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香菇多糖提取纯化研究 总被引:8,自引:0,他引:8
用0.2mol/L三氯乙酸、0.2mol/L氢氧化钠、1.0mol/L乙酸、蒸馏水为提取溶剂,结合超声波处理等方法从香菇子实体中提取香菇多糖,提取液经甲醇除杂、沉淀处理得粗多糖。粗多糖用CTAB络合沉淀,沉淀物乙酸溶解后取上清再用甲醇沉淀,沉淀物冻干得纯化多糖。分析检测结果显示干香菇三氯乙酸提取的纯化多糖的含量高达93.1%、提取得率达0.324%。薄层分析表明,纯化香菇多糖由葡萄糖、甘露糖、半乳糖、木糖组成。纯化多糖用Sephadex G-100凝胶层析,洗脱液经苯酚-硫酸法检测为单一吸收峰,红外光谱显示其具有多糖类的特征吸收峰。 相似文献
955.
Lin Shanzhi Zhang Zhiyi Lin Yuanzhen Liu Wenfeng Guo Huan Zhang Wei Zhang Chong 《中国林学(英文版)》2004,6(3):1-8
To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H2O2, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the
ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P. suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate
reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced
glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal
ones during the whole culture period. While in comparison with normal shoots, the higher activity of superoxide dismutase
(SOD) and the more concentrations of water, H2O2, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates
that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione
cycle might be closely correlated to the accumulation of H2O2. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots
might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH.
The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could
result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase
in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione
pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H2O2 and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying
shoots of P. suaveolens in tissue culture.
[Supported by National Natural Science Foundation of China (Grant No. 30271093) and the Foundation of State-designated Base
for Biology Researching and Teaching in Beijing Forestry University] 相似文献
956.
对以云南松破壁松花粉为原料 ,采用具创新性生物技术研制的云南松花粉脂提物精粉、活性酶精粉、黄酮精粉、营养及活性物质精粉 ,以及用此 4种精粉经加工而获得的亚油酸软胶囊、口服 0 #胶囊、黄酮药片 (胶囊 )、精粉口服液和松花粉酮酒 5种产品的成分进行了分析 ,了解到它们相关成分的含量。分析结果表明 ,4种云南松花粉精粉具有速溶性好、营养及生物活性物质集中的特点 ,其主要营养及生物活性物质含量数倍于破壁松花粉。以其为原料加工的各项产品很好地保留了精粉的主要功能成分 ,因而对人体具有更高的保健、医疗功效。 相似文献
957.
Guo Jianfen Xie Jinsheng Lu Haoliang Liu Dongxia Yang Yusheng Chen Guangshui 《中国林学(英文版)》2004,6(1):33-36
The amount of carbon returned through litterfall and its seasonal pattern were studied in a natural forest of Castanopsis kawakamii (NF) and adjacent monoculture plantations of C. kawakamii (CK) and Chinese fir (Cunninghamia lanceolata) (CF) in Sanming, Fujian Province, China. Mean annual carbon return through total litterfall over 3 years (from 1999 to 2001)
was 5.097 t·hm−2 in the NF, 4.337 t·hm−2 in the CK and 2.502 t·hm−2 in the CF respectively. Of the total carbon return in the three forests, leaf contribution accounted for 58.96%, 68.53% and
56.12% and twig 24.41%, 22.34% and 26.18%, respectively. The seasonal patterns of carbon return from total litterfall and
leaf-litter were quite similar among the three forests. A peak of carbon input from litterfall in the NF and the CK occurred
in spring except for the highest annual C return through branch litter of the NF in summer, while the CF showed the maximum
C return in summer. The results of this study demonstrate that the natural forest has a greater C return through litterfall
than monoculture plantations, which is beneficial to the increase of soil organic matter storage and the maintenance of soil
fertility.
[Supported by the Teaching and Research Award Program of MOE P.R.C. (TRAPOYT) and the Key Basic Research Project of Fujian
Province (2000F004)] 相似文献
958.
温度对大叶桃花心木(Swietenia macrophylla King)幼苗叶片的光合影响 总被引:2,自引:0,他引:2
本文研究了大叶桃花心木(Swietenia macrophylla King)一年生幼苗在经过夜温处理后的光响应曲线和在饱和光强下的CO2反应曲线.结果表明:在大气CO2浓度下,叶片的最佳光合作用温度在25-31℃之间,而在饱和CO2浓度下为31-35℃.在25℃以下光合速率开始降低,主要是由于羧化效率的降低,而当温度超过31℃时,光合速率下降,是因为羧化效率的降低和呼吸速率的增加.CO2浓度对光合的促进作用在低温下受到抑制,这意味着未来在CO2浓度增高的情况下,高浓度的CO2对热带常绿植物光合的促进在冬天低温情况下表现不十分明显.图4参23. 相似文献
959.
960.