Generational growth rate estimates of <Emphasis Type="Italic">Diabrotica virgifera virgifera</Emphasis> populations (Coleoptera: Chrysomelidae)     

Márk Szalai  Judit Papp Komáromi  Renata Bažok  Jasminka Igrc Barčić  József Kiss  Stefan Toepfer 《Journal of pest science》2011,84(1):133-142

Modelling population dynamics of the maize pest Diabrotica virgifera virgifera LeConte (western corn rootworm; Coleoptera: Chrysomelidae) requires knowledge on the growth rate (=net reproductive rate) of the species. We investigated the generational (=annual) growth rate of D. v. virgifera in isolated maize fields in southern Hungary and eastern Croatia over several years. The population densities of D. v. virgifera were assessed by absolute counts of emerging adults in 90 gauze cages per study field. Emergence ranged from 1.3 to 30.7 adults per m² in continuous maize field sections, and from 0.3 to 5.1 adults per m² in adjacent first-year maize sections. The annual growth rates of D. v. virgifera ranged from 0.5 to 13, and averaged in close to 4. These experimentally assessed growth rates could complement growth estimates in population dynamic models, particularly those for forecasting the population growth to economic thresholds or for estimating population build-ups after new introductions of this alien species in Europe. As an example, the determined growth rate was used to estimate that the first documented successful introduction of this species into Europe occurred between 1979 and 1984, which is 8–13 years before the detection of this species and its larval damage in maize fields near Belgrade, Serbia, in 1992.  相似文献   

In vivo and in vitro propagation and transmission of Toggenburg orbivirus     

Planzer J  Kaufmann C  Worwa G  Gavier-Widén D  Hofmann MA  Chaignat V  Thür B 《Research in veterinary science》2011,91(3):e163-e168

The Toggenburg orbivirus (TOV), a recently discovered virus related to bluetongue virus (BTV), has been identified in goats in Switzerland, Italy and Germany. Isolation of TOV in vitro has not yet been achieved and the transmission mechanisms are still unknown. In the experimental infection of pregnant goats described here, TOV could not be detected in secretion/excretion samples or fetal blood. Material from the goat experiment was used as inoculum for propagating the virus in vitro. To enhance the infectivity of TOV several modified protocols, e.g. pretreatment of the virus with trypsin, polyethylene glycol-mediated infection and lipofection were applied. Isolation of TOV, attempts to infect Culicoides nubeculosus by feeding TOV-positive blood and intracerebral inoculation of newborn mice were unsuccessful. The results of these studies suggest that TOV requires specific but different factors than other BTVs for infection and replication outside of its natural caprine host.  相似文献   

Western European epidemiological survey for parvovirus and coronavirus infections in dogs     

Nicola Decaro  Costantina Desario  Monica Billi  Viviana Mari  Gabriella Elia  Alessandra Cavalli  Vito Martella  Canio Buonavoglia 《Veterinary journal (London, England : 1997)》2011,187(2):195-199

An epidemiological survey for canine parvovirus (CPV) and canine coronavirus (CCoV) infections was conducted in Western Europe. A total of 156 faecal samples were collected from dogs with diarrhoea in Spain (n = 47), Italy (n = 39), France (n = 26), Germany (n = 21), the United Kingdom (n = 8), Belgium (n = 10), and the Netherlands (n = 5). Using molecular assays for virus detection and characterisation, CPV and CCoV were found to be widespread in European dog populations, either alone or in mixed infections. In agreement with previous reports, the original type CPV-2 was shown not to circulate in European dogs. The recently identified virus variant CPV-2c was predominant in Italy and Germany and present at high rates in Spain and France but was not detected in the UK or Belgium. Except for the UK, CCoV genotype I was identified in all European countries involved in the survey, albeit at a lower prevalence rates than CCoV genotype II.  相似文献   

Risk factors for canine neosporosis in farm and kennel dogs in southern Italy     

Paradies P  Capelli G  Testini G  Cantacessi C  Trees AJ  Otranto D 《Veterinary parasitology》2007,145(3-4):240-244

