Persulfate Interaction with Tropical Soils     

Fernanda C. Oliveira  Juliana G. Freitas  Sheila A. C. Furquim  Renata M. Rollo  Neil R. Thomson  Luís R. F. Alleoni  Claudio A. O. Nascimento 《Water, air, and soil pollution》2016,227(9):343

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Boron and zinc inhibit embryonic development,hatching and reproduction of Meloidogyne incognita     

Evelyn Aline Arendt Couto  Juliana Kath  Juliana Aparecida Homiak  Heriksen Higashi Puerari 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(4):346-352

Nematodes have difficult control and complex handling, but considering the physiological and biochemical changes that micronutrients promoting in plants, there is possibility that the supply with these chemicals increases the resistance of plants against nematodes. Thus, the present study aimed to assess the effect of the application of boron and zinc on the reproduction of Meloidogyne incognita, embryonic development and juvenile nematode hatching. Nematode reproduction was evaluated in tomato plant inoculated with 2000 eggs and treated in the aerial part with boron or zinc at the following doses: 0, 1/2, 1×, 2× and 4× the manufacturer's recommendation (100 and 30?g/L, respectively), with the plants assessed 60 days after inoculation. For assessment of embryonic development and juvenile hatching, 1?mL nematode suspension was placed in Petri dishes containing 9?mL of the same doses of boron and zinc, and assessment occurred four and eight days after incubation. Results obtained showed that boron controlled nematode population at the dose of 400?g/L and promoted juvenile hatching when used at maximum dosage on the eighth day. Zinc reduced the number of galls and the number of eggs at the dose of 60?g/L, but did not exhibit direct effect on nematode.  相似文献   

Characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish     

Caio Rodrigo Dias de Assis  Amanda Guedes Linhares  Vagne Melo Oliveira  Renata Cristina Penha França  Juliana Ferreira Santos  Marina Marcuschi  Elba Verônica Matoso Maciel Carvalho  Ranilson Souza Bezerra  Luiz Bezerra Carvalho Jr. 《Fish physiology and biochemistry》2014,40(6):1659-1668

Brain cholinesterases from four fish (Arapaima gigas, Colossoma macropomum, Rachycentron canadum and Oreochromis niloticus) were characterized using specific substrates and selective inhibitors. Parameters of catalytic efficiency such as activation energy (AE), k _cat and k _cat/k _m as well as rate enhancements produced by these enzymes were estimated by a method using crude extracts described here. Despite the BChE-like activity, specific substrate kinetic analysis pointed to the existence of only acetylcholinesterase (AChE) in brain of the species studied. Selective inhibition suggests that C. macropomum brain AChE presents atypical activity regarding its behavior in the presence of selective inhibitors. AE data showed that the enzymes increased the rate of reactions up to 10¹² in relation to the uncatalyzed reactions. Zymograms showed the presence of AChE isoforms with molecular weights ranging from 202 to 299 kDa. Values of k _cat and k _cat/k _m were similar to those found in the literature.  相似文献   

Apple Phenolic Extracts Strongly Inhibit α-Glucosidase Activity     

de Oliveira Raphaelli  Chirle  dos Santos Pereira  Elisa  Camargo  Taiane Mota  Vinholes  Juliana  Rombaldi  Cesar Valmor  Vizzotto  Márcia  Nora  Leonardo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(3):430-435

Plant Foods for Human Nutrition - The beneficial health effects of apple consumption are well known, however, little is known about the potential of its phenolic fractions to inhibit...  相似文献   

Agronomic Evaluation of Legume Cover Crops for Sustainable Agriculture     

Giliane?Aparecida?Vicente?da Silva Souza  Tancredo?Augusto?Feitosa?de SouzaEmail author  Djail?Santos  Elica?Santos?Rios  Gilmara?Juliana?de Lima Souza 《Russian Agricultural Sciences》2018,44(1):31-38

