The influence of the swine major histocompatibility genes (SLA) on variation in serum immunoglobulin (Ig) concentration   总被引：1，自引：0，他引：1  

B A Mallard  B N Wilkie  B W Kennedy 《Veterinary immunology and immunopathology》1989,21(2):139-151

Variation in serum IgG and IgM concentration was determined in three homozygous SLA-defined strains of miniature swine (SLAa, SLAc and SLAd) and one recombinant strain SLAg (ABcDd) as part of a study of SLA and other genetic effects on immune response. Data were obtained from 119 8-week-old piglets from 29 litters by 12 sires and analyzed using a SAS linear model for the effects of SLA haplotype, sire, dam, litter, sex, season of birth and sow parity. SLA haplotype (P less than 0.10) and other genetic effects due to sire (P less than or equal to 0.001) and dam (P less than or equal to 0.002) contributed to the variation in serum IgG concentrations. Season of birth and sow parity also affected IgG concentration as did litter effects. Least squares mean comparisons indicated that pigs of the dd, dg and gg haplotypes had significantly higher serum IgG than did pigs of the other haplotypes. Heritability estimates for IgG, calculated by paternal half-sib correlation, ranged from 0.31 to 0.27, indicating that selection for increased serum IgG concentrations would be possible. For serum IgM concentrations, only the effect of litter was significant at P less than or equal to 0.001 and P less than or equal to 0.009 by the radial immunodiffusion test read at 24 or 48 h. Since sire variance components estimates were negative, heritabilities were not calculated for IgM and are assumed to be zero.  相似文献   

Effect of lymphocytes and broncho-alveolar washing cells on the replication of bovine herpesvirus type 1 in tracheal organ cultures     

Alice Bouffard  J.B. Derbyshire  B.N. Wilkie 《Veterinary microbiology》1982,7(3):241-251

The daily addition of lymphocytes collected from a calf between 7 and 11 days after experimental infection with bovine herpesvirus type 1 (BHV-1) to bovine fetal tracheal organ cultures after infection with BHV-1 did not inhibit virus replication. The daily addition of normal lymphocytes, together with a low concentration of serum antibody against BHV-1, had a slight viral inhibitory effect which was believed to be due to antibody-dependent cell-mediated cytotoxicity. The addition of broncho-alveolar washing (BAW) cells, collected before infection or 30 days after infection of a calf with BHV-1, together with lymphocyte culture supernatant, to tracheal organ cultures immediately after infection with BHV-1 produced some inhibition of virus replication. Virus replication was markedly inhibited when BAW cells collected from the calf 18 days after infection were used in a similar manner.  相似文献   

Antibody titers to Pasteurella haemolytica A1 in Ontario beef cattle.   总被引：12，自引：7，他引：5       下载免费PDF全文

P E Shewen  B N Wilkie 《Canadian journal of veterinary research》1982,46(4):354-356

Indirect bacterial agglutination titers to Pasteurella haemolytica A1 were determined in serum, thoracic, pericardial, or peritoneal fluid from cattle necropsied as part of the Bruce County Beef Project in 1979-80 and 1980-81. Antibody titers were also assayed in serum from 84 calves on entry to feedlots in the fall of 1979. Titers on entry were low compared to antibody levels at necropsy. Cattle which died with pneumonia, in particular those dying of fibrinous pneumonia (shipping fever), had lower levels of antibody to P. haemolytica than did those dying of other causes.  相似文献   

Leukomyelitis in the Goat: A Report of Three Cases          下载免费PDF全文

I. W. Wilkie 《The Canadian veterinary journal. La revue veterinaire canadienne》1980,21(7):203-205

Three cases of focal myelitis in the spinal cords of young goats are described. The clinical findings and pathological changes were similar to those reported for viral leukoencephalomyelitis of goats. There were granular structures in a few cells in malacic areas, which on electron micrographs appear to be clumps of chromatin in the nuclei of gemistocytic astrocytes. They may represent mitotic figures.  相似文献   

Immunohistochemical characterization of a malignant intraocular teratoid medulloepithelioma in a cockatiel     

Bras ID  Gemensky-Metzler AJ  Kusewitt DF  Colitz CM  Wilkie DA 《Veterinary ophthalmology》2005,8(1):59-65

A 3-year-old cockatiel was presented for evaluation of a buphthalmic right eye (OD). The history included a traumatic event 5 months prior to presentation, and the referring veterinarian diagnosed cataract and secondary glaucoma. Computed tomography of the bird's orbital region revealed a large right orbital soft tissue mass that extended lateral to the orbital rim with obliteration of the globe without bone involvement. There was also no evidence of metastatic disease within the lungs or any abnormalities within the celomic cavity, therefore enucleation was recommended to achieve a histopathologic diagnosis and prognosis, and to palliate the clinical signs. Final diagnosis was malignant intraocular teratoid medulloepithelioma.  相似文献   

Surgical management and histologic and immunohistochemical features of a cataract and retrolental plaque secondary to persistent hyperplastic tunica vasculosa lentis/persistent hyperplastic primary vitreous (PHTVL/PHPV) in a Bloodhound puppy     