Neosporosis by Neospora caninum causes losses to livestock production through abortion in cattle while, in dogs, it induces neuromuscolar disease. This study investigated neosporosis seroprevalence associated risk factors (including the seropositivity to leishmaniosis) in dogs of southern Italy, determined the prevalence of N. caninum oocyst shedding and examined the relationship between seroprevalence of neosporosis in farm dogs and cattle. Using an inhibition ELISA, 20.9% of 306 dogs had percent inhibition values >10 (indicative of exposure) and farm dogs had a significantly (p<0.001) higher seroprevalence than dogs in a rescue kennel. Whilst N. caninum seroprevalence was associated with increasing age in dogs (p< or =0.01) there was no association between seropositivity for N. caninum and for Leishmania infantum. Oocysts of N. caninum were not detected in faecal samples from 230 dogs including 160 farm dogs. The results indicated that neosporosis infection is common in southern Italy both in dogs and in cattle and that dogs at higher risk of exposure are free-ranging ones living in farms. The lack of correlation between canine seroprevalence for N. caninum and L. infantum assumes a particular significance in an endemic area for leishmaniosis.  相似文献   

Cochliomyia hominivorax myiasis in a colony of stray cats (Felis catus Linnaeus, 1758) in Rio de Janeiro, RJ     

Mendes-de-Almeida F  Labarthe N  Guerrero J  Landau-Remy G  Rodrigues DP  Borja GE  Pereira MJ 《Veterinary parasitology》2007,146(3-4):376-378

Cochliomyia hominivorax infestation in domestic cats of an urban colony in the city of Rio de Janeiro is described. The overall prevalence over the period 2001-2005 was 12.5%. Only adult cats were found infested and among these, most cases were observed in males (28%) (p < or = 0.05). The most frequently infested areas of the cats' bodies were the face and nape of the neck. Most lesions were found on the front part of the body of adult males (80%), suggesting that myiasis occur in consequence of competitive fighting wounds.  相似文献   

Use of Red Blood Cell Membranes to Evaluate the Antioxidant Potential of Plant Extracts   总被引：1，自引：1，他引：0  

Gabriella Cavallini  Marina Dachà  Lucia Potenza  Annamaria Ranieri  Claudia Scattino  Antonella Castagna  Ettore Bergamini 《Plant foods for human nutrition (Dordrecht, Netherlands)》2014,69(2):108-114

Antioxidant phytochemicals in fruits and vegetables of a vegetarian diet may account for the reduced risk of aging and stress oxidative associated diseases. In this study, a simple, rapid and accurate new bioassay for the determination of the antioxidant activity of purified or crude plant extracts and thier interactions is described, based on the fluorimetric determination of thiobarbituric acid reactive substances (TBARS) released by UV-B radiated red blood cell (RBC) ghosts. Pure resveratrol, white and red wine and pomegranate juice (PJ) were used as antioxidant source to test the biological method. TBARS production is a function of radiation time, the number of RBC ghosts in the radiated sample and the loaded antioxidant. The antioxidant activity of resveratrol was detected at a submicromolar concentration range [0.02 μg/mL–0.1 μmol/L]. The activity of red wine was almost 10 times higher than that of white wine, and PJ juice had the highest activity. Submaximal protective effects of PJ and red wine were additive.  相似文献   

Effect of thermal treatment on properties of OSB panels   总被引：2，自引：1，他引：1  

Rafael Farinassi Mendes  Geraldo Bortoletto Júnior  Natalie Ferreira de Almeida  Paula Gabriella Surdi  Isabela Najm Barbeiro 《Wood Science and Technology》2013,47(2):243-256

The main objective of this study was to evaluate the effect of thermal treatment of strand particles (pre-treatment) and panels (post-treatment) on the physical and mechanical properties of OSB. The experimental design consisted of five treatments, with the evaluation of the following: two particle treatment temperatures (473.15 and 513.15 K) for a period of 60 min, thermal post-treatment of the panels produced with and without application of wax emulsion at 493.15 K for a period of 12 min, and panels without heat treatment (control). For each treatment, three panels were produced with adhesives based on phenol formaldehyde. The pressing cycle of the panels was at a pressure of 3.51 MPa, 433.15 K and 8 min. Through the analysis of the results, it was possible to draw the following conclusions: the panels produced with the particles heat-treated at 513.15 K showed the best dimensional stability. The thermal post-treatment caused a significant improvement in some of the physical properties of the panels without compromising the mechanical properties. The thermal pre-treatment provided a more pronounced effect on the panel properties than the thermal post-treatment providing improved physical properties and lower mechanical properties.  相似文献   

Effect of Astaxanthin on Human Sperm Capacitation     

Gabriella Donà  Ivana Ko?uh  Anna Maria Brunati  Alessandra Andrisani  Guido Ambrosini  Guglielmo Bonanni  Eugenio Ragazzi  Decio Armanini  Giulio Clari  Luciana Bordin 《Marine drugs》2013,11(6):1909-1919

