Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain     

RAN Xu-hua  CAO Si  ZHANG Yao  WEI Xiao-man  WEN Xiao-bo  NI Hong-bo  ZHU Zhan-bo 《中国畜牧兽医》2015,42(5):1104-1109

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.  相似文献   

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice     

LIU Lin  #  ZHANG Ying xin  #  LI Zhi  LIU Qun en  YU Ning  SUN Bin  YANG Zheng fu  ZHOU Quan  CHENG Shi hua  CAO Li yong 《中国水稻科学》2014,28(5):465

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水稻新品种龙稻19的选育及配套栽培技术     

王彤彤  孙世臣  白良明  丁国华  曹良子  周劲松  洛育  李南南 《黑龙江农业科学》2020,(8):132-134

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天门冬质量及RAPD遗传多样性研究     

徐昌艳  罗忠圣  曹旭林  钱志瑶  李相陵  覃容贵 《安徽农业科学》2021,49(3):180-184

[目的]研究天门冬质量及RAPD遗传多样性,并探索其质量与RAPD遗传多样性间相关性.[方法]采用综合评分法,以总皂苷、多糖、浸出物含量为评价指标对天门冬进行质量评价;对天门冬RAPD反应体系进行优化,完成不同居群天门冬RAPD扩增,运用NTSYS 2.10e软件进行遗传多样性分析及UPGMA系统树图构建;分析天门冬质量与RAPD遗传多样性的相关性.[结果]18份天门冬样品质量差异较大;18份天门冬多态性百分率为89.62％,遗传相似系数为0.4481～0.7486,在阈值0.52处18份天门冬聚为两大类,其中贵州遵义桐梓天门冬单独分为一类,其余聚为一类.[结论]天门冬质量与RAPD遗传多样性无显著相关性,所建立的天门冬RAPD法可用于其遗传多样性研究,可为其良种选育、规范化种植奠定基础.  相似文献   

科技成果鉴定申请表中“内容简介”的撰写研究及应用     

段华荣  王亚春  曹晨  张冰冰 《拖拉机》2014,(2):12-21

基于本单位在科技成果鉴定申请表中“内容简介”撰写时所存在的问题，就其内容的如何撰写进行了研究，其中包括“应用领域和技术原理”、“性能指标”、“与国内外同类技术比较”、“成果的创造性、先进性、作用意义”等方面的撰写研究，并通过实例进行介绍，具有可供参考借鉴的实用价值。  相似文献   

Genetic Diversity of Fruit Phenotypic Traits of Wild Pyrus ussuriensis Maxim     

AN Meng-Meng  WANG Yan-Ting  SONG Yang  JI Xiao-Hao  LIU Chang  WANG Nan  WU Yu-Sen  LIU Wen  CAO Yu-Fen  FENG Shou-Qian  CHEN Xue-Sen 《中国农业科学》2014,47(15):3034-3043

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农业机械购置补贴网络系统数据库设计与应用     

曹晓林  聂文  药林桃 《安徽农业科学》2014,(27):9627-9630

农机购置补贴是国家支农惠农的政策之一.对农机购置补贴系统的数据库功能进行分析,提出农机补贴数据表关系模型,并对系统安全维护进行论述.数据库运用在农业购置补贴网络系统中,满足农机管理部门对农机补贴数据事务性管理,为农机补贴政策决策提供实时和有效的数据分析.  相似文献   

棉隆土壤消毒防除小麦田杂草的效果及对养分的影响   总被引：1，自引：0，他引：1  

朱文达  颜冬冬  李林  曹坳程 《湖北农业科学》2020,59(6):100-103

采用田间试验研究了98%棉隆微粒剂防除小麦田主要杂草的效果,及杂草防除后对氮、磷、钾及水分的影响。结果表明,施用98%棉隆微粒剂防除小麦田杂草效果显著,对菵草(Beckmannia syzigachne)、猪殃殃(Galium aparine L)、牛繁缕[Malachium aquaticum (L.) Fries]、大巢菜(Vicia sativa L.)均有良好防效,总鲜重防效达87.5%～99.5%,优于两种对照药剂。杂草防除后,显著降低了杂草对田间氮、磷、钾和水分的消耗,有效改善了田间的水肥条件。98%棉隆微粒剂处理的小麦田产量达2 970.01～3 123.77 kg/hm2,增产效果显著,产量较清水对照增加28.9%～35.6%,每公顷增收1 532.38～1 886.02元。  相似文献   

鹌鹑ESR1基因的外显子8多态性与早期生长性能的关联分析     

樊红灯  白俊艳  付学言  曹恒  杨帅  庞有志  时坤鹏  卢小宁  马永康  陈梦柯  董智豪  安肖凯  常世增  侯伟晓  巩慧荣 《黑龙江畜牧兽医》2020,(2)

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Land surface temperature retrieval for arid regions based on Landsat-8 TIRS data:a case study in Shihezi,Northwest China   总被引：1，自引：0，他引：1  

Lei YANG  ;YunGang CAO  ;XiaoHua ZHU  ;ShengHe ZENG  ;GuoJiang YANG  ;JiangYong HE  ;XiuChun YANG 《干旱区科学》2014,(6):704-716

Scientific interest in geophysical information about land surface temperature （LST） is ever increasing, as such information provides a base for a large number of applications, including environmental and agricultural monitoring. Therefore, the research of LST retrieval has become a hot topic. Recent availability of Landsat-8 satel- lite imagery provides a new data source for LST retrieval. Hence, exploring an adaptive method with reliable ac- curacy seems to be essential. In this study, basing on features of Landsat-8 TIRS thermal infrared channels, we re-calculated parameters in the atmospheric transmittance empirical models of the existing split-window algorithm, and estimated the ground emissivity with the help of the land cover classification map of the study area. Further- more, a split-window algorithm was rebuilt by virtual of the estimation model of the updated atmospheric transmit- tance and the ground emissivity, and then a remote sensing retrieval for the LST of Shihezi city in Xinjiang Uygur autonomous region of Northwest China was conducted on the basis of this modified algorithm. Finally, precision validation of the new model was implemented by using the MODIS LST products. The results showed that the LST retrieval from Landsat-8 TIRS data based on our algorithm has a higher credibility, and the retrieved LST is more consistent with the MODIS LST products. This indicated that the modified algorithm is suitable for retrieving LST with competitive accuracy. With higher resolutions, Landsat-8 TIRS data may provide more accurate observation for LST retrieval.  相似文献