Lethal effect of VEGFR₂shRNA on HL60 cells in vitro using lentiviral vector     

GUO Hai-xia  LI Wen-yi  XU Ling  SU Hao-bin  LI Yang  XIA Yan  LIANG Li-yang 《园艺学报》2007,23(6):1053-1057

AIM: To look for harmfulless anti-leukemia drug with selective high performance, lethal effect of small hairpin RNA (shRNA) on VEGFR₂ gene expression of tumor cell line HL60 in vitro.METHODS: The most effective VEGFR₂ siRNA was designed and screened. The shRNA oligo was designed and pU6/VEGFR₂ entry clone was constructed. HL60 was transfected transiently and vascular endothelial growth factor receptor 2(VEGFR₂) expression was tested with MTT assay, RT-PCR and Western blotting. The expression clone was constructed and cotransfected with ViraPowerTM Packaging Mix into 293FTTM cells to produce Lentiviral vectors harboring Lenti6/shVEGFR₂. The virion supernatant was added into HL60 cells and VEGFR₂ gene inhibitory effect was determined. RESULTS: The inhibitory rates of VEGFR₂ siRNA c were high. VEGFR₂ expression in HL60 was inhibited by using pU6/VEGFR₂ entry clone constructed with shRNA and pENTRTM/U6. For HL60 cells, the inhibitory rate was 84.9%. The expression of VEGFR₂ mRNA and protein decreased significantly. 48 hours after transfection of pU6/shVEGFR₂ entry clone and transduction of Lenti6/shVEGFR₂ expression clone, the cell inhibitory rates were similar. Cell growth inhibitory rate of entry clone descended rapidly after this time point, the expression clone changed slowly, reaching the peak at 96 hours, dropped slightly, having no significance deviation. CONCLUSION: in vitro, VEGFR₂ shRNA using lentiviral vector blocks VEGF/VEGFR₂ self-secretion in HL60 cells, which inhibits leukemia development.  相似文献   

Effects of PDS on rat brain cortical nuclear factor kappa B in LPS shock     

WANG Zhi  LI Yang  SU Jing  ZHOU Li  JI Hai-ting  SUN Lian-kun 《园艺学报》2007,23(8):1540-1542

AIM:To explore the molecular mechanism of brain tissue injury induced by lipopolysaccharide (LPS),the effects of panaxadiol (PDS) on the expression of nuclear factor kappa B (NF-κB) in cerebral cortex of rat with LPS shock were studied. METHODS:Rats were randomly divided into LPS roup,LPS+dexamethasone group,LPS+PDS group and control group. The DNA binding activity and protein expression of NF-κB were observed. These indices were assayed at 1 h and 4 h after intravenous injection of LPS (4 mg·kg^-1). RESULTS:EMSA showed that PDS inhibited NF-κB DNA-binding activity in nuclear extracts at both 1 h and 4 h after LPS injection,compared with the LPS group (P<0.01). Western blotting showed that PDS down-regulated the expression of p65 and p50 protein in the nuclear extracts compared with the LPS group. However,the expression of p65 and p50 protein from cytosolic extracts did not show any significant change. CONCLUSION:PDS may alleviate brain injury by inhibiting NF-κB activation.  相似文献   

Expression of AFP and MXR7 mRNA in the peripheral blood and its relationship with prognosis of patients with hepatocellular carcinoma following liver transplantation     

YI Shu-hong  WANG Gen-shu  CAI Chang-jie  LU Min-qiang  YANG Yang  HU Bin  CHEN Gui-hua 《园艺学报》2007,23(11):2158-2163

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用放射免疫法检测家蚕雌蛾体中雌二醇的含量   总被引：1，自引：2，他引：1  

贺伟强  顾燕燕  时连根 《蚕业科学》2007,33(2):302-306

为了便于对大量家蚕雌蛾粉及其制品样本中雌二醇的含量进行快速测定,研究了家蚕雌蛾体中雌二醇含量的测定方法。以0.1 mol/L HCl为处理液,乙醚为提取溶剂,振荡重复抽提3次,可有效地提取家蚕雌蛾体中雌二醇。以90 g/L牛血清白蛋白Tris缓冲液为测定溶剂,采用均相竟争的放射免疫法,可精确、稳定地测定出家蚕雌蛾体中雌二醇含量。  相似文献   

