Preparation, characterisation and field-testing of a biodegradable sodium alginate-based spray mulch   总被引：1，自引：0，他引：1  

Barbara Immirzi  Gabriella Santagata  Giuliano Vox  Evelia Schettini 《Biosystems Engineering》2009,102(4):461-472

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A pith necrosis caused by Xanthomonas perforans on tomato plants     

Dalia Aiello  Giuseppe Scuderi  Alessandro Vitale  Giuseppe Firrao  Giancarlo Polizzi  Gabriella Cirvilleri 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(1):29-41

Pith necrosis is a common disease of tomato in Europe, mainly caused by Pseudomonas corrugata and other soil-borne species of Pseudomonas. During 2011–2012 a survey was conducted in soil-grown tomato crops in southeastern Sicily (Italy). Plants showed pith necrosis, brown discolouration of the vascular tissues, leaf chlorosis and sometimes wilting of leaves. Thirty bacterial isolates from symptomatic tissues, forming colonies on NA and KB, were identified by morphological, biochemical and physiological tests. Among them, seven isolates were analyzed for their 16S rDNA and 16S–23S spacer region sequence that resulted in 99 % identity to that of the Xanthomonas perforans type strain (GenBank accession number GQ46173over 2.085 bp.). Additional sequences of fusA, gapA, gltA, gyrB, lacF, and lepA from one selected isolate were 100% identical to sequences of the Xanthomonas perforans type strain. X. perforans local isolates showed similar genomic patterns with REP-PCR and fAFLP, and were clearly distinguished from other Xanthomonas spp. type strains. In stem-inoculation assays, bacteria isolated from symptomatic tomato plants identified as P. fluorescens, P. putida, P. marginalis, P. citronellolis, P. straminea, and Pantoea agglomerans induced discolouration of vascular tissues, while Pectobacterium carotovorum subsp. atrosepticum isolates induced soft rot. Conversely, the isolates here identified as Xanthomonas perforans were able to induce pith necrosis, vascular discolouration, longitudinal splits and external lesions on stems. This report of X. perforans causing pith necrosis on tomato represents a potentially serious problem that may limit the productivity of tomato crops.  相似文献   

First reports of Trichinella pseudospiralis in wild boars (Sus scrofa) of Italy     

Merialdi G  Bardasi L  Fontana MC  Spaggiari B  Maioli G  Conedera G  Vio D  Londero M  Marucci G  Ludovisi A  Pozio E  Capelli G 《Veterinary parasitology》2011,178(3-4):370-373

Trichinella pseudospiralis is a non-encapsulated species infecting both mammals and birds. In Italy, this parasite was reported only in two night-birds of prey of Central Italy. In January 2010, Trichinella larvae were detected in three wild boars (Sus scrofa) of two regions of Northern Italy by enzymatic digestion. The parasites were identified as T. pseudospiralis by multiplex-PCR. The first infected wild boar was hunted in the Emilia Romagna region and the other two infected wild boars were bred outdoors in a small family farm of the Friuli Venezia Giulia region. These new epidemiological data reinforce the role of the wild boar as the main reservoir of T. pseudospiralis in Europe.  相似文献   

Genome-wide association study for insect bite hypersensitivity susceptibility in horses revealed novel associated loci on chromosome 1     

Merina Shrestha  Marina Solé  Bart J. Ducro  Marie Sundquist  Ruth Thomas  Anouk Schurink  Susanne Eriksson  Gabriella Lindgren 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(2):223-233

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Genetic diversity in Borago officinalis germplasm as revealed by seed oils and AFLP polymorphism     

Antonino De Lisi  Vincenzo Montesano  Donatella Negro  Giulio Sarli  Emanuela Blanco  Gabriella Sonnante  Gaetano Laghetti 《Genetic Resources and Crop Evolution》2014,61(4):853-859

Ten accessions of wild Borago officinalis were collected during missions in the course of a research activity carried out on plants producing nutraceutical compounds. The aim was to identify populations that produce a higher content of γ-linolenic acid, as this compound has important therapeutic properties and has been used in medical tests to treat rheumatoid arthritis, eczema, and psoriasis with good therapeutical results. The present study reports on the characterization of both the polyunsaturated fatty acid composition and on genetic diversity estimated by means of AFLP markers in several B. officinalis accessions collected in Southern Italy and grown in isolation by means of screen houses specifically designed for each single population. Polyunsaturated fatty acid were found in different amounts in all investigated accessions. The populations were very variable at the molecular level, and the analysis of relationships based on AFLP data, showed that similarity based on Jaccard’s index ranged between 0.330 and 0.742. Our investigations allowed us to identify a population that may be considered as a good source of GLA.  相似文献   

Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line     

Caruso MG  Messa C  Orlando A  D'Attoma B  Notarnicola M 《Fitoterapia》2008,79(7-8):524-528

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Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy.     

