Identification of a cluster of oligonucleotide repeat sequences and its practical implication in melon (<Emphasis Type="Italic">Cucumis melo</Emphasis> L.) breeding     

Hyojung Kim  Jongyeul Baek  Yeon-Ok Choi  Jeong Hyun Lee  Soon-Kee Sung  Sunggil Kim 《Euphytica》2010,171(2):241-249

A cluster of oligonucleotide repeat sequences was identified that is linked to the melon (Cucumis melo L.) andromonoecious (a) locus, which controls andromonoecy. Six different repeat units, ranging from 11 to 49 base pairs, were clustered within a 0.6-kb intergenic region. The number of repeat units varied from two to six. After sequence analysis of diverse melon germplasms, four haplotypes of this cluster were identified. Length variations among the four haplotypes resulted from insertion or deletion of repeat sequences. Particularly, a tandem array of six repeats of 21 nucleotides was a hotspot for insertion/deletion mutations. A simple PCR-based marker was developed to identify haplotypes of this cluster based on the length polymorphism. In practice, this marker was successfully used in genetic purity tests of melon F₁ hybrid cultivars. Four self-pollinated contaminants, which were confirmed by phenotypic examination in grow-out tests, were easily discriminated from 99 F₁ hybrid individuals. In addition, the genetic distance between this marker and the andromonoecious (a) locus was calculated as 7.9 cM, after analyzing melon F₂ populations originating from a cross between monoecious and andromonoecious parental lines. Therefore, this marker will be useful as a recombinant selection marker in marker-assisted backcrossing of monoecy in melon breeding programs.  相似文献   

Comparisons of bearing properties for various oriented glulam using digital image correlation     

Gi Young Jeong  Jin Hyuk Kong  Sang Joon Lee  Sung-Jun Pang 《Journal of Wood Science》2018,64(3):237-245

The goal of this study was to analyze the bearing properties of the differently oriented glulam using digital image correlation (DIC). Six differently oriented specimens associated with three anatomical directions including longitudinal (L), radial (R), and tangential (T), and 12-mm drift pins were used to analyze the bearing properties, including yield load and bearing strength. The highest bearing strength of 22.57 MPa from RL was found, whereas the lowest bearing strength of 6.47 MPa from LR was found. Different strain distributions were observed from the differently oriented bearing specimens using DIC. Different failure ratios of the differently oriented specimens were highly related to the strain distributions. Although the bearing properties were found to be different between the differently oriented specimens, for the connection design aspect, the bearing properties of glulam could be grouped as RL and TL specimens, RT and TR specimens, and LR and LT specimens.  相似文献   

Pharmacokinetics and Acid Suppressant Efficacy of Esomeprazole after Intravenous,Oral, and Subcutaneous Administration to Healthy Beagle Dogs          下载免费PDF全文

J.‐H. Hwang  J.‐W. Jeong  G.‐H. Song  T.‐S. Koo  K.‐W. Seo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(3):743-750

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Marker-assisted identification of resistance genes to soybean mosaic virus in soybean lines     

Jung-Kyung Moon  Soon-Chun Jeong  Kyujung Van  M. A. Saghai Maroof  Suk-Ha Lee 《Euphytica》2009,169(3):375-385

Soybean plants react differentially to soybean mosaic virus (SMV) strains because of interactions among different resistant genes in the soybean genome. Three independent genes resistant to SMV have been identified by inheritance studies and linkage analyses. To develop durable SMV-resistant soybean cultivars, it is necessary to determine which soybean SMV resistance genes can be readily transferred from resistant to susceptible cultivars in a breeding system. Here, we report the type and number of resistance gene(s) in four Korean elite soybean cultivars using a combination of disease reaction symptoms, inheritance studies, and molecular marker mappings. The disease reactions of Sowonkong and Keunolkong soybean varietals in response to infection with SMV strains suggested that both cultivars most likely harbor the Rsv1 gene similar to that in York. Subsequent inheritance studies confirmed that Sowonkong has the Rsv1 gene. The inheritance studies suggested that Sinpaldalkong harbored the Rsv1 gene, which was then confirmed by molecular marker mapping. The inheritance studies also suggested that Jinpumkong 2, which is the most resistant to SMV infection among the four cultivars, contained the Rsv1 and Rsv3 genes; this was confirmed by molecular marker mapping. Our approach, which combined inheritance studies and molecular linkage analyses, allowed the efficient identification of resistance gene(s) in four Korean soybean cultivars.  相似文献   

Anti-inflammatory effect of sweetfish-derived protein and its enzymatic hydrolysate on LPS-induced RAW264.7 cells via inhibition of NF-κB transcription     

Nak-Yun Sung  Pil-Moon Jung  Minchul Yoon  Jeong-Soo Kim  Jong-il Choi  Hye Gwang Jeong  Ju-Woon Lee  Jae-Hun Kim 《Fisheries Science》2012,78(2):381-390

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Promising antidiabetic potential of fucoxanthin isolated from the edible brown algae Eisenia bicyclis and Undaria pinnatifida     

Hyun Ah Jung  Md. Nurul Islam  Chan Mee Lee  Hyong Oh Jeong  Hae Young Chung  Hee Chul Woo  Jae Sue Choi 《Fisheries Science》2012,78(6):1321-1329

