The genome of the kinetoplastid parasite, Leishmania major     

Ivens AC  Peacock CS  Worthey EA  Murphy L  Aggarwal G  Berriman M  Sisk E  Rajandream MA  Adlem E  Aert R  Anupama A  Apostolou Z  Attipoe P  Bason N  Bauser C  Beck A  Beverley SM  Bianchettin G  Borzym K  Bothe G  Bruschi CV  Collins M  Cadag E  Ciarloni L  Clayton C  Coulson RM  Cronin A  Cruz AK  Davies RM  De Gaudenzi J  Dobson DE  Duesterhoeft A  Fazelina G  Fosker N  Frasch AC  Fraser A  Fuchs M  Gabel C  Goble A  Goffeau A  Harris D  Hertz-Fowler C  Hilbert H  Horn D  Huang Y  Klages S  Knights A  Kube M  Larke N  Litvin L  Lord A 《Science (New York, N.Y.)》2005,309(5733):436-442

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Effect of Phosphorus and Arbuscular Mycorrhizal Fungi (AMF) Inoculation on Growth and Productivity of Maize (Zea mays L.) in a Tropical Ferralsol     

Tshibangu Kazadi  Audry  Lwalaba wa Lwalaba  Jonas  Kirika Ansey  Bibich  Mavungu Muzulukwau  Judith  Manda Katabe  Gabriella  Iband Karul  Martine  Baert  Geert  Haesaert  Geert  Mukobo Mundende  Robert-Prince 《Gesunde Pflanzen》2022,74(1):159-165

Soil fertility in the Lubumbashi region often proves to be limiting factor for crop production due to their low nutrient reserves. The objective of this work was to evaluate the impact of arbuscular mycorrhizae on phosphorus uptake by maize on Ferralsol. The trial was conducted in pots with 30?kg or 60?kg of P₂O₅ ha^?1 and a control. These three levels of phosphorus were combined or not with arbuscular mycorrhizae. The combinations of 30?kg or 60?kg of phosphorus with the inoculum led to a male flowering of maize at 63 days after semi. Maize treated with 60?kg of phosphorus ha^?1 formed very few or almost no blisters in the roots. Cob weight, length, diameter, number of rows and kernel weight varied significantly with phosphorus on both inoculated and uninoculated pots. The inoculated plants had high averages for these yield parameters. Due to the lack of phosphate fertilizer, inoculum alone can be an alternative to phosphorus provided that nitrogen and potassium are added, resulting in small but seed-filled ears compared to the phosphorus-free control without mycorrhizae, which resulted in empty ears. Yield varied significantly with the addition of phosphorus (0.3 to 6.1 tons ha^?1) and less significantly with inoculum (3 to 3.7?t ha^?1). The combination of treatments showed a significant difference in favour of the 60?kg of phosphorus or 60?kg of phosphorus associated with the inoculum. The highest phosphorus content was obtained on the inoculum treatment alone, which provided 1.4?mg phosphorus?g^?1 maize compared to other treatments, which provided 0.69 to 0.71?mg phosphorus?g^?1 maize.

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Identification of artichoke SSR markers: molecular variation and patterns of diversity in genetically cohesive taxa and wild allies     

Gabriella Sonnante  Anna Vittoria Carluccio  Angelo De Paolis  Domenico Pignone 《Genetic Resources and Crop Evolution》2008,55(7):1029-1046

A set of 24 microsatellite markers was identified in the artichoke genome, using various approaches. A genomic library allowed the development of 14 SSR markers, whereas the other 10 were obtained from gene intron/UTR regions or from other species. Allelic variation was scored in C. cardunculus (artichoke, cultivated cardoon, and wild cardoon) samples, and in other wild Cynara allies. For the 23 polymorphic loci, a total of 165 alleles were scored, 135 of which in the artichoke primary genepool, and the remaining ones in the other Cynara species. Some allele combinations were able to identify artichoke varietal types, and some alleles were unique to specific groups. This makes these markers potentially useful in product traceability and in contributing to the saturation of genetic maps. The percentage of shared alleles between C. cardunculus taxonomic groups, and Nei’s genetic distances indicated that wild cardoons from the Eastern Mediterranean were more closely related to artichoke and less to cultivated cardoon in comparison to wild cardoons from the Western Mediterranean, and the genetic distance between the two wild cardoon genepools was rather high. The UPGMA dendrogram based on Nei’s genetic distances revealed that artichokes formed a fairly defined cluster, whereas Eastern wild cardoons occupied another branch, and Western wild cardoons were clustered together with cultivated cardoons. The transferability of microsatellite markers to other Cynara wild species was quite good. Sequencing alleles at three loci showed that, apart from microsatellite length variation, point mutations and insertion/deletions were quite abundant especially when comparing C. cardunculus to the other Cynara species. In the sequenced regions, some SNPs were identified which distinguished artichoke on one side, and cultivated and wild cardoon on the other, while other SNPs were apportioned according to the geographic distribution of Cynara wild species.  相似文献   

