Different levels of selenomethionine on the meat quality and selenium deposition in tissue of finishing pigs     

Vanessa Avelar Silva  Antnio Gilberto Bertechini  Alisson Hlio Sampaio Clemente  Luis Filipe Villas Boas de Freitas  Bernardo Rocha Franco Nogueira  Barbara Lopes de Oliveira  Alcinia de Lemos Souza Ramos 《Journal of animal physiology and animal nutrition》2019,103(6):1866-1874

The aim of the study was to verify the influence of selenomethionine (SM) supplementation on performance, carcass yield, characteristics of meat quality and Se tissue deposition of finishing pigs. A total of 128 hybrid pigs with an average weight of 76 kg were distributed in randomized blocks according to body weight in eight treatments and eight replicates. The experimental treatments were two Se levels from sodium selenite‐SS (0.3 and 0.6 ppm), four Se levels from SM (0.3, 0.4, 0.5 and 0.6 ppm) and two combinations of SS with SM (SS 0.15 + SM 0.15 ppm and SS 0.3 + SM 0.3 ppm) providing 0.3 and 0.6 ppm Se in the diet respectively. The feeds were based on corn and soya bean meal. After 30 days on test, were analysed the performance indices and the pigs were slaughtered at commercial slaughterhouse. The cold carcass yield, the physicochemical characteristics of the loin meat and the Se content in muscle and liver were evaluated. There was no significant difference in performance indices (p > .05); however, there was a linear effect on the increase in pig carcass yield by increasing SM (p < .05). The use of SM solely or combined with SS provided higher Se deposition in muscle compared to SS (p < .05). The highest Se deposition in muscle occurred for SM at 0.4 ppm (p < .05). The SS provided higher Se deposition in liver (p < .05). The SM presented best results for meat quality compared to other sources (p < .05). The level of 0.4 ppm Se promoted the best results for the indices of yellow, luminosity, cooking loss and pH (p < .05). The use of SM at any level promotes higher oxidation stability of pig meat (p < .05). The supplementation of SM at a level of 0.4 ppm promotes better physicochemical characteristics and higher Se deposition on swine meat.  相似文献   

Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation,mitochondrial activity and glutathione levels     

Tiago S. Nascimento  Isabelle S. M. Silva  Maria Cecília M. A. Alves  Bruna B. Gouveia  Lara Mariane R. Barbosa  Taís J. S. Macedo  Jamile M. S. Santos  Alane P. O. Monte  Maria Helena T. Matos  Francine F. Padilha  Isabel B. Lima‐Verde 《Reproduction in domestic animals》2019,54(1):31-38

This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high‐performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml—HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species—ROS and glutathione—GSH) and active mitochondria. HPLC showed formononetin as the major compound in the HERP (63.92 ± 0.21 μg/mL). The content of flavonoids ranged from 2.14% to 11.00%. The nanoparticles showed mean diameter of 287.5 ± 3.9 and 479 ± 18.1 ηm; PI of 0.117 ± 0.018 and 0.316 ± 0.039; and EE of 67.15% and 41%, respectively, for the NP10 and NP20 groups. After 12 days of culture, HERP20 and NP20 increased (p < 0.05) the percentage of normal follicles compared to NP10. HERP20 showed significantly higher percentages of antrum formation than control medium (MEM) and NP10 (p < 0.05). HERP20 also showed an increase (p < 0.05) in mitochondrial activity compared to the other treatments, except NP20 (p > 0.05), and increased GSH levels (p < 0.05) compared to MEM and HERP10. In conclusion, use of HERP (20 ηg/ml) on in vitro culture of isolated ovine preantral follicles can increase antrum formation, mitochondrial activity and GSH levels.  相似文献   

Herd characteristics and management practices associated with bulk tank milk quality of dairy herds in southeastern Brazil     

Cortinhas  Cristina Simões  Botaro  Bruno Garcia  de Macedo  Susana Nori  dos Santos  Marcos Veiga 《Tropical animal health and production》2018,50(7):1605-1610

Tropical Animal Health and Production - This study identified the association of management practices and herd characteristics with milk quality of bulk tanks in southeastern, Brazil. Milk samples...  相似文献   

Low occurrence of pathogenic Yersinia enterocolitica in pig tonsils at slaughter in Southern Brazil     

