高分子树脂膜培养容器在文心兰试管苗培养中的应用   总被引：7，自引：3，他引：7  

何松林  任凝辉  潘会堂  杨秋生  张启翔  田中道男 《北京林业大学学报》2002,24(4):93-96

以低成本、高品质试管种苗培养和规模化生产为目的,采用具有高透气性、低透湿性、耐高温和易于成型加工的四碳氟乙烯树脂膜为材料制作新型培养容器（CP）,以文心兰'Aloha Iwanaga'为实验植物材料,在常规培养条件下探讨了树脂膜培养容器在文心兰试管苗生产中的有效性.与传统培养容器三角瓶相比较,CP内生长的试管苗在株高、叶长、叶幅、根数、根长、鲜重、干物重、干物率和叶绿素指数等生长指标上高于常规培养容器（三角瓶）.采用SSR法进行比较（显著水平P≤0.05）,其中在株高、叶长、叶幅、鲜重、干物重等重要生长指标之间存在显著性差异.实验结果表明,使用CP作为培养容器对促进文心兰试管苗的生长和提高其品质效果明显.  相似文献   

马铃薯抑芽剂CIPC的抑芽效果     

冯双庆  魏明峰  苑秋妍  俞毅路  张凌云 《中国农业大学学报》1995,(2):171-174

马铃薯抑芽剂 CIPC(isoprophyl chloro-carbanilate)在刚出休眠期及采后2个月的薯块上应用都有明显的抑芽效果。在常温下141d 发芽率为10%,抑芽效果与低温贮藏的相似。CIPC 与杀菌剂混合使用可以减轻腐烂,CIPC 与多菌灵混用的效果最好。  相似文献   

长白山森林沼泽生态交错带群落演替规律研究(Ⅰ)——白桦沼泽群落演替规律研究     

牟长城  韩士杰  罗菊春  王襄平 《北京林业大学学报》2001,23(1)

该文目的在于揭示长白山森林沼泽生态交错带群落的演替规律 ,以便为科学地保护和经营管理森林与湿地过渡带生物资源提供依据 .选择本区典型的白桦沼泽生态交错带为研究对象 .采用定量观测和建立经验回归模型的方法 ,研究群落建群种白桦生长过程和斑块动态、蒸腾排水作用的相关规律 ,预测白桦沼泽生态交错带演替进程 .研究结果表明 :随着群落建群种白桦生长加快、年龄不断增大 ,林分个体着生的微域斑块呈现三次式函数曲线增大 ,林分蒸腾排水作用按幂函数曲线增强 .随着群落的发育 ,斑块不断扩大和生物排水作用的增强 ,白桦沼泽交错群落在 5 0～ 6 0a内将会使交错带生境趋于中生化 ,并演替为森林  相似文献   

中国大蚊属四新种(双翅目:大蚊科)     

杨定  杨集昆 《中国农业大学学报》1995,(1):90-93

本文记述了我国大蚊属(Tipula Linnaeus)4新种及1新记录种,模式标本存北京农业大学昆虫标本室。  相似文献   

啤酒花α-酸、β-酸及主要香精油成分的研究     

李正应  郑国华  邢卫兵  李光晨  潘季淑 《中国农业大学学报》1993,(S5):31-37

该项研究分析测定了23个啤酒花品种的α—酸、β—酸和香精油的组成特性。结果表明:不同品种其α—酸和β—酸的组成是不同的,香型品种含合葎草酮的比例较低,相反,苦型品种含合葎草酮的比例较高;同一品种种植在不同地区其α—酸和β—酸组成特性也不同,且各品种的表现不一致;与苦型啤酒花相比,香型啤酒花品种在组成啤酒花香精油的三种主要成分中,葎草烯和石竹烯含量较高、而香叶烯的含量较低;同一品种种植在不同地区其香精油组成也有所不同。α—酸和香精油的组成特性是评价啤酒花酿造特性的重要指标,香型啤酒花的α—酸中葎草酮含量常在60%以上,合葎草酮含量常在25%以下,香精油中葎草烯与石烯竹含量之和大于香叶烯。  相似文献   

电子束辐射诱变及克隆选择培育好好芭抗寒无性系的研究   总被引：6，自引：1，他引：6  

张根发  王舒静  胡伯继  张桂芳  石小明  王纯 《中国油料作物学报》2003,25(4):56-59

利用电子辐射处理好好芭试管苗，从改变基因型入手提高其抗寒力，以获得稳定遗传的好好芭株系。研究表明150GY的辐射量是诱发好好芭发生变异的最佳剂量，结合生化标记，抗寒相关的酶类——淀粉酶和过氧化物酶活性分析，人工模拟低温实验的克隆检测，筛选出了具有较高抗寒能力的好好芭株系，这些株系通过无性系繁殖可用于抗寒育种。  相似文献   

Asia I口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达(英文)     

张韵  张金卫  易咏竹  李志勇  李江涛  鱼南洋  丁农  柳纪省 《（《农业科学与技术》）编辑部》2011,(10)

