全文获取类型
收费全文 | 38859篇 |
免费 | 1448篇 |
国内免费 | 2441篇 |
专业分类
林业 | 5665篇 |
农学 | 3447篇 |
基础科学 | 1672篇 |
5555篇 | |
综合类 | 10049篇 |
农作物 | 3694篇 |
水产渔业 | 2992篇 |
畜牧兽医 | 4264篇 |
园艺 | 2459篇 |
植物保护 | 2951篇 |
出版年
2024年 | 105篇 |
2023年 | 359篇 |
2022年 | 806篇 |
2021年 | 900篇 |
2020年 | 874篇 |
2019年 | 868篇 |
2018年 | 3315篇 |
2017年 | 3690篇 |
2016年 | 1990篇 |
2015年 | 1214篇 |
2014年 | 1188篇 |
2013年 | 1405篇 |
2012年 | 2639篇 |
2011年 | 4064篇 |
2010年 | 3866篇 |
2009年 | 2881篇 |
2008年 | 2861篇 |
2007年 | 3049篇 |
2006年 | 1301篇 |
2005年 | 1071篇 |
2004年 | 762篇 |
2003年 | 677篇 |
2002年 | 693篇 |
2001年 | 571篇 |
2000年 | 489篇 |
1999年 | 244篇 |
1998年 | 158篇 |
1997年 | 133篇 |
1996年 | 74篇 |
1995年 | 89篇 |
1994年 | 95篇 |
1993年 | 70篇 |
1992年 | 58篇 |
1991年 | 51篇 |
1990年 | 29篇 |
1989年 | 24篇 |
1988年 | 33篇 |
1987年 | 14篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1962年 | 2篇 |
1956年 | 2篇 |
1932年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
961.
962.
963.
Soil enzymes are linked to microbial functions and nutrient cycling in forest ecosystems and are considered sensitive to soil
disturbances. We investigated the effects of severe soil compaction and whole-tree harvesting plus forest floor removal (referred
to as FFR below, compared with stem-only harvesting) on available N, microbial biomass C (MBC), microbial biomass N (MBN),
and microbial biomass P (MBP), and dehydrogenase, protease, and phosphatase activities in the forest floor and 0–10 cm mineral
soil in a boreal aspen (Populus tremuloides Michx.) forest soil near Dawson Creek, British Columbia, Canada. In the forest floor, no soil compaction effects were observed
for any of the soil microbial or enzyme activity parameters measured. In the mineral soil, compaction reduced available N,
MBP, and acid phosphatase by 53, 47, and 48%, respectively, when forest floor was intact, and protease and alkaline phosphatase
activities by 28 and 27%, respectively, regardless of FFR. Forest floor removal reduced available P, MBC, MBN, and protease
and alkaline phosphatase activities by 38, 46, 49, 25, and 45%, respectively, regardless of soil compaction, and available
N, MBP, and acid phosphatase activity by 52, 50, and 39%, respectively, in the noncompacted soil. Neither soil compaction
nor FFR affected dehydrogenase activities. Reductions in microbial biomass and protease and phosphatase activities after compaction
and FFR likely led to the reduced N and P availabilities in the soil. Our results indicate that microbial biomass and enzyme
activities were sensitive to soil compaction and FFR and that such disturbances had negative consequences for forest soil
N and P cycling and fertility. 相似文献
964.
We established protocols for the analysis of genetic diversity in chayote (Sechium edule) by using isozyme markers, thereby determining the level of genetic diversity present in 42 accessions of chayote from Costa
Rica. We obtained clear and reproducible zymograms for eight enzyme staining systems: PGM, 6-PGD, PGI, IDH, MDH, SOD, SKD,
and EST, and were able to score 14 putative loci. Eight of the 14 loci examined were polymorphic. We found 35 distinct multilocus
genotypes among these accessions. Five of these multilocus genotypes were homozygous for all loci. In addition, our data also
revealed that most of the multilocus genotypes (24) were heterozygous for only one of the eight loci, and the rest were heterozygous
for two or three loci (9 and 4 accessions, respectively). Seven multilocus genotypes were found in two different accessions.
