Investigation of the prevalence of bovine viral diarrhoea virus in dairy cows in South Korea     

Lee DH  Park SW  Choi EW  Lee CW 《The Veterinary record》2008,162(7):211-213

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Seroprevalence of leptospirosis in animals in Korea     

Jung BY  Song YK  Choi JS  Lee KW  Ha TY  Yoon SS  Kweon CH  Park CK 《The Veterinary record》2008,163(1):28-29

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The effects of enrofloxacin on canine tendon cells and chondrocytes proliferation <Emphasis Type="Italic">in vitro</Emphasis>     

Lim S  Hossain MA  Park J  Choi SH  Kim G 《Veterinary research communications》2008,32(3):243-253

Enrofloxacin, a fluoroquinolone antibiotic has been used widely in humans and domestic animals, including dogs, because of its broad-spectrum activity and relative safety. The side effects of fluoroquinolone, induced tendinopathy, tendonitis, spontaneous tendon rupture and cartilage damage, remain incompletely understood. In the present study, we investigated the in vitro effects of enrofloxacin on cell proliferation and induction of apoptosis in canine Achilles tendon cells and chondrocytes. Cell growth and proliferation after treating with enrofloxacin for 2–6 days was quantified by a colorimetric 2,3-bis{2-methoxy-4-nitro-5-sulfophenyl}-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay. The results showed that enrofloxacin could inhibit the proliferation of canine tendon cells and chondrocytes at increasing concentrations (10–200 μg/ml). The inhibition of proliferation of canine tendon cells and chondrocytes after exposure to enrofloxacin were associated with induction of apoptosis, as evidenced by the typical nuclear apoptotic condensed nuclei found using Hoechst 33258 staining. It was demonstrated that canine tendon cells and chondrocytes treated with 200 μg/ml enrofloxacin for 4 days exhibited apoptotic features and fragmentation of DNA. Enrofloxacin also increased the apoptosis of canine tendon cells and chondrocytes in a dose and time-dependent manner. The results indicate that enrofloxacin inhibits cell proliferation, induces apoptosis and DNA fragmentation, which might explain enrofloxacin-induced tendinopathy and cartilage damage.  相似文献   

畜禽产品兽药残留超标案件的法律适用     

刘金才  李卫华  孙荣钊  张青  施佳骏  苏兰  陈向武 《中国动物检疫》2019,36(9):55-58

畜禽产品兽药残留是政府关注、社会关切和消费者关心的食品安全问题。执法实践中,执法人员对畜禽养殖、畜禽收购与屠宰、畜禽产品市场销售等环节如何适用法律存在执法困惑。本文结合有关法理分析认为:一是养殖环节销售兽药残留超标的动物或动物产品的,应根据《兽药管理条例》对养殖者进行处罚。二是屠宰环节畜禽产品兽药残留超标的,要以违法行为是否构成为判定要素。对于畜禽产品尚未销售的,相关主体没有法律责任,但应对问题畜禽产品进行无害化处理,防止用于食品消费。对于畜禽产品已销售的,应根据《农产品质量安全法》对相关违法行为人进行处罚。在自营模式和代宰模式中,违法行为人认定和法律责任归属有所不同。三是市场销售环节发现畜禽产品兽药残留超标,应由食品药品监管部门按照《食品安全法》处罚,农业执法部门应防止越权执法。  相似文献   

Ultrasonographic evaluation of renal dimension and resistive index in clinically healthy Korean domestic short-hair cats     

In-Chul Park  Hye-Sun Lee  Jong-Taek Kim  So-Jeong Nam  Ran Choi  Ki-Seok Oh  Chang-Ho Son  Changbaig Hyun 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):415-419

Renal length, height, width, resistive index (RI), size of cortex, and medulla were determined by renal ultrasonography in 50 healthy Korean domestic short-hair cats. In the sagittal plane, the renal length was 3.83 ± 0.51 cm (mean ± SD) in the left kidney and 3.96 ± 0.48 cm in the right kidney, whereas the renal height was 2.42 ± 0.27 cm in the left kidney and 2.36 ± 0.28 cm in the right kidney. In the transverse plane, the renal height was 2.42 ± 0.28 cm in the left kidney and 2.38 ± 0.27 cm in the right kidney, whereas the renal width was: 2.65 ± 0.35 cm in the left kidney and 2.63 ± 0.31 cm in the right kidney. In the dorsal plane, the renal length was 3.84 ± 0.53 cm in the left kidney and 3.97 ± 0.54 cm in the right kidney, whereas the renal width was 2.65 ± 0.34 cm in the left kidney and 2.66 ± 0.33 cm in the right kidney. There were no significant differences (p > 0.05) among the same structure sizes measured in different planes. In the sagittal plane, the size of the renal cortex was 0.47 ± 0.08 cm in the left kidney and 0.47 ± 0.08 cm in the right kidney, whereas of the size of the renal medulla was 0.55 ± 0.30 cm in the left kidney and 0.50 ± 0.07 cm in the right kidney. RI evaluated by pulsed wave Doppler sonography was 0.52 ± 0.05 in the left kidney and 0.55 ± 0.05 in the right kidney. The actual renal dimensions determined by gross examination were not statistically different from those determined by ultrasonography. Furthermore the renal dimensions and RI were statistically correlated to the body weight of cats.  相似文献   

