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Jun-Gyu Park Hyun-Jeong Kim Jelle Matthijnssens Mia Madel Alfajaro Deok-Song Kim Kyu-Yeol Son Hyoung-Jun Kwon Myra Hosmillo Eun-Hye Ryu Ji-Yun Kim Rohani B Cena Ju-Hwan Lee Mun-Il Kang Sang-Ik Park Kyoung-Oh Cho 《Veterinary research》2013,44(1):88
Direct interspecies transmissions of group A rotaviruses (RVA) have been reported under natural conditions. However, the pathogenicity of RVA has never been directly compared in homologous and heterologous hosts. The bovine RVA/Cow-tc/KOR/K5/2004/G5P[7] strain, which was shown to possess a typical porcine-like genotype constellation similar to that of the G5P[7] prototype RVA/Pig-tc/USA/OSU/1977/G5P9[7] strain, was examined for its pathogenicity and compared with the porcine G5P[7] RVA/Pig-tc/KOR/K71/2006/G5P[7] strain possessing the same genotype constellation. The bovine K5 strain induced diarrhea and histopathological changes in the small intestine of piglets and calves, whereas the porcine K71 strain caused diarrhea and histopathological changes in the small intestine of piglets, but not in calves. Furthermore, the bovine K5 strain showed extra-intestinal tropisms in both piglets and calves, whereas the porcine K71 strain had extra-intestinal tropisms in piglets, but not in calves. Therefore, we performed comparative genomic analysis of the K71 and K5 RVA strains to determine whether specific mutations could be associated with these distinct clinical and pathological phenotypes. Full-length sequencing analyses for the 11 genomic segments for K71 and K5 revealed that these strains were genetically nearly identical to each other. Two nucleotide mutations were found in the 5′ untranslated region (UTR) of NSP5 and the 3′ UTR of NSP3, and eight amino acid mutations in VP1-VP4 and NSP2. Some of these mutations may be critical molecular determinants for RVA virulence and/or pathogenicity. 相似文献
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Petra Mayerhofer Joseph Alcamo Maximilian Posch Jelle G. Van Minnen 《Water, air, and soil pollution》2001,130(1-4):1151-1156
The aim of the AIR-CLIM project is to perform an integrated analysis of the linkages between climate change and regional air pollution in Europe and to produce results that are relevant to European policy-making. Key elements of the analysis are on the impact side the exceedances of critical thresholds for air pollution and global change and on the cost side the estimates of costs to reduce emissions of greenhouse gases and air pollutants. The integrated modeling framework set up to meet these objectives consists of two state-of-the-art integrated models covering regional air pollution in Europe (RAINS) and global climate change (IMAGE), supplemented by new components. Based on a preliminary analysis it can be stated that climate change will make European vegetation in most regions less sensitive to acid deposition. Taking into account the emission trends the impacts of regional air pollution will decrease while the impacts of climate change increase. Different problems will be important in different regions: regional air pollution in Central and northern Europe, and climate change in southern Europe. 相似文献
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Alejandra Badaracco Jelle Matthijnssens Sandra Romero Elisabeth Heylen Mark Zeller Lorena Garaicoechea Marc Van Ranst Viviana Parreño 《Veterinary microbiology》2013,161(3-4):247-254
The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation. 相似文献
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Keukeleire JD Janssens I Heyerick A Ghekiere G Cambie J Roldan-Ruiz I Bockstaele EV Keukeleire DD 《Journal of agricultural and food chemistry》2007,55(1):61-66
The concentrations of alpha-acids, beta-acids, desmethylxanthohumol, and xanthohumol were monitored in the hop varieties Admiral (A), Wye Challenger (WC), and First Gold (FG) during the harvest seasons of 2003 through 2005. Hops grown under an organic regimen were compared to plants grown conventionally in hop fields in close vicinity. The concentrations of the key compounds depended very much on climatological conditions showing, in general, highest levels in poorest weather conditions (2004). Of the three varieties studied, FG was the only one showing a clear trend for higher concentrations of secondary metabolites under organic growing conditions than under conventional farming conditions. Cultivation of A and WC seems to be very sensitive to climatic conditions and environmental stresses caused by pests and diseases, thereby leading to various results. WC proved to be a rich source of bioactive chalcones, particularly desmethylxanthohumol. 相似文献
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Mojtaba Keykhasaber Bart P. H. J. Thomma Jelle A. Hiemstra 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(2):323-339
Verticillium dahliae colonizes the xylem vessels of susceptible host plants. Hence it can be expected that the distribution of the fungus as well as disease progress will be influenced by the anatomy of the xylem of that host. Here, we studied the spatial and temporal distribution of V. dahliae in relation to recovery from disease symptoms in young European ash (Fraxinus excelsior) and Norway maple (Acer platanoides) trees that differ in vascular anatomy. Quantifying the amount of V. dahliae DNA at different heights in the stem of inoculated trees at different time points after inoculation showed that, in the year of inoculation, the speed of colonization of these two species by V. dahliae was similar. Nevertheless, in the year after inoculation disease incidence and also quantities of V. dahliae detected in maple trees were significantly higher than in ash trees, suggesting that the xylem of ash trees is much less supportive for growth and survival of V. dahliae than that of maple trees. Moreover, in this second year V. dahliae could not be re-isolated from the wood of ash trees that had recovered from disease and was only rarely detected by PCR, only in xylem of the previous year and never in the current xylem. In contrast, V. dahliae easily was detected in the wood of diseased ash and maple trees. Furthermore, despite the presence of a layer of parenchyma cells between growth rings in ash trees, in symptomatic ash trees V. dahliae was present in the xylem of the new growth ring. We also observed that V. dahliae can move downward from the point of inoculation into the root collar, which possibly provides a way for infection of new growth rings by circumventing the physical barriers within the stem xylem. 相似文献
