The impact of rising CO2 on ecosystem production     

Bert G. Drake 《Water, air, and soil pollution》1992,64(1-2):25-44

A fundamental property of green plants is that the rate of photosynthesis is dependent in the ambient CO₂ concentration. There is overwhelming experimental evidence that this effect increases plant production in most C₃ plants: hundreds of experiments with many species show that plant growth increases an average 30% to 40% for a doubling of the present normal ambient CO₂ concentration (Kimball, 1986). External environmental factors, such as temperature and the availability of nutrients, modify this response. The greatest stimulation of photosynthesis and growth can be expected to occur at high temperatures and much smaller responses at low temperature. Factors which restrict growth, such as low nutrients, will reduce but usually do not eliminate the stimulation of production with increasing CO₂ even when nitrogen is severly limiting. There are also reports of direct effects of ambient CO₂ concentration on dark respiration which show that there is an immediate reduction in the rate of CO₂ efflux or O₂ consumption when the CO₂ around plant tissues is increased. There have been very few longterm field studies of the effects of increased CO₂ on whole plants and ecosystem processes but the data from these studies are consistent in showing an increase in plant production with an increase in CO₂ concentration of the ambient air.  相似文献   

Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions     

László Sági  Serge Remy  Bert Verelst  Bart Panis  Bruno P. A. Cammue  Guido Volckaert  Rony Swennen 《Euphytica》1955,85(1-3):89-95

Summary In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized.Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeric uidA gene constructs by electroporation. With the inclusion of polyethylene glycol and heat shock, the frequency of transiently expressing protoplasts reached 1.8% as shown by an in situ -glucuronidase assay. A duplicated 35S promoter with an alfalfa mosaic virus leader sequence (pBI-426) induced the highest expression rate among the constructs tested.Embryogenic cell suspensions of cv. Bluggoe have also been bombarded with accelerated particles coated with a high expression uidA gene construct (pEmuGN) using a biolistic gun. After a partial optimization of the procedure, transient GUS assays reproducibly demonstrated the presence of 400 blue foci in 30 l of settled cell volume (approximately 25 mg cells). Selection and characterization of antibiotic-resistant transformed cultures is in progress.Abbreviations AMV alfalfa mosaic virus - GUS -glucuronidase - TGE transient GUS expression - uidA gene for -glucuronidase  相似文献   

Chemical composition and antioxidant activity of yerba-mate (Ilex paraguariensis A.St.-Hil., Aquifoliaceae) extract as obtained by spray drying     

Berté KA  Beux MR  Spada PK  Salvador M  Hoffmann-Ribani R 《Journal of agricultural and food chemistry》2011,59(10):5523-5527

Yerba-mate or mate? (Ilex paraguariensis A.St.-Hil., Aquifoliaceae) leaves are typically used for their stimulant, antioxidant, antimicrobial, and diuretic activity, presenting as principal components polyphenolic compounds. In this study, the objective was to develop a yerba-mate dry extract by using spray drying technology and to evaluate the dry extract antioxidant activity and chemical composition. The results obtained by means of the DPPH assay show that the extract presents an IC(50) of 2.52 mg/mL. The yerba-mate spray-dried extract presents high catalase-like activity, suggesting that it is a strong free-radical scavenger. The antioxidant activity as expressed as catalase-like activity was related to total polyphenol content. In addition, the results show that the spray-dried extract presents high polyphenol content, namely, high concentrations of caffeic acid (1.54 mg/g), 5-caffeoylquinic acid (91.40 mg/g), rutin (5.38 mg/g), and total phenolics (178.32 mg/g), which justifies its high antioxidant activity.  相似文献   

Inverse association between endotoxin exposure and canine atopic dermatitis     

Looringh van Beeck FA  Hoekstra H  Brunekreef B  Willemse T 《Veterinary journal (London, England : 1997)》2011,190(2):215-219

The development of canine atopic dermatitis (CAD) may be related to exposure to mite allergens, bacterial endotoxin and/or fungal glucans. In this study, indoor exposure levels of house dust mite allergens, endotoxins and fungal glucans were measured to determine their possible association with CAD. A case-control study including adult Labrador retrievers with (n=28) and without (controls; n=65) CAD was conducted. Dust samples were collected from the living room floor and the bedding and coat of the dog and these were analyzed for house dust mite allergens Der p1 and Der f1, endotoxin and (1→3)-β-d-glucan levels. Dog owners were also required to return a questionnaire regarding their home characteristics. The endotoxin exposure level in the coats of dogs was significantly inversely associated with CAD (odds ratio 0.38; 95% confidence interval 0.15-0.97; P<0.05). No significant difference was found in exposure levels to house dust mite allergens and fungal glucans. The results indicated that endotoxin exposure is inversely associated with CAD, suggesting a protective effect of high indoor endotoxin exposure towards the development of the condition.  相似文献   

An apparatus and technique for sampling aerially applied sprays in conifers     

Beresford L. Cadogan  Bert F. Zylstra 《Pest management science》1984,15(4):417-423

A technique and apparatus that facilitated the sampling of aerially applied pesticides on artificial surfaces in the canopy of balsam fir [Abies balsamea (L.) Mill.] and spruce (Picea spp.) are described. Field testing showed that sample units in the canopy were as receptive to droplets as those on the forest floor, and did not discernibly disturb the microenvironment in the tree.  相似文献   

The primary structure of wheat glutenin subunit 1Dx2 revealed by electrospray ionization mass spectrometry     

Bert Lagrain  Ine Rombouts  Jan A. Delcour  Peter Koehler 《Journal of Cereal Science》2014

