Effects of a Dual‐Frequency Frequency‐Sweeping Ultrasound Treatment on the Properties and Structure of the Zein Protein     

Xiaofeng Ren  Xun Wei  Haile Ma  Huiji Zhou  Jingjing Guo  Shuyun Mao  An Hu 《Cereal Chemistry》2015,92(2):193-197

We investigated the effects of a dual‐frequency frequency‐sweeping ultrasound (DFFSU) treatment on the functional properties and structure of zein. The solubility of ultrasound‐treated zein proteins increased slightly but significantly as the treatment time increased. The results showed that the DFFSU treatment had an obvious influence on the mean particle size and the size distribution. A significant (P < 0.05) increase in the size of the particles with respect to time was observed after a sonication time of more than 20 min in zein solutions. Differential scanning calorimetry results showed that sonication alters the thermal behavior of zein. Circular dichroism spectra showed a small increase in the percentage of ordered structure elements within the protein molecule. After 60 min of ultrasonication, the percentage of α‐helix structures increased by 0.9%, whereas the percentage of β‐sheets and β‐turns decreased by 0.5%. Microstructural analyses by scanning electron microscopy showed that several microholes appeared in the zein following ultrasonic pretreatment. Under the conditions investigated in this study, DFFSU treatment was found to affect the studied functional properties of the zein protein. This technology could be used to obtain improved functional properties in some protein samples.  相似文献   

手持式电动绿篱修剪机的研究与设计   总被引：1，自引：0，他引：1  

杨天兴  赵春花  安虎平  柴昌盛 《中国农机化学报》2015,(3):67-69,73

通过对目前市场上绿篱修剪机结构和使用特点的详细分析,设计了一种用220V照明电为电源、手提砂轮机作为主机,用专门制作的切割器替换砂轮片实现快速修剪成形的手持式电动绿篱修剪机。试验表明,该修剪机有很好的实用价值和推广前景。  相似文献   

台阶式溢洪道消能规律     

杨吉健  刘韩生  张峰  文明宜  安梦雄 《排灌机械工程学报》2015,(2):123-127

为探讨台阶式溢洪道消能特性及水流的紊乱特性规律,将台阶式溢洪道与对应光滑溢洪道对比研究,通过试验数据计算出台阶式溢洪道消能率、阶顶断面弗劳德数,试算求出对应光滑溢洪道水力参数,用台阶式溢洪道消能率减去对应光滑溢洪道消能率得到相对消能率,光滑溢洪道断面弗劳德数减去对应台阶式溢洪道阶顶断面弗劳德数得到相对弗劳德数.两水力参数反映了台阶式溢洪道相对光滑溢洪道对消杀能量大小及水流紊乱剧烈程度.通过对26.57°,32.01°,33.69°,38.66°,51.3°这5组不同坡度,1.43~6.67 cm台阶高度的21组模型试验研究,探讨了相对消能率和相对弗劳德数之间关系,结果表明相对弗劳德数和相对消能率沿程呈线性关系,相关系数为0.993 7~0.999 5.且相同相对弗劳德数,单宽流量大对应相对消能率也大;台阶高度变化对直线关系影响很小;坡度对直线关系影响复杂,较小坡度同一单宽流量,坡度越大相对消能率越小,坡度较大时同一单宽流量,坡度大对应相对消能率大.相对消能率和相对弗劳德数的直线规律体现出台阶式溢洪道独特的水力特性,为探究其消能机理提供了重要依据.  相似文献   

供油提前角对甲醇发动机性能及排放的影响     

高聪慧  朱建军  王晋  安俏俏 《中国农机化》2015,(3):233-237

利用在缸内加装电热塞的ZS1115单缸柴油,实现纯甲醇(M100)燃料的扩散燃烧,利用柴油机热效率高的优点,提高甲醇燃料的能量转换效率。通过改变供油提前角,研究不同供油提前角对甲醇发动机性能及排放的影响,并与原单缸柴油机燃用0#柴油进行对比。试验结果表明:供油提前角对甲醇发动机动力性、经济性及排放均有影响。与原机燃用0#柴油相比,燃油消耗率上升,使用成本降低;HC排放量升高,CO和NOX排放量降低。供油提前角提前4°CA时CO排放最少,最低平均减少23.89%;供油提前角退后2°CA时NOX排放最少,最低平均减少94.74%。  相似文献   

青核桃去皮过程的受力分析     

郑甲红  吴东泽  梁金生  牛硕雅  安碾粮 《农机化研究》2015,(8):153-156

青核桃在去皮方面不断进取的同时,存在着共同的问题是去掉的青皮被打成泥状,对核桃和环境都造成再次污染。为此,将通过模拟青核桃去皮过程的受力进行试验,分别从青核桃的尺寸、加载方向及加载速度等可能影响青核桃去皮过程的因素进行分析研究,为使青皮脱掉后不会被打成泥状,而是呈块状脱出奠定实验基础。  相似文献   

Canine fetal echocardiography: correlations for the analysis of cardiac dimensions     

Amália Turner Giannico  Elaine Mayumi Ueno Gil  Daniela Aparecida Ayres Garcia  Marlos Gonçalves Sousa  Tilde Rodrigues Froes 《Veterinary research communications》2016,40(1):11-19

The aim of this study was to develop regression models for correlation of canine fetal heart development with body size to characterize normal development or suggest cardiac anomalies. Twenty clinically healthy pregnant bitches, either brachycephalic and non-brachycephalic, were examined ultrasonographically. Transabdominal fetal echocardiography was conducted every 4 days from the beginning of cardiac chambers differentiation until parturition. Ten cardiac parameters were measured: length, width and diameter of the heart; heart area; left and right ventricular dimensions; left and right atrial dimensions; and aortic and pulmonary artery diameter. Femoral length, biparietal diameter and abdominal cross-sectional area were also recorded. Regression equations were developed for each parameter of fetal body size, and linear and logarithmic models were compared. The model with the highest correlation coefficient was chosen to produce equations to calculate relative dimensions based on the correlations. Only the left-ventricular chamber differed between the two racial groups. Biparietal diameter was the independent parameter that produced the highest correlation coefficient for the most fetal cardiac dimensions, although good correlations were also observed using femoral length and abdominal cross-sectional area. Heart width and heart diameter were used as surrogates of cardiac development, as these measurements showed the best statistical correlation. Quantitative evaluation of fetal cardiac structures can be used to monitor normal and abnormal cardiac development.  相似文献   

Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology     

Chiara Gomiero  Giulia Bertolutti  Tiziana Martinello  Nathalie Van Bruaene  Sarah Y. Broeckx  Marco Patruno  Jan H. Spaas 《Veterinary research communications》2016,40(1):39-48

Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.  相似文献   

Detection of novel polymorphisms in the ckit gene of canine patients with lymphoma,melanoma, haemangiosarcoma,and osteosarcoma     

Irina Gramer  Martin Kessler  Joachim Geyer 《Veterinary research communications》2016,40(2):89-95

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Immunological and pathological investigations in equine experimental uveitis     

Galina P. Simeonova  Svetozar Z. Krastev  Radostin S. Simeonov 《Veterinary research communications》2016,40(3-4):107-115

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Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD     

王园  王文文  杨毅  高畅  郭焘  段婷  刘娜  安晓萍  齐景伟 《动物营养学报》2023,(1):380-390

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.  相似文献