应用昆虫病原线虫防治杨木蠹蛾的研究   总被引：1，自引：0，他引：1  

潘洪玉  丁利  胡汉桥  张秀荣  杨军  刘波  刘占东  李兵  张希堂 《昆虫天敌》1995,(2)

室内外试验结果表明,小卷蛾线虫Steinernema carpocapsae Agriotos对杨木囊蛾Cossus cossusL.的致病力较强,其毒力随着龄期的增大而下降。从每虫1000～5000条线虫接种中龄组的杨木蠢蛾幼虫,随着剂量增加致病力增强,并在25℃条件下毒杀效果最好。以每毫升5000条线虫的浓度防治林间杨木蠹蛾,防治效果优于敌敌畏。  相似文献   

Chinese Academy of Sciences. In China, publish or perish is becoming the new reality     

Ding Y 《Science (New York, N.Y.)》2001,291(5508):1477-1479

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昆虫生长调节剂和生物农药防治韭蛆田间药效试验   总被引：6，自引：0，他引：6       下载免费PDF全文

陈栋  张思聪  张龙 《植物保护》2005,31(1):82-84

对3种昆虫生长调节剂和2种生物农药对韭蛆田间防效进行了初步评价。春季试验结果表明,10%高渗烟碱水剂1000倍液防效最好,达70.0%以上;70%灭蝇胺可湿性粉剂3000倍液和0.3%印楝素乳油1000倍液次之;而5%卡死克乳油800倍液和5%抑太保乳油1000倍液的防效较差。将春季试验药效较好的3种农药用于秋季田间试验,结果表明,10%高渗烟碱水剂1000倍液和70%灭蝇胺可湿性粉剂3000倍液防效较好,特别是10%高渗烟碱水剂的防效达到79.8%,几乎与40%辛硫磷乳油1000倍液相当。0.3%印楝素乳油1000倍液的防效较差,仅为69.2%。  相似文献   

大豆制品转基因成分检测和定量标识分析     

董流昕  谢响明  毛建军  陈红运  陈洪俊  朱水芳 《植物检疫》2005,19(3):132-134

分别以大豆凝集素(Lectin)和5-莽草酸-3磷酸合成酶(CP4-EPSPS)为内源和目标基因,对28种大豆制品进行实时荧光PCR检测.检测结果表明,4种大豆制品的CP4-EPSPS扩增结果为阳性,含有转基因大豆成分.转基因成分的定量检测在贸易和可操作性方面要优于定性检测.  相似文献   

西花蓟马的生物学习性观察试验   总被引：6，自引：1，他引：6  

刘忠善  杨小溪  丁元明 《植物检疫》2005,19(3):138-141

在温室内以粉色玫瑰为寄主植物观察并记录了西花蓟马的部分生物学习性,在恒温箱内记录了在15℃、20℃、25℃、30℃4个温度下各虫态的历期,并计算出发育起点温度和有效积温.  相似文献   

Cell cycle arrest induced by hypoxia inducible factor-1 alpha in SW626 cell line of human ovarian cancer     

HUANG Lei  AO Qi-lin  LI Fang  XING Hui  LU Yun-ping  LIAO Guo-ning  MA Ding 《园艺学报》2005,21(4):718-721

AIM: To investigate the cell cycle arrest induced by hypoxia, hypoxia inducible factor-1 and their possible mechanism in human ovarian cancer cell line SW626. METHODS: CoCl2, a chemical inducer of hypoxia and hypoxic cell culture chamber were used to induce chemical and physical hypoxia in human ovarian cancer cell line SW626. The method of ‘decoy’ was used to block the function of HIF-1α because it acts as the core sequence of the target gene as a competitor combined to the HIF-1α. The cells were divided into group A1 (normal oxygen), A2 (normal oxygen plus HIF-1α decoy), B1 (CoCl2), B2 (CoCl2 plus HIF-1α decoy), C1 (hypoxia) and C2 (hypoxia plus HIF-1α). The expression of the HIF-1α protein, mRNA and cell cycle analysis were detected by Western blotting, RT-PCR and flow cytometry (FCM). RESULTS: The expression level of HIF-1α protein in group B1 (3.75±1.31) and group C1 (3.48±1.01) was significantly higher than that in group A1 (0.97±0.31) (P<0.05). The expression levels of HIF-1α mRNA in group A1 (0.65±0.32) and group B1 (0.64±0.34) were significantly lower than that in group C1 (1.28±0.62) (P<0.05). Decoy had no effect in the expression of HIF-1α protein and mRNA level (P>0.05). FCM showed that the G0/G1 phase was markedly increased in group B1 (81.78±24.33) and group C1 (77.62±22.76) and was significantly higher than that in group A1 (49.49±18.54) (P<0.05), group B2 (61.54±20.84) was lower than that in group B1 with statistical significance (P<0.05) and group C2 (56.03±21.42) was lower than that in group C1 with statistical significance (P<0.05) , but the difference between group A1 and group A2 (51.77±16.45) had no statistical significance (P>0.05). CONCLUSION: Both CoCl2 and physical hypoxia could distinctly induce cell cycle arrest in G0/G1 phase and the expression of HIF-1α in human ovarian cancer cell line SW626. HIF-1α plays an important role in cell cycle arrest induced by hypoxia in human ovarian cancer cell line SW626.  相似文献   