Nicola Decaro  Marco Campolo  Costantina Desario  Francesco Cirone  Maria D'Abramo  Eleonora Lorusso  Grazia Greco  Viviana Mari  Maria Loredana Colaianni  Gabriella Elia  Vito Martella  Canio Buonavoglia 《Journal of veterinary diagnostic investigation》2008,20(1):28-32

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Pregnancy stage and number of fetuses may influence maternal plasma leptin in ewes     

Kulcsár M  Dankó G  Magdy HG  Reiczigel J  Forgach T  Proháczik A  Delavaud C  Magyar K  Chilliard Y  Solti L  Huszenicza G 《Acta veterinaria Hungarica》2006,54(2):221-234

Maternal plasma leptin is elevated in ewes during pregnancy. The authors studied whether there was any relation between maternal plasma leptin and insulin concentrations, the number of fetuses and the circulating and faecal levels of gestagens. At the end of the breeding season in January the ovarian activity of Prolific Merino ewes was induced/synchronised with gestagen + eCG treatment. Ewes were inseminated artificially (AI) by laparoscopy. Blood and faecal samples were collected before AI (day 0) and again 41, 81 and 101 days later. The plasma levels of leptin (pL), insulin and progesterone (pP4), and the faecal P4 metabolite (P4-met) content were determined. The day 0 level of pL was significantly higher in pregnant (n = 24) than in non-pregnant ewes (n = 32). By day 41 the pL of pregnant animals had doubled, it showed a further moderate increase on day 81, and decreased slightly thereafter. During pregnancy pP4 and faecal P4-met rose continuously and were positively correlated at all stages. The mean levels of pL and pP4 and the faecal content of P4-met were lower in ewes bearing single (n = 12) than in those with 2 (n = 6) or 3-5 fetuses (n = 6). Analysis of variance demonstrated significant differences according to the number of fetuses in the pL and pP4, but not in P4-met (p = 0.042, 0.044, and 0.051, respectively). Leptin showed positive correlation with insulin before the AI but not during pregnancy. On days 41 and 81 pL showed a slight positive correlation with P4 and P4-met, which decreased slightly by day 101. This study shows that although leptinaemia is affected by the number of fetuses and the level of P4, pregnancy stage is a more important regulator than these additional factors.  相似文献   

Courtship and mating behaviour in the fruit fly parasitoid <Emphasis Type="Italic">Psyttalia concolor</Emphasis> (Szépligeti) (Hymenoptera: Braconidae): the role of wing fanning     

Giovanni Benelli  Gabriella Bonsignori  Cesare Stefanini  Angelo Canale 《Journal of pest science》2012,85(1):55-63

Psyttalia concolor is a parasitoid of fruit flies (Tephritidae) which is used to control the olive fruit fly (Bactrocera oleae). Detailed knowledge of its reproductive biology is required to optimise mass rearing conditions and use in biological control. In this study, the mating behaviour of P. concolor was investigated in order to understand the factors that can influence the success of male mating, using both high-speed video and biological assays. P. concolor males were more likely to be successful in mating during the early morning as opposed to the afternoon, and their courtship behaviour was not affected by the female mating status (i.e. virgin or mated). Wing fanning and copulation attempts were also frequently displayed among males, but male mating status did not influence male–male courtship behaviour. Video data revealed that during wing fanning, male wings were kept raised and moved rhythmically in a dorsoventral fashion. Frequency, pulse duration and interpulse interval of the fanning males during female courtship differed significantly in successful mating attempts compared to unsuccessful ones. Wingless males had significantly lower mating success compared to winged males, suggesting that wing fanning increases the likelihood of attracting a mate. Overall, the study increases the knowledge of the reproductive biology of P. concolor and suggests the importance of fanning behaviour among the range of sensory modalities used by this parasitoid in intra-specific communication.  相似文献   

Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques     

Lombardo G  Pennisi MG  Lupo T  Migliazzo A  Caprì A  Solano-Gallego L 《Veterinary parasitology》2012,184(1):10-17

The use of non invasive sampling, such as collection of conjunctival swabs, as a diagnostic tool for the detection of Leishmania DNA is of interest. The purpose of this study was to evaluate the diagnostic utility of detecting Leishmania infection with the use of conjunctival swab samples in dogs living in a highly endemic area for leishmaniosis and to investigate, for the first time, the presence of Leishmania DNA in oral swabs in the same population. One hundred sixty-three dogs living outdoor and recruited in various provinces of Sicily were studied. Leishmania infantum indirect fluorescent antibody test (IFAT), delayed-type hypersensitivity reaction to leishmanin (DTH) and real-time PCR of blood (BL), lymph node (LN), conjunctival (CS) and oral swab (OS) samples were performed. The positive PCR percentages in LN, CS, OS and BL samples were: 24.5%, 22.1%, 8.7% and 5.5%, respectively. Serological and DTH positive percentages were 27.0% and 73.8%, respectively. Seropositive and LN-PCR positive dogs had a high likelihood to be positive by CS-PCR. The similar positive PCR percentages found in CS and LN samples suggest the use of CS-PCR as non-invasive alternative technique to LN-PCR for the detection of Leishmania infection in dogs. In addition, this study demonstrated, for the first time, the presence of Leishmania DNA in oral swabs in dogs.  相似文献