The agronomic characteristics of different legume cover crops and their effects on soil chemical properties were investigated in a short-term field study. We compared weed biomass, nitrogen equivalence, growth rate, leaf chlorophyll content, cover crop biomass, soil total organic carbon, and soil total nitrogen under eight different legume cover crops in a short-term field experiment. We found the highest growth rate, cover crop dry biomass, N plant content, and N contribution with C. ochroleuca plants, whereas for leaf chlorophyll content, soil organic carbon, and total nitrogen the highest values were found with M. pruriens. We did not find any significant difference among C. ochroleuca and M. pruriens for cover crop dry biomass. Our findings suggest that the incorporation of cover crops into the soil can change positively the soil chemical properties, such as soil organic carbon and total nitrogen. Our results also highlight the importance of considering the short-term effect of cover crops on the tropical soil fertility maintenance, in this case, Regosol.  相似文献   

Effects of live feed containing Panagrellus redivivus and water depth on growth of Betta splendens larvae          下载免费PDF全文

Márcia Valéria Silva do Couto  Natalino da Costa Sousa  Higo Andrade Abe  Joel Artur Rodrigues Dias  Juliana Oliveira Meneses  Peterson Emmanuel Guimarães Paixão  Fernanda dos Santos Cunha  Fabrício Menezes Ramos  Alexandre Nizio Maria  Paulo Cesar Falanghe Carneiro  Rodrigo Yudi Fujimoto 《Aquaculture Research》2018,49(8):2671-2675

Panagrellus redivivus is a nematode with a high protein content and low‐cost production. It is successfully used in the larviculture of shrimp, however, it has not yet been evaluated as feed for Betta splendens larvae. Thus, the objective of this study was to evaluate P. redivivus as feed for betta larvae maintained at different depths of water. The experiment was based on a completely randomized 3 × 2 factorial design, represented by two feed types and one alternating feeding regime plus two water depths (2.5 and 5.0 cm). The Artemia feed promoted the highest weight gain, followed by the alternating feeding regime. The single use of the nematode as live feed produced the poorest weight gain. The water depth and the feeding method influenced the specific growth rate (SGR). At a depth of 2.5 cm, the alternating feeding promoted similar SGR compared to fish fed with Artemia only. Furthermore, at the greater water depth an improvement in SGR was observed in fish fed Artemia only, but the similarity to alternating feeding regime remains. Thus, the inclusion of nematode is an appropriate and lower cost feed strategy for the betta larviculture independent of water depths.  相似文献   

Microbial biomass as an antioxidant for tilapia feed          下载免费PDF全文

Thiago Luís Magnani Grassi  Dayse Lícia Oliveira  Natália Mingues Paiva  Juliana Campos Pereira Diniz  Anelise Maria Bosco  Ariana Aparecida Ferreira Pereira  Amanda Regina Pinatti Menezes  Taiana Carvalho Valadares  Rosemeire Conceição Parra Pastor  Paulo César Ciarlini  Giovani Sampaio Gonçalves  Morris Villarroel  Elisa Helena Giglio Ponsano 《Aquaculture Research》2018,49(8):2881-2890

Microbial biomass (MB) produced by different industries is thought to be a beneficial supplement in fish feed due to high contents of antioxidants and pigments. However, little is known about their impact on fish health. In this experiment, 960 tilapia (26.84 ± 1.03 g) were fed one of eight experimental diets—a control diet with no MB (C), a control diet with vitamin E (VE) and six diets with three types of MB at two concentrations (0.25% and 0.5%): Rubrivivax gelatinosus (RG25 and RG50), Spirulina platensis (SP25 and SP50) and Saccharomyces cerevisiae (SC25 and SC50). Adding MB to diets decreased plasma total oxidant status, malonaldehyde and leucocyte respiratory burst; increased the total antioxidant status; and did not affect the blood biochemical parameters. In flesh, the use of the MB lowered the thiobarbituric acid‐reactive substances and increased redness (except for SC) and carotenoid deposition (except SC25). So, it was concluded that the use of the MB provided an antioxidant effect in tilapia blood plasma, decreased lipid oxidation and increased pigmentation and carotenoid deposition in the fish flesh, without imparting a negative impact on the animals’ health.  相似文献   