Wildemann  Paula  Gava  Danielle  Sfaciotte  Ricardo Antônio Pilegi  Melo  Fernanda Danielle  Ferraz  Sandra Maria  da Costa  Ubirajara Maciel  Vaz  Eliana Knackfuss 《Tropical animal health and production》2018,50(3):671-675

Tropical Animal Health and Production - Yersinia enterocolitica is a foodborne pathogen and pigs are the main reservoir of it in their tonsils. As Brazil is a large producer and exporter of pork...  相似文献   

Increasing levels of supplementation for crossbred steers on pasture during the dry period of the year     

Daniele Soares Barroso Neves  Robério Rodrigues Silva  Fabiano Ferreira da Silva  Laize Vieira Santos  George Abreu Filho  Sinvaldo Oliveira de Souza  Marceliana da C. Santos  Weder Jansen Rocha  Ana Paula Gomes da Silva  Mateus de Melo Lisboa  Maria Magna Silva Pereira  Venício Macedo Carvalho 《Tropical animal health and production》2018,50(7):1411-1416

The aim of this study was to evaluate the effect of increasing concentrate supplementation levels on the intake, nutrient digestibility, and performance of crossbred steers during the dry period of the year. The experiment was developed on Princesa do Mateiro farm, in the municipality of Ribeirão do Largo, located in the southwest region of Bahia State, Brazil. Forty uncastrated male crossbred (½ Holstein-Zebu) steers with an average body weight (BW) of 232.55?±?24.97 kg were distributed into four treatments in a completely randomized design with ten replicates. The animals were managed in an experimental area formed by Brachiaria brizantha cv. Marandu, in an intermittent grazing system. Treatments consisted of the following supplementation levels: 0.2% BW, with 60% crude protein (CP); 0.3% BW, with 40% CP; 0.4% BW, with 30% CP; and 0.5% BW, with 24% CP. The intakes of forage dry matter in kg/day and %BW and neutral detergent fiber corrected for ash and protein (NDFap) in %BW decreased linearly, whereas the intake of non-fibrous carbohydrates corrected for ash and protein in kg/day and average daily gain increased linearly. Therefore, the use of supplementation at 0.5% BW (24% crude protein) to provide gains of up to 0.500 kg/day is recommended for grazing steers during the post-weaning period in the dry season of the year.  相似文献   

Feeding of juvenile cobia,Rachycentron canadum: Evaluation of practical feeds,comparison of commercial fishmeal replacers,and estimation of essential amino acid requirements     

Thiago Raggi  Albert G. J. Tacon  Daniel Lemos 《Journal of the World Aquaculture Society》2019,50(2):317-335

Three studies were conducted with juvenile cobia, Rachycentron canadum: (Study 1) a 10‐week feeding trial within floating net cages to test the nutritional efficacy of different dietary feeding regimes (trash‐fish control diet, a semimoist diet, an in‐house dry formulated diet, and a commercial cobia feed); (Study 2) a 10‐week feeding trial within an indoor water‐recirculating tank‐based system to test the nutritional efficiency of different potential dietary fishmeal replacers (poultry byproduct meal, soy protein concentrate, feather meal), a diet without taurine supplementation, and a commercial cobia feed; and (Study 3) estimation of the essential amino acid (EAA) requirements of cobia based on EAA whole‐body composition in fast‐growing cobia fed a trash‐fish‐based diet. Fish performance in terms of growth and feed efficiency was the greatest within the outdoor net‐cage feeding trial, with fish fed the control trash‐fish‐based diet exhibiting the best performance. Although fish growth was poorer within the indoor feeding trials, fish performance was similar for most diets, with apparent crude protein digestibility coefficients of over 75% being obtained in all experimental diets in both feeding trials. The estimated EAA requirements of cobia obtained during this study were similar to those reported for other similar marine carnivorous fish species.  相似文献   

Damage by Homalinotus depressus in commercial coconut palm crops in the Amazonian region of Brazil     

Rafael Coelho Ribeiro  Walkymário de Paulo Lemos  Paulo Manoel Pontes Lins  Roberta de Melo Valente  Antonio Agostinho Müller  Alexandre Mehl Lunz  José Cola Zanuncio 《Phytoparasitica》2012,40(3):239-241

