Switchgrass from North Dakota – an adaptable and promising energy crop for northern regions of Europe     

Nijolė Lemežienė  Eglė Norkevičienė  Giedrė Dabkevičienė  Jurgita Cecevičienė  Bronislava Butkutė 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(2):118-124

Searching for novel energy crops, an interest in C4-type plant switchgrass (SWG) has increased worldwide. The present research was aimed to study SWG genetic resources for most important agrobiological traits with a view of extending the range of energy plants in the Nemoral zone of Europe. SWG was studied for the peculiarities of developmental stages, winter hardiness, and dry matter (DM) yield. SWG regrowth in spring started one month later compared to the reed canary grass (variety ‘Chiefton’). All investigated genotypes matured seeds in the second half of September. The most winter-hardy SWG accessions (0–20% winter damage) were the majority of wild ecotypes and the variety ‘Dacotah’ from North Dakota (2–4 hardiness zone) as well as the variety ‘Summer’ from South Nebraska (4, 5 hardiness zone). Ecotypes from North Dakota exhibited a high breeding potential and prospects in Northern regions of Europe due to genotypic variation of winter hardiness trait. The varieties ‘Alamo’, ‘Falcon’, ‘Grenville’, ‘Shawnee’, and ‘Trailblazer’ that originated from warmer climate zones (6–9 hardiness zone) were heavily damaged or completely killed. The worst overwinter survival of plants was recorded after the first winter. DM yield was estimated at two regimens of cutting. When grass was cut once at seed maturity stage, DM yield in the first harvest year was 249 g per plant, in the second harvest year 349 g per plant, and in the third harvest year 493 g per plant. When grass was cut twice per season (at the beginning of anthesis and after regrowth of aftermath), DM yield was significantly lower: in the first harvest year the DM yield was 203 g per plant (18.4% less), in the second harvest year 182 g per plant (47.9% less), and in the third harvest year 272 g per plant (44.7% less).  相似文献   

Characterization of tomato leaf mould pathogen,Passalora fulva,in Croatia     

Novak  Adrijana  Ćosić  Jasenka  Vrandečić  Karolina  Jurković  Draženka  Plavec  Jelena  Križanac  Ivana  Ivić  Dario 《植物病害和植物保护杂志》2021,128(4):1041-1049

Journal of Plant Diseases and Protection - Leaf mould (Passalora fulva) has emerged as an important disease of greenhouse-growing tomato crops in Croatia during the last decade. In order to...  相似文献   

Phylogenetic analysis of small ruminant lentiviruses detected in Slovenia     

Urška Kuhar  Darja Barlič-Maganja  Jože Grom 《Veterinary microbiology》2013

Small ruminant lentiviruses (SRLV), which belong to the Retroviridae family, infect goats and sheep worldwide. The aim of this study was to characterize the SRLV strains circulating in Slovenia, by phylogenetic analysis of two genomic regions, 1.8 kb gag-pol fragment and 1.2 kb pol fragment. The results of our study revealed that Slovenian SRLV strains are highly heterogeneous, with ovine strains belonging to genotype A and caprine strains to genotypes A and B. The closest relatives of sheep virus sequences from two flocks that clustered together (SLO 35, 36) were found to be in subtype A5. A cluster composed of four sheep virus sequences (SLO 31) was clearly divergent from all other subtypes in group A and could not be assigned to any of them. The virus sequences from one goat flock belonged solely to subtype B1, whereas virus sequences from more than one genotype were found to circulate within the other two goat flocks, belonging to subtype B1 (SLO 1 and SLO 37) and to genotype A (SLO 2 and 78–88 g). Two goat virus sequences (SLO 2) were found to belong to genotype A and could not be assigned to existing subtypes. One goat virus sequence (37–88 g) from flock 37 was clearly different from other sequences of this flock and was more closely related to genotype A sequences. We propose two new subtypes within genotype A, subtype A14 (SLO 2) and A15 (SLO 31).  相似文献   

