Localization of inhibin alpha-, betaA- and betaB-subunits and aromatase in ovarian follicles with granulosa theca cell tumor (GTCT) in 6 mares     

Hoque S  Derar RI  Senba H  Osawa T  Kano K  Taya K  Miyake Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):713-717

To clarify the morphological and immunohistochemical characteristics in mares with granulosa theca cell tumor (GTCT), the localization of inhibin subunits (alpha, betaA, betaB) and aromatase in the granulosa cell layers and theca layers in the ovarian follicles were determined by immunohistochemical staining. The follicles were obtained from the ovaries of 6 mares with GTCT and 4 normal mares as controls. Immunohistochemically, inhibin alpha-subunit was localized in the granulosa cells of all follicles showing different sizes in all GTCT cases and betaA- subunit was localized in two GTCT cases in all sized follicles. But inhibin betaB- subunit and aromatase were not localized in GTCT cases. On the other hand, inhibin alpha-, betaA-, and betaB-subunits and aromatase were localized in the large and medium sized follicles, but inhibin betaA- and betaB-subunits and aromatase were not stained in the small sized follicles in normal cases. These findings suggest that some mares with GTCT can secrete dimeric inhibin (inhibin A), but all GTCT cases cannot secrete inhibin B. By the results of aromatase staining it is clear that testosterone is not converted into estradiol due to the lack of aromatase in the GTCT follicles.  相似文献   

Reduction and stabilization of hip luxation by the transposition of the ligamentum sacrotuberale in dogs: an in vivo study     

Ozaydin I  Kiliç E  Baran V  Demirkan I  Kamiloglu A  Vural S 《Veterinary surgery : VS》2003,32(1):46-51

OBJECTIVES: To evaluate the effectiveness of transposition of the sacrotuberous ligament (LST) for the treatment of coxofemoral luxation in the dog. STUDY DESIGN: In vivo experimental study. SAMPLE POPULATION: Ten mixed-breed dogs (weighing 12 to 26 kg). METHODS: After general anesthesia, the LST was exposed and released from the sacrum with a sacral bone fragment. Coxofemoral luxation was created by capsulotomy and transection of the ligament of the head of the femur. Suture was passed through 2 small holes in the bone fragment to guide transposition of the LST through a tunnel drilled through the acetabulum and femoral head and neck. The suture material was pulled tight and an interference screw was placed into the femoral tunnel to lock the sacral bone fragment and LST securely in the tunnel. Butorphanol was administered for pain relief and dogs were allowed unrestricted activity. Coxofemoral radiographs were obtained at 15-day intervals. Two dogs were euthanatized for macroscopic and histopathologic examinations at 3 months. RESULTS: Release of the sacral bone was moderately difficult. The transposed ligament and bone fragment were of an appropriate length and were easily and securely fixed by a interference screw method. Visible severe lameness, during the first 7 to 10 days improved to mild lameness within 10 to 21 days. Gait was subjectively normal after 5 weeks. No radiographic abnormalities were observed at any time point. Grossly, the LST was intact and apparently viable and functional. On histopathology, the space in the bone tunnel was filled with new tissue and a hyaline-like layer surrounded the LST. The sacral bone fragment attached to the LST was united with the femoral bone. CONCLUSIONS: Transposition of the LST reduced and stabilized experimentally induced coxofemoral luxation in 10 dogs. Survival of the LST up to 3 months and its adaptation to transposition suggest that this surgical technique could be considered as a permanent treatment option for hip luxations. Transposition and fixation of the LST was easy, but releasing the sacral edge of the LST was difficult because of its deep location and division of the origin into 2 branches in some dogs. CLINICAL RELEVANCE: Results of this experimental study suggest that the technique may be satisfactorily used in dogs with coxofemoral luxation.  相似文献   

The detection of the meq gene in chicken infected with Marek's disease virus serotype 1   总被引：4，自引：0，他引：4  

Chang KS  Lee SI  Ohashi K  Ibrahim A  Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(5):413-417

In the genome of strains of very virulent Marek's disease virus serotype 1(vvMDV1), such as Md5 and RB1B, the meq open reading frame (ORF) encoding a 339-amino-acid bZIP protein, is present, while a slightly longer meq ORF, termed as L-meq, in which a 180-bp sequence is inserted into the meq ORF is found in other strains of MDV1, such as CV1988/R6 and attenuated JM. When chickens were infected with vvMDV1 strains and the meq gene was amplified by nested polymerase chain reaction (PCR), the meq gene was detected throughout the experimental period for 7 weeks post inoculation (pi). However, the L-meq gene was also detected at 3 to 5 weeks and 3 to 4 weeks pi. in Md5-infected and RB1B-infected chickens, respectively. In the case of chickens infected with an attenuated MDV1, the JM strain, the L-meq gene was detected at 2 to 7 weeks pi., and the meq gene was also detected at 2 to 6 weeks pi. Both L-meq and meq genes were detected in chickens infected with an attenuated nononcogenic vaccine strain of MDV1 (CVI988/R6), throughout the experimental period. Though quantitative PCR was not performed, a larger amount of the PCR products corresponding to the L-meq than the meq gene was amplified from chickens infected with JM or CVI988/R6. These results suggest that a dynamic population shift between the MDV subpopulations displaying meq and L-meq genes occurs in chickens during the course of MDV infection. Since the MDV subpopulation that displays the L-meq gene only displays it during the latent phase, the L-meq and its gene product, if any, might contribute to the maintenance of the MDV latency.  相似文献   

