Evaluation of Anti-Nociceptive and Anti-Inflammatory Activities of a Heterofucan from Dictyota menstrualis     

Ivan Rui Lopes Albuquerque  Sara Lima Cordeiro  Dayanne Lopes Gomes  Juliana Luporini Dreyfuss  Luciana Guimar?es Alves Filgueira  Edda Lisboa Leite  Helena Bonciani Nader  Hugo Alexandre Oliveira Rocha 《Marine drugs》2013,11(8):2722-2740

Fucan is a term that defines a family of homo- and hetero-polysaccharides containing sulfated l-fucose in its structure. In this work, a heterofucan (F2.0v) from the seaweed, Dictyota menstrualis, was evaluated as an antinociceptive and anti-inflammatory agent. F2.0v (20.0 mg/kg) inhibits 100% of leukocyte migration into the peritoneal cavity after chemical stimulation. However, F2.0v does not alter the expression of interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), as well as tumor necrosis factor alpha (TNF-α). F2.0v (20.0 mg/kg) has peripheral antinociceptive activity with potency similar to dipyrone. On the other hand, it had no effect on pain response on the hot plate test. Confocal microscopy analysis and flow cytometry showed that F2.0v binds to the surface of leucocytes, which leads us to suggest that the mechanism of action of anti-inflammatory and antinociceptive F2.0v is related to its ability to inhibit the migration of leukocytes to the site of tissue injury. In summary, the data show that F2.0v compound has great potential as an antinociceptive and anti-inflammatory, and future studies will be performed to further characterize the mechanism of action of F2.0v.  相似文献   

Elevated [CO2] and warming increase the macronutrient use efficiency and biomass of Stylosanthes capitata Vogel under field conditions     

Juliana Mariano Carvalho  Rafael Ferreira Barreto  Renato de Mello Prado  Eduardo Habermann  Carlos Alberto Martinez  Roberto Botelho Ferraz Branco 《Journal of Agronomy and Crop Science》2020,206(5):597-606

Stylosanthes capitata Vogel is a C3 forage legume widely cultivated in tropical and subtropical pastures. However, the nutrient dynamics of this species under future climate change is unknown. Therefore, this study aimed to evaluate the nutrient content, nutrient accumulation, nutrient use efficiency and growth of S. capitata exposed to increased [CO₂] and temperature under field conditions using two levels of atmospheric [CO₂] (ambient and elevated—600 ppm) and two canopy temperature (ambient and elevated—2°C). Treatments were applied at field conditions, for 30 days, using a free-air carbon dioxide enrichment (FACE) and a free-air temperature-controlled enhancement (T-FACE) systems. Warming showed no effects on macronutrient content, but increased the accumulation of nitrogen, potassium, calcium, magnesium and sulphur, the nutrient use efficiency and root dry mass. Elevated [CO₂] alone had no effect on most of the parameters evaluated. However, the combination of elevated [CO₂] with warming improved the nutrient accumulation, nutrient use efficiency and whole-plant growth more than under isolated conditions of elevated [CO₂] or warming. Based on our short-term results, we concluded that an increment of atmospheric [CO₂] and temperature will benefit S. capitata growth, suggesting no alterations in the actual fertilizer programs for this species.  相似文献   

The effect of lufenuron, a chitin synthesis inhibitor, on oogenesis of Rhodnius prolixus     

Juliana F. Mansur  Amanda S. Santos  Isabela B. Ramos  Ednildo A. Machado  Carlos R. Kaiser  Hatisaburo Masuda  Ana Maria H. Vasconcellos  Mônica F. Moreira 《Pesticide biochemistry and physiology》2010,98(1):59-67

The effects of lufenuron, a chitin synthesis inhibitor, on oogenesis of Rhodnius prolixus were evaluated. Lufenuron-treated females lay eggs that are pink, but change color with time, acquiring a gray color. The eggs are abnormal in shape and become dehydrated. The percentage of eggs that hatch is decreased compared with the eggs from non-treated females. Digestion of ovaries with KOH yielded an insoluble white material, which was identified as chitin based on infrared and proton NMR spectral analyses along with standard commercial chitin. Glucosamine was detected by HPLC as the major product of acid hydrolysis of the KOH-insoluble material prepared from the ovary. Furthermore, presence of chitin in ovary was confirmed through fluorescent microscopy technique using a wheat germ agglutinin probe and by re-treatment with Streptomyces griseus chitinase that resulted in reduction of chitin-derived fluorescence. Micrographs of ovaries, from animals treated with lufenuron, showed some space between the follicular epithelium and the oocyte membrane. The in vivo incorporation of radioactive N-acetylglucosamine into chitin was inhibited by the presence of lufenuron. These data suggest that chitin present in ovaries of R. prolixus is an important component of the eggs and is involved in oogenesis. Exposure to lufenuron reduces the size of oocytes, number of chorionated oocytes, and also the incorporation of N-acetylglucosamine into chitin in the ovaries. In addition, lufenuron-induced oosorption, decreased the number of eggs laid, modified egg shape and color and reduced the viability of the eggs. Therefore, chitin or chitin-like component of ovaries may be considered as a new target for controlling R. prolixus populations.  相似文献   