Micropropagation of <Emphasis Type="Italic">Eucalyptus grandis</Emphasis>  ×  <Emphasis Type="Italic">E. urophylla</Emphasis> AEC 224 clone     

Cassiana de Oliveira  Juliana Degenhardt-Goldbach  Gisela Manuela de França Bettencourt  Erika Amano  Luziane Franciscon  Marguerite Quoirin 《林业研究》2017,28(1):29-39

Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis  ×  E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 µM BA and 0.5 µM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 µM TDZ and 0.1 µM NAA during 30 days for callus induction and then with 5.0 µM BA and 0.5 µM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 µM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 µM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %.  相似文献   

Validation of the Italian version of the UNESP–Botucatu unidimensional composite pain scale for the assessment of postoperative pain in cattle     

Giorgia della Rocca  Juliana Tabarelli Brondani  Flávia Augusta de Oliveira  Martina Crociati  Lakamy Sylla  Agnes Elad Ngonput  Alessandra Di Salvo  Stelio Pacca Loureiro Luna 《Veterinary anaesthesia and analgesia》2017,44(5):1253-1261

Objective

The aim of this study was to validate the Italian version of the UNESP–Botucatu Unidimensional Composite Pain Scale (UCPS-IV) for assessing postoperative pain in cattle.

Study design

Video analysis and psychometric testing.

Animals

A total of 40 Nellore cattle, age 2–3 years, weighing 365 ± 51 kg.

Methods

An English version of the scale was translated to Italian and back translated to English to ensure semantic equivalence. A total of four Italian observers and the researcher who developed the original scale (considered as the gold standard) analysed videos of 40 cattle previously subjected to orchiectomy (recorded for each cattle at four predetermined timepoints during the perioperative period) unaware of the videos’ timepoint. They assigned a pain score [ranging from no pain (0) to severe pain (10)] using the Italian version of the scale and verified the need for analgesic treatment for each animal. The obtained scores were subjected to psychometric validity, responsiveness and reliability tests.

Results

The factor analysis of the scale resulted in a one-factor solution. Significant changes in pain scores in response to surgery and analgesics confirmed not only the content and construct validity, but also responsiveness. The agreement between the researcher who developed the original scale and the blinded observers and the correlation between the pain scores determined by the scale and those determined by three classical unidimensional pain scales confirmed criterion and concurrent validity. Internal consistency of the scale was excellent. Inter- and intrarater reliability ranged from moderate to good and from moderate to very good for all scale items, respectively, supporting reproducibility and stability. The cut-off point for rescue analgesia calculated by the receiver operating characteristic curve was > 3.

Conclusions and clinical relevance

The results confirm that the UNESP–Botucatu UCPS-IV appears to be a valid and reliable tool for the assessment of postorchiectomy pain in the bovine species. The determination of a cut-off point for rescue analgesia is also helpful for guiding analgesic therapy.  相似文献   

Predatory activity of the nematophagous fungus <Emphasis Type="Italic">Duddingtonia flagrans</Emphasis> on horse cyathostomin infective larvae     

Fabio R. Braga  Jackson V. Araújo  André. R. Silva  Rogério O. Carvalho  Juliana M. Araujo  Sebastião R. Ferreira  Laércio A. Benjamin 《Tropical animal health and production》2010,42(6):1161-1165

This work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L₃). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.000 L₃ and 1.000 conidia of the fungus. The control group without fungus only contained 1.000 L₃ in the plates. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for seven days under an optical microscope (10× and 40× objective lens) for non-predated L₃ counts. After 7 days, the non-predated L₃ were recovered from the Petri dishes using the Baermann method. The interaction there was a significant reduction (p < 0.01) of 93.64% in the cyathostomin L₃ recovered. The results showed that the D. flagrans is a potential candidate to the biological control of horse cyathostomin L₃.  相似文献