阿月浑子砧木研究进展   总被引：3，自引：0，他引：3  

路丙社  董源  刘忠华  白志英 《果树学报》1999,16(2):1

综合介绍了世界阿月浑子砧木资源、自然分布、栽培表现、育苗及繁殖技术及其研究进展。  相似文献   

阿月浑子结果特性研究现状   总被引：1，自引：0，他引：1  

路丙社  白志英  董源  刘中华 《果树学报》1999,(Z1)

介绍了国外阿月浑子大小年结果的研究进展,包括以下几个方面：（１） 花序芽分化及其脱落;（２） 树体对大小年结果的响应;（３） 激素与花序芽脱落;（４） 大小年结果的控制措施;（５） 大小年结果机理和存在的问题  相似文献   

阿月浑子砧本研究进展     

路丙社  董源  刘忠华  白志英 《果树学报》1999,(2)

综合介绍了世界阿月浑子砧木资源、自然分布、栽培表现、育苗及繁殖技术及其研究进展。  相似文献   

猴头菌提取物对大鼠胃粘膜损伤保护作用的研究   总被引：7，自引：0，他引：7  

杨焱  唐庆九  周昌艳  白韵琴  严慧芳  陆宏琪 《食用菌学报》1999,(1)

用三种不同主式得到的猴头菌提取物(猴头菌子实体粗多糖、猴头菌菌丝体粗多糖、猴头菌菌丝体全提物)对乙醇致大鼠胃粘膜损伤的治疗作用进行研究。结果表明,当以等原生药量计时,子实休机多糖疗效最明显,菌丝体全提物次之,菌丝体粗多糖较差,溃疡抑制率分别达70.8％、51.4％和41.5％;当以等多糖含量计时,则三个样品的疗效接近,溃疡抑制率达到60％左右。这进一步证实猴头菌多糖是猴头菌保护大鼠胃粘膜的主要活性物质。  相似文献   

Effect of Discontinuous Administration of β -glucan and Glycyrrhizin on the Growth and Immunity of Pacific White Shrimp Litopenaeus Vannamei     

Nan Bai  Kangsen Mai  Wenbing Zhang  Hongming Ma  Qinghui Ai  Xiaojie Wang 《饲料工业》2010,(3):5-6

A six-week growth trial was conducted to compare the effects of different feeding strate- gies of dietary immunostimulants on the growth and immunity of white shrimp Litopenaeus vannamei （4.70 ±0.20g）. Shrimps were fed with diet containing glycyrrhizin continuously, containing β -glucan continuously, discontinuously （seven days with diet containing β -gluseven days with diet without -glucan; two days with diet containing β-glucan following five days with diet without -glucan）,  相似文献   

马正常肢体血流图实验研究     

赵生才  白景煌 《黑龙江畜牧兽医》1992,(12):1-3

本实验首次采用XLJ—8型桥式血流图仪描记了12匹健康马四肢血流图.结果表明,马肢体血流图描记法是可行的,同时提供了健康马肢体血流图8项主要指标的参考值。本实验的首试成功,为肢体血流图在兽医临床领域中广泛应用奠定了基础.  相似文献   

嗜水气单胞菌疫苗的检验及免疫效力试验   总被引：3，自引：1，他引：3  

范红结  黄国庆 《畜牧与兽医》1998,30(6):252-253

选用嗜水气单胞菌（Ａｅｒｏｍｏｎａｓｈｙｄｒｏｐｈｉｌｉｃ,Ａｈ）疫苗生产用菌Ｊ－１株,以气升式发酵罐２８℃通气培养２８ｈ,制备嗜水气单胞菌疫苗用菌液。半成品检验表明,该疫苗用菌液内无杂菌生长,细菌数量达２．７×１０１０ｃｆｕ／ｍｌ。以０．４％福尔马林溶液３７℃灭活２４ｈ,制成灭活疫苗。经成品检验,该疫苗为棕黄色液体,涂布平板无活菌生长,以１×１０７ｃｆｕ攻击６尾健康鲫鱼,１周内鲫鱼无异常反应。取该疫苗分别以腹腔注射和浸泡两种方式各免疫１２尾鲫鱼,１月后以同源强毒菌株攻击,疫苗的相对存活率分别为８３．３％和５８．３％。  相似文献   

猪繁殖与呼吸综合征病毒相关猪microRNA的初步筛选     

郜原  薄联锋  武小椿  孙柏  温俊歌  孙岩  徐志坤  张德礼 《中国兽医杂志》2012,48(3):21-24

本试验旨在初步筛选出与猪繁殖与呼吸综合征病毒(PRRSV)感染相关的猪microRNA(miRNA)。采用生物信息学方法预测可能与PRRSV 3′非编码区(3′utr)产生相互作用的猪miRNA,构建PRRSV 3′utr的双荧光素酶报告基因载体psi-CHECK-utr,同时合成预测到的miRNA(ssc-miR-323、ssc-miR-105-1)及其相应的抑制物,共转染猪脐静脉血管内皮细胞系(SUVEC),检测双荧光素酶活性。结果发现,ssc-miR-323与psiCHECK-utr共转染后,荧光比率略有升高,ssc-miR-105-1与psiCHECK-utr共转染后,荧光比率降低。初步判定ssc-miR-323对PRRSV 3′utr没有抑制作用,而ssc-miR-105-1对PRRSV3′utr有一定的抑制作用。  相似文献   

中药"病毒克"颗粒剂对人工感染新城疫鸡肝功能指标的影响     

尹秀玲  牛发良  白迎春  张玉 《中国家禽》2006,28(3):15-17

试验用75只1日龄罗曼鸡人工感染新城疫后,饲料中按每千克体重添加“病毒克”颗粒剂0．5g、1g治疗组和0.5g预防组,并设阳性对照组和健康对照组。结果表明：血清总蛋白（TP）0．5g治疗组极显著高于阳性对照组（P〈0．01）;白蛋白（ALB）各治疗组极显著高于阳性对照组,预防组显著高于阳性对照组;r-谷氨酸转移酶（GGT）和胆固醇（CHO）阳性对照组显著低于治疗组;乳酸脱氢酶（HDBH）阳性对照组显著高于各治疗组和预防组等,表明药剂对肝功能有较好的保护作用。  相似文献   

The 16MΔvjbR as an ideal live attenuated vaccine candidate for differentiation between Brucella vaccination and infection     

Wang Y  Bai Y  Qu Q  Xu J  Chen Y  Zhong Z  Qiu Y  Wang T  Du X  Wang Z  Yu S  Fu S  Yuan J  Zhen Q  Yu Y  Chen Z  Huang L 《Veterinary microbiology》2011,151(3-4):354-362

Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development.  相似文献