In the present study, we investigated the antidiabetic potential of fucoxanthin via the inhibition of rat lens aldose reductase (RLAR), human recombinant aldose reductase (HRAR), advanced glycation end-product (AGE) formation, protein tyrosine phosphatase 1B (PTP1B), and α-glucosidase. Fucoxanthin displayed potent inhibitory activity against AGE formation and HRAR and RLAR activity. In addition, fucoxanthin showed potent inhibitory activity against PTP1B. However, it did not show α-glucosidase inhibitory activity below 200 μM. In addition, our kinetic study revealed that fucoxanthin competitively inhibited RLAR, while it showed mixed-type inhibition against PTP1B. In order to confirm enzyme inhibition, we predicted the 3D structure of PTP1B using Autodock 4.0 to simulate the binding of fucoxanthin. Docking simulation results demonstrated that three residues of PTP1B (Phe30, Phe52, and Gly183) interact with the two hydroxyl groups of fucoxanthin. In addition, the binding energy was negative (?7.66 kcal/mol), indicating that the three hydrogen bonds may stabilize the open form of the enzyme and potentiate tighter binding to the active site of PTP1B, resulting in more effective PTP1B inhibition. The results of the present study therefore clearly demonstrate the promising potential of fucoxanthin as a therapeutic intervention for the management of diabetes as well as diabetes-associated complications, which could be explored further.  相似文献   

Corneal papilloma in a dog     

Kim MS  Kweon DH  Yi NY  Lee JM  Jeong MB  Kang MS  Jeong SM  Nam TC  Kim DY  Seo KM 《The Veterinary record》2005,156(14):454

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Suppression of cytokine-inducible nitric oxide synthesis during intraperitoneal Meth A tumor growth     

Kwon OD  Yim CY  Jeong KS  Park SC  Jung KY  McGregor JR  Bastian NR  Samlowski WE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):357-365

Nitric oxide (NO*) synthesis is induced within many tumors. The time course of NO* synthesis was evaluated during intra-peritoneal Meth A fibrosarcoma progression. While increasing macrophage recruitment into ascites was noted, inducible nitric oxide synthase (iNOS) antigen and function peaked between days 3-6 after tumor implantation. The capacity of cells to respond to LPS and IFNgamma stimulation was markedly depressed on day 9 and 11. Cellular proliferation correlated in an inverse fashion with levels of NO* synthesis. Electron paramagnetic resonance spectroscopy and nitrotyrosine immunostaining failed to show accumulation of characteristic target cell lesions induced by NO*. These findings lead us to conclude that NO* production was increasingly suppressed during Meth A tumor progression. Depression of NO* production did not correlate with levels of the inhibitory cytokines TGFbeta and IL-10, but could be partially overcome by addition of sepiapterin (a tetrahydrobiopterin prodrug). Thus, depletion of essential co-factors necessary for iNOS function may contribute to depressed NO* responses during cancer progression.  相似文献   

Reverse effects of tetraarsenic oxide on the angiogenesis induced by nerve growth factor in the rat cornea     

Yoo MH  Kim JT  Rhee CH  Park MJ  Bae IJ  Yi NY  Jeong MB  Jeong SM  Nam TC  Seo KM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1091-1095

To compare the antiangiogenic effects of tetraarsenic oxide (As4O6) with those of diarsenic oxide (As2O3) in the rat cornea, rat cornea micropocket assay was conducted to induce angiogenesis by implantation of the pellet contained 1.0 ng of nerve growth factor (NGF). Ten of thirty eyes of Sprague-Dawley rats were randomly assigned to one of three groups, namely, control group (no medication), As2O3 group (50 mg/kg As2O3, PO, s.i.d.), and As4O6 group (50 mg/kg As4O6, PO, s.i.d.). After implantation, the number of new vessels, vessel length and clock hour of neovascularization were examined under the microscope from day 3 to day 7. The area of neovascularization was calculated using a mathematical formula. Although new vessels in control and As2O3 groups were first noticed at day 3, whereas those of As4O6 group were first observed on day 5. The number, length, clock hour of neovascularization and areas of the vessels in As4O6 group showed more significant inhibition than those of control and As2O3 groups from day 5 (P<0.05). However, there were no differences in all parameters between control group and As2O3 group during the entire study period. These results showed that As4O6 had antiangiogenic effects on the new vessels induced by NGF in the rat cornea.  相似文献   

Fetal bladder outlet obstruction in a stillborn bovine fetus     

Jeong WI  Lee CS  Chung JY  Park SJ  An MY  Jeong DH  Do SH  Noh DH  Williams BH  Jeong KS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):393-395

A stillborn bovine male fetus with abdominal distention, arthrogryposis and atresia ani was presented for diagnostic evaluation. At necropsy, this fetus had a large amount of ascites, urachal obstruction and marked bladder distention. The ventral surface of the bladder had ruptured and attached to the abdominal wall by fibrinous adhesions. There was bilateral hydronephrosis with moderate pelvic dilatation and cortical attenuation. The rectum was filled with meconium but the anus was imperforate. The right forelimb was contracted. The cause(s) of these abnormalities could not be determined; however, we believe that developmental abnormalities during embryogenesis may be the result of chromosomal abnormalities.  相似文献