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61.
The Muhammad Nur Reservoir (MNR) is a major multipurpose reservoir located on the Riam Kanan River in the Indonesian Province of South Kalimantan. The MNR has a high sediment trap efficiency and there is concern that accelerated erosion in the catchment, resulting from inappropriate land use, poses a significant threat to the storage capacity and effective life span of the reservoir. Furthermore, on-going deforestation of large parts of the catchment may have changed the inflow pattern to the reservoir. This paper uses data from fieldwork and published information from different sources to examine the extent of erosion and sedimentation in the catchment and the sediment delivery to the reservoir. Water level variations in the reservoir over a 22-year period are examined for changes that might indicate an alteration of the hydrologic balance in the catchment.  相似文献   
62.
Concern about acidification in upland areas has brought about the need to model the stream hydrochemical response to deposition and land-use changes and calculate critical loads. Application of dynamic models such as MAGIC are preferable to steady-state methods, since they are able to produce an estimate of the time scale required to meet some water chemistry target given a reduction in acid deposition. These models typically consider annual changes in stream chemistry at one point. However, in order to protect biota from 'acid episodes', quantification of temporal variability needs to encompass event responses; in addition spatial variability across the catchment also needs to be considered. In this paper, modelling of both spatial and temporal variability is combined in a new framework which enables quantification of catchment hydrochemical variability in time and space. Both low and high flow hydro-chemical variability are quantified in terms of statistical distributions of ANC (Acid Neutralisation Capacity). These are then input as stochastic variables to an EMMA (End-Member Mixing Analysis) model which accounts for temporal variability and ANC is hence predicted as a function of time and space across the whole catchment using Monte-Carlo simulation. The method is linked to MAGIC to predict future scenarios and may be used by iteration to calculate critical loads. The model is applied to the headwaters of the River Severn at Plynlimon, Wales, to demonstrate its capabilities.  相似文献   
63.
Three models, viz., areal non-point source watershed environment response simulation (ANSWERS), universal soil loss equation (USLE) and adapted universal soil loss equation (AUSLE) are evaluated for their performance under the field conditions of the Riam Kanan catchment in South Kalimantan province of Indonesia. While ANSWERS is evaluated for its accuracy to predict both runoff and soil loss, USLE and AUSLE are evaluated for soil loss only. The study was carried out in the context of sedimentation concerns for the Muhammad Nur Reservoir—an important source of drinking and irrigation water supply for the catchment. The models are evaluated using field data collected under four different land uses and during 2 years of field experiments. The land uses considered are cropland with minimum tillage, cropland with conventional tillage, grassland and areas reforested with rubber trees. The ANSWERS model in general has a tendency to overpredict runoff values. The ANSWERS model also was relatively better for predicting soil loss followed by the AUSLE and USLE models. Overall, the ANSWERS model proved superior for predicting soil loss in the Riam Kanan catchment. However, given that the AUSLE model produced sufficiently reliable results and is relatively easy to use, the AUSLE model would also appear to be a useful tool for predicting soil erosion in the catchment.  相似文献   
64.
65.
Specific tropism of HIV-1 for microglial cells in primary human brain cultures   总被引:34,自引:0,他引:34  
Human immunodeficiency virus (HIV) frequently causes neurological dysfunction and is abundantly expressed in the central nervous system (CNS) of acquired immunodeficiency syndrome (AIDS) patients with HIV encephalitis or myelopathy. The virus is found mostly in cells of the monocyte-macrophage lineage within the CNS, but the possibility of infection of other glial cells has been raised. Therefore, the effects of different HIV-1 and HIV-2 strains were studied in primary cultures of adult human brain containing microglial cells, the resident CNS macrophages, and astrocytes. These cultures could be productively infected with macrophage-adapted HIV-1 isolates but not with T lymphocyte-adapted HIV-1 isolates or two HIV-2 isolates. As determined with a triple-label procedure, primary astrocytes did not express HIV gag antigens and remained normal throughout the 3-week course of infection. In contrast, virus replicated in neighboring microglial cells, often leading to their cell fusion and death. The death of microglial cells, which normally serve immune functions in the CNS, may be a key factor in the pathogenesis of AIDS encephalitis or myelopathy.  相似文献   
66.
Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and in the carotid body it is crucial to respiratory control. However, the identity of the oxygen sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) is part of the BK channel complex and enhances channel activity in normoxia. Knockdown of HO-2 expression reduced channel activity, and carbon monoxide, a product of HO-2 activity, rescued this loss of function. Inhibition of BK channels by hypoxia was dependent on HO-2 expression and was augmented by HO-2 stimulation. Furthermore, carotid body cells demonstrated HO-2-dependent hypoxic BK channel inhibition, which indicates that HO-2 is an oxygen sensor that controls channel activity during oxygen deprivation.  相似文献   
67.
We have isolated a compound responsible for the cytokinin activity of soluble RNA from Escherichia coli. The structure, indicated as 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine, C(16)H(23)N(5)0(4)S, on the basis of low-and high-reso!ution mass spectrometry, was established by unequivocal synthesis. The mass spectra, chromatographic behavior, and ultraviolet spectra of the compounds from natural and synthetic sources were identical.  相似文献   
68.
69.
Voyager 1 (V1) began measuring precursor energetic ions and electrons from the heliospheric termination shock (TS) in July 2002. During the ensuing 2.5 years, average particle intensities rose as V1 penetrated deeper into the energetic particle foreshock of the TS. Throughout 2004, V1 observed even larger, fluctuating intensities of ions from 40 kiloelectron volts (keV) to >/=50 megaelectron volts per nucleon and of electrons from >26 keV to >/=350 keV. On day 350 of 2004 (2004/350), V1 observed an intensity spike of ions and electrons that was followed by a sustained factor of 10 increase at the lowest energies and lesser increases at higher energies, larger than any intensities since V1 was at 15 astronomical units in 1982. The estimated solar wind radial flow speed was positive (outward) at approximately +100 kilometers per second (km s(-1)) from 2004/352 until 2005/018, when the radial flows became predominantly negative (sunward) and fluctuated between approximately -50 and 0 km s(-1) until about 2005/110; they then became more positive, with recent values (2005/179) of approximately +50 km s(-1). The energetic proton spectrum averaged over the postshock period is apparently dominated by strongly heated interstellar pickup ions. We interpret these observations as evidence that V1 was crossed by the TS on 2004/351 (during a tracking gap) at 94.0 astronomical units, evidently as the shock was moving radially inward in response to decreasing solar wind ram pressure, and that V1 has remained in the heliosheath until at least mid-2005.  相似文献   
70.
Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.  相似文献   
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