湖南省长沙市羊布鲁氏菌病基线调查     

刘增再  谭丹  薛爽  陈智  唐爱明  赖诗嘉  邓钰  宁华杰 《中国动物检疫》2018,35(3):6-9

为了解湖南省长沙市布鲁氏菌病流行现状,对奶羊养殖场(户)和种羊场采用普查策略,对其他羊养殖场(户)采用两阶抽样策略,选择羊群并选定群体中个体,采集血样进行布鲁氏菌抗体检测。同时,通过问卷调查和查阅资料等方式,分析布鲁氏菌病感染和传播风险。结果显示:全市羊布鲁氏菌病表观场群流行率为1.54%(95%CI:0.42%~3.91%),表观个体流行率为0.60%(95%CI:0.43%~0.80%);4个阳性群集中分布于长沙县和宁乡县;和以往监测数据相比,长沙市的羊群布鲁氏菌病流行率和人间发病率均呈下降趋势,说明前期的防治策略取得积极成效。总体来说,长沙市布鲁氏菌病流行率较低,疫情处于稳定控制状态,因而可以分区域逐步实现全市布鲁氏菌病净化。  相似文献   

羊种布鲁氏菌疫苗株PCR-RFLP鉴定方法的建立     

马晓菁  钟旗  叶锋  谷文喜  刘丽娅  马俊杰  薛晶  易新萍 《中国动物检疫》2018,35(7):91-94

为建立一种羊种布鲁氏菌Rev.1疫苗株PCR-RFLP鉴定方法,利用羊种布鲁氏菌Rev.1具有链霉素抗性的特性,选取与链霉素抗性相关编码核糖体蛋白S12的rps L基因为模板设计引物,经PCR扩增后,将产物进行Nci I酶切,发现羊种布鲁氏菌疫苗株Rev.1出现约500 bp条带,而不具有链霉素抗性的羊种布鲁氏菌株16M则出现384 bp条带。结果表明,PCR-RFLP方法能够用于羊种布鲁氏菌Rev.1疫苗株的鉴定,这为今后区分羊种布鲁氏菌疫苗株Rev.1免疫与野株感染提供了基础。  相似文献   

工程造价专业调研思考与启示     

王雪 《吉林农业科技学院学报》2014,(4):118-120

工程造价是在建筑工程管理专业基础上发展起来的新兴学科,由于其就业面广、市场需求量大、薪酬高等优势发展迅速。我校于2014年开设工程造价专科专业,在收集大量资料基础上,针对应用型人才培养模式、＂双师＂型教师培养等问题,确定5所办学效果好、特色鲜明的高职院校进行调研。本文在此基础上,总结了我院开设工程造价专业应思考的问题,并提出了几点建议。  相似文献   

Symbiotic N<Subscript>2</Subscript> fixation and nitrate utilisation in irrigated lucerne (<Emphasis Type="Italic">Medicago sativa</Emphasis>) systems     

Huimin Yang  Murray Unkovich  Ann McNeill  Xianzhi Wang 《Biology and Fertility of Soils》2011,47(4):377-385

Symbiotic N₂ fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18 permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N₂ fixation using ¹⁵N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO₃⁻ by lucerne. On average 65% of lucerne N was obtained from symbiotic N₂ fixation. Converting standing dry matter estimates to annual N₂ fixation amounts we calculated average N₂ fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year. We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher than that of non-N₂ fixing species examined.  相似文献   

拔节期氮肥运筹对不同滴灌量下冬小麦光合特性及产量的影响     

张永强  张璐  陈传信  赛力汗·赛  薛丽华  陈兴武  雷钧杰 《中国农学通报》2020,36(16):1-6

为探明拔节期氮肥运筹对不同滴灌量下冬小麦光合特性及产量的影响,在大田滴灌技术条件下,以‘新冬41号’为试验材料,研究4种拔节期氮肥运筹处理[N0(0 kg/hm²)、N1(90 kg/hm²)、N2(180 kg/hm²)、N3(270 kg/hm²)]对3种不同滴灌量[W1(1500 m³/hm²)、W2(3000 m³/hm²)、W3(3450 m³/hm²)]下冬小麦叶绿素含量（SPAD值）、叶面积指数(LAI)、叶片光合特性及产量的影响。结果表明,在3种滴灌量下各氮肥处理冬小麦拔节至乳熟期叶片SPAD值均呈“先增后降”的变化规律,最大值均在灌浆期;LAI呈现“先增后降”的变化规律,在孕穗期达最大。在同一施氮水平下,滴灌量增加,叶片的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)均增加,而胞间CO₂浓度(Ci)则降低。滴灌量较低时（W1处理）,增加拔节期施氮量能有效提高滴灌冬小麦的有效穗数、穗粒数、千粒重、产量和收获系数;水分充足时（W2、W3处理）,增加拔节期施氮量反而不利于产量的提高。综合考虑各项产量指标及氮肥利用效率得出,在滴灌量3000 m³/hm²条件下,拔节期施氮量180 kg/hm²时,滴灌冬小麦籽粒产量较高,氮肥利用率最大,可供生产参考。  相似文献   

多主体合作博弈视角下林业专业合作社盈余利益分配研究     

马奔  温亚利  薛永基 《绿色中国(A版)》2015,(2):109-113,127

盈余利益分配机制是否合理、完善,对林业专业合作社能否实现成功运转至关重要。文章基于林业专业合作社各利益主体在盈余利益分配上是一个合作博弈的过程,针对传统Shapley值法只考虑贡献率的特点,考虑到林农、投资者、经营者与公司承担风险性大小的差异,利用层次分析法提出改进的Shapely值法,并通过具体算例对改进Shapely值法进行了应用。  相似文献   

Creating high polyploidy pink-flowered strawberries with improved cold tolerance     

Li Xue  Hanping Dai  Jiajun Lei 《Euphytica》2015,206(2):417-426

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E. Melanie DuPuis: Nature’s perfect food: how milk became America’s drink     

Evan Perrault 《Agriculture and Human Values》2011,28(4):583-584

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Books received     

Carol J. Pierce Colfer 《Agriculture and Human Values》2011,28(4):589-590

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Performance evaluation of forest co-management: a case study of Chunati Wildlife Sanctuary,Bangladesh     

Umma Tamima 《林业研究》2016,27(4):853-861

The objective was to evaluate the performance of the co-management of Nishorgo Support Project at Chunati Wildlife Sanctuary in Bangladesh. I adopted the Focus Groups Discussion method for opinion survey and applied the SWOTAHP technique for data analysis. Local people did not participate in the decision-making process of forest management and they perceived co-management as a threat to their livelihoods. Moreover, mistrust and misunderstanding among different stakeholders, political and ethical conflicts, lack of property rights of tribal communities, and lack of accountability and transparency were identified as the drawbacks of co-management.  相似文献