Genetic structuring among populations of the great egret,Ardea alba egretta,in major Brazilian wetlands          下载免费PDF全文

Thaís Camilo Corrêa  Silvia Nassif Del Lama  Juliana Rossi De Souza  Carolina Isabel Miño 《水产资源保护:海洋与淡水生态系统》2016,26(2):333-349

Ovicidal activity of seven <Emphasis Type="Italic">Pochonia chlamydosporia</Emphasis> fungal isolates on <Emphasis Type="Italic">Ascaris suum</Emphasis> eggs     

Ferreira SR  Araújo JV  Braga FR  Araujo JM  Carvalho RO  Silva AR  Frassy LN  Freitas LG 《Tropical animal health and production》2011,43(3):639-642

The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.  相似文献   

Identification of Hammondia heydorni oocysts by a heminested-PCR (hnPCR-AP10) based on the H. heydorni RAPD fragment AP10     

Soares RM  Lopes EG  Keid LB  Sercundes MK  Martins J  Richtzenhain LJ 《Veterinary parasitology》2011,175(1-2):168-172

Toxoplasma gondii, Hammondia hammondi, Neospora caninum, Neospora hughesi and Hammondia heydorni are members of the Toxoplasmatinae sub-family. They are closely related coccidians with similarly sized oocysts. Molecular diagnostic techniques, especially those based on polymerase chain reaction (PCR), can be successfully applied for the differentiation of Hammondia-like oocysts. In this paper, we describe a rapid and simple method for the identification of H. heydorni oocysts among other members of the Toxoplasmatinae sub-family, using a heminested-PCR (hnPCR-AP10) based on a H. heydorni RAPD fragment available in molecular database. DNA of oocysts of H. heydorni yielded a specific fragment of 289-290 bp in the heminested-PCR assay. No product was yielded when the primers were used for the amplification of DNA extracted from T. gondii, N. caninum, N. hughesi and H. hammondi, thus allowing the differentiation of H. heydorni among other members of the Toxoplasmatinae sub-family. The hnPCR-AP10 was capable of detecting H. heydorni genetic sequences from suspensions with at least 10 oocysts. In conclusion, the hnPCR-AP10 proved to be a reliable method to be used in the identification of H. heydorni oocysts from feces of dogs.  相似文献   

Leishmania chagasi infection in cats with dermatologic lesions from an endemic area of visceral leishmaniosis in Brazil     

Vides JP  Schwardt TF  Sobrinho LS  Marinho M  Laurenti MD  Biondo AW  Leutenegger C  Marcondes M 《Veterinary parasitology》2011,178(1-2):22-28

Although dogs are considered the main domestic reservoirs for Visceral Leishmaniosis (VL), which is caused in the Americas by Leishmania chagasi, infected cats have also been recently found in endemic areas of several countries and became a public health concern. Accordingly, the purpose of this study was to evaluate cats with dermatologic lesions from an endemic area of VL and the natural infection of L. chagasi. A total of 55 cats were selected between April 2008 and November 2009 from two major animal shelters of Ara?atuba, Southeastern Brazil. All cats underwent general and dermatologic examinations, followed by direct parasitological examination of lymphoid organs, immunosorbent assay (ELISA) and indirect immunofluorescence (IFAT). In addition, detection of amastigotes was performed by immunohistochemistry (IHC) in skin lesions of all cats. VL was diagnosed in 27/55 (49.1%) cats with dermatological problems. Amastigotes were found in lymphoid organs of 10/27 (37.0%) cats; serology of 14/27 (51.9%), 6/27 (22.2%) and 5/27 (18.5%) cats was positive for ELISA, IFAT and both, respectively. The IHC identified 9/27 (33.3%) cats; 5/27 (18.5%) were positive only for IHC and therefore increased the overall sensitivity. Specific FIV antibodies were found in 6/55 (10.9%) cats, of which 5/6 (83.3%) had leishmaniosis. Real time PCR followed by amplicon sequencing successfully confirmed L. chagasi infection. In conclusion, dermatological lesions in cats from endemic areas was highly associated to visceral leishmaniosis, and therefore skin IHC and differential diagnosis of LV should be always conducted in dermatological patients in such areas.  相似文献   

Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains     

Mar��a Soledad Fuentes  Juliana Mar��a S��ez  Claudia Susana Benimeli  Mar��a Julia Amoroso 《Water, air, and soil pollution》2011,222(1-4):217-231

The current study aimed to compare lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of lindane. Furthermore, the role of bacterial consortia of lindane-degrading strains was examined in lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L^-1 lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual lindane concentration and SDA and could be a promising tool for lindane biodegradation.  相似文献   

Grating Visual Acuity in phakic,aphakic, and pseudophakic Poodles     

Anglica M. V. Safatle  Tatiana A. de Moraes  Ana R. Eyherabide  Arthur G. Fernandes  Juliana S. Jorge  Laysa M. C. R. Carvalho  Emily A. K. Rodriguez  Denise Otsuki  Aline A. Bolzan  Paula Y. Sacai 《Veterinary ophthalmology》2020,23(5):879-883