Ameliorative effects of sodium bentonite on phagocytosis and Newcastle disease antibody formation in broiler chickens during aflatoxicosis   总被引：1，自引：0，他引：1  

Ibrahim IK  Shareef AM  Al-Joubory KM 《Research in veterinary science》2000,69(2):119-122

The ameliorative effect of graded levels of dietary sodium bentonite (0.2, 0.4 and 0.6 per cent wt/wt of feed) on in vitro-impaired phagocytosis and suppressed immune response to Newcastle disease vaccine during aflatoxicosis (AF) in broiler chicks was investigated. Both percentage and mean of phagocytic activities were decreased significantly (P < 0.05) in chicks fed 2.5 mg aflatoxin per kg feed. The addition of sodium bentonite was significantly effective in ameliorating the negative effect of AF on the percentage and mean of phagocytosis. The presence of AF alone in the diet depressed the immune response of chicks as measured by haemagglutination inhibition (HI) test. Sodium bentonite was also effective in ameliorating the suppressive effect of AF on the HI -titre in chicks vaccinated against Newcastle disease. The best results obtained when sodium bentonite was added at the rate of 0.4 per cent wt/wt of feed to the AF-containing diets.  相似文献   

Effect of temperature and photoperiod on development and fecundity of an acarophagous ladybird beetle,Stethorus gilvifrons     

Tulin Aksit  Ibrahim Cakmak  Gamze Ozer 《Phytoparasitica》2007,35(4):357-366

The development, fecundity and survival ofStethorus gilvifrons Mulsant (Coleoptera: Coccinellidae) fed onTetranychus cinnabarinus Boisduval (Acari: Tetranychidae) were recorded at three constant temperatures (20, 25 and 30±1°C) and 50±10% relative humidity, under two photoperiods (16:8 L:D and 8:16 L:D) produced using artificial light (4000 lux). The development rate for the egg stage (r_[Te]) increased linearly with increasing temperature (r_[Te]=0.0132*T ? 0.0955; R²=0.95). The theoretical egg-development threshold was estimated to be 7.24°C; 75.75 degree-days (DD) were required for hatching. The total development time (r_[Tt]) also decreased linearly with increasing temperature (r_[Tt]=0.0039*T ? 0.0325; R²=0.98). The development threshold was estimated to be 8.33°C and full development from egg to adult required 256.41 DD. Higher temperatures resulted in a shorter generation time (T ₀) and decreased net reproductive rate (R ₀). The length of the previposition and postoviposition period, as well as longevity, decreased significantly with increasing temperature under both photoperiods. The oviposition and postoviposition periods, longevity, and total fecundity were not significantly affected by photoperiod. The values of both the intrinsic rate of increase (r _m ) andR ₀ were highest under the long-day photoperiod at 25°C. The mortality rate was lowest at 20°C under the short-day photoperiod. Of the conditions tested, the optimum temperature for rearingS. gilvifrons was 25°C and the optimum photoperiod was 16:8 L:D.  相似文献   

Effect of cultural practices on the development of arabica coffee berry disease,caused by <Emphasis Type="Italic">Colletotrichum kahawae</Emphasis>     

Joseph Aubert Mouen Bedimo  Daniel Bieysse  Ibrahim Njiayouom  Jean Pierre Deumeni  Christian Cilas  Jean Loup Nottéghem 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):391-400

In the high altitude regions of Africa, coffee berry disease (CBD), caused by Colletotrichum kahawae, is the main constraint for arabica coffee (Coffea arabica) production. However, certain agricultural practices can reduce losses caused by the disease and thereby promote optimum production. On small family farms in Cameroon, mixed cropping with fruit trees, intercropping with food crops and maintenance pruning of coffee trees are very widespread agricultural practices that can affect CBD epidemics. Consequently, an epidemiological study was conducted to assess how cultural practices affected the disease in an arabica coffee smallholding in Cameroon. The disease was monitored on a weekly basis over four successive years (2002–2005) on coffee trees in diverse cultural situations. Cultural practices likely to reduce losses due to CBD were identified. The infection rate was significantly lower on coffee trees grown intensively than on coffee trees grown in the traditional manner. Coffee trees located under the shade of fruit trees were significantly less attacked than those located in full sunlight. In addition, berries on the leafless parts of branches, near the main trunk of the coffee tree, were less infected than those on leafy sections. These results show that maintenance pruning, removal of mummified berries, and mixed cropping with shade plants are cultural practices which create environmental conditions that limit CBD development.  相似文献   