Isolation of an in vitro and ex vivo antiradical melanoidin from roasted barley     

Papetti A  Daglia M  Aceti C  Quaglia M  Gregotti C  Gazzani G 《Journal of agricultural and food chemistry》2006,54(4):1209-1216

The antiradical properties of water-soluble components of both natural and roasted barley were determined in vitro, by means of DPPH* assay and the linoleic acid-beta-carotene system, and ex vivo, in rat liver hepatocyte microsomes against lipid peroxidation induced by CCl4. The results show the occurrence in natural barley of weak antioxidant components. These are able to react against low reactive peroxyl radicals, but offer little protection against stable DPPH radicals deriving from peroxidation in microsomal lipids. Conversely, roasted barley yielded strong antioxidant components that are able to efficiently scavenge free radicals in any system used. The results show that the barley grain roasting process induces the formation of soluble Maillard reaction products with powerful antiradical activity. From roasted barley solution (barley coffee) was isolated a brown high molecular mass melanoidinic component, resistant to acidic hydrolysis, that is responsible for most of the barley coffee antioxidant activity in the biosystem.  相似文献   

Antioxidant composition in cherry and high-pigment tomato cultivars     

Lenucci MS  Cadinu D  Taurino M  Piro G  Dalessandro G 《Journal of agricultural and food chemistry》2006,54(7):2606-2613

Fourteen cultivars of cherry tomatoes and four cultivars of high-pigment tomato hybrids were cultivated in southern Italy, and the red-ripe fruits were analyzed for their content in different classes of antioxidants and for their antioxidant activity. Among the different cultivars, significant differences were found between lycopene, beta-carotene, alpha-tocopherol, vitamin C (ascorbic acid and dehydroascorbic acid), and total phenolic and flavonoid contents. LS203 and Corbus appear to be the cultivars with the highest content of lipophilic and hydrophilic antioxidants among cherry tomatoes, respectively. All cultivars of high-pigment tomato hybrids showed an expected exceptionally high lycopene content. Among them, the highest content of lipophilic and hydrophilic antioxidants was found in cv. HLY 13. Hydrophilic and lipophilic antioxidant activities were both significantly influenced by genotype. Such results highlight an existing unexploited variability in tomato germplasm and stress the need to evaluate the biodiversity and to support conventional breeding programs to improve tomato nutritional value.  相似文献   

First evidence of resistance to pyrethroid insecticides in Italian Aedes albopictus populations 26 years after invasion          下载免费PDF全文

Verena Pichler  Romeo Bellini  Rodolfo Veronesi  Daniele Arnoldi  Annapaola Rizzoli  Riccardo Paolo Lia  Domenico Otranto  Fabrizio Montarsi  Sara Carlin  Marco Ballardini  Elisa Antognini  Marco Salvemini  Emanuele Brianti  Gabriella Gaglio  Mattia Manica  Pietro Cobre  Paola Serini  Enkelejda Velo  John Vontas  Ilias Kioulos  Joao Pinto  Alessandra della Torre  Beniamino Caputo 《Pest management science》2018,74(6):1319-1327

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Eco-restoration of a mine technosol according to biochar particle size and dose application: study of soil physico-chemical properties and phytostabilization capacities of <Emphasis Type="Italic">Salix viminalis</Emphasis>     

Manhattan Lebrun  Florie Miard  Romain Nandillon  Gabriella S. Scippa  Sylvain Bourgerie  Domenico Morabito 《Journal of Soils and Sediments》2018,18(6):2188-2202

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