钴在奶牛营养中的研究与应用     

董小英  唐胜球  戚融冰 《饲料广角》2005,(9):23-24

钴是奶牛营养中不可缺乏的微量元素之一,在奶牛机体内的新陈代谢、生长发育、繁殖以及免疫等方面发挥重要作用。笔者主要就钴在动物机体内的新陈代谢、生物学功能及其在奶牛营养中的应用等方面进行了综述。  相似文献   

Extracts of killedPenicillium chrysogenum Induce resistance against Fusarium wilt of melon     

Hezhong Dong  Yigal Cohen 《Phytoparasitica》2001,29(5):421-430

Dry fungal biomass ofPenicillium chrysogenum (dry mycelium), a waste product of the pharmaceutical industry, was extracted with water and applied to the roots of melon plants before or after inoculation withFusarium oxysporum f.sp.melonis (Font). Seedlings (4–6 days after emergence) treated with either acidic dry mycelium extract (DME) or neutralized dry mycelium extract (NDME) were protected against challenge infection withFom. A single drench with 2–5% DME applied 12–72 h before inoculation provided significant control of the disease compared with water-drenched, challenged seedlings. No protection was seen in plants treated 0–6 h before inoculation or 0–48 h after inoculation. Neither DME nor NDME (0.5–5%) had any effect on fungal growthin vitro, which implied that disease controlin vivo was mediated by induced resistance. The resistance induced by DME protected melon plants not only against race 1,2, but also against the three other races of the pathogen, indicating a race-non-specific resistance againstFom. Both DME and NDME significantly increased peroxidase activity and free L-proline content in seedlings 12 h and 48 h after soil drench, respectively. Resistance to Fusarium wilt was significantly associated with elevated levels of peroxidase activity but not with free L-proline content. Thus, peroxidase might be involved in the defense mechanisms activated by DME or NDME. http://www.phytoparasitica.org posting Aug. 31, 2001.  相似文献   

陕西省果树黑星病发生现状及药剂防治策略     

时春喜  董雪娟  陈志军  屈爱荣 《农药科学与管理》2001,(Z1)

苹果、梨黑星病的初侵染发生率高低和发病中心的形成与当年5～6月份降雨量密切相关 ,而流行则与7～8月份降雨量密切相关 ,在防治策略上应重点抓住5～6月份的预报工作 ,药剂施用上应选择保护性杀菌剂及内吸性杀菌剂交替使用 ,在发病初期每隔7d喷药1次 ,连续喷3～4次 ,防效可达80%～85%。  相似文献   

调整玉米播期对玉米粗缩病控制作用研究初报   总被引：1，自引：0，他引：1  

丁志宽  钱爱林  姜海洲  梅爱中 《江西植保》2001,24(2):33-36

通过罩笼接虫和自然开放式状态下试验 ,对不同播期的玉米粗缩病病情进行了比较 ,明确了玉米敏感叶龄为可见叶 9叶以下 ,展开叶为 6叶以下。研究证实 ,将田间灰飞虱转移迁入高峰期与玉米敏感叶龄期错开 ,可以减轻玉米粗缩病的发病 ,春玉米播期 4月 10～ 15日 ,夏玉米在 6月 10～ 15日 ,能显著控制玉米粗缩病的危害。  相似文献