Identification of QTL controlling high levels of partial resistance to Fusarium solani f. sp. pisi in pea          下载免费PDF全文

Clarice J. Coyne  Marie‐Laure Pilet‐Nayel  Rebecca J. McGee  Lyndon D. Porter  Petr Smýkal  Niklaus J. Grünwald 《Plant Breeding》2015,134(4):446-453

Fusarium root rot is a common biotic restraint on pea yields, and genetic resistance is the most feasible method for improving pea production. This study was conducted to discover quantitative trait loci (QTL) controlling genetic partial resistance to Fusarium root rot caused by Fusarium solani (Mart.) Sacc. f.sp. pisi (F.R. Jones) W.C. Snyder & H.N. Hans (Fsp). A RIL population was screened in a Fusarium root rot field disease nursery for 3 years. Composite interval mapping was employed for QTL detection using the means of disease severity from three growing seasons. Five QTL were identified, including one QTL identified in all three years. The multiyear QTL Fsp‐Ps2.1 contributed to a significant portion of the phenotypic variance (22.1–72.2%), while a second QTL, Fsp‐Ps6.1, contributed 17.3% of the phenotypic variance. The other single growing season QTL are of additional interest as they colocate with previously reported pea–Fusarium root rot resistance QTL. QTL Fsp‐Ps2.1, Fsp‐Ps3.1, Fsp‐4.1 and Fsp‐Ps7.1 are flanked by codominant SSRs and may be useful in marker‐assisted breeding of pea for high levels of partial resistance to Fsp.  相似文献   

SSR Diversity of Vegetable Soybean [<Emphasis Type="Italic">Glycine max</Emphasis> (L.) Merr.]     

Makiko Mimura  Clarice J. Coyne  Marie W. Bambuck  Thomas A. Lumpkin 《Genetic Resources and Crop Evolution》2007,54(3):497-508

Edamame [Glycine max (L.) Merr.] is a type of soybean selected for fresh or frozen vegetable use at an immature stage. Since edamame has a similar protein content, milder flavor, nuttier texture, and is easier to cook when compared to grain soybean, it is being promoted as a new vegetable for global consumption. Global production will require breeding programs for local adaptation; however, limited research has been published on genetic diversity of edamame varieties for the assessment of genetic resources. Simple sequence repeats (SSRs) were used to study the genetic diversity among 130 accessions, including edamame cultivars and landraces from Japan, China and the US, and also the new breeding lines in the US. Although it is assumed that elite edamame cultivars would have narrow genetic diversity, seventeen SSRs detected polymorphism to distinguish 99 of the 130 accessions. The cluster analysis generated nine clusters and 18 outliers. Genetic diversity within Japanese edamame was lower than that within Chinese vegetable soybean accessions (maodou), even though only 10 Chinese maodou were analyzed compared to 107 Japanese edamame. Cluster analysis revealed that the patterns of SSR diversity in edamame can generally distinguish maturity classes and testa color. We concluded that Japanese edamame have a narrow genetic base different from others and that SSRs can describe the patterns of genetic diversity among the elite vegetable soybean.  相似文献   

Evaluating a Rapid On-Farm Nutrient Determination Model for Use in Implementing Nutrient Management Plans     

Stephen F. Higgins  Mark S. Coyne  Scott A. Shearer  John P. Fulton 《Water, air, and soil pollution》2006,171(1-4):333-358

Government regulatory agencies recommend nutrient management plans (NMPs) for animal operations to reduce non-point source pollution. These plans require manure analysis for total nitrogen (TN) and total phosphorus (TP), and use indices to determine nutrient availability. This study evaluated a rapid on-farm method to predict TN and TP concentrations of swine slurries. A field investigation based on this rapid assessment procedure was used to evaluate the effect of a NMP on corn yield and soil fertility. Manure grab samples were collected to validate the rapid on-farm model for predicting TN and TP. A corn crop was raised on two phosphorus (P) soil test levels (medium and excessive) using three randomized complete blocks with two replications of three treatments. Rapid on-farm models were accurate (P ≤ 0.05) for predicting manure TN and TP. The rapid model manure application rate produced grain yields that were significantly higher than inorganic-N fertilization treatments (13,000 kg ha^?1 versus 9,000 kg ha^?1) (P ≤ 0.05). Potassium chloride extractable soil P and ammonium were not significantly different (P ≥ 0.05) in manure treatments compared with the inorganic-N treatment. Analysis of ear leaf N, P, and K and grain yields demonstrated that the rapid model manure application developed by a NMP met crop requirements.  相似文献   

Ethical question of the month — May 2003          下载免费PDF全文

Clarice Lulai 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(8):624

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The precision and robustness of published protocols for disc diffusion assays of antimicrobial agent susceptibility: an inter-laboratory study     

Saoirse Nic Gabhainn   ivind Bergh  Beverly Dixon  Linda Donachie  Jeremy Carson  Rosie Coyne  John Curtin  Inger Dalsgaard  Amedeo Manfrin  Grace Maxwell  Pete Smith 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):1-18

The precision of the disc diffusion protocols previously published by Alderman and Smith (Aquaculture 196 (2002) 211) was analysed in a seven-laboratory trial using Escherichia coli ATCC 25922 as the test strain. Discs containing seven antimicrobial agents were employed and 2899 zone size measurements were generated. The total data generated in the trial was used to quantify the intra- and inter-laboratory precisions. The study design also facilitated the investigation of the influence of the source of media and the source of discs on zone sizes. A smaller two-laboratory trial was employed to investigate the influence of incubation time of zone size.

