Primary Aspergillus and Fusarium keratitis in a Holstein cow     

Elligott CR  Wilkie DA  Kuonen VJ  Bras ID  Neihaus A 《Veterinary ophthalmology》2006,9(3):175-178

A 5-year-old female Holstein cow was examined for ocular discharge, periorbital swelling, an area of full-thickness corneal cellular infiltrate, fibrin, hypopyon, diffuse corneal edema, and miosis. The patient was diagnosed with a corneal stromal abscess and secondary anterior uveitis. Histopathology, mycotic culture, and polymerase chain reaction positive for Aspergillus and Fusarium DNA confirmed the presence of fungal infection. Response to therapy was adequate, and follow-up with the patient 1 year after diagnosis revealed a focal area of corneal fibrosis.  相似文献   

A critical discussion of the physics of wood–water interactions     

Emil Tang Engelund  Lisbeth Garbrecht Thygesen  Staffan Svensson  Callum A. S. Hill 《Wood Science and Technology》2013,47(1):141-161

This paper reviews recent findings on wood–water interaction and puts them into context of established knowledge in the field. Several new findings challenge prevalent theories and are critically discussed in an attempt to advance current knowledge and highlight gaps. The focus of this review is put on water in the broadest concept of wood products, that is, the living tree is not considered. Moreover, the review covers the basic wood–water relation, states and transitions. Secondary effects such as the ability of water to alter physical properties of wood are only discussed in cases where there is an influence on state and/or transition.  相似文献   

Extraocular lymphoma in the horse     

Allison T. Schnoke  Dennis E. Brooks  David A. Wilkie  Ann E. Dwyer  Andrew G. Matthews  Brian C. Gilger  Diane V. H. Hendrix  Phillip Pickett  Magda Grauwels  Christine Monroe  Caryn E. Plummer 《Veterinary ophthalmology》2013,16(1):35-42

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Actinomyces bowdenii ulcerative keratitis in a dog     

Amanda Sherman  Joshua B. Daniels  David A. Wilkie  Elizabeth Lutz 《Veterinary ophthalmology》2013,16(5):386-391

A 5‐year‐old spayed female diabetic mixed‐breed dog underwent phacoemulsification and intraocular lens implantation to correct bilateral hypermature cataracts. Two months postsurgery, the patient presented with ulcerative keratitis and multifocal stromal abscessation OD, which was controlled, but never resolved, with topical fluoroquinolone therapy. The patient re‐presented 2 months later with a new, raised, white gritty corneal opacity associated with hyperemia, chemosis, and blepharospasm OD. Cytology of the right cornea revealed filamentous bacteria, suggestive of Actinomyces spp. Actinomyces bowdenii was subsequently isolated in pure culture and identified via 16s rDNA sequencing. Actinomyces bowdenii has never before been described as a cause of ocular infection. An immunosuppressed corneal environment likely contributed to this opportunistic Actinomycosis. The infection was not controlled with fluoroquinolone therapy, and the isolate, in vitro, was resistant to three fluoroquinolones (ciprofloxacin, ofloxacin, and levofloxacin), which also has not been previously reported for this species of Actinomyces. A superficial keratectomy with conjunctival graft was employed to successfully manage the infection.  相似文献   

Porcine IgE in the context of experimental food allergy: purification and isotype-specific antibodies     

Rupa P  Hamilton K  Cirinna M  Wilkie BN 《Veterinary immunology and immunopathology》2008,125(3-4):303-314

Measurement of allergen-specific immunoglobulin E (IgE) is a common practice in the investigation of allergy. It has not been possible to measure porcine IgE due to unavailability of anti-porcine IgE. This study was undertaken to purify and characterize porcine IgE from sera of allergic pigs, identify heterologous anti-IgE reactive with pig IgE and to use purified heavy (H) chain of porcine IgE to generate rabbit anti-IgE. A four-step process for the purification of porcine IgE is reported using ammonium sulphate precipitation, Protein G affinity chromatography, DEAE cellulose anion-exchange chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) to obtain IgE H chain. The resultant IgE was evaluated for purity using SDS-PAGE and immunoreactivity was detected by Prausnitz-Küstner (PK) tests and passive cutaneous anaphylaxis with the allergen, crude peanut extract, used to induce experimental allergy. Cross-reactivity with anti-mouse and anti-human IgE antibodies were confirmed in western blot and enzyme-linked immunosorbent assays (ELISA). The H chain of IgE was excised from SDS-PAGE gels and used to develop rabbit anti-porcine IgE antisera. Antiserum obtained from rabbits immunized with porcine IgE, as well as heterologous murine and human-specific anti-IgE, induced reverse cutaneous anaphylaxis in pig skin and detected allergen-specific IgE in ELISA but did not react with IgG H chain in western blots. These results confirm allergy-associated bioactivity of porcine IgE and describe both homologous and heterologous anti-pig IgE suitable for use in allergen-specific and other assays. This will enhance utility of pig allergy models and provide an additional measure of type-2 immune response in pigs.  相似文献   

