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101.
102.
Biological and molecular characterisation of ten Peanut stunt virus (PSV) isolates from Robinia pseudoacacia was carried out. The host range of these isolates was similar to that of the previously described PSV strains in most cases, but on Pisum sativum and Lens culinaris latent infection was induced. Variability in systemic symptoms was observed only on Nicotiana glutinosa. The partial RNA3 sequences were determined, including the carboxyl terminal region of the movement protein gene, the intergenic region, the entire coat protein gene and the 3′ untranslated region. Nucleotide sequence comparison of the coat protein genes showed 77.6–84.2% identity with most of the known PSV strains and 96.3–98.0% identity with PSV-Rp the typical member of subgroup IV. Phylogenetic analysis indicated the presence of the ancient homologous recombination in all of the examined black locust isolates and all the isolates were members of the fourth PSV subgroup. These results showed that the isolates of the fourth subgroup are widely distributed in black locust in this region.  相似文献   
103.
The characteristics of the growth and formation of meat production of first-generation bulls of a new Volga type of Red and White breed obtained by using Dutch-bred Holstein Red and White gene pool are studied.  相似文献   
104.
Immobilizing materials such as lherzolite could reduce metal bioavailability but the effectiveness of lherzolite on the extractability and bioavailability of cadmium (Cd) and zinc (Zn) is rarely investigated. We conducted a greenhouse experiment to investigate the effect of 5% application of lherzolite to a contaminated soil on the chemical fractionation of Cd and Zn and their uptake by radish (Raphanus sativus L.) and Japanese mustard spinach (Brassica rapa L. var. perviridis). Both plants were grown in a highly contaminated (with Cd and Zn) sandy loam soil. Plants were cultivated consecutively three times in the same pots. After the third cultivation, soil samples were collected and analyzed by sequential extraction procedure into five operationally defined fractions (F1—exchangeable, F2—carbonate-bound, F3—oxides-bound, F4—bound with organic matter, and F5—residual). Addition of lherzolite to soil decreased 50% of exchangeable (F1) Cd but it increased the carbonate (F2), oxide (F3), and organic (F4) fraction Cd. For Zn, application of lherzolite resulted into the reduction of both F1 (87%) and F2 (33%) fractions but it increased the F3, F4, and F5 fractions. The reduction in exchangeable fraction of Cd and Zn in the soil resulted in higher plant growth and lower concentrations of both Cd (64% to 92%) and Zn (78% to 99%) in plant tissues of both plant species grown. We may thus conclude that application of lherzolite resulted into lower availability of these metals in the soil leading to lower uptake of Cd and Zn by plant roots, lower toxicity, and ultimately higher plant growth.  相似文献   
105.
The distribution half-life, elimination half-life, apparent volume of distribution and total body clearance of carbenicillin in healthy buffalo calves following a single intravenous administration (50 mg/kg) were 0.057±0.005 h, 1.688±0.11 h, 0.185±0.021 L kg-1 and 75.97±6.519 ml kg-1 h-1 respectively. A satisfactory dosage regimen for carbenicillin in buffalo calves was calculated to be 56 mg/kg followed by 52 mg/kg body weight repeated at 6 h intervals.  相似文献   
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Vascular complications are major causes of graft failure in liver transplantation. The use of different vascular grafts is common but the results are controversial. The aim of this study was to create an 'ideal' arterial interponate for vascular replacements in the clinical field. An autologous, tubular graft prepared from the posterior rectus fascia sheath was used for iliac artery replacement in dogs for 1, 3, 6 and 12 months. Forty-one grafts were implanted and immunosuppression was used in separate groups. The patency rate was followed by Doppler ultrasound. Thirty-seven grafts remained patent, 2 cases with thrombosis and 2 cases with stenosis occurred. There was no evidence of necrosis or aneurysmatic formation. The histological analysis included conventional light microscopic and immunohistochemical examinations for CD34 and factor VIII. The explanted grafts showed signs of arterialisation, appearance of elastin fibres, and smooth muscle cells after 6 months. Electron microscopy showed intact mitochondrial structures without signs of hypoxia. In conclusion, the autologous graft presents acceptable long-term patency rate. It is easy to handle and the concept of beneficial presence of the anti-clot mesothelium until endothelialisation seems to work. The first clinical use was already reported by our group with more than 2 years survival.  相似文献   
109.
Foliar sprayings of grapevines with different plant growth regulators or chemicals were evaluated for determining grape skin color and phenolic compounds content and enhancing table grape quality characteristics of cv. Red Globe in this study. Grape skin color is one of the most important quality factors for table grapes. Sometimes, it can be viewed difficulties in skin coloration of some red table grape cultivars grown in various grape growing regions of the world and poor skin coloration of red table grapes is a frequent trouble that reduces production efficiency. Despite the fact that it is utilized from some canopy management practices; plant growth regulators, chemicals with plant hormones and hormone-like compounds as active ingredients can be recently used for improving anthocyanin accumulation in growing of some table grape cultivars. In current study, nine different preharvest treatments were used for improving quality parameters of cv. Red Globe, including 1?Control (C), 2–300?mg/L Abscisic acid (ABA), 3–300?mg/L Ethephon (Eth), 4–30% Ethanol (EtOH), 5–300?mg/L ABA + 300?mg/L Eth, 6–300?mg/L ABA + 30% EtOH, 7–300?mg/L Eth + 30% EtOH, 8–300?mg/L ABA + 300?mg/L Eth + 30% EtOH and 9?Cluster tip cutting (CTC). Although the best results from total phenolic compounds content and total anthocyanin content of cv. Red Globe were obtained in CTC treatment in all preharvest treatments, Eth+ETOH treatment especially gave rise to the best results among the preharvest chemical treatments.  相似文献   
110.
Field evolution of CH4 and CO2 from soils under four dominant land uses in the Mardi watershed, western Nepal, were monitored at 15-day intervals for 1 year using closed chamber techniques. The CH4 oxidation rate (mean±SE, g CH4 m–2 h–1) in the forest (22.8±6) was significantly higher than under grazing land (14±2) and an upland rainfed maize and millet system (Bari) (2.6±0.9). Irrigated rice fields (Khet) showed an oxidation rate of 6±0.8 g CH4 m–2 h–1 in the dry season (December–May) but emitted a mean rate of 131 g CH4 m–2 h–1 in the rainy season and autumn (June–October). The evolution of CO2 ranged from 10 mg CO2 m–2 h–1 in the Bari in January to 1,610 mg CO2 m–2 h–1 in the forest in July. Higher evolution of CO2 (mean±SE, mg CO2 m–2 h–1) was observed in the Bari (399±39) and forest (357±36) compared to Khet (246±25) and grazing (206±20) lands. The annual emission of CO2 evolution varied from 86.6 to 1,836 g CO2 m–2 year–1. The activation energy for CH4 and CO2 varied between 16–283 and 80–117 kJ mol–1, respectively. The estimated temperature coefficient for CO2 emission varied from 2.5 to 5.0. Temperature explained 46–51% of the variation in CO2 evolution, whereas it explained only 4–36% of the variation in CH4 evolution.  相似文献   
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