New dates of a Northern Italian loess deposit (Monte Orfano,Southern pre-Alps,Brescia)     

D’Amico  Michele E.  Casati  Enrico  Andreucci  Stefano  Martini  Marco  Panzeri  Laura  Sechi  Daniele  El Khair  Davide Abu  Previtali  Franco 《Journal of Soils and Sediments》2021,21(2):832-841

Journal of Soils and Sediments - Loess in Northern Italy has been usually considered deposited during the MIS 4-2 period, which corresponds to the last Pleistocene glacial cycle. In particular, no...  相似文献   

Fluorometric sucrose evaluation for sugar beet     

Trebbi D  McGrath JM 《Journal of agricultural and food chemistry》2004,52(23):6862-6867

Sucrose is the economic product from sugar beet. Disease resistance is often available in low-sucrose genotypes and, prior to the deployment of such novel genes as available into the cultivated spectrum, selection for increased sucrose content is required during introgression. The objective of this work was to evaluate a relatively rapid and inexpensive enzymatic-fluorometric microtiter plate assay for sucrose quantification in sugar beet root dry matter, both for progeny testing in the greenhouse and for evaluation of field-grown mother roots. As determined using HPLC, sucrose content in diverse populations of sugar and table beet assayed over various developmental stages ranged from 0.213 to 2.416 mmol g(-1) of dry matter, and these values were used as references for both refractometry and enzymatic-fluorometric assay. As expected, refractometric analysis generally overestimated sucrose content. Enzymatic-fluorometric analyses were reasonably well correlated with HPLC results for young greenhouse-grown root tissues (R2 = 0.976), and less so with older field-grown roots (R2 = 0.605), for unknown reasons. Enzymatic-fluorometric assays may be best deployed for progeny testing of young seedlings.  相似文献   

Distribution of Mercury and Other Heavy Metals in Core Sediments of the Northern Adriatic Sea     

Fabbri  Daniele  Gabbianelli  Giovanni  Locatelli  Clinio  Lubrano  Debora  Trombini  Claudio  Vassura  Ivano 《Water, air, and soil pollution》2001,129(1-4):143-153

Seven sediment cores were collected along a transect about20 km off from the mouth of the Po River, in the northernAdriatic Sea (Italy). Cores were characterised by differentdepositional sequences associated with late Pleistocene-Holocene lowstand (LST), transgressive (TST) and highstand (HST) system tracts. Sediment samples were analysed for mercury, copper, nickel, chromium, manganese and iron, aswell as for total organic matter. Metal distributiongenerally showed vertical and spatial variability ascribed tograin size effects with no significant anthropogenicperturbation. Conversely, mercury showed vertical profilescharacterised by surface enrichment, with concentrations inthe upper layer (50–230 ng g^-1) exceeding 3–11 times the background value of 20 ng g^-1 determined in bottomcores. Surface maxima were attributed to anthropogenic mercurydelivered mainly by the Po River.  相似文献   

Reemergence of Dourine in Italy: Clinical Cases in Some Positive Horses     

Michele Podaliri VulpianiAndrea Carvelli  DVM  Daniele GiansanteFilomena Iannino  DVM  Daniela PaganicoNicola Ferri  DVM 《Journal of Equine Veterinary Science》2013

In 2011, Trypanosoma equiperdum reemerged in Italy, almost 10 years after its last appearance. A total of eight infected horses have been observed to date. Six horses were affected by natural outbreaks of the disease, whereas two were infected experimentally. The aim of this study was to offer a recent perspective on clinical cases of dourine in Europe. Investigation of the clinical aspects confirmed the three stages reported in the literature: stage 1 (genital lesions), stage 2 (cutaneous signs), and stage 3 (nervous signs). The most common signs in the horses under study were notable weight loss, edematous skin eruptions and oedemas of the abdomen, mammary glands and hind legs. Three animals presented neurological signs (lip ptosis of lower lip and ataxia). Infections were paucisymptomatic or asymptomatic in some animals. Hyperthermia was not reported in infected animals and considerable anemia was observed. High antibody titers did not always correspond to clinical signs. Positive polymerase chain reaction test results of blood or tissue (skin, eye swab) often correspond to an advanced stage of the disease. Dourine is a variable disease; owing to its low prevalence and chronic manifestation, it can be difficult to make a quick diagnosis when facing a Dourine-positive horse.  相似文献   

Impacts of changing climate and land use on vegetation dynamics in a Mediterranean ecosystem: insights from paleoecology and dynamic modeling     

Paul D. Henne  Ché Elkin  Daniele Colombaroli  Stéphanie Samartin  Harald Bugmann  Oliver Heiri  Willy Tinner 《Landscape Ecology》2013,28(5):819-833

