Effects of coffee bean aroma on the rat brain stressed by sleep deprivation: a selected transcript- and 2D gel-based proteome analysis     

Seo HS  Hirano M  Shibato J  Rakwal R  Hwang IK  Masuo Y 《Journal of agricultural and food chemistry》2008,56(12):4665-4673

  相似文献   

Water absorption of boron-treated and heat-modified wood     

Saip Nami Kartal  Won-Joung Hwang  Yuji Imamura 《Journal of Wood Science》2007,53(5):454-457

Heat treatments change the chemical and physical properties of wood and dimensional stability and hygroscopicity are affected as a result of modifications of wood cell components. This study evaluated the water absorption of wood specimens treated with boron compounds followed by heat treatment. Sugi (Cryptomeria japonica D. Don) sapwood specimens treated with either boric acid (BA) or disodium octoborate tetrahydrate (DOT) solutions were heat-modified at either 180° or 220°C for 2 or 4 h. Carbohydrate composition and water absorption of the specimens were then measured and compared with those of untreated and unheated specimens. Wood carbohydrates were significantly degraded in the specimens after heat treatment. The heat treatment evidently decreased the water absorption and the heat-modified specimens absorbed less water than unheated specimens. The higher the treatment temperature and the longer the treatment time, the lower the amount of absorbed water. The boron-treated and heat-modified specimens, however, showed increased water absorption due to the hygroscopic properties of BA and DOT.  相似文献   

Directly-prelithiated carbon nanotube film for high-performance flexible lithium-ion battery electrodes     

Sehyun Lee  Hyeonjun Song  Jun Yeon Hwang  Youngjin Jeong 《Fibers and Polymers》2017,18(12):2334-2341

Carbon nanotube (CNT) films are very flexible and serve as active materials for lithium-ion batteries (LIBs). Hence, they have high potential as flexible free-standing electrodes for wearable batteries. However, nanocarbon materials such as CNTs and graphene are of limited use as electrodes because they have a large initial irreversible capacity due to the formation of a solid electrolyte interphase (SEI). Herein, we prelithiated the CNT films to make them available as electrodes for flexible batteries by reducing their irreversible capacity. The SEI is pre-formed through a direct prelithiation (DP) method that brings lithium metal into direct contact with CNT films in an electrolyte. As a result, the capacity of directly-prelithiated CNT film electrodes continues to increase to 1520 mAh/g until 350th cycle of charge/discharge and their initial irreversible capacity vanishes. The changes in the electrochemical properties of CNT film electrodes by DP treatment and their flexibility are investigated.  相似文献   

Use of monoclonal antibodies to identify outer membrane antigens of Actinobacillus species     

M C Healey  H M Gharpure  S J Kleinschuster  H H Hwang  A V Johnston 《American journal of veterinary research》1986,47(7):1446-1451

Three monoclonal antibodies (LG17, LG30, and LG33) were used to identify outer membrane antigens of Actinobacillus sp (As8C isolate) cultured from the epididymides of an infected ram lamb. Specificity of the 3 antibodies to As8C antigens was determined by use of bacterial agglutination, the enzyme-linked immunosorbent assay, and the indirect fluorescent antibody test. Results of immunoelectron microscopy confirmed that each antibody was specific for epitopes on As8C outer membrane antigens. Evaluation by use of enzyme-linked immunoelectrotransfer blot indicated that target antigens for LG17 and LG33 antibodies had molecular weights of 10 kilodaltons and 43 kilodaltons, respectively. Multiple-band staining was observed with the LG33 antibody. The target antigen for the LG30 antibody could not be discerned by use of enzyme-linked immunoelectrotransfer blot. For each of the 3 monoclonal antibodies, enzyme-linked immunosorbent assay titers were obtained for Actinobacillus seminis, A actinomycetemcomitans, and 10 field isolates of Actinobacillus spp. Target antigens for LG17 and LG30 antibodies occurred infrequently or were absent on these bacteria. However, the target antigen for the LG33 antibody was shared by Actinobacillus seminis, A actinomycetemcomitans, and the 10 field isolates of Actinobacillus spp, indicating some diversity of outer membrane antigens between isolates.  相似文献   

Improved monospermic fertilization and subsequent blastocyst formation of bovine oocytes fertilized in vitro in a medium containing NaCl of decreased concentration     

Roh S  Hwang W  Lee B  Lim J  Lee E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):667-671

The objective of this study was to evaluate whether changes in NaCl concentration in a fertilization medium could improve normal fertilization and preimplantation development of bovine oocytes. In vitro matured bovine oocytes were inseminated with frozen-thawed semen for 18 hr in a Tyrode's medium with albumin, lactate and pyruvate (TALP), to which 114 (TALP-114), 96 (TALP-96) or 78 (TALP-78) mM NaCl was added. Presumptive zygotes were cultured for 192 hr in a modified TALP containing 90 mM NaCl, 1.5 mM glucose, 0.3% (w/v) BSA, minimal essential medium (MEM) essential and nonessential amino acids, and insulin-transferrin-selenium complex. Lower polyspermy rate was obtained by the insemination in TALP-96 (7.8 +/- 2.3%) than by the insemination in TALP-114 (25.6 +/- 1.4%), without decrease in male pronucleus (MPN) formation. Fertilization in TALP-78 also yielded decreased polyspermic fertilization (3.8 +/- 1.5%), but significant decrease in MPN formation was found (63.1 +/- 3.1%). In preimplantation development, more blastocysts developed from oocytes inseminated in TALP-96 (24.1 +/- 1.7%) than from oocytes inseminated in TALP-114 (16.8 +/- 1.4%). TALP-78, however, did not improve preimplantation development beyond the 8-cell stage compared with TALP-114. Mean cell number of blastocyst was higher when oocytes were fertilized in TALP-96 (137.0 +/- 4.5) than in TALP-114 (123.1 +/- 5.1) and in TALP-78 (102.3 +/- 4.5). These results demonstrate that insemination of bovine oocytes in a TALP with decreased NaCl concentration (96 mM) improves blastocyst formation and embryo viability. Decrease in NaCl concentration below 96 mM, however, may delay or inhibit MPN formation, and inhibits subsequent development in vitro.  相似文献   