Gemensky-Metzler AJ  Wilkie DA 《Veterinary ophthalmology》2004,7(5):369-375

The objective of this study was to describe the clinical, histologic and immunohistochemical features, the surgical treatment, and outcome of a cataract secondary to persistent hyperplastic tunica vasculosa lentis/persistent hyperplastic primary vitreous (PHTVL/PHPV) in a dog. A 4-month-old male Bloodhound dog presented for evaluation of a cataract. A complete ophthalmic examination and ocular ultrasonography were performed. A resorbing cataract with intralenticular hemorrhage, lens induced uveitis, and PHTVL/PHPV were diagnosed. Extracapsular cataract extraction using phacoemulsification was performed. A primary posterior capsulectomy was performed to remove a retrolental plaque with the posterior capsule; the excised plaque was submitted for histopathology and immunohistochemical staining. A 41-Diopter intraocular lens (IOL) was implanted. Functional vision was maintained postoperatively during the 21-month follow-up period. Histologically, the posterior capsule was coiled and exhibited duplication. The retrolental plaque was comprised of dense fibrous connective tissue, blood vessels, free red blood cells, hemosiderin-laden macrophages, a pocket of neural tissue and numerous perivascular mast cells. With immunohistochemical staining, the neural elements were determined to be glial cells compatible with astrocytes. Cataract secondary to PHTVL/PHPV can be successfully treated using phacoemulsification and planned posterior capsulectomy. Posterior lens capsule duplication, mast cells and astrocytic glial cells may be normal components of the fibrovascular retrolental plaque associated with PHTVL/PHPV.  相似文献   

Evaluation of intraocular pressure in eyes of clinically normal llamas and alpacas     

Willis AM  Anderson DE  Gemensky AJ  Wilkie DA  Silveira F 《American journal of veterinary research》2000,61(12):1542-1544

OBJECTIVE: To estimate intraocular pressure (IOP) in eyes of healthy camelids, using applanation tonometry. ANIMALS: The eyes of 34 camelids (16 llamas [Lama glamal and 18 alpacas [L. pacos]) that did not have major abnormalities of the ocular surface or intraocular abnormalities. PROCEDURE: Tonometry measurements were obtained from each eye 3 times during a 24-hour period. Each measurement was the mean of several corneal applanations obtained by use of an applanation tonometer. Data were analyzed, using an ANOVA for a repeated-measures design. RESULTS: Mean (+/- SEM) IOP of llamas and alpacas was 13.10+/-0.35 and 14.85+/-0.45 mm Hg, respectively. Range of IOP was 7 to 18 mm Hg for llamas and 11 to 21 mm Hg for alpacas. Mean IOP of llamas was significantly less than the mean IOP of alpacas. Significant differences in IOP were not detected between the right and left eye of animals. Significant differences in IOP were not attributed to sex, age, or time of measurement within llamas or alpacas. CONCLUSIONS AND CLINICAL RELEVANCE: Establishing the mean and range of IOP of clinically normal llamas and alpacas provides a frame of reference that is important for use in a complete ophthalmic examination of camelids, which can assist clinicians in the diagnosis of glaucoma and uveitis. Reasons for the difference in mean IOP between llamas and alpacas are unknown. Although the difference may be unimportant clinically, this finding reiterates the fact that caution must be used when extrapolating IOP among species.  相似文献   

Disseminated haemangiosarcoma in an eastern barred bandicoot (Perameles gunnii)     

Bodley KB  Booth RJ  Samuel J  Wilkie JS 《Australian veterinary journal》2000,78(9):605-607

A captive adult male Eastern barred bandicoot (Perameles gunnii) presented with three palpable subcutaneous masses in November 1998. A diagnosis of haemangiosarcoma was made based on histological examination of one excised mass. Euthanasia of the animal was performed 11 days postsurgery and a proliferative lesion in the paralumbar musculature and similar, smaller proliferative lesions surrounding the right popliteal lymph node and in the ventricular wall of the heart were found. Metastatic lesions were found in the liver and lung. The histological features of the neoplastic tissues supported the diagnosis of a poorly differentiated, disseminated haemangiosarcoma. This is the first reported case of haemangiosarcoma in the Eastern barred bandicoot.  相似文献   

The demonstration of Pasteurella multocida in the alimentary tract of chickens after experimental oral infection     

Lee CW  Wilkie IW  Townsend KM  Frost AJ 《Veterinary microbiology》2000,72(1-2):47-55

A selective medium containing polymyxin B, crystal violet, thallous acetate, bacitracin and cycloheximide in 10% sheep blood dextrose starch agar, and a modified Pasteurella multocida-specific polymerase chain reaction (PCR) assay were developed for the respective isolation and detection of P. multocida from chicken alimentary tract. The selective medium and the PCR assay were highly sensitive, detecting 100 cfu from colon contents. These techniques were used to follow the localisation of an orally administered virulent P. multocida in chickens. Pasteurellae could be isolated from the crop of some birds up to 30 h, occasionally from other sites after 28 h. It was concluded that the crop was a likely site for colonisation and that infection was most likely to occur through the mucosa of the jejunum or ileum.  相似文献   

Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida   总被引：3，自引：0，他引：3  

Mitchison M  Wei L  Kwang J  Wilkie I  Adler B 《Veterinary microbiology》2000,72(1-2):91-96

The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A.  相似文献