In order to be able to fertilize oocytes, human sperm must undergo a series of morphological and structural alterations, known as capacitation. It has been shown that the production of endogenous sperm reactive oxygen species (ROS) plays a key role in causing cells to undergo a massive acrosome reaction (AR). Astaxanthin (Asta), a photo-protective red pigment belonging to the carotenoid family, is recognized as having anti-oxidant, anti-cancer, anti-diabetic and anti-inflammatory properties and is present in many dietary supplements. This study evaluates the effect of Asta in a capacitating buffer which induces low ROS production and low percentages of acrosome-reacted cells (ARC). Sperm cells were incubated in the presence or absence of increasing concentrations of Asta or diamide (Diam) and analyzed for their ROS production, Tyr-phosphorylation (Tyr-P) pattern and percentages of ARC and non-viable cells (NVC). Results show that Asta ameliorated both sperm head Tyr-P and ARC values without affecting the ROS generation curve, whereas Diam succeeded in enhancing the Tyr-P level but only of the flagellum without increasing ARC values. It is suggested that Asta can be inserted in the membrane and therefore create capacitation-like membrane alteration which allow Tyr-P of the head. Once this has occurred, AR can take place and involves a higher numbers of cells.  相似文献   

Changes induced by powdery mildew in the salicylic acid and polyamine contents and the antioxidant enzyme activities of wheat lines     

Magda P��l  Vikt��ria Kov��cs  Gyula Vida  Gabriella Szalai  Tibor Janda 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(1):35-47

Investigations were made on four wheat (Triticum aestivum L.) lines under greenhouse conditions, in order to reveal the role of stress-protective materials, namely salicylic acid, polyamines and antioxidant enzymes in the level of tolerance to powdery mildew infection caused by Blumeria graminis (DC.) Speer f.sp. tritici ém. Marchal. The four lines showed different levels of tolerance, assessed on the Saari-Prescott scoring scale: TC26 and TC33 proved to be susceptible and TC9 and TC19 resistant. In most of the lines, infection caused changes in the activities of antioxidant enzymes, especially in the case of guaiacol peroxidase. Four peroxidase isoenzymes, which responded differently to powdery mildew infection could be detected by gel electrophoresis. Infection had only a slight effect on the levels of salicylic acid (free and bound forms) in inoculated plants; while the levels of polyamines, especially spermidine and spermine increased after infection. Correlation analysis was also performed to examine how close a relationship exists between the parameters investigated. It was concluded that salicylic acid, polyamines and antioxidant enzymes have an important role in plant responses and defence mechanisms during this biotic stress and that in some cases there were significant relationships between them. However the levels of these compounds either initially or after pathogen inoculation, could not explain the degree of tolerance to powdery mildew in the four wheat lines investigated.  相似文献   

Immunocytochemical and ultrastructural identification of adenohypophyseal cells in Ctenopharyngodon idella (Cypriniformes: Cyprinidae) during gonadal differentiation     

Gilberto Grandi  Maria Gabriella Marchetti  Mattia Lanzoni  Milvia Chicca 《Fish physiology and biochemistry》2014,40(4):1115-1139

The adenohypophysis was studied by immunocytochemical and ultrastructural methods in juvenile grass carp (Ctenopharyngodon idella) from natural reproduction in Northern Italian rivers. The adenohypophysis included the rostral pars distalis (RPD), the proximal pars distalis (PPD) and the pars intermedia (PI), all deeply penetrated by branches of the neurohypophysis (Nh). The prolactin (PRL), adrenocorticotropic (ACTH), somatotropic (GH), thyrotropic (TSH), gonadotropic type I (GtH I) and type II (GtH II), somatolactin (SL), melanotropic (MSH) and endorphin (END) cells were identified with antisera raised against piscine and human pituitary hormones. In juveniles of 51–69 mm of total body length (TL) with undifferentiated gonads, the PRL cells, arranged in thick strands, occupied most of the RPD. The ACTH and GH cells organized in cords bordering Nh were, respectively, confined to RPD and PPD. The TSH cells were scattered among ACTH cells in RPD and among GH cells in PPD. Cells simultaneously immunoreactive to anti-follicle stimulating hormone and to anti-croaker gonadotropin were intermingled among GH and TSH cells, which were mostly in the dorsal PPD. The SL cells were detected in PI layers bordering the Nh. The MSH and END cells were intermingled in PI and, unlike what observed in other teleosts, their respective antisera did not cross-react. In individuals of 78–112 mm TL with gonads at the beginning of differentiation, the GtH II cells were detected in PPD; all other cell types increased in number. These results, supported by ultrastructural investigations, suggest that SL and GtH II cells are directly involved in gonadal differentiation in C. idella.  相似文献