Thermal and Chemical Properties of Gelatin from Tilapia (Oreochromis niloticus) Scale     

Maria Emanuella O. Martins  Juliana R. Sousa  Rayanne L. Claudino  Sarah Cristina O. Lino  Diego Alves do Vale  André Luis C. Silva 《Journal Of Aquatic Food Product Technology》2013,22(10):1120-1133

ABSTRACT

Gelatin from the scale of Nile tilapia was extracted and characterized chemically, thermally, and structurally. Scales of Nile tilapia were subjected to acid pretreatment and extraction with water at 60°C. The process yielded 12.1% (m/m) on a dry basis. The 6.67% gelatin solution had a gel strength of 233.5 ± 14g. The amino acid composition revealed that 20.02% was comprised of imino acids and glycine (34.49%) as the most abundant amino acids. The scale gelatin had α1, α2, and β subunits and was thermally stable up to 200ºC. The Fourier transform infrared spectrum showed absorption in the regions of amides A, I, II, and III. The characterization of tilapia scale gelatin showed that the obtained product was similar to commercial gelatin, thereby demonstrating its potential application.  相似文献   

Responses to ROS inducer agents in zebrafish cell line: differences between copper and UV-B radiation     

Regina Coimbra Rola  Luis Fernando Marins  Luiz Eduardo Maia Nery  Carlos Eduardo da Rosa  Juliana Zomer Sandrini 《Fish physiology and biochemistry》2014,40(6):1817-1825

Fish are commonly exposed to environmental pollutants, which in turns could induce an oxidative stress. So, it is important to understand the effects and the responses elicited by these toxicants in fish species, being fish cell lines important tools for this purpose. Thus, the aim of the present study was to compare the effects of copper and UV-B radiation exposure on zebrafish hepatocytes (ZFL lineage) in terms of reactive oxygen species (ROS) levels, sulfhydril groups content and mRNA levels of important genes related to cellular response to toxic agents. Exposure of ZFL cells to UV-B radiation (23.3 mJ/cm²) significantly increased levels of intracellular ROS and mRNA of both superoxide dismutase isoforms (sod1 and sod2), three glutathione S-transferase isoforms (gstα, gstµ and gstπ) and a heat shock protein (hsp70). However, no changes in nonprotein sulfhydryl groups (NP-SH) content, as well as in the mRNA levels of genes related to glutathione (GSH) synthesis and recycling, were observed. Contrary to this, copper exposure (20 mg/L) diminished NP-SH content and increased the levels of mRNA of genes related to GSH synthesis (gclc and gs). Moreover, copper exposure increases the mRNA levels of some genes related to antioxidant defenses (gpx and gstπ), biotransformation reactions (cyp1a1) and protein repair (hsp70). In conclusion, these results demonstrated that both toxicants could increase ROS levels in ZFL cell line, but the responses are different, which could be related to activation of different signaling pathways.  相似文献   

An immunological method for granulovirus detection in larvae of Tuta absoluta: searching for isolates with prospects for biological control of this pest in Colombia     

Juliana Gómez-Valderrama  Lorena Herrera  Daniel Uribe-Vélez  Miguel López-Ferber  Laura Villamizar 《国际虫害防治杂志》2014,60(2):136-143

The tomato leafminer Tuta absoluta is an important insect pest of tomato crops worldwide. Biological control using entomopathogenic viruses such as isolates of Phthorimaea operculella granulovirus (PhopGV) can be a promising tool for suitably controlling this pest. However to date, few viral isolates from T. absoluta have been reported. Therefore, the aim of this study was to develop an economic, fast and accurate immunological technique for granulovirus detection and to evaluate viral presence in T. absoluta larvae from field samples collected in Colombia. Polyclonal antibodies against PhopGV were produced by using hens and rabbits. The production system in hen was selected as the most efficient and simple method. Then, a dot-blot test was developed, demonstrating the ability of antibodies to recognize granulovirus. Finally, granulovirus presence in 1186 T. absoluta larvae collected in the most important tomato production areas in Colombia was evaluated, and eight isolates were found in Boyacá and Cundinamarca (0.7% of occurrence). These results constitute the first report of a granulovirus isolated from T. absoluta larvae which had been naturally infected in Colombia's tomato producing-fields, and thereby validates the use of the immunological technique to detect granulovirus in field conditions.  相似文献   

A ten years (2007–2016) retrospective serological survey for <Emphasis Type="Italic">Seneca Valley virus</Emphasis> infection in major pig producing states of Brazil     