Growth of the fan mussel Pinna nobilis (Linnaeus, 1758) (Mollusca: Bivalvia) in experimental cages in the South Adriatic Sea     

Valter Kožul  Nikša Glavić  Jakša Bolotin  Nenad Antolović 《Aquaculture Research》2012,44(1):31-40

Juveniles of the fan mussel Pinna nobilis were collected in Mali Ston Bay from October to December 2006. Cages with juveniles were placed at three depths: 1, 3 and 5 m. At the end of a 2‐year growth period at 1 m depths, the average length was 244.1 ± 22.9 mm, at 3 m depths, specimens averaged 244.0 ± 25.3 mm, and at 5 m depths, the average length was 231.1 ± 22.5 mm. The average monthly growth of shell length for the total experimental period was 8.7 ± 5.3, 8.4 ± 4.5 and 7.6 ± 4.4 mm, at 1, 3 and 5 m depths respectively.  相似文献   

Sorption Behaviour of Trichlorobenzenes and Polycyclic Aromatic Hydrocarbons in the Absence or Presence of Carbon Nanotubes in the Aquatic Environment     

Jelena Tričković  Marijana Kragulj Isakovski  Malcolm Watson  Snežana Maletić  Srđan Rončević  Božo Dalmacija  Zoltán Kónya  Ákos Kukovecz 《Water, air, and soil pollution》2016,227(10):374

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Race-specific response of Cucurbita germplasm to Pseudoperonospora cubensis     

Aleš Lebeda  Eva Křístková  Jana Roháčková  Božena Sedláková  Mark P. Widrlechner  Harry S. Paris 《Euphytica》2016,212(1):145-156

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Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici     

Aleš Lebeda  Barbora Mieslerová  Marek Petřivalský  Lenka Luhová  Martina Špundová  Michaela Sedlářová  Vladimíra Nožková-Hlaváčková  David A. C. Pink 《European journal of plant pathology / European Foundation for Plant Pathology》2014,138(3):569-596

Tomato powdery mildew (Oidium neolycopersici) is one of the most devastating diseases of cultivated tomatoes worldwide. Although the first epidemics were recorded more than 25 years ago many aspects of this host-pathogen interaction are still not well understood. Detailed morphological and molecular studies of the anamorphs confirmed that O. neolycopersici is phylogeneticaly close to Erysiphe aquilegiae var. ranunculi. Host range is rather broad, apart from Solanaceae hosts were found in the families Apocynaceae, Campanulaceae, Crassulaceae, Cistaceae, Cucurbitaceae, Linaceae, Malvaceae, Papaveraceae, Pedialiaceae, Scrophulariaceae, Valerianaceae a Violaceae. Non-host resistance within these families is not based on inhibition of formation of primary haustorium, however, on post-haustorial hypersensitive reponse and another type of non-hypersensitive resistance. Screening of wild Solanum species (previous Lycopersicon spp.) germplasm revealed valuable sources of resistance (S. habrochaites, S. pennellii, S. cheesmaniae, S. chilense, S. peruvianum). The main resistance mechanism was found to be a hypersensitive response (HR), in some cases followed by limited development of the pathogen. However, there is a broad variation in resistance response on the histological and cytological level. Interaction between many wild Solanum spp. and O. neolycopersici is race-specific, at least three races were differentiated. In some interspecific crosses (S. lycopersicum × S. habrochaites) adult plant resistance was observed. Biochemical studies focusing on production of reactive oxygen species (ROS) and peroxidase activity during infection of O. neolycopersici showed that production of ROS and activity of corresponding enzymes is related to activation of defence responses in genotypes of wild Solanum sect. Lycopersicon. The significance of nitric oxide (NO) in O. neolycopersici pathogenesis was supported by experiments with NO donors and scavengers. In moderately resistant genotype S. chmielewskii, treatment by heat stress caused slight deceleration of pathogen development, increased production of jasmonic acid (JA) and abscisic acid (ABA) and increased peroxidase activity in infected plants. The different degree of tomato resistance/susceptibility did not markedly change the rate and extent of photosynthetic response to O. neolycopersici; only minimal impairment of photosynthesis was found in both susceptible and moderately resistant genotypes during the first 9 days after inoculation. The accumulated evidence confirm a crucial role of localised increased production of ROS and reactive nitrogen species (RNS) in response to pathogen penetration into plant tissue and its involvement in the plant resistance responses including the initiation and progression of plant cell death in host wild Solanum species. Crucial points of further research are discussed.  相似文献   

Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production     

Nemec A  Jerin A  Zdovc I  Budefeld T  Verstraete FJ  Eržen D  Sentjurc M  Petelin M  Hitti T  Pavlica Z 《Research in veterinary science》2012,92(3):401-407

Twenty-four female BALB/c mice were orally inoculated with 10(8) CFU Escherichia coli ATCC 25922 and euthanized 2.5, 7, 13 and 25 h post-inoculation. The levels of organ nitric oxide (NO) and plasma endotoxin, TNF-alpha and nitrite/nitrate (NO(x)) were compared to those found in sham-inoculated mice, to evaluate systemic host-response to a low-level oral exposure to Gram-negative bacteria. Organ bacterial culture and immunohistochemistry for iNOS were performed on lungs, liver, kidneys and brain from all mice. Organ NO and plasma TNF-alpha levels were higher in E. coli-inoculated animals, but no differences were detected in plasma endotoxin levels, NO(x) or iNOS immunostaining for any of the animal groups. Single oral gavage with live E. coli stimulates an early systemic immune response in clinically healthy mice as evidenced by increased plasma TNF-alpha and organ NO levels, but bacteremia and endotoxemia are not related to this inflammatory response.  相似文献   

Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis     

Kovačević-Filipović M  Ilić V  Vujčić Z  Dojnov B  Stevanov-Pavlović M  Mijačević Z  Božić T 《Veterinary immunology and immunopathology》2012,145(1-2):120-128

Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.  相似文献   

Effect of (+)-limonene and 1-methoxy-2-propanol on Ips typographus response to pheromone blends     

Miroslav Blaženec  Rastislav Jakuš 《林业研究》2009,20(1):37-44

We compared two different strategies to increase the catches of Ips typographus (L.), particularly males, in pheromone-baited traps. The first of these strategies, the barrier approach, used alternating pheromone blends, targeting males and females respectively, in closely-spaced traps forming a barrier around forest stands. The second strategy, the single trap approach, used widely-spaced traps that were all baited with the same lure and intended to trap the highest possible numbers of males without compromising trapping of females. In the blend used for the barrier traps targeting primarily males, with a lower percentage of (4S)-cis-verbenol (cV), the (−)-α-pinene was replaced step wise with (+)-limonene at rates of 0%, 1%, 10%, 35%, 60% and 90%. This replacement had no significant effect on the numbers of responding I. typographus males, but there was a slight effect on the percentage of males caught. In the attractant blend for the barrier traps targeting females, with a higher percentage of cV, the 2-methyl-3-buten-2-ol (MB) was replaced with 1-methoxy-2-propanol (MP) in a similar fashion as for the male-specific blends. The replacement did not significantly affect the catch of females. Thus, it is possible to use the MP in the blend with cV and ipsdienol without significant change in catch efficacy. In the blends for single traps, the (−)-α- pinene was replaced with (+)-limonene and MB with MP. The replacement of (−)-α-pinene had only a slight effect on the percentage of males, but the results suggest that replacing MB with MP in the blend will not significantly reduce trapping efficacy. Foundation project: The research was supported by Slovak Research and Development Agency (APVV-51-P06005 and APVV-27-P05205) and by the Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences (2/6153/26)  相似文献