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Ruminal and histological characteristics and nitrogen balance in lamb fed diets containing cactus as the only roughage     

da Silva  Kleitiane Balduino  de Oliveira  Juliana Silva  Santos  Edson Mauro  Cartaxo  Felipe Queiroga  Guerra  Ricardo Romão  do Nascimento de Souza  Aelson Fernandes  Muniz  Ana Cecília Souza  de Lima Cruz  Gabriel Ferreira 《Tropical animal health and production》2020,52(2):637-645

Tropical Animal Health and Production - This study examined rumen fermentation, histological, blood, and urinary characteristics as well as ammoniacal nitrogen concentration, pH, nitrogen balance,...  相似文献   

Evaluation of reproductive parameters in male Neotropical bats during dry and rainy months in a specific area of the Cerrado biome     

Juliana Lis Mendes de Brito  Thales Simioni Amaral  Ludmilla Moura de Souza Aguiar  Carolina Madeira Lucci 《Anatomia, histologia, embryologia》2020,49(2):307-314

The aim of this study was to analyse the reproductive aspects of male bats of three common species of the Phyllostomidae family: Artibeus lituratus, Platyrrhinus lineatus and Sturnira lilium, during dry and rainy months in a specific area of the Cerrado biome. Body weight was significantly higher during the dry months for S. lilium. The gonadosomatic index (GSI) and testicular weight were not significantly different between dry and rainy periods. The tubular parameters were significantly bigger in A. lituratus than in the other two species during both periods. No difference in the tubular/interstitial ratio was observed in any of the species during both periods. In both periods, all sperm cells and germ cell developmental stages were visible on seminiferous tubules whereas sperm cells were observed in epididymides of all sampled animals. The percentage of morphologically normal sperm was low (35%–60%), with no difference between periods. Spermatozoa from A. lituratus presented a leaf-shaped head, while the head was round-shaped in the other two species. In conclusion, our data suggest that males from the three studied species did not present reproductive latency during the most critical weather periods (dry and rainy months) in the metropolitan region of Brasilia, Brazil.  相似文献   

Growth attributes,xylem sap composition,and photosynthesis in common bean as affected by nitrogen and phosphorus deficiency     

Juliana D. Lima  Fábio M. Da Matta  Paulo R. Mosquim 《Journal of plant nutrition》2013,36(7):937-947

The effects of nitrogen (N‐) and phosphorus (P‐) deficiency, isolatedly or in combination, on growth, nitrogenous fraction, and inorganic phosphate in xylem exudade, and photosynthesis of common bean (Phaseolus vulgaris L. cv. Negrito) were investigated. Plants were grown in nutrient solution adjusted daily to pH 5.5 and aerated continuously. Ten days after emergence mineral deficiency was imposed. Plants were then supplied with high N (7.5 mol m^‐3) or low N (0.5 mol m^‐3), and also with high P (0.5 mol m^‐3) or low P (0.005 mol m^‐3). All sampling and measurements were made 28 days after emergence. N‐ or P‐deprivation brought about large decreases in total leaf area by inhibiting the emergence of new leaves and primarily the expansion of the leaves. The specific leaf area did not change under N‐ but decreased under P‐limitation. The decreased shoot to root ratio in all deficiency treatments was a consequence of a lowering mass of above‐ground organs, especially of leaves.

The content of chlorophylls declined significantly only under N‐deficiency alone; carotenoids declined under both N‐ and combined N‐ and P‐limitation. No alteration in amino acid concentration in xylem exudate occurred in plants experiencing N‐starvation, while ureides increased by 79%, and nitrate and inorganic phosphate decreased greatly. Under P‐deprivation, amino acids and nitrate in xylem sap dropped by about half; ureides were held relatively constant, and phosphate was severely depressed. Total upward translocation of N through xylem was estimated to be about 16% higher in N‐deficient plants than in plants without mineral limitation, but leaf N levels in the former were lower as compared to control plants. The net carbon (C) assimilation decreased similarly regardless of the imposed deficiency treatment. Such a decrease was mainly determined by non‐stomatal factors. In general, no additive effect between N‐ and P‐limitation on any of measured parameters was observed.  相似文献   

Dynamics of Agricultural Information and Effect on Income of Melon Farmers in Enugu-Ezike Agricultural Zone of Enugu State,Nigeria     

Juliana Chinasa Iwuchukwu  George Okechukwu Ekeh  Michael C. Madukwe 《Journal Of Agricultural & Food Information》2013,14(1):62-70

ABSTRACT

The study assessed effects of agricultural information on the income of melon farmers. Ninety melon farmers constituted the sample for the study. Data were collected through an interview average annual income of the respondents from melon only was ?7,455.4, while their total average schedule, while percentage mean score and correlation were used in the analysis. Findings reveal that the monthly income was ?1,223. About 62 and 42% of the respondents, respectively, did not receive information on melon production and general agriculture. Thus, a positive and significant relationship exists between the number of times respondents received information on melon/general agriculture and their incomes.  相似文献