Encephalomyelitis associated with akabane virus infection in adult cows   总被引：8，自引：0，他引：8  

Lee JK  Park JS  Choi JH  Park BK  Lee BC  Hwang WS  Kim JH  Jean YH  Haritani M  Yoo HS  Kim DY 《Veterinary pathology》2002,39(2):269-273

  相似文献   

In vitro isolation and characterization of bovine Neospora caninum in Korea   总被引：5，自引：0，他引：5  

Kim JH  Sohn HJ  Hwang WS  Hwang EK  Jean YH  Yamane I  Kim DY 《Veterinary parasitology》2000,90(1-2):147-154

The brains of nine aborted bovine fetuses and two newborn calves born from dams suspected to be infected with Neospora caninum were homogenized and inoculated into Vero cells. All fetuses and calves were from cows determined as seropositive to N. caninum by an IFA test. Sera and thoracic fluids of all fetuses and calves also revealed high antibody titer to N. caninum by IFAT ranging from 1:800 to 1:3200. N. caninum was isolated from the brains of one aborted fetus and one newborn calf when the brain homogenates were grown continuously in Vero cell culture. N. caninum tachyzoites, giemsa-positive, were first observed on Days 45 and 56 postinoculation in the newborn calf and the aborted fetus, respectively. The isolates (KBA-1 and KBA-2) were morphologically and ultrastructurally similar to previously published Neospora isolates. The isolated parasites were confirmed as N. caninum by means of the antigenic reactivities, immunostaining, PCR and southern blotting, and electron microscopy.  相似文献   

Effects of autogenous toxoid-bacterin in lactating cows with Staphylococcus aureus subclinical mastitis     

Hwang CY  Pak SI  Han HR 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(8):875-880

To evaluate clinical effects of autogenous toxoid-bacterin treatment for Staphylococcus aureus subclinical mastitis in lactating cows, 22 cows which had at least one S. aureus infected quarter were selected from among cows at a S. aureus prevalent dairy farm. Eleven cows were injected with their own autogenous toxoid-bacterin and the others were maintained as non-injected control. In the toxoid-bacterin injected group, 27% of infected quarters were cured during the 12-week trial, compared to 5% in the control group. New intramammary infections with S. aureus were only detected in 3 quarters of the control group. Mean IgG antibody titer against S. aureus somatic antigens and alpha-toxin in serum and milk were significantly increased in the toxoid-bacterin injected group (p<0.05) and remained higher than those of the control group which showed no significant changes (p<0.05). In contrast to the control group, from 3 weeks after the second injection of the toxoid-bacterin injected group, mean S. aureus cfu/ml in milk samples from injected quarters with S. aureus was significantly decreased until the end of the study (p<0.05). In the toxoid-bacterin injected group, significant decreases of mean SCC were detected from milk samples from infected quarters with S. aureus from week 7 to week 10 (p<0.05). These data show that autogenous toxoid-bacterin treatment against S. aureus subclinical mastitis in lactating cows may increase the cure rate of the infections, reduce the severity of the infections and also prevent occurrence of the new infections.  相似文献   

Microscopic structure features changes of rice straw treated by boiled acid solution     

C.T. Yu  W.H. Chen  L.C. Men  W.S. Hwang 《Industrial Crops and Products》2009,29(2-3):308-315

This study focuses on the determination of structure change of surface area, morphology, crystallinity and pyrolysis characteristics of cellulosic feedstock treated by hot acid solution. The microscopic structure change of acid-treated samples was examined by using BET, SEM/EDX, XRD and pyrolysis–GC–MS under reasonable condition. Results showed that both surface area and pore volume of treated sample were conspicuous increased partial due to dissolution of silica and organic polar-components. Morphological change of plant cell wall surface was viewed as damage such as exfoliation, cave and fracture appeared on epidermis, vascular bundles. The crystallinity index of residual cellulose microcrystalline was widely enhanced because amorphous hemicellulose was removed off while crystalline was changeless. Under hot acid solution reaction, amorphous hemicellulose was hydrolyzed and leaving larger perfect cellulose thus increasing of crystalline size. Those available cellulose was obvious exposed after acid treatment and gave intensive characteristic peak on pyrogram analyzed by pyrolysis–GC–MS.  相似文献   

Enhancement of oxidative response and cytokine production by yam mucopolysaccharide in murine peritoneal macrophage   总被引：1，自引：0，他引：1  

Choi EM  Hwang JK 《Fitoterapia》2002,73(7-8):629-637

Yam (Dioscorea batatas) mucopolysaccharide (YMP) was examined for the ability to induce secretory and cellular responses in murine peritoneal macrophages. When macrophages were treated with various doses (10-100 microg/ml) of YMP for 48 h, a significant cytotoxicity against B 16 cells was observed. YMP also increased the myeloperoxidase activity and the production of H(2)O(2), O(2)(-), nitric oxide (NO) and tumor necrosis factor (TNF-alpha). However, there was a little alteration on the production of IFN-gamma. These results indicate that reactive oxygen intermediates (ROI), reactive nitrogen intermediates (RNI) and TNF-alpha are likely major mediators of cytotoxic activity of YMP-treated macrophages.  相似文献