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Soils from field sites at Foulum (DK), Narbons (FR) and Varois (FR) planted with genetically modified maize expressing either the insecticidal Bacillus thuringiensis protein (Bt) or herbicide tolerance (HT), as described elsewhere in this volume, were analysed for nematodes, protozoa and microbial community structure. These analyses were mirrored in single-species testing and in mesocosm experiments, and were coordinated with field samples taken for microarthropods, enchytraeids and earthworms so allowing for cross-comparison and a better understanding of the results observed in the field. Over the first 2 years of the field experiments (in 2002 and 2003), the effect of Bt-maize was within the normal variation expected in these agricultural systems. Sampling in 2004 and 2005 was expanded to include the effects of tillage (i.e. reduced tillage versus conventional tillage) and also the use of HT-maize. Tillage had major effects regardless of soil type (Varois or Foulum), with reduced-tillage plots having a greater abundance of microfauna and a different microbial community structure (measured both by phospholipid fatty-acid analysis (PLFA) and by community-level physiological profiling (CLPP)) from conventionally tilled plots. Grass, as a contrasting cropping system to maize, also had an effect regardless of soil type and resulted in greater microfaunal abundance and an altered microbial community structure. Differences in crop management, which for the Bt-maize was removal of the insecticide used to control European corn borer and for HT-maize was a change in herbicide formulation, were only tested at single sites. There were differences in microbial community structure (CLPP but not PLFA) and sporadic increases in protozoan abundance under the Bt-crop management. The HT-maize cropping system, which covered a shorter period and only one site, showed little change from the conventional system other than an altered microbial community structure (as measured by PLFA only) at the final harvest. The Bt-trait had a minimal impact, with fewer amoebae at Foulum in May 2003, fewer nematodes at Foulum in May 2004 but more protozoa at Varois in October 2002 and an altered microbial community structure (PLFA) at Foulum in August 2005. These were not persistent effects and could not be distinguished from varietal effects. Based on the field evaluations of microfauna and microorganisms, we conclude that there were no soil ecological consequences for these communities associated with the use of Bt- or HT-maize in place of conventional varieties. Other land management options, such as tillage, crop type and pest management regime, had significantly larger effects on the biology of the soil than the type of maize grown.  相似文献   
13.
Soil variability and pesticide behaviour The spatial variability of selected soil characteristics (organic carbon, particle size), pesticide adsorption and leaching was estimated for an agricultural location. Variograms were calculated to describe the spatial dependence of data. All parameters showed a nearly isotropic behaviour. According to variograms a kriging interpolation was done and the spatial variability of parameters was estimated. Leaching of terbuthylazine was calculated (model PRZM) with regard to soil variability and adsorption pattern (Kd-values). Leaching depth in the winter season varied between 10 and 22 cm depending strictly on soil characteristics.  相似文献   
14.
Reconstituted skim milk was adjusted to pH values between 6.5 and 7.1 and heated (90 degrees C) for up to 30 min. The skim milk samples were then readjusted to pH 6.7. Acid gels prepared from heated milk had markedly higher G ' values, a reduced gelation time, and an increased gelation pH than those prepared from unheated milk. An increased pH at heating decreased the gelation time, increased the gelation pH, and increased the final G ' of acid set gels prepared from the heated milk samples. There were only small differences in the level of whey protein denaturation in the samples at different pH values, and these differences could not account for the differences in the G ' of the acid gels. The levels of denatured whey protein associated with the casein micelles decreased and the levels of soluble denatured whey proteins increased as the pH at heating was increased. The results indicated that the soluble denatured whey proteins had a greater effect on the final G ' of the acid gels than the denatured whey proteins associated with the casein micelles.  相似文献   
15.
Patchy weed distribution and site-specific weed control in winter cereals   总被引:1,自引:2,他引:1  
Site-specific weed control in winter cereals was performed on the same fields every year over a 5-year period (1999–2003). The most common weeds (Apera spica-venti, Galium aparine, Veronica hederifolia, Viola arvensis) were counted by species, at grid points which were georeferenced and the data were analysed spatially. For weed control, weeds were grouped into three classes: grass, broad-leaved weeds (without Galium aparine), and Galium aparine. Based on weed distribution maps generated by the spatial analyses, herbicide application maps were created and site-specific herbicide application was carried out for grouped and or single weed species. This resulted in a significant reduction in herbicide use. Averaging the results for all fields and years, the total field area treated with herbicides was 39% for grass weeds, 44% for broad-leaved weeds (without Galium aparine) and 49% for Galium aparine. Therefore, site-specific weed control has the potential to reduce herbicide use compared to broadcast application, thus giving environmental and economic benefits.  相似文献   
16.
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.  相似文献   
17.
Until1887pig breeding in Denmark wasbased upon the export to Germany of heavy pigs,weighing from120to150kg.To satisfy thismarket the old native Danish Landrace breed wascrossed with Middle White and Berkshire boarsimported from England in order to produce stillfatter pigs.In1887the importation of Danishpigs became prohibited by Germany and ourfarmers had to change over to the production ofbacon for the English market.This change over was not an easy one.Forthe production of bacon,a comple…  相似文献   
18.
After fruits, roots, or the mycelium of certain plants were treated with 2-(4-chlorophenylthio)-triethylamine hydrochloride, lycopene was detected in the tissue. This is the first known success in causing lycopene to accumulate in a wide range of carotenogenic tissues that normally do not accumulate the pigment at some stage of development. The response should be of value in the study of carotenoid biosynthetic pathways and gene control mechanisms.  相似文献   
19.
Rain water and dustfall deposition samples were collected at Pune, an urban site (1992–98) and at Sinhagad, a rural site (1992–94). The samples were collected with wet-only and bulk collectors at Pune and with bulk collector at Sinhagad. The samples were analyzed for major ions, pH and conductivity. The study showed that the rain water at both places is alkaline (pH > 5.6). The average pH at Pune was 6.1. Neutralising components, indicated by Ca and non sea salt (nss) Mg have higher concentrations than the acidifying components SO4 and NO3. The wet deposition fluxes of all the ionic components were higher than the dustfall fluxes. Relative contribution from dustfall was largest for K, Ca, Mg and NO3. Dustfall was greater at Pune, compared to Sinhagad for all components and up to double for Ca.  相似文献   
20.
The question was examined under which conditions the water extraction rate of plant roots in the field can be limited by water transport to the roots. For this purpose we used a numerical solution of the single root model. Scenario calculations were carried out in order to investigate the general model behaviour. A sensitivity analysis showed that initial volumetric water content and root length density are of greater importance than root diameter in determining the maximum water transport rate to the roots. Data from a field experiment were taken, describing root length density, volumetric water content and water uptake rates under oats (Avena saliva L.) and faba beans (Vicia faba L.) as model input parameters. With this data the model calculated the water content difference between the bulk soil and the root surface which is necessary to induce a water flow to the roots matching the observed water uptake rate. Root length densities below the grain legume crop faba beans are one order of magnitude lower compared to that of the cereal crop oats. The therefore higher specific water influx rates of faba beans roots resulted in a higher decrease in water content near the root surface. However, water uptake by faba beans was controlled by the water flow towards the roots probably only in deeper soil layers with very low root length density. For the given conditions no transport limitation of water uptake was calculated, when rooting densities were higher than about 0.1 cm.cm?3.  相似文献   
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