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101.
Landeg F 《The Veterinary record》2002,150(9):287-288
102.
103.
Parmentier HK Baelmans R Nieuwland MG Dorny P Demey F 《Veterinary immunology and immunopathology》2002,90(1-2):91-100
Antibody responses, serum complement haemolytic activity, and complement component C3 and Factor B consumption were studied in chickens divergently selected for high and low antibody responses to sheep red blood cells, and in a randombred control line. Significantly higher total and IgG antibody responses to SRBC were found after intramuscular immunisation in the high antibody responder (H) line versus the low antibody responder (L) line and the control (C) line. Also significantly higher antibody titres were found in the C line as compared to the L line. Ca-dependent (classical) and Ca-independent (alternative) complement haemolytic activity was significantly higher in the H line than in the L line. Also initial complement haemolytic activity and C3 levels prior to immunisation with SRBC were significantly higher in the H than in the L line. The L line, on the other hand, showed numerically higher Factor B levels. Immunisation with SRBC was followed by a different consumption of C3 in serum of the H line than the L line.The results indicated that divergent selection of chickens for specific antibody responses to SRBC affected complement levels and C3 consumption in these chickens. This suggests a genetic linkage between these two immune traits. 相似文献
104.
105.
Poirier VJ Thamm DH Kurzman ID Jeglum KA Chun R Obradovich JE O'Brien M Fred RM Phillips BS Vail DM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(6):726-731
The purpose of this randomized, multicenter study was to evaluate the toxicity and efficacy of liposome-encapsulated doxorubicin (LED) and doxorubicin (DOX) in the treatment of feline vaccine-associated sarcoma (VAS). Cats were divided according to their disease status into a microscopic arm (no evidence of gross disease) and a macroscopic arm (evidence of gross disease). Each arm was randomized to receive either LED (1-1.5 mg/kg i.v. q3 weeks) or DOX (1 mg/kg i.v. q3 weeks). Thirty-three cats were entered in the macroscopic arm of the study with an overall response rate of 39% (5 complete response and 8 partial response) and a median time to progression of 84 days. Response rates were not different between LED and DOX. Seventy-five cats were entered into the microscopic arm. When compared to a similar historical control population treated with surgery alone, the cats receiving chemotherapy had a prolonged median disease-free interval (388 days versus 93 days). No difference in efficacy was detected between LED and DOX. LED at 1.5 mg/kg induced delayed nephrotoxicosis in 23%, necessitating a decrease in the recommended dosage to 1 mg/kg, and cutaneous toxicosis in 21.7% of treated cats. This study showed that both DOX and LED are efficacious in the treatment of VAS and should be considered in the treatment of this tumor. 相似文献
106.
Rolf T. Folkertsma Koen E. de Groot Paul H. G. van Koert Marga P. E. van Gent-Pelzer Jeroen N. A. M. Rouppe van der Voort Arjen Schots Jaap Bakker Fred J. Gommers Johannes Helder 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(6):577-584
Thirty-six populations of the potato cyst nematodeGlobodera pallida, all collected in the Netherlands, were analysed twice: by two-dimensional gel electrophoresis of proteins (2-DGE) and by random amplified polymorphic DNA fingerprinting (RAPD). Two-DGE revealed frequencies of 21 alleles at eight putative loci in each population. The same populations were subjected to RAPD analysis. This qualitative technique revealed 38 polymorphic DNA fragments. Both datasets were independently processed to determine the intraspecific variation. UPGMA analysis resulted in a 2-DGE- and a RAPD-based dendrogram with cophenetic correlation coefficients of 0.755 and 0.838 respectively. The correlation between the genetic similarity values for the populations was 0.572. Comparison between the 2-DGE- and the RAPD-based dendrogram revealed that only thirteen of the 36 populations analysed were clustered identically. It is concluded that the gene pool similarity concept is only in some instances applicable to Dutch populations ofG. pallida. For populations that could not be differentiated unequivocally on the basis of molecular markers, markers closely linked to avirulence genes should be identified. Approaches that will lead to the identification of such markers are discussed. 相似文献
107.
A number of chlorinated 1,2-, 1,3- and 1,4-benzodicarbonitriles monosubstituted by either hydroxy, arylamino, alkylthio, sulphinyl or sulphonyl groups were synthesised and assayed against P. viticola, P. oryzae, V. inaequalis, P. parasitica and a number of other fungi and bacteria. The 1,3-benzodicarbonitriles were the most active compounds and the alkylsulphiyl and sulphonyl groups the most effective substituents. 相似文献
108.
