油脂在提高公猪生产力和生育力上的潜在作用     

潘雪男  P.C.Penny  R.C.Noble  A.Maldjian  S.Cerolin 《国外畜牧学(猪与禽)》2001,(2):24-29

精子及其相关精浆中所含的脂肪成分在组成和脂肪酸成分上是非常独特的。长链多不饱和脂肪酸 (即具有 2 0或 2 2个碳原子并含三个以上双键的脂肪酸 )在绝大多数主要组织所含脂肪中的含量非常有限 ,但在精子和精浆中的含量相当高 ,并具有明显的种属特异性。油脂的这种特性现在日益被认为是使受精最终成功所特别需要的。本综述根据最近一的些研究结果撰写而成 ,从精液质量和功能的一系列决定因子的角度论述了使现代种公猪精子中脂肪 /脂肪酸参数最佳化的可能性 ,可见人们对一系列参数所作的一系列重要研究 ,产生了使公猪和母猪的繁殖能力都得到提高的高度有益结果。文章还从主要的营养学特性和化学特性方面对研究结果进行了讨论 ,并与其它家畜进行了比较 ,还讨论了其对猪繁育部门可能的商业应用价值。  相似文献   

Effect of folic acid supplements on homocysteine concentration in plasma of gestating sows.     

B Barkow  K Pietrzik  G Flachowsky 《Archives of Animal Nutrition》2001,54(1):81-85

An experiment comprising 19 German Landrace sows was established to evaluate the effect of folic acid supplements (10 mg/kg concentrate) on homocysteine and folic acid concentration in plasma and serum, respectively, of highly pregnant sows as compared to an unsupplemented control (basal diet contained 0.62 mg folic acid/kg concentrate). Blood samples were taken between day 75 and 110 of gestation for homocysteine analysis and on day 100 of gestation for folic acid determination. Due to the folic acid supplements serum folic acid concentration increased significantly (104 nmol/l in controls and 140 nmol/l in supplemented sows). In contrast, homocysteine concentration in the plasma was not significantly influenced by folic acid supplements (16.6 mumol/l in controls and 15.2 mumol/l in supplemented sows). Further investigations seem to be necessary to clarify the physiology of homocysteine metabolism in swine.  相似文献   

Concurrent cholangiocarcinoma, peritonitis, paratuberculosis, and aspergillosis in a goat.          下载免费PDF全文

M C Domínguez  G Chvez  F J Trigo    M L Rosales 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):884-885

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Use of an antineoepitope antibody for identification of type-II collagen degradation in equine articular cartilage.     

R C Billinghurst  E M Buxton  M G Edwards  M S McGraw  C W McIlwraith 《American journal of veterinary research》2001,62(7):1031-1039

OBJECTIVE: To develop an antibody that specifically recognizes collagenase-cleaved type-II collagen in equine articular cartilage. SAMPLE POPULATION: Cartilage specimens from horses euthanatized for problems unrelated to the musculoskeletal system. PROCEDURE: A peptide was synthesized representing the carboxy- (C-) terminus (neoepitope) of the equine type-II collagen fragment created by mammalian collagenases. This peptide was used to produce a polyclonal antibody, characterized by western analysis for reactivity to native and collagenase-cleaved equine collagens. The antibody was evaluated as an antineoepitope antibody by ELISA, using peptides +/- an amino acid at the C-terminus of the immunizing peptide. Collagen cleavage was assayed from equine articular cartilage cultured with interleukin-1 (IL-1), +/- a synthetic MMP inhibitor, BAY 12-9566. Cartilage specimens from osteoarthritic and nonarthritic joints were compared for antibody staining. RESULTS: An antibody, 234CEQ, recognized only collagenase-generated 3/4-length fragments of equine type-II collagen. This was a true antineoepitope antibody, as altering the C-terminus of the immunizing peptide significantly decreased competition for binding in an inhibition ELISA. The IL-1-induced release of type-II collagen fragments from articular cartilage was prevented with the MMP inhibitor. Cartilage from an osteoarthritic joint of a horse had increased staining with the 234CEQ antibody, compared with normal articular cartilage. CONCLUSIONS AND CLINICAL RELEVANCE: We generated an antineoepitope antibody recognizing collagenase-cleaved type-II collagen of horses. This antibody detects increases in type-II collagen cleavage in diseased equine articular cartilage. The 234CEQ antibody has the potential to aid in the early diagnosis of arthritis and to monitor treatment responses.  相似文献   

Use of laser photocoagulation for treatment of cutaneous angiomatosis in one dog and two cats.     

G M Peavy  E J Walder  J S Nelson  M Rosenberg 《Journal of the American Veterinary Medical Association》2001,219(8):1094-7, 1073

One dog and 2 cats were evaluated because of multiple progressively enlarging cutaneous vascular plaques. Biopsies were performed and revealed small well-circumscribed dermal nodules of dilated fully enclosed blood-filled spaces lined by single layers of endothelial cells aligned on thin fibrous walls, with minimal mitotic activity. A diagnosis of cutaneous angiomatosis was made in all 3 animals. Cutaneous angiomatosis is a progressive proliferative lesion of vascular tissue involving the dermis and subcutaneous tissues of dogs and cats. Lasers of selected wavelengths have been used to induce photothermal coagulation of hemoglobin-containing cutaneous lesions in humans; argon-pumped dye and neodymium:yttrium-aluminum-garnet lasers were used to treat the cutaneous lesions in the animals of this report. Laser treatment may provide an alternative to wide surgical resection or limb amputation for management of this condition in veterinary patients.  相似文献   

Association between Chlamydia psittaci seropositivity and abortion in Italian dairy cows.     