The coconut palm (Cocos nucifera L., Arecaceae) has great economic and social importance for many industrial and non-industrial products. Pests can reduce its productivity and cause its death. In 2005, larvae and adults of a Curculionidae were observed damaging the floral stalk of coconut palms in commercial plantations in the municipality of Moju, Pará State, in the Brazilian Amazon. Insects were identified as the black coconut bunch weevil, Homalinotus depressus (L.) (Coleoptera: Curculionidae: Cholini). This is the first report of this pest damaging coconut palms in Brazil.  相似文献   

Antibacterial activity of the essential oil from Lippia aff. gracillis     

Pessoa OD  de Carvalho CB  Silvestre JO  Lima MC  Neto RM  Matos FJ  Lemos TL 《Fitoterapia》2005,76(7-8):712-714

The essential oil of fresh leaves of Lippia aff. gracillis was analyzed by GC/MS and evaluated for its antibacterial effects. The results showed a moderate antibacterial activity and confirm the traditional uses of L. aff. gracillis.  相似文献   

Comparison of Analgesic Effects of Caudal Epidural 0.25% Bupivacaine with Bupivacaine Plus Morphine or Bupivacaine Plus Ketamine for Analgesia in Conscious Horses     

Rafael DeRossi  Tiago J.C. MódoloRonaldo C. Pagliosa  DVM  Paulo H.A. JardimFelipe B. Maciel  DVM  Gustavo G. Macedo  DVM  MSc  Dr 《Journal of Equine Veterinary Science》2012

The aim of this study was to compare the effects of caudal epidural bupivacaine alone (BP), bupivacaine plus morphine (BPMP), and bupivacaine plus ketamine (BPKE) for perineal analgesia in horses. Each of the six saddle horses received a caudal epidural catheter and underwent 3 treatments: BP, 0.25% (0.04 mg/kg) bupivacaine hydrochloride without epinephrine; BPMP, 0.02 mg/kg of bupivacaine combined with 0.1 mg/kg of morphine-preservative free; and BPKE, 0.02 mg/kg of bupivacaine combined with 0.5 mg/kg of ketamine. The order of treatments was randomized. The cardiovascular system, respiratory rate, quality of analgesia, sedation, and motor blockade were assessed before drug administration (baseline), at 5, 10, 15, and 30 minutes, and every 30 minutes thereafter until loss of analgesia. The median time to onset of analgesia was 5 minutes after BP treatment, faster than after BPKE or BPMP treatments, which were 10 minutes and 15 minutes, respectively (P < .05). The BPMP treatment produced analgesia (315 minutes) for a longer duration than BP treatment (210 minutes) or BPKE treatment (240 minutes), in the regions of the tail, perineum, and upper hind limb in horses. All treatments presented mild sedation or motor blockade. There were minimal effects on the cardiovascular system and respiratory rate. BPMP may be preferable to a high dose of BP or BPKE. Caudal epidural BPMP can be an appropriate choice for regional perineal analgesia in horses.  相似文献   

Application of a multiplex PCR assay for Campylobacter fetus detection and subspecies differentiation in uncultured samples of aborted bovine fetuses     

Gregorio Iraola  Martín Hernández  Lucía Calleros  Fernando Paolicchi  Silvia Silveyra  Alejandra Velilla  Luis Carretto  Eliana Rodríguez  Ruben Pérez 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):371-376

Campylobacter (C.) fetus (epsilonproteobacteria) is an important veterinary pathogen. This species is currently divided into C. fetus subspecies (subsp.) fetus (Cff) and C. fetus subsp. venerealis (Cfv). Cfv is the causative agent of bovine genital Campylobacteriosis, an infectious disease that leads to severe reproductive problems in cattle worldwide. Cff is a more general pathogen that causes reproductive problems mainly in sheep although cattle can also be affected. Here we describe a multiplex PCR method to detect C. fetus and differentiate between subspecies in a single step. The assay was standardized using cultured strains and successfully used to analyze the abomasal liquid of aborted bovine fetuses without any pre-enrichment step. Results of our assay were completely consistent with those of traditional bacteriological diagnostic methods. Furthermore, the multiplex PCR technique we developed may be easily adopted by any molecular diagnostic laboratory as a complementary tool for detecting C. fetus subspecies and obtaining epidemiological information about abortion events in cattle.  相似文献