostrum/milk to neonates and their potential role in Th-2 biased IgA responses and reduced immunologic responsiveness of neonates.  相似文献   

From the laboratory to the field: efficacy of entomopathogenic nematodes to control the cattle tick     

Marcos Daniel Gomes Filgueiras  Renata Silva Matos  Lucas Prado Barreto  Gabriel Moura Mascarin  Pricila Vetrano Rizzo  Fernanda Mara Cunha Freitas  Márcia Cristina de Azevedo Prata  Caio Monteiro  Éverton Kort Kamp Fernandes 《Pest management science》2023,79(1):216-225

  相似文献