S Cavirani  C S Cabassi  G Donofrio  B De Iaco  S Taddei  C F Flammini 《Preventive veterinary medicine》2001,50(1-2):145-151

Although the seroprevalence of Chlamydia psittaci is widespread in Italian dairy herds, its role in inducing genital disorders has not been elucidated. We therefore set up a case-control study to compare seroprevalence to C. psittaci in an aborted-cow population and in a randomly selected control group in the province of Parma (the Po Valley of northern Italy). The true seroprevalence (45%) in aborted cows was significantly higher than that in the control group (24%) (adjusted odds ratio=2.53).  相似文献   

Immunohistochemical Detection of Mycoplasma agalactiae in Formalin‐Fixed,Paraffin‐Embedded Tissues from Naturally and Experimentally Infected Goats     

F. RODRÍGUEZ  G. A. RAMÍREZ  A. S. RAMÍREZ  H. J. BALL  A. ESPINOSA DE LOS MONTEROS  A. FERNNDEZ 《Zoonoses and public health》2002,49(5):226-229

Samples from the mammary tissue of 14 lactating goats (12 naturally infected and two experimentally infected) were examined for the presence of Mycoplasma agalactiae. A monoclonal antibody (5G12) was applied to formalin‐fixed, paraffin‐wax‐embedded sections and labelled by the avidin–biotin peroxidase complex (ABC) method. Histological examination of tissue sections revealed strong immunoreactivity in all animals included in the study. Mycoplasma agalactiae antigen was mainly detected in the cellular debris at the periphery of purulent exudates present within lactiferous sinuses, and lactiferous and interlobular ducts. In addition, M. agalactiae organisms appeared in the cytoplasm of the epithelium of ducts, and in infiltrating macrophages and neutrophils within the ducts, alveoli, interstitial tissue and regional lymph node sinuses. It is concluded that this monoclonal antibody‐based immunohistochemical technique is an efficient and specific method for the post‐mortem detection of M. agalactiae in cases of clinical mastitis as well as being a useful tool for the study of the route of infection and cellular types involved during mastitis caused by this organism.  相似文献   

关于饲料的胆碱含量     

H.A.Workel  黄晶 《国外畜牧学(猪与禽)》2002,(6):31-32

关于饲料中胆碱含量化学分析值的资料很多。饲料中的胆碱含量因饲料种类、出产国别、出产年份等的不同而不同。问题还不止于此，据发现，测定饲料中胆碱含量时所用的技术也不能给出充分可靠的结果，因而需要一种新技术。  相似文献   

Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns   总被引：1，自引：0，他引：1       下载免费PDF全文

Rocio Amezcua  Robert M. Friendship  Catherine E. Dewey  Carlton Gyles    John M. Fairbrother 《Canadian journal of veterinary research》2002,66(2):73-78

Post-weaning Escherichia coli diarrhea (PWECD) in Ontario was investigated using a case-control study involving 50 Ontario nurseries. The clinical signs and the impact on productive parameters were determined by means of a producer survey. The hemolytic E. coli serogroups involved in PWECD (O149:K91:K88) were examined in this study. Based on a polymerase chain reaction test, the hemolytic E. coli from 82% of the case herds were positive for 3 enterotoxins (STa, STb, and LT), those from 12% of the case herds were positive for STb and LT only, and those from one herd (6%) were positive for 3 enterotoxins, as well as for verotoxin and F18 pili. The E. coli involved in disease were resistant to multiple antibiotics. Case farms commonly used a wide variety of antibiotics either in the feed or water, or as injectable drugs. The most common antibiotic used to treat PWECD on the study farms was apramycin, but evidence of resistance to this antibiotic was noted. The PWECD problem was commonly seen within a week of weaning but onset of diarrhea was reported as late as the grower-finisher stage. Growth rate was poorer in case herds and mortality was higher than in control herds, demonstrating that PWECD is an economically important disease in Ontario.  相似文献   

Application and crop safety parameters for soil fumigants   总被引：2，自引：0，他引：2  

A. S. Csinos  T. M. Webster  D. R. Sumner  A. W. Johnson  C. C. Dowler  K. W. Seebold 《Crop Protection》2002,21(10):973-982

Metam sodium alone and in combination with 1,3-dichloropropene plus 17% chloropicrin (1,3-D+C-17) were evaluated under polyethylene mulch film as alternatives for methyl bromide in tobacco and tomato transplant production for both efficacy against pests and crop safety. Eight different weed species, 10 genera or species of fungi and several agronomic criteria were evaluated at three different sites. In general both the metam sodium alone and in combination with 1,3-D+C-17 were highly efficacious when compared to methyl bromide. Short polyethylene film retention times and short aeration times resulted in poor stands and poor crop vigor while relatively long polyethylene film retention times and long aeration periods at the same rates typically resulted in high stand counts and vigor. Combination treatments were more phytotoxic to germinating seed of tobacco and tomato. Vigor and stand counts of the seedlings were higher as aeration time increased, suggesting phytotoxic residues dissipate with time. Method of application of metam sodium, either injected with chisels or sprayed onto the soil surface and incorporated with a tractor-powered tiller alone or co- applied with 1,3-D+C-17 chisel injected, did not affect the efficacy of the treatments. Caution regarding phytotoxicity must be exercised when seeding into soil fumigated with metam sodium alone or combined with 1,3-D+C-17. Additional work will be required to establish safety periods required prior to transplanting crops into fumigated soil.  相似文献