[目的]研究AsiaI口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达,以期筛选出较适合表达该组合基因的家蚕品种。[方法]将已经获得高效表达的重组家蚕杆状病毒rBmNPV(P1-2A3C)基因,分别注射原种及杂交家蚕蚕蛹。利用ELISA方法对其进行抗原表达量检测,并对表达结果进行差异比较。[结果]不同家蚕品种对rBmNPV(P1-2A3C)的表达存在着明显差异;秋丰×TQ78和秋丰×丝胶茧杂交组合可考虑作为高效表达的家蚕生物反应器专用品种。[结论]为AsiaIFMDV目的蛋白的高效表达专用品种的选育提供了依据。  相似文献   

茎瘤固氮根瘤菌Azorhizobium caulinodansORS571基因组分泌蛋白的生物信息学分析     

王新新  吴亮  朱生凤  李广茹  赵林  吕妍  李飞雪  陈宇 《广西农业生物科学》2012,(4):394-400

为了获取茎瘤固氮根瘤菌（AzorhizobiumcaulinodansORS571）的分泌蛋白，以便更深入地了解该菌的共生固氮作用，本研究采用SignalP、TMHMM、PSORTb、TargetP、LipoP、TatP和SecretomeP软件对该菌全部4717个蛋白序列进行分析预测。结果共识别了653个分泌蛋白，其中具有分泌型信号肽的蛋白54个，具有RR—motif型信号肽的蛋白1个，具有脂蛋白信号肽的蛋白2个和非经典分泌蛋白596个。该菌含信号肽分泌蛋白仅占全部蛋白的1．2％，低于其它固氮菌。在分泌蛋白中识别了核酸内切酶和核糖核酸酶等6个核酸酶。它们可能参与宿主植物遗传物质的降解，干扰宿主遗传代谢，进一步在宿主植物侵染过程中起到重要作用。此外还识别了超氧化物歧化酶、过氧化氢酶和谷胱甘肽S-转移酶等4个抗氧化酶。它们可能参与活性氧的清除以保护固氮酶，是该菌固氮过程的重要参与者。  相似文献   

Influence of gallic acid on porcine neutrophils phosphodiesterase 4,IL-6,TNF-α and rat arthritis model     

JIANG Dai-xun  ZHANG Mei-hua  ZHANG Qian  CHEN Yi-shan  MA Wen-jing  WU Wei-peng  MU Xiang  CHEN Wu 《中国农业科学(英文版)》2015,(4):758-764

Our previous studies showed that the anti-inflammatory effects of Paeonia lactiflora roots extract may be mediated, at least in part, through its gallic acid content, and this effect may be regulated in part by an inhibition on c AMP-phosphodiesterase(PDE). To explore the anti-inflammatory effect and mechanism, the influence of gallic acid on neutrophils PDE4 activity and expression, TNF-α and IL-6 content and rat arthritis model were further studied. PDE4 activity and gene express were calculated respectively by substrate c AMP change examined with HPLC and real-time RT-PCR. The concentration of IL-6 and TNF-α in supernatant were assayed by ELISA method. Model of rat arthritis was caused by complete Freund’s adjuvant. Results showed that gallic acid had a dose-dependent restraint on PDE4 activity of neutrophils in vitro, promoted significantly PDE4 A expression(P<0.01), and had no influence on the expressions of PDE4 B and 4D. However, PDE4 C expression was not detected. Gallic acid could promote IL-6 release(P<0.05), and inhibit TNF-α release of neutrophils(P<0.05). The experiment in vivo showed that gallic acid had obvious restraint on local inflammation of animal model(P<0.05). Therefore, the anti-inflammatory effect of gallic acid may be mediated in part through an inhibition on PDE4 activity and further an increase of IL-6 and a decrease of TNF-α of neutrophils, and this effect seemed to have no relationship with PDE4 expression.  相似文献   

Molecular diversity and genetic structure of 380 sweetpotato accessions as revealed by SSR markers   总被引：2，自引：0，他引：2  

YANG Xin-sun  SU Wen-jin  WANG Lian-jun  LEI Jian  CHAI Sha-sha  LIU Qing-chang 《中国农业科学(英文版)》2015,(4):633-641

Sweetpotato, Ipomoea batatas(L.) Lam., is an important food crop widely cultivated in the world. Evaluation of genetic relationships among diverse cultivars and landraces is necessary for efficient exploitation of genetic diversity in the existing germplasm resources. In the present study, a collection of 380 sweetpotato accessions assembled from different agro-climatic zones of China and other countries were genotyped using 30 SSR primer pairs. Model-based structure analysis separated the germplasm into three populations, P1, P2 and P3, containing 228, 133 and 19 accessions, respectively, which was consistent with the results of phylogenic and principal component analysis(PCA). Analysis of molecular variance(AMOVA) revealed significant genetic differentiation among inferred populations, accounting for 16.47% of the total molecular variance, however, the differences between the regions were not significant, the total variation were due to the differences between the genotypes within the population. Pairwise fixation index(F ST) suggested that populations P1 and P3 had the highest differentiation, while populations P1 and P2 had the lowest differentiation. The diversity among populations was wide, which confirmed the genetic distinction of populations. Through comparing model-based structure and domestication-based classification, it was found that the accessions of population P1 mainly belonged to modern cultivars, and the accessions of populations P2 and P3 basically corresponded to landraces, by which we suggest that modern cultivars maybe had experienced a two-step domestication history. Our results illustrated clear genetic relationships among 380 sweetpotato accessions, exhibiting the potential of accelerating the process of future sweetpotato breeding program by molecular marker based parental selection.  相似文献