A modified ab externo approach for suture fixation of an intraocular lens implant in the dog     

Wilkie DA  Gemensky-Metzler AJ  Stone SG  Basham CR  Norris KN 《Veterinary ophthalmology》2008,11(1):43-48

The goals of canine cataract and lens instability surgery should be to ensure a small incision, minimal tissue trauma, shortened surgical time, maintenance of the anterior chamber, and restoration of emmetropia through the use of a stable intraocular lens specifically designed for the canine eye. While this is usually the case with routine phacoemulsification and in-the-bag intraocular lens implantation, it is often not the case with lens instability, lens luxation or large posterior capsular ruptures. In such cases the incisions are often larger, surgical time and tissue trauma are excessive, and the patient is often left aphakic. The goal of this paper is to present a modified ab externo technique designed to allow removal of the lens and placement of a ciliary sulcus sutured IOL through a small incision, with minimal trauma and shortened surgical time. Use of this technique may allow more canine patients to be emmetropic postoperatively. In addition, the ease of this procedure may encourage earlier removal of an unstable lens and decrease the risk of secondary glaucoma and retinal detachment that occur in association with lens luxation.  相似文献   

Use of an intravenous sclerosing foam (3% sodium tetradecyl sulfate) for treatment of orbital varix in a dog          下载免费PDF全文

Rebecca S. Saunders  Brian A. Scansen  Stephen S. Jung  Hooman Khabiri  David A. Wilkie 《Veterinary ophthalmology》2018,21(2):194-198

A 6‐month‐old intact male Standard Dachshund was referred for evaluation of a soft tissue swelling above the right eye. Examination of the right eye revealed mild lateral deviation of the globe, normal vision, and a dorsonasal soft tissue swelling. Examination of the posterior segment was normal. Dual‐phase computed tomography angiography was consistent with an orbital varix of the angularis oculi and right dorsal external ophthalmic veins with no evidence of arterial involvement. Treatment involved fluoroscopically guided coil embolization of the venous outflow with nine platinum microcoils, followed by sclerotherapy of the varix using 1.5 mL of 3% sodium tetradecyl sulfate foam. Moderate‐to‐marked swelling was noted at the treatment site in the weeks following therapy, which gradually resolved. At final reexamination 3 months post‐therapy, complete sclerosis and resolution of the orbital varix were documented. To the authors’ knowledge, this is the first reported case involving the use of a sclerotic agent for successful treatment of a venous malformation in a dog.  相似文献   

Hotspots of microbial activity induced by earthworm burrows,old root channels,and their combination in subsoil     

Duyen T. T. Hoang  Johanna Pausch  Bahar S. Razavi  Irina Kuzyakova  Callum C. Banfield  Yakov Kuzyakov 《Biology and Fertility of Soils》2016,52(8):1105-1119

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Origins of the bottom trawling controversy in the British Isles: 19th century witness testimonies reveal evidence of early fishery declines     

Ruth H Thurstan  Julie P Hawkins  Callum M Roberts 《Fish and Fisheries》2014,15(3):506-522

Bottom trawling (nets towed along the seabed) spread around the British Isles from the 1820s, yet the collection of national fisheries statistics did not begin until 1886. Consequently, analysis of the impacts of trawling on fish stocks and habitats during this early period is difficult, yet without this information, we risk underestimating the extent of changes that have occurred as a result of trawling activities. We examined witness testimonies recorded during two Royal Commissions of Enquiry (1863–66 and 1883–85). These enquiries interviewed hundreds of fishers about the early effects of sail trawling and the changes they were witnessing to fish stocks, habitats and fishing practises during this time. We converted all quantitative statements of perceived change in fish stocks and fishing practices to relative change. Witnesses from the north‐east of England interviewed during 1863 revealed an average perceived decline in whitefish of 64% during their careers, which many blamed upon trawling. Between 1867 and 1892, trawl‐landing records from the same location suggest that this trajectory continued, with fish availability declining by 66% during the period. Fishers adapted to these declines by increasing distances travelled to fishing grounds and increasing gear size and quantity. However, inshore declines continued and by the early 1880s even trawl owners were calling for closures of territorial waters to trawling in order to protect fish nursery and spawning grounds. Until now, these testimonies have been largely forgotten, yet they reveal that alterations to near‐shore habitats as a result of trawling began long before official data collection was initiated.  相似文献   

Glaucoma with Descemet's membrane detachment in five horses          下载免费PDF全文

Michala de Linde Henriksen  Noelle La Croix  David A. Wilkie  Mary Lassaline‐Utter  Karen R. Brantman  Gillian L. Beamer  Leandro B. C. Teixeira  Richard R. Dubielzig 《Veterinary ophthalmology》2017,20(3):273-279

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