Dice similarity coefficient was used to study the relationship between accessions. This analysis, based on the presence and
absence of alleles, revealed that accessions collected in the same location seldom shared the same multilocus genotype. The
value of isozyme polymorphisms as tools to continue studies on the characterization of chayote is discussed. 相似文献
965.
通过对加入外源铅的砂姜黑土、黄褐土、红壤进行培养处理,测定土壤中各形态铅及水溶态NH^+,K^+和速效态磷的含量,研究外源铅在各类土壤中的形态分布特点及对土壤养分的影响。结果表明:交换态和残渣态的铅为红壤〉黄褐土〉砂姜黑土,碳酸盐态铅为砂姜黑土〉黄褐土〉红壤,铁锰氧化态铅为红壤〉砂姜黑土〉黄褐土,有机结合态铅为黄褐土〉砂姜黑土〉红壤;交换态和残渣态所占比例随着外源铅的浓度增大而提高,有机结合态的比例随着外源铅浓度增大降低,碳酸盐结合态铅在砂姜黑土、铁锰氧化态铅在红壤上则呈现外源铅小于500mg/kg时比例增高,大于500mg/kg时比例开始下降的状况;加入外源铅的土壤中,水溶态的NH4^+和K^+的浓度增大,使其流失的风险性增加;水溶态磷和铅离子生成难溶性磷酸铅盐被固结,使速效磷的浓度减少,降低了水溶性磷肥的功效。 相似文献
966.
967.
968.
中慢生型天山根瘤菌胞外多糖相关基因exo5及其在根毛吸附和生物膜形成中作用的研究 总被引:1,自引:0,他引:1
用质粒pKGL 3上的转座子随机插入失活的方法得到两个中慢生型天山根瘤菌胞外多糖缺失菌株,并通过随机引物PCR的方法确定了转座子插入的基因位点,该基因与豌豆根瘤菌的UDP-葡萄糖脱氢酶基因有高度的同源性.根毛吸附实验发现该基因缺失,导致胞外多糖缺失突变菌株在其宿主植物甘草上的根毛吸附量大大降低,同时生物膜的形成实验发现中慢生型天山根瘤菌的exo5-菌株不能像野生型菌株一样形成丰富的生物膜,也从侧面证明了根毛吸附实验的结果,初步推测胞外多糖可能通过影响中慢生型天山根瘤菌的根毛吸附过程来影响其与豆科植物的共生固氮. 相似文献
969.
970.
Influence of non-cellulose structural carbohydrate composition on plant material decomposition in soil 总被引:1,自引:0,他引:1
Sophie Gunnarsson Håkan Marstorp A Sigrun Dahlin Ernst Witter 《Biology and Fertility of Soils》2008,45(1):27-36
The C mineralisation pattern during the early stage of decomposition of plant materials is largely determined by their content
of different carbohydrates. This study investigated whether detailed plant analysis could provide a better prediction of C
mineralisation during decomposition than proximate analysis [neutral detergent solution (NDF)/acid detergent solution (ADF)].
The detailed analysis included sugars, fructans, starch, pectin, cellulose, lignin and organic N. To determine whether differences
in decomposition rate were related to differences in hemicellulose composition, the analysis particularly emphasised the concentrations
of arabinose and xylose in hemicelluloses. Carbon dioxide evolution was monitored hourly in soil amended with ten different
plant materials. Principal component and regression analysis showed that C mineralisation during day 1 was closely related
to free sugars, fructans and soluble organic N components (R
2 = 0.83). The sum of non-cellulose structural carbohydrates (intermediate NDF/ADF fraction) was not related to C mineralisation
between days 1 and 9. In contrast, a model including starch and protein in addition to the non-cellulose structural carbohydrates,
with the hemicelluloses replaced by arabinose and xylose, showed a strong relationship with evolved CO2 (R
2 = 0.87). Carbon mineralisation between days 9 and 34 was better explained by xylan, cellulose and lignin (R
2 = 0.72) than by lignocellulose in the ADF fraction. Our results indicated that proximate analyses were not sufficient to
explain differences in decomposition. To predict C mineralisation from the range of plant materials studied, we propose a
minimum set of analyses comprising total N, free sugars, starch, arabinose, xylan, cellulose and lignin. 相似文献