Viviane Saporiti  Juliana T. T. Fritzen  Cesar Feronato  Raquel A. Leme  Zelia I. P. Lobato  Alice F. Alfieri  Amauri A. Alfieri 《Veterinary research communications》2017,41(4):317-321

Seneca Valley virus (SVV) is the etiological agent of vesicular disease in pigs, clinically indistinguishable of classical viral vesicular infections, including foot-and-mouth disease. The first outbreaks of SVV infection in Brazil were reported in 2014. However, it was not known whether the virus was circulating in Brazilian pig herds before this year. This study is a retrospective serological investigation of porcine health status to SVV in Brazil. Serum samples (n = 594) were grouped in before (2007–2013, n = 347) and after (2014–2016, n = 247) SVV outbreaks in Brazil. Twenty-three pig herds were analyzed, of which 19 and 4 were sampled before and after the beginning of SVV outbreaks, respectively. Two herds sampled after 2014 presented animals with SVV-associated clinical manifestations, while the other two housed asymptomatic pigs. Anti-SVV antibodies were evaluated by virus neutralization test. The results demonstrated that pig herds of different Brazilian geographical regions and distinct pig categories were negative to anti-SVV antibodies in sera obtained before 2014. Antibodies to SVV were detected only in serum samples obtained after 2014, particularly in herds with the presence of pigs with SVV-clinical signs. These results present robust serological evidence that the SVV was not present in the major Brazilian pig producing regions prior to 2014.  相似文献   

Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm     

Gerardo M. Giomi  Enrique D. Kreff  Juliana Iglesias  Carolina M. Fauguel  Mariana Fernandez  Maria Silvina Oviedo  Daniel A. Presello 《Euphytica》2016,211(3):287-294

  相似文献   

In vitro and in vivo susceptibility of two-drug and three-drug combinations of terbinafine, itraconazole, caspofungin, ibuprofen and fluvastatin against Pythium insidiosum     

Argenta JS  Alves SH  Silveira F  Maboni G  Zanette RA  Cavalheiro AS  Pereira PL  Pereira DI  Sallis ES  Pötter L  Santurio JM  Ferreiro L 《Veterinary microbiology》2012,157(1-2):137-142

The present study investigated the in vitro inhibitory activity of terbinafine, itraconazole, caspofungin, fluvastatin and ibuprofen against 15 isolates of Pythium insidiosum in double and triple combinations and determined in vivo correlations using rabbits with experimental pythiosis. The minimal inhibitory concentration (MIC) was determined in accordance with the Clinical and Laboratory Standards Institute M 38-A2 protocol (2008), and the in vitro interactions were evaluated using a checkerboard microdilution method. For the in vivo study, 20 rabbits inoculated with P. insidiosum zoospores were divided into four groups: group 1 was treated with terbinafine and itraconazole; group 2 was treated with terbinafine, itraconazole and fluvastatin; group 3 was treated with terbinafine and caspofungin; and group 4 was the control group. Combinations of terbinafine with caspofungin or ibuprofen were synergistic for 47% of the isolates, and antagonism was not observed in any of the double combinations. The triple combinations were mostly indifferent, but synergism and antagonism were also observed. In the in vivo study, the histological aspect of the lesions was similar among the groups, but group 2 showed the lowest amount of hyphae and differed significantly from the other groups.  相似文献   

Evaluation of the Integrity of Horse Hoof Dermal and Epidermal Tissues Collected by Dorsal Transmural Access     

Kamila Gravena  Rita de Cássia de Lima Sampaio  Deborah Penteado Martins Dias  Paulo Aléscio Canola  Juliana Regina Peiró  José Corrêa de Lacerda-Neto 《Journal of Equine Veterinary Science》2012

The purpose of this study was to evaluate the integrity of dermal and epidermal tissues collected by dorsal transmural access. Eight healthy adult horses were subjected to two biopsies of the right hoof, with a 24-hour interval. To ensure hoof integrity, a radiographic evaluation with dorsopalmar and lateromedial positions was performed. The animals were sedated, and the pedal tissue was collected from two sites that were 2 cm distal to the coronary band. For each biopsy, the hoof wall was perforated using an aluminum oxide drill bit, 4.8 mm in diameter that was connected to a drilling machine, yielding a hole of approximately 9.0 mm. The perforation procedure was performed slowly in the stratum medium until 1 mm of it remained, which was determined by evaluating the movement in response to pressure applied with a Halstead forceps. Laminar deep incisions were performed with a number 11 scalpel blade until the distal phalanx was reached. The laminar tissue was then removed using a Frahm scaler. A cuboid laminar sample measuring approximately 5 mm at the base and 7 mm in height was obtained. The samples were suitable for analysis by histology and transmission electron microscopy showing that the ends of the secondary epidermal laminae were round, the basement membrane was intact between the secondary epidermal laminae and secondary dermal laminae, and the ultrastructural analysis of the cells showed their structural integrity.  相似文献