Forests near the Mediterranean coast have been shaped by millennia of human disturbance. Consequently, ecological studies relying on modern observations or historical records may have difficulty assessing natural vegetation dynamics under current and future climate. We combined a sedimentary pollen record from Lago di Massacciucoli, Tuscany, Italy with simulations from the LandClim dynamic vegetation model to determine what vegetation preceded intense human disturbance, how past changes in vegetation relate to fire and browsing, and the potential of an extinct vegetation type under present climate. We simulated vegetation dynamics near Lago di Massaciucoli for the last 7,000 years using a local chironomid-inferred temperature reconstruction with combinations of three fire regimes (small infrequent, large infrequent, small frequent) and three browsing intensities (no browsing, light browsing, and moderate browsing), and compared model output to pollen data. Simulations with low disturbance support pollen-inferred evidence for a mixed forest dominated by Quercus ilex (a Mediterranean species) and Abies alba (a montane species). Whereas pollen data record the collapse of A. alba after 6000 cal yr bp, simulated populations expanded with declining summer temperatures during the late Holocene. Simulations with increased fire and browsing are consistent with evidence for expansion by deciduous species after A. alba collapsed. According to our combined paleo-environmental and modeling evidence, mixed Q. ilex and A. alba forests remain possible with current climate and limited disturbance, and provide a viable management objective for ecosystems near the Mediterranean coast and in regions that are expected to experience a mediterranean-type climate in the future.  相似文献   

Effect of organic and mineral N fertilizers on N2O emissions from an intensive vegetable rotation     

Daniele De Rosa  David W. Rowlings  Johannes Biala  Clemens Scheer  Bruno Basso  James McGree  Peter R. Grace 《Biology and Fertility of Soils》2016,52(6):895-908

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Antiproliferative terpenoids from almond hulls (Prunus dulcis): identification and structure-activity relationships     

Amico V  Barresi V  Condorelli D  Spatafora C  Tringali C 《Journal of agricultural and food chemistry》2006,54(3):810-814

Bioassay-guided fractionation of the EtOAc crude extract from Sicilian almond hulls, a waste material from Prunus dulcis crop, allowed identification of 10 constituents, isolated as pure compounds (1-5, 7, and 10) or unseparable mixtures (5 + 6 and 8 + 9). All compounds were subjected to spectroscopic analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide bioassay on MCF-7 human breast cancer cells. In addition to the main components oleanolic (1), ursolic (2), and betulinic (3) acids, the 2-hydroxy analogues alphitolic (4), corosolic (5), and maslinic (6) acids, as well as the related aldehydes, namely, betulinic (7), oleanolic (8), and ursolic (9), were identified. From a more polar fraction, the beta-sitosterol 3-O-glucoside (10) was also identified. A sample of commercially available betulin (11) was also included in bioassays as further support to a structure-activity relationship study. Betulinic acid showed antiproliferative activity toward MCF-7 cells (GI50 = 0.27 microM), higher than the anticancer drug 5-fluorouracil.  相似文献   

What is your diagnosis? Torsion of the right middle lung lobe     

Della Santa D  Marchetti V  Lang J  Citi S 《Journal of the American Veterinary Medical Association》2006,229(11):1725-1726

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Evaluating a filtering and recirculating system to reduce dust drift in simulated sowing of dressed seed and abraded dust particle characteristics          下载免费PDF全文

Marcello Biocca  Daniele Pochi  Roberto Fanigliulo  Pietro Gallo  Patrizio Pulcini  Francesca Marcovecchio  Cinzia Perrino 《Pest management science》2017,73(6):1134-1142

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<Emphasis Type="Italic">Asaia</Emphasis> symbionts interfere with infection by Flavescence dorée phytoplasma in leafhoppers     

Elena Gonella  Elena Crotti  Mauro Mandrioli  Daniele Daffonchio  Alberto Alma 《Journal of pest science》2018,91(3):1033-1046

The transmission of microbial pathogens by insect vectors can be affected by the insect’s microbial symbionts, which may compete in colonizing organs, express antagonistic factors or activate host immune response. Acetic acid bacteria of the genus Asaia are symbionts of the leafhopper Scaphoideus titanus, which transmits Flavescence dorée phytoplasma. These bacteria could be used as control agents against the disease. Here, we experimentally investigated the interaction between different strains of Asaia and phytoplasma transmission in the laboratory by using the model leafhopper Euscelidius variegatus and the plant host Vicia faba. We found that uncultivable and low concentrations of Asaia phylotypes were associated with E. variegatus. When we supplied different Asaia strains isolated from other insects and exhibiting different phenotypes to E. variegatus orally, the bacteria stably colonized the leafhopper, reached relatively higher densities and could then be isolated from the host. We conducted transmission trials of Flavescence dorée phytoplasma with individuals colonized with three exogenous Asaia strains. When the phytoplasma became established in the bodies of E. variegatus, leafhoppers were able to transmit it to broad beans, with transmission rates ranging from 33 to 76% in different experiments. However, leafhoppers that were colonized by one of the Asaia strains producing an air–liquid interface biofilm exhibited significantly reduced phytoplasma acquisition, with infection rates at 5–28%, whereas they were 25–77% in control insects. Although the mechanisms regulating this interference remain to be elucidated, our results provide evidence of the potential use of Asaia as a biocontrol agent.  相似文献