Cytokine response in Balb/c mice infected with Francisella tularensis LVS and the Pohang isolate     

Eun-Ju Kim  Sang-Hee Park  Young-Sill Choi  Soo-Kyoung Shim  Mi-Yeoun Park  Man Suck Park  Kyu-Jam Hwang 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):309-315

We investigated the immune response induced by the Francisella (F.) tularensis live vaccine strain (LVS) and the Pohang isolate. After the Balb/c mice were infected intradermally (i.d) with 2 × 10⁴ cfu of F. tularensis LVS and Pohang, respectively, their blood and organs were collected at different times; 0, 3, 6, 24, 72, 96, 120 and 168 h after infection. Using these samples, RT-PCR and ELISA analysis were carried out for the comparative study of the cytokines, including TNF-α, INF-γ, IL-2, IL-4, IL-10 and IL-12. In the Pohang-infected mice at 120 h, the liver showed a 53 times higher level of TNF-α and a 42 times higher level of IFN-γ than the respective levels at the early time points after infection. The levels of TNF-α and IFN-γ induced by LVS were 5 times lower than those induced by the Pohang isolate. Also, the organs from the Pohang-infected mice showed higher levels of TNF-α, IFN-γ, IL-10 and IL-12 than the levels in the LVS-infected mice. The blood from the Pohang-infected mice at 120 h revealed about a 40 times increased level of IFN-γ, and IL-10 was also increased by 4 times at 96 h compared to an early infection time point, while IL-4 was not induced during the whole infection period. These results suggest that F. tularensis may induce a Th1-mediated immune response to in vivo infection and the Pohang isolate has a higher capacity than the LVS to induce an acute immune response in Blab/c mice.  相似文献   

Prevalence of feline herpesvirus 1, feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter     

Byeong-Teck Kang  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(2):207-209

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The rate of Salmonella spp. infection in zoo animals at Seoul Grand Park, Korea     

Y. H. Jang  S. J. Lee  J. G. Lim  H. S. Lee  T. J. Kim  J. H. Park  B. H. Chung  N. H. Choe 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(2):177-181

Salmonellosis is an important zoonotic disease that affects both people and animals. The incidence of reptile-associated salmonellosis has increased in Western countries due to the increasing popularity of reptiles as pets. In Korea, where reptiles are not popular as pets, many zoos offer programs in which people have contact with animals, including reptiles. So, we determined the rate of Salmonella spp. infection in animals by taking anal swabs from 294 animals at Seoul Grand Park. Salmonella spp. were isolated from 14 of 46 reptiles (30.4%), 1 of 15 birds (6.7%) and 2 of 233 mammals (0.9%). These findings indicate that vigilance is required for determining the presence of zoonotic pathogen infections in zoo animals and contamination of animal facilities to prevent human infection with zoonotic diseases from zoo facilities and animal exhibitions. In addition, prevention of human infection requires proper education about personal hygiene.  相似文献   

Serum immunoglobulin fused interferon-�� inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells     

Jun-Sung Kim  Kyeong Nam Yu  Mi Suk Noh  Min-Ah Woo  Sung-Jin Park  Jin Hong Park  Jin Hua  Hyun Sun Cho  Soon Kyung Hwang  Eun-Sun Lee  Youn-Sun Chung  In-Young Choi  Se-Chang Kwon  Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):45-50

Interferon (IFN) has therapeutic potential for a wide range of infectious and proliferative disorders. However, the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem, serum immunoglobulin has been fused to IFN. In this study, the efficacy of serum immunoglobulin fused INFs (si-IFN1 and si-IFN2) was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells, each group of mice was injected once a week with si-IFN1 and si-IFN2 at two different concentrations (10 × : 30 µg/kg and 50 × : 150 µg/kg). A slight anti-tumoral effect was observed in all 10 × groups compared to the control. In the 50 × groups, however, si-IFN1 and si-IFN2 showed significant anti- tumoral effects compared to the control. To gain more information on the mechanisms associated with the decrease of tumor size, a Western blot assay of apoptosis-related molecules was performed. The protein expression of cytochrome c, caspase 9, 6, and 3 were increased by si-IFN1 and si-IFN2. These 2 IFNs also increased the expressions of p53, p21, Bax and Bad. Interestingly, si-IFN1 and si-IFN2 decreased the expression of VEGF-β. Taken together, serum immunoglobulin fused IFNs increased therapeutic efficacy under current experimental condition.  相似文献   

Impact of feeding method on overall activity of indoor,client‐owned dogs     

D. K. Su  M. Murphy  A. Hand  X. Zhu  A. Witzel‐Rollins 《The Journal of small animal practice》2019,60(7):438-443

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