不同复种方式下作物的粗蛋白和可消化干物质总产量比较   总被引：1，自引：0，他引：1  

钟小仙  顾洪如  江海东  曹卫星 《草业科学》2007,24(4):75-78

在南京对6种不同复种方式下作物的营养物质产量进行了比较研究,结果表明:多花黑麦草Lolium multiflorum粗蛋白总产量和可消化干物质总产量分别是小麦Triticum eastivum的1.45倍和1.26倍;杂交狼尾草Pennisetum americanum×P.purpureum粗蛋白总产量和可消化干物质总产量平均值分别是水稻Oryza sativa的2.06倍和1.61倍;6种复种方式中,粗蛋白总产量和可消化干物质总产量以多花黑麦草-杂交狼尾草为最高,黑麦Secale cereal-杂交狼尾草其次.集约农区发展草食性畜禽养殖业以二季均种植牧草效率较高.  相似文献   

遵义市辣椒产业发展的现状、问题与对策   总被引：2，自引：0，他引：2  

封兴智  詹永发  杨红  赖卫  余文中  涂祥敏 《辣椒杂志》2007,5(2):2-4,7

本文概述了遵义市辣椒产业发展的基本情况及存在的问题,进一步提出了当前遵义辣椒产业发展的思路与措施。  相似文献   

Th〖ZK(〗e effect of L-phenylalanine on the plasma NO,ET levels and SOD activity in spontaneous hypertensive rats     

CHE Zai-qian  FAN Chun-ling  GU Tian-hua  WU Yong-jie  ZHAO Guang-sheng 《园艺学报》2007,23(2):389-390

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Hemin induces and sustains the phosphorylation of Erk1/2 in human umbilical vascular endothelial cells     

CHEN Nan  CHEN Ying-ying  XU He-jing  WANG Yang  ZHU Li  SHEN Yue-liang 《园艺学报》2007,23(4):652-655

AIM: To investigate the relationship between hemin and Erk1/2 activation in human umbilical vascular endothelial cells (HUVECs). METHODS: Cultured HUVECs were separately incubated with hemin or H₂O₂ for different times. Subsequently Erk1/2 phosphorylation and total Erk1/2 were determined by Western blotting assay. Flow cytometry was employed to determine the cell cycle distribution. RESULTS: Hemin at the concentration of 1-10 μmol/L induced the phosphorylation of Erk1/2 in HUVECs, and sustained the phosphorylation of Erk1/2 for three hours. The duration of phospho-Erk1/2 induced by hemin was much longer than that in H₂O₂ control (3 h vs 30 min). CONCLUSION: Hemin induces and sustains the phosphorylation of Erk1/2 in HUVECs, which indicates that the effect of hemin on the Erk1/2 activation may be one of pharmacological target of hemin.  相似文献   

Adipocyte-derived relaxing factor,a new vasodilative factor     

WANG Hao-fan  WANG Hua-dong  QU Yang 《园艺学报》2007,23(8):1649-1653

Although our knowledge of ADRF (adipocyte-derived relaxing factor) is extremely limited,the little that is known has already revealed a promising future in this newly found factor.Firstly,it is secreted by adipose tissue,which is a abundantly and extensively distributed in human body and has become a hot spot for research work in recent years.Secondly,ADRF has shown a significant vasodilative action in a considerable number of experiments conducted on arteries of various sizes,from different body parts of different species of animals,including human.In this article,we introduce the development of ADRF research,sum up its known properties,including its Ca2+,protein tyrosine kinase,and protein kinase A dependent releasing,K+ channel mediated functioning,and interfered effect in different pathological models,and propose problems surrounding this factor and directions for future research work.  相似文献   

芸香科及樟科8种植物提取物对荔枝蒂蛀虫的产卵驱避作用          下载免费PDF全文

杨长龙  ;江世宏;陈晓琴 《植物保护》2007,33(6):57-59

测试了芸香科及樟科8种植物的乙醇提取物对荔枝蒂蛀虫的产卵驱避作用,发现四季橘和沙田柚提取物对该虫产卵具有较高的驱避作用,选择性驱避率分别为84.26%、53.90%,非选择驱避率分别为90.14%、60.83%;而九里香提取物则对荔枝蒂蛀虫的产卵具有一定的引诱作用。对四季橘提取物用不同的溶剂初步分离后发现,氯仿层和乙酸乙酯层萃取物对该虫的驱避作用最高,分别为84.67%、86.63%,即其活性成分主要分布在氯仿层和乙酸乙酯层萃取物中。  相似文献