The high molecular weight glutenin subunits (HMW-GS) play a key role in end-use quality of wheat. Their particular primary structure is mostly derived from DNA sequencing, which gives no information on potential post-translational modifications. This paper reveals the primary structure of HMW-GS 1Dx2 by proteomic analysis. For this purpose, HMW-GS were first isolated from wheat flour (cv. Contra). The relative molecular mass (M_r) of subunit 1Dx2 present in the HMW-GS mixture was then very accurately determined with high-performance liquid chromatography–electrospray ionization-mass spectrometry using a quadrupole-time-of-flight mass analyzer (HPLC–ESI-QTOF-MS). The obtained M_r value (87,105) differed from the value derived from its protein sequence in the NCBI database (87,007). The subunit was further purified by preparative reversed-phase HPLC and partially hydrolyzed with chymotrypsin. The resulting 1Dx2 peptides were then analyzed by HPLC–ESI-MS/MS and the MS data were compared to amino acid sequences in protein databases. The discrepancy between the calculated and the measured M_r of 1Dx2 was explained by a missing proline in the 1Dx2 amino acid sequence from the database and not by any post-translational glycosylation.  相似文献   

Genetics. Please don't call it cloning!     

Vogelstein B  Alberts B  Shine K 《Science (New York, N.Y.)》2002,295(5558):1237

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Understanding nano-anatomy of healthy and carious human teeth: a prerequisite for nanodentistry     

Gaiser S  Deyhle H  Bunk O  White SN  Müller B 《Biointerphases》2012,7(1-4):4

The anatomy of human teeth reflects its usage. Spatially resolved X-ray scattering permits quantitative studies of the characteristic arrangement of the anisotropic calcium phosphate crystallites and the collagen fibers within the hard tissues of the crown. The present study summarizes the distinctive nanometer-sized anatomical features of the tooth hard tissues including their interface taking advantage of spatially resolved synchrotron radiation-based small-angle X-ray scattering. The comparison of slices from eight teeth indicates a long-range organization of tooth nanostructures.  相似文献   

Pathogenicity of <Emphasis Type="Italic">Stemphylium vesicarium</Emphasis> from different hosts causing brown spot in pear     

Jürgen Köhl  Belia Groenenboom-de Haas  Helen Goossen-van de Geijn  Adrianus Speksnijder  Pieter Kastelein  Sybren de Hoog  Bert Gerrits van den Ende 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):151-162

Stemphylium vesicarium (teleomorph: Pleospora herbarum) is the causal agent of brown spot disease in pear. The species is also able to cause disease in asparagus, onion and other crops. Saprophytic growth of the fungus on plant debris is common. The objective of this study was to investigate whether isolates of S. vesicarium from different hosts can be pathogenic to pear. More than hundred isolates of Stemphylium spp. were obtained from infected pear fruits, dead pear leaves, dead grass leaves present in pear orchard lawns as well as from necrotic leaf parts of asparagus and onion. Only isolates originating from pear orchards, including isolates from dead grass leaves, were pathogenic on pear leaves or fruits in bioassays. Non-pathogenic isolates were also present in pear orchards. Stemphylium vesicarium from asparagus or onion, with one exception, were not pathogenic to pear. Analysis of the genetic variation between isolates using Amplified Fragment Length Polymorphism (AFLP) showed significant concordance with host plants. Isolates from asparagus or onion belonged to clusters separate from the cluster with isolates from pear or grass leaves collected in pear orchards. Multilocus sequencing of a subset of isolates showed that such isolates were similar to S. vesicarium.  相似文献   

Surface‐applied insecticide treatments for quarantine control of Japanese beetle,Popillia japonica Newman (Coleoptera: Scarabaeidae), larvae in field‐grown nursery plants     

Jason B Oliver  Michael E Reding  Nadeer N Youssef  Michael G Klein  Bert L Bishop  Phil A Lewis 《Pest management science》2009,65(4):381-390

BACKGROUND: Japanese beetles, Popillia japonica Newman, are a quarantine challenge for nursery shipments from infested to non‐infested states. Marathon (imidacloprid) and Discus (imidacloprid + cyfluthrin) are approved preharvest nursery treatments (US Domestic Japanese Beetle Harmonization Plan; DJHP). This study evaluated approved and non‐approved (acephate, carbaryl, clothianidin, dinotefuran, halofenozide, thiamethoxam, trichlorfon) preharvest treatments, optimal rates (labeled 1×, 0.3–0.75× or 2–3×) and optimal timings (June, July, August and September) to control early‐instar (grubs) P. japonica in field nurseries. RESULTS: Most insecticides effectively reduced grub densities, except for acephate, carbaryl and trichlorfon. Clothianidin, thiamethoxam and halofenozide provided grub control equivalent to DJHP standards during most years. Across all test years and timings, percentage grub reductions were: Marathon (1×: 59.2–100; 3×: 78.9–100), Discus (1×: 60.7–100), clothianidin (1×: 96.1–100; 3×: 97.4–100), thiamethoxam (1×: 75.0–100; 3×: 80.0–100), halofenozide (1×: 70.0–100; 3×: 90.0–100) and dinotefuran (1×: 13.2–88.2; 3×: 71.1–93.4). CONCLUSIONS: July application was more consistent and effective than other timings, and higher rates (2× and 3×) did not generally improve grub control. Overall, clothianidin, thiamethoxam and halofenozide (and dinotefuran applied in August) were equivalent to current DJHP standards. These insecticides may be suitable for DJHP Category 2 states, potentially lowering grower costs. Copyright © 2009 Society of Chemical Industry  相似文献