A study on bluetongue virus infection in Saudi Arabia using sentinel ruminants     

Abu Elzein EM  Aitchison H  al-Afaleq AI  al-Bashir AM  Ibrahim AO  Housawi FM 《The Onderstepoort journal of veterinary research》1998,65(4):243-251

Four sentinel herds comprising cattle, sheep and goats were established at various localities in Saudi Arabia. Maternal bluetongue antibodies were detected in all four sentinel herds but disappeared in 4-6 months, immediately followed by seroconversion in all. Serological results indicated that the animals were recently exposed to BT virus serotypes 10, 12, 15 and 20. The epidemiology of the disease in Saudi Arabia is discussed.  相似文献   

MicroRNA-21 expression,serum tumor markers,and immunohistochemistry in canine mammary tumors     

Ramadan  Eman S.  Salem  Noha Y.  Emam  Ibrahim A.  AbdElKader  Naglaa A.  Farghali  Haithem A.  Khattab  Marwa S. 《Veterinary research communications》2022,46(2):377-388

Background

Canine mammary tumors (CMTs) are one of the most common malignancies in dogs and are associated with significant mortality. Serum tumor markers and non-coding microRNAs have gained widespread popularity in human oncology studies. The present study has two aims, first one is to investigate the miR-21 expression compared with changes in serum tumor markers (CEA and CA15-3) in CMT. The second aim is to detect the immunohistochemistry markers as vimentin, P63, and -SMA in CMT.

Methods

This study enrolled 17 female dogs: 10 with mammary tumors and seven controls without tumors. Blood samples were collected to measure miR-21, CEA, and CA 15-3, and histological samples were prepared for histological grading and immunohistochemistry.

Results

CA 15-3 was elevated in all animals, whereas CEA levels showed no change compared with controls. miR-21 was upregulated 12.84-fold in animals with CMT. The most frequently recorded CMT was the mixed type. Myoepithelial cells were identified by P63 immunoreactivity, but not SMA. High expression of miR-21 was observed with positive vimentin immunoreactivity, indicating the mesenchymal origin of the tumor cells.

Conclusion

The present study showed that miR-21 was elevated to a greater extent than CA 15-3 (12.84-fold vs. threefold). Tumors that was positive for vimentin immunoreactivity was also associated with an elevation in the levels of miR-21, showing that miR-21 is released from mesenchymal cells. These findings support the hypothesis that miR-21 may be a more sensitive, noninvasive indicator for CMT.

  相似文献   

Genome-wide association studies of preweaning growth and in vivo carcass composition traits in Esme sheep     

Onur Yilmaz  Mehmet Kizilaslan  Yunus Arzik  Sedat Behrem  Nezih Ata  Orhan Karaca  Cengiz Elmaci  Ibrahim Cemal 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):26-39

Sheep are considered as a major contributor of global food security. Moreover, sheep preweaning growth traits as well as in vivo carcass composition traits such as ultrasonic measurements of Longissimus dorsi muscle depth (UMD) and back-fat thickness (UFD) are crucially important indicators of meat yield and hot carcass composition. Despite their relative importance for productivity and profitability of a sheep production system, detected QTL for these traits are quite scarce. Therefore, we implemented GWAS for these traits using animal mixed model-based association approach provided by GenABEL in Esme sheep. Three genome-wide and 14 individual chromosome-wide associated SNPs were discovered. As a result, ESRP1, LOC105613082, ZNF641, DUSP5, TEAD1, SMOX, PTPRT, RALYL, POM121C, PHIP, LOC101106051, ZIM3, PEG3, TRPC7, FBXL4, LOC105610397, LOC105616489 and DNAAF2 were suggested as candidates. Some of the discovered genes and involved pathways were already annotated to contribute growth and development in various species including human, mice and cattle. All in all, the results of this study are expected to strongly contribute to shed a light on the underlying molecular mechanisms behind growth and carcass composition traits, with potential implications on studies aiming faster genetic improvement, targeted low-resolution SNP panel designs and genome-editing studies.  相似文献   

Zur Wirkung von Milchsäure auf den Varroa-Befall der Biene     

Prof. Dr. S. H. Ibrahim  T. H. Ezzat 《Journal of pest science》1993,66(2):31-32

40% lactic acid was sprayed 3 times into each of two bee colonies. The rate of infestation was determined, before beginning the experiment and after each of the applications. The treatments showed a mean percentage reduction in the brood infestation byVarroa of 76,7% in the first colony and 85,2% in the second. These data indicate that it is possible to control the Varroasis by means of lactic acid successfully and without side effects.  相似文献