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Mes JJ Weststeijn EA Herlaar F Lambalk JJ Wijbrandi J Haring MA Cornelissen BJ 《Phytopathology》1999,89(2):156-160
ABSTRACT A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main groups that coincide with vegetative compatibility groups (VCGs). In addition, several single-member VCGs were identified that could not be grouped in one of the two main RAPD clusters. This suggests that F. oxysporum f. sp. lycopersici is a polyphyletic taxon. To assign avirulence genotypes to race 1 isolates, they were tested for their virulence on a small set of tomato lines (Lycopersicon esculentum), including line OT364. This line was selected because it shows resistance to race 2 isolates but, unlike most other race 2-resistant lines, susceptibility to race 1 isolates. To exclude the influence of other components than those related to the race-specific resistance response, we tested the virulence of race 1 isolates on a susceptible tomato that has become race 2 resistant by introduction of an I-2 transgene. The results show that both line OT364 and the transgenic line were significantly affected by four race 1 isolates, but not by seven other race 1 isolates nor by any race 2 isolates. This allowed a subdivision of race 1 isolates based on the presence or absence of an avirulence gene corresponding to the I-2 resistance gene. The data presented here support a gene-for-gene relationship for the interaction between F. oxysporum f. sp. lycopersici and its host tomato. 相似文献
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V. Martella Krisztián Bányai Jelle Matthijnssens Canio Buonavoglia Max Ciarlet 《Veterinary microbiology》2010,140(3-4):246-255
Rotaviruses are important enteric pathogens of humans and animals. Group A rotaviruses (GARVs) account for up to 1 million children deaths each year, chiefly in developing countries and human vaccines are now available in many countries. Rotavirus-associated enteritis is a major problem in livestock animals, notably in young calves and piglets. Early in the epidemiological GARV studies in humans, either sporadic cases or epidemics by atypical, animal-like GARV strains were described. Complete genome sequencing of human and animal GARV strains has revealed a striking genetic heterogeneity in the 11 double stranded RNA segments across different rotavirus strains and has provided evidence for frequent intersections between the evolution of human and animal rotaviruses, as a result of multiple, repeated events of interspecies transmission and subsequent adaptation. 相似文献
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Dynamics of the nitrous oxide reducing community during adaptation to Zn stress in soil 总被引：1，自引：0，他引：1
Stefan Ruyters Jelle Mertens Isabelle T’Seyen Dirk Springael Erik Smolders 《Soil biology & biochemistry》2010,42(9):1581-1587
Laboratory studies show that the nitrous oxide (N2O) reduction rate in soil is strongly inhibited by trace metal contamination; however, this effect appears transient. Here we assess if this recovery is due to microbial adaptation associated with shifts in community composition. Soils were spiked with zinc chloride (0-5000 mg Zn kg−1) in a factorial design with 3 application rates of organic matter (OM), i.e. 0, 2 and 4 g milled hay kg−1, to accelerate growth and, potentially, adaptation rate. The soil treatments were incubated outdoors with free drainage during 1 year and periodically sampled. The potential N2O reduction rate, measured in an anaerobic laboratory assay, was inhibited by Zn during the first 2 months after spiking with 50% inhibition at 500-1000 mg Zn kg−1. After 6 months exposure, the N2O reduction rate recovered to at least 80% of the rate in the control treatment in the series receiving OM up to the largest Zn dose, but strong inhibition remained in the series which did not receive OM. In this series recovery was only observed after 12 months exposure. Soil pore water Zn concentrations did not explain the recovery of the N2O reduction rate in the control series suggesting that recovery is due to adaptation and not to reduced Zn bioavailability. The faster recovery in the series receiving OM was partially, but not fully related to the effects of OM on Zn bioavailability. The recovery at all Zn and OM treatments co-varied with a recovery of nosZ gene abundance from about 1 × 107 copies g−1 soil in the soil treatments with decreased activity to 5 × 108 copies g−1 soil in the other soil treatments. The nosZ gene DGGE profile of the soil microbial communities revealed minor changes in the nosZ containing community. This study strongly suggests that the transient effects of trace metal inhibition of N2O reduction is due to the development of a Zn tolerant denitrifying community. 相似文献
20.
Resistance and resilience of zinc tolerant nitrifying communities is unaffected in long-term zinc contaminated soils 总被引：1，自引：0，他引：1
Jelle Mertens Stefan Ruyters Dirk Springael Erik Smolders 《Soil biology & biochemistry》2007,39(7):1828-1831
Zinc (Zn) tolerant nitrifying communities were previously identified in soil samples of a long-term Zn contaminated transect towards a galvanised pylon. We questioned whether Zn tolerance increased the vulnerability of the nitrifying communities to stressors. The influence of pesticide addition, freeze-thaw or dry-wet cycles on the soil nitrification (‘functional stability’) was assessed in a series of these soils representing a Zn contamination and Zn tolerance gradient. The immediate effect of the stressors to the nitrification (‘resistance’) and the residual effect after 3 weeks incubation (‘resilience’) were determined. Our results show that neither resistance nor resilience to these stressors was affected by adaptation of the nitrifying communities to elevated Zn concentrations in the long-term contaminated soils. 相似文献