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71.
Arai T Nakamura M Magori E Fukuda H Mizutani H Kawakami E Sako T 《Research in veterinary science》2002,73(2):183-186
Changes in plasma glucose, immunoreactive insulin (IRI), and free fatty acids (FFA) concentrations and activities of enzymes related to energy metabolism in peripheral leukocytes were measured in type 1 diabetic dogs induced by streptozotocin administration before and after intensive insulin treatments. Before the insulin treatment, the plasma glucose concentrations were over 16.7 mmol/L and the activities of enzymes remarkably decreased in leukocytes of the diabetic dogs. In particular, malate dehydrogenase (MDH) activities decreased significantly. After glycemic control was achieved by insulin treatment, the plasma glucose concentrations were maintained between 6.8 and 7.7 mmol/L and the activities of enzymes restored to those in the control dogs. The changes in ML ratio (cytosolic MDH/lactate dehydrogenase specific activity ratio) reflected faithfully to metabolic conditions of animals and were considered to be a useful parameter to evaluate glycemic control and the effect of injected insulin in the diabetic dogs. 相似文献
72.
Comparison of time-action profiles of insulin Glargine and NPH insulin in normal and diabetic dogs 总被引:2,自引:1,他引:1
Mori A Sako T Lee P Motoike T Iwase K Kanaya Y Fukuta H Mizutani H Arai T 《Veterinary research communications》2008,32(7):563-573
Intermediate insulin injections are commonly used for glycemic control in insulin dependent diabetic dogs acting as a replacement
for natural insulin. Neutral Protamin Hagedorn (NPH) insulin and insulin glargine are two types of injectable insulin preparations
commonly used in humans. In our study, we investigated the time-action profiles of both aforementioned insulin preparations
in normal dogs in order to determine whether co-administration of NPH and glargine would be of benefit to insulin dependent
diabetic dogs as it is for humans suffering from insulin dependent diabetes. Time-action profiles of NPH insulin and insulin
glargine in normal dogs demonstrated a clear difference between both insulin preparations confirming that NPH insulin is an
intermediate-acting preparation whereas insulin glargine is a long-lasting preparation. In addition, co-administration of
NPH insulin and insulin glargine resulted in tight glycemic control as compared to NPH insulin alone in insulin dependent
diabetic dogs. However, co-administration result in hypoglycemia at the dosages tested. 相似文献
73.
Toyoda Y Sako T Mizutani H Sugiyama M Hayakawa N Hasegawa H Hirose H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1091-1093
When a solution of xylitol was rapidly administered intravenously (bolus infusion) to healthy cattle or those with ketosis, different results were obtained. In healthy cattle, a temporary surge in insulin secretion was observed, whereas in ketotic cattle no such surge was found, but instead a moderate level of secretion continued for a lengthy period. No significant difference in the areas under the insulin curve (AUC) was found between healthy cattle and ketotic cattle up to 120 min after xylitol infusion. These results clearly demonstrated that a bolus infusion of xylitol solution in ketotic cattle does not cause a temporary surge in insulin secretion unlike in healthy animals, but rather results in a continuous, gradual rise in secretion. 相似文献
74.
Measurement of transepidermal water loss (TEWL) in cats with experimental skin barrier dysfunction using a closed chamber system
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75.
76.
We report a new strain of Melon necrotic spot virus (MNSV) that is unable to systemically infect Cucumis melo. A spherical virus (W-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. The W-isolate had little serological similarity to MNSV, and it did not cause any symptoms in six melon cultivars susceptible to MNSV; however, the host range of the W-isolate was limited exclusively to cucurbitaceous plants, and transmission by O. bornovanus was confirmed. Its genomic structure was identical to that of MNSV, and its p89 protein and coat protein (CP) showed 81.6 to 83.2% and 74.1 to 75.1% identity to those of MNSV, respectively. Analysis of protoplast showed that the W-isolate replicated in melons at the single-cell level. Furthermore, chimeric clones carrying the CP of MNSV induced necrotic spots in melons. These results suggested that the absence of symptoms in melons was due to a lack of ability of the W-isolate to move from cell to cell. In view of these findings, we propose that the new isolate should be classified as a novel MNSV watermelon strain. 相似文献
77.
Y. Hatano N. Mori M. Asada A. Mori I. Yamamoto S. Muranaka M. Kojima M. Kigure M. Yagishita T. Sako T. Arai 《Research in veterinary science》2010,88(3):458-460
Metabolite, insulin and adiponectin concentrations and LDH, AST and ALT activities were measured in plasma of 142 client-owned cats (1–13 years old, 16 breeds) to set up a new criterion of hypertriglyceridemia (hyper-TG) with increased plasma insulin concentrations for early diagnosis of lipid metabolism abnormality including obesity. 25 cats with over 165 mg/dl of plasma triglyceride (TG) concentrations were decided as hyper-TG with increased plasma insulin concentrations, and prevalence of hyper-TG was 16.7% in young (1–6 years old) and 18.3% in old (>7 years old) cats examined. In the hyper-TG cats, their plasma TG concentrations increased to 6.6–7.4-fold of the values of control cats with 35–50 mg/dl of plasma TG and their plasma cholesterol, FFA and insulin concentrations and LDH and ALT activities increased significantly, whereas their plasma adiponectin concentrations decreased significantly compared to those in the control cats. Hyper-TG cats with significantly increased body weights and plasma insulin and decreased plasma adiponectin seemed to be in early stage of obesity accompanying increased plasma insulin concentrations. Increased TG, insulin, LDH and ALT and decreased adiponectin values in plasma seemed to be key factors for diagnosis of lipid metabolism abnormality at early stage in cats. 相似文献
78.
79.
Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk‐3)
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K. Ochiai H. Oda S. Shono Y. Kato S. Sugihara S. Nakazawa D. Azakami M. Michishita E. Onozawa M. Bonkobara T. Sako L. Shun‐Ai H. Ueki M. Watanabe T. Omi 《Veterinary and comparative oncology》2017,15(4):1181-1186
Reduced expression in immortalized cells (REIC/Dkk‐3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk‐3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk‐3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk‐3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk‐3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk‐3 represents a potential molecular target for the development of therapies against feline mammary cancers. 相似文献
80.
Haile Desmae Pasupuleti Janila Patrick Okori Manish K. Pandey Babu N. Motagi Emmanuel Monyo Omari Mponda David Okello Dramane Sako Candidus Echeckwu Richard Oteng‐Frimpong Amos Miningou Chris Ojiewo Rajeev K. Varshney 《Plant Breeding》2019,138(4):425-444
Groundnut is an important food and oil crop in the semiarid tropics, contributing to household food consumption and cash income. In Asia and Africa, yields are low attributed to various production constraints. This review paper highlights advances in genetics, genomics and breeding to improve the productivity of groundnut. Genetic studies concerning inheritance, genetic variability and heritability, combining ability and trait correlations have provided a better understanding of the crop's genetics to develop appropriate breeding strategies for target traits. Several improved lines and sources of variability have been identified or developed for various economically important traits through conventional breeding. Significant advances have also been made in groundnut genomics including genome sequencing, marker development and genetic and trait mapping. These advances have led to a better understanding of the groundnut genome, discovery of genes/variants for traits of interest and integration of marker‐assisted breeding for selected traits. The integration of genomic tools into the breeding process accompanied with increased precision of yield trialing and phenotyping will increase the efficiency and enhance the genetic gain for release of improved groundnut varieties. 相似文献