Peter Donkin John Widdows Sheila V. Evans Fred J. Staff Tie Yan 《Pest management science》1997,49(2):196-209
The effects of selected neurotoxic pesticides on the feeding rate of marine mussels (Mytilus edulis) were determined. Two organochlorine pesticides, lindane and endrin, two acetylcholinesterase-inhibiting compounds, dichlorvos and carbaryl and two pyrethroids, flucythrinate and permethrin, were studied. No evidence was found for any specific neurotoxic effect of the organochlorines and pyrethroids on feeding efficiency. In contrast, dichlorvos and carbaryl inhibited the enzyme acetylcholinesterase in mussel gills and were more toxic to feeding efficiency than could be explained by a narcotic mechanism of toxicity alone. Dichlorvos also caused clear behavioural changes in the mussels. The significance of these observations for the application of mussels to impact assessment in the marine environment is discussed. © 1997 SCI. 相似文献
109.
David G. Heckel Linda J. Gahan Fred Gould Joanne C. Daly Stephen Trowell 《Pest management science》1997,51(3):251-258
Genetic linkage maps of Heliothis virescens and Helicoverpa armigera are being used to identify and characterize resistance-conferring genes. The insensitive acetylcholinesterase conferring resistance to organophosphorus insecticides and the insensitive sodium channel conferring resistance to pyrethroids have both been mapped in H. virescens. The linkage mapping approach permits a genetic dissection of resistance, even when the mode of action and lethal target are not precisely known, such as for the insecticidal toxins from the bacterium Bacillus thuringiensis (Bt). We have identified and mapped a major Bt-resistance locus in a strain of H. virescens exhibiting up to 10000-fold resistance to Cry1Ac toxin and are currently developing a linkage map for H. armigera with a set of ‘anchor’ loci to facilitate comparison with H. virescens. Both species are currently experiencing their first significant selective pressure in the field by transgenic cotton expressing Cry1Ac, and timely identification of resistance mechanisms and their underlying genetic basis will be essential in successfully managing the Bt resistance that will eventually appear. ©1997 SCI 相似文献
110.
Participatory evaluation of integrated pest and soil fertility management options using ordered categorical data analysis 总被引:1,自引:0,他引:1
Maize is becoming the major food crop around Lake Victoria. Major constraints to its production are Striga, stem borer, and declining soil fertility. Innovative integrated technologies have been developed: the ‘push-pull’ system (intercropping with Desmodium and surrounded by Napier grass), soybean and Crotalaria rotations, and imidazolinone-resistant (IR) maize seed.In 12 demonstration trials in four villages in Siaya and Vihiga districts (Kenya) and two villages in Busia (Uganda) in 2003 and 2004, 504 farmers evaluated all cropping systems and a mono-cropped continuous maize, each cropped with IR or local maize, and supplemented or not with fertilizer, totaling 16 treatments. Farmers evaluated all treatments for yield, resistance to Striga and stem borer, improvement of soil fertility, and provided an overall evaluation score, using an ordered scale of 1 (very poor) to 5 (very good). Data were analyzed using ordinal regression, estimating log odds ratios.The results show significant preferences for all treatments over the control. Push-pull with IR and fertilizer had the highest log odds ratio (2.93), so the odds of farmers preferring this treatment are 18.7 times the odds that farmers prefer the control. The odds ratios for the other push-pull combinations were generally highest (9-15), followed by the rotation systems with Crotalaria (3.5-7.0), and soybeans, especially with IR maize and fertilizer (odds ratio of 5.7). In mono-cropping systems, IR maize was only appreciated in combination with fertilizer, and then only in 2004. Push-pull and Crotalaria were more appreciated in 2004 than in 2003. Farmers in Vihiga had a stronger preference for push-pull, and those in Busia for soybean rotations.Significant differences among farmers were observed, but the effects were small. Women appreciated push-pull more than men, while other technologies were gender-neutral. Older farmers were more likely to prefer push-pull and Crotalaria with fertilizer. Livestock ownership was not found to have an effect on technology preferences. Measured yield, stem borer and Striga infestation all had significant but small effects, although their inclusion did not eliminate the treatment effects, indicating that other factors are still important. OLS of the scores for different criteria on the overall score shows yield to be the most important criterion (coefficient of 0.40), followed by soil fertility enhancement (0.25) and Striga resistance (0.13). Labor saving (0.09) and stem borer resistance (0.03) are relatively minor criteria.This research shows that scoring and analysis with ordinal regression is a convenient way to solicit and analyze farmers’ preferences for new technologies, with wide applicability in farming systems and participatory research. Its application here shows that farmers like the new technologies, especially push-pull, but that there are substantial differences between years, sites and farmers. The use of this method can be very helpful to define and focus further research and formulate specific and targeted recommendations for agricultural extension. 相似文献