The intra-laboratory precision was relatively high with the mean of the coefficients of variation calculated for each laboratory and each agent being 4.7%. In contrast, the inter-laboratory precision was very much lower with the mean of the values for each agent being 11.1%. Significant influences on zone size were detected for all three parameters of the protocol. Media source effects were particularly notable with respect to oxytetracycline and oxolinic acid discs, disc source effects with respect to ampicillin and sulphamethoxazole/trimethoprim discs and incubation times with the ampicillin and amoxycillin discs. ANOVA analysis of the total data set confirmed that inter-laboratory variation was the major factor influencing the low precision of the protocol.

The overall precision of the protocols used here was found to be significantly lower than that implied by the control limits associated with the same bacterium in other validated disc diffusion protocols. The implications of these results, for the further development of the protocols under investigation, are discussed.  相似文献   

Immunological characterization of Mycoplasma hyopneumoniae recombinant proteins   总被引：1，自引：0，他引：1  

Simionatto S  Marchioro SB  Galli V  Brum CB  Klein CS  Rebelatto R  Silva EF  Borsuk S  Conceição FR  Dellagostin OA 《Comparative immunology, microbiology and infectious diseases》2012,35(2):209-216

Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, is highly prevalent worldwide and causes major economic losses to the pig industry. Commercial vaccines are widely used in the control of this disease, however, they provide only partial protection. The aim of this study was to evaluate 34 recombinant proteins of M. hyopneumoniae expressed in Escherichia coli. Antigenic and immunogenic properties of these proteins were analyzed. For this, the proteins were tested against hyperimmune and convalescent pig sera through ELISA and Western blot. Immunogenicity of the recombinant proteins was evaluated in BALB/c mice following intramuscular inoculation. Most antigens were able to induce a strong immune response and sera from inoculated mice were able to recognize native proteins by cell ELISA and Western blot. Several recombinant proteins were specifically recognized by convalescent pig sera, indicating they are expressed during infection. These data may help to develop more efficacious vaccines against M. hyopneumoniae.  相似文献   

Extraction, radioiodination, and in vivo catabolism of equine fibrinogen     

C P Coyne  W J Hornof  A B Kelly  T R O'Brien  S J DeNardo 《American journal of veterinary research》1985,46(12):2572-2577

Equine fibrinogen was isolated and aliquots were stored frozen at -70 C before radiolabeling with 125I (half-life = 60.2 days; gamma = 35 keV, using monochloroiodine reagent. Radioiodination efficiencies were 49% to 53%, resulting in a labeled product with 98% protein-bound activity and 91% clottable radioactivity. In 6 equine in vivo investigations, plasma half-lives of 125I-labeled fibrinogen were from 4.1 to 5.2 days, corresponding to a mean daily plasma elimination rate of approximately 15%.  相似文献   

Defense-related gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with non-pathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis     

Pamela Paparu  Thomas Dubois  Danny Coyne  Altus Viljoen   《Physiological and Molecular Plant Pathology》2007,71(4-6):149-157

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Reflex sympathetic dystrophy in a dog.     

A LaBarre  B E Coyne 《Journal of the American Animal Hospital Association》1999,35(3):229-231

Reflex sympathetic dystrophy is a well-recognized syndrome in human patients following injury to an extremity. The syndrome may include hyperesthesia and autonomic changes. The autonomic changes are initial vasodilatation followed by vasoconstriction (e.g., edema followed by cyanosis, and cool skin); hyper- or hypohydrosis; atrophic changes in the skin, subcutis, and muscles; and osteoporosis. Early treatment with a short course of steroids and infiltration of the painful site with lidocaine may alleviate symptoms. If that fails, sympathetic ganglionic block with lidocaine (and possibly steroids) or surgical sympathectomy may provide resolution. A case of reflex sympathetic dystrophy in a dog is presented, involving bilateral distal hind-limb edema and hyperesthesia.  相似文献   

Molecular markers linked to genes for specific rust resistance and indeterminate growth habit in common bean   总被引：2，自引：0，他引：2  

Soon O. Park  Dermot P. Coyne  James M. Bokosi  James R. Steadman 《Euphytica》1999,105(2):133-141

Bulked segregant analysis was utilized to identify random amplified polymorphic DNA (RAPD) markers linked to genes for specific resistance to a rust pathotype and indeterminate growth habit in an F2 population from the common bean cross PC-50 (resistant to rust and determinate growth habit) × Chichara 83-109 (susceptible to rust and indeterminate growth habit). Six RAPD markers were mapped in a coupling phase linkage with the gene ( Ur-9) for specific rust resistance. The linkage group spanned a distance of 41 cM. A RAPD marker OA4.1050 was the most closely linked to the Ur-9 gene at a distance of 8.6 cM. Twenty-eight RAPD markers were mapped in a coupling phase linkage with the gene ( Fin) for indeterminate growth habit. The linkage group spanned a distance of 77 cM. RAPD markers OQ3.450 and OA17.600 were linked to the Fin allele as flanking markers at a distance of 1.2 cM and 3.8 cM, respectively. The RAPD markers linked to the gene for specific rust resistance of Andean origin detected here, along with other independent rust resistance genes from other germplasm, could be utilized to pyramid the different genes into a bean cultivar for durable rust resistance.  相似文献