Acknowledgment to Reviewers, 2007     

Tetsuro Okuno 《Journal of General Plant Pathology》2007,73(6):430-431

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F-wave conduction velocity, persistence, and amplitude for the tibial nerve in clinically normal cats   总被引：9，自引：0，他引：9  

Okuno S  Kobayashi T  Orito K 《American journal of veterinary research》2008,69(2):261-264

OBJECTIVE: To establish a method of F-wave evaluation and to determine normative values of F-wave parameters, including F-wave conduction velocity, persistence, and amplitude for the tibial nerve in cats. ANIMALS: 30 clinically normal cats. PROCEDURES: F-waves elicited in the interosseous muscles by stimulation of the tibial nerve were recorded, and linear regression analyses of the shortest latency versus the length of the tibial nerve and the limb length were performed. F-wave persistence was calculated by dividing the number of recorded F-waves by the number of stimuli. RESULTS: The correlation coefficient between F-wave latency and nerve length was 0.92, and that between F-wave latency and limb length was 0.58. Mean +/- SD F-wave conduction velocity of the tibial nerve was calculated to be 97.1 +/- 5.0 m/s. Linear regression analysis yielded the regression equation as follows: F-wave latency (milliseconds) = 2.60 + (0.02 x nerve length [mm]). Mean F-wave persistence and amplitude were 98.7 +/- 2.3% and 1.01 +/- 0.62 mV, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that nerve length should be used for nerve conduction studies of F-waves in felids. The regression equation for F-wave latency, conduction velocity, persistence, and amplitude may contribute to the diagnosis of nervous system diseases or injury in cats, such as trauma to the spinal cord or diabetic neuropathy.  相似文献   

Promoting Effects of Sucrose-rich Diet on N-Nitrosobis (2-oxopropyl) amine-induced Pancreatic Carcinogenesis in Hamsters     

Sakamoto A  Goya M  Degawa Y  Mitsui M  Mori T  Tamura K  Tsutsumi M 《Journal of toxicologic pathology》2010,23(1):19-24

It has been reported that there is an association between pancreatic cancer and obesity, impaired glucose metabolism and diabetes based on excess dietary fat and sugar intakes. A number of studies have suggested that a high-fat diet increases development of carcinomas in various organs and possible risk factors for pancreatic cancer. However, how an excess sugar intake promotes pancreatic carcinogenesis is still unknown. In the present study, we investigated the influence of an excess sugar intake on pancreatic carcinogenesis by administration of a sucrose-rich diet in which starch was replaced by sucrose in order to contain the same calories and other nutrients. Two similar experiments were performed. Six-week-old male Syrian golden hamsters were given N-nitrosobis (2-oxopropyl) amine (BOP) at a dose of 50 and 20 mg/kg body weight as a carcinogen in Week 0 and 1, respectively. In Week 2, the animals were divided into control and experimental groups. In experiment 1, 15 animals received a control diet or sucrose-rich diet in which 100% of the starch was replaced by sucrose, respectively. Since five animals fed on the sucrose-rich diet died by Week 12, the diet was changed to a sucrose-rich diet in which 50% of the starch was replaced by sucrose. In experiment 2, 15 animals received a control diet or sucrose-rich diet in which 50 or 20% of the starch was replaced by sucrose, respectively. All animals were sacrificed 25 weeks after the start of the experiment, and histological examination of the pancreas was performed. No significant difference was seen in the body weight at the end of the experiment. There were no significant differences in the glycosylated hemoglobin (HbA1c) and serum triglyceride, total cholesterol and HDL-cholesterol levels between the control and sucrose-rich diet groups in experiments 1 and 2. The incidence and number of carcinomas increased in hamsters fed the sucrose-rich diet compared with the control diet in experiments 1 and 2. These results suggest that an excess sucrose intake may promote the development of pancreatic cancer in hamsters.  相似文献   

N,N'-Bis(2-chloroethyl)-N-nitrosourea (BCNU)-induced Apoptosis of Neural Progenitor Cells in the Developing Fetal Rat Brain     

Yamaguchi T  Kanemitsu H  Yamamoto S  Komatsu M  Uemura H  Tamura K  Shirai T 《Journal of toxicologic pathology》2010,23(1):25-30

N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU) is one of the major drugs used in chemotherapy against malignant gliomas due to its effects, such as induction of bifunctional alkylation of DNA and formation of interstrand DNA cross-linkages, and induces cortical malformations in the fetal and neonatal rat brain. In this study, pregnant rats were treated with 7.5 mg/kg of BCNU on gestational day 13 (GD 13), and their fetuses were collected from 12 to 72 hours after BCNU treatment in order to examine the timecourses of morphological and immunohistochemical changes in neural progenitor cells in the developing brain. The number of pyknotic cells in the telencephalon peaked at 24 h and then gradually decreased until 72 h. The majority of these pyknotic cells were positive for cleaved caspase-3, a key executioner of apoptosis. The pyknotic cells showed the ultrastructural characteristics of apoptosis. The number of p53-positive cells began to increase prior to the appearance of apoptotic cells and p21-positive cells. The number of phosphorylated-histone H3-positive cells (mitotic cells) decreased from 24 to 36 h. The number of Iba1-positive cells (microglial cells) in the telencephalon increased from 12 to 48 h. These results suggest that BCNU induces p53-dependent apoptosis and reduces proliferative activity, resulting in reduction of the weight of the telencephalon and the thickness of the telencephalic wall in the fetal brain. This study will help to clarify the mechanisms of BCNU-induced fetal brain toxicity.  相似文献   

Anti-Influenza Virus Activity of Adlay Tea Components     

Nagai  Emiko  Iwai  Miwa  Koketsu  Ritsuko  Okuno  Yoshinobu  Suzuki  Yuri  Morimoto  Ryosuke  Sumitani  Hidenobu  Ohshima  Atsushi  Enomoto  Toshiki  Isegawa  Yuji 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(4):538-543

Plant Foods for Human Nutrition - Our previous study showed anti-influenza virus activity in adlay tea prepared from adlay seeds, naked barley seeds, soybean, and cassia seeds. In this study, we...  相似文献   

Accuracy comparison of cabbage coverage estimated from remotely sensed imagery using an unmixing method     

Kazuo Oki  Masaru Mizoguchi  Kosuke Noborio  Koshi Yoshida  Kazutoshi Osawa  Shoichi Mitsuishi  Tetsu Ito 《Computers and Electronics in Agriculture》2011,79(1):30-35

In this study, accuracy comparisons of the cabbage coverage estimated from AVNIR-2 and QuickBird imagery using an unmixing method were carried out to increase the amount of remote sensing data obtained at different levels of spatial resolution on different observation days. The accuracy rates of the cabbage coverage estimated using an unmixing method from AVNIR-2 and QuickBird imagery were almost the same. This result is very interesting, because it shows that we may be able to evaluate cabbage coverage using remote sensing data obtained at different spatial resolutions on different observation days. Thus, it would be possible to use different remote sensing imagery systems to evaluate cabbage conditions during the growing period using this proposed method.  相似文献   

First report of <Emphasis Type="Italic">Bean common mosaic virus</Emphasis> in yam bean [<Emphasis Type="Italic">Pachyrhizus erosus</Emphasis> (L.) Urban] in Indonesia     

Tri Asmira Damayanti  Desmiarti Susilo  Siti Nurlaelah  Dewi Sartiami  Tetsuro Okuno  Kazuyuki Mise 《Journal of General Plant Pathology》2008,74(6):438-442

Severe mosaic with leaf malformation and green vein banding was observed on yam bean in West and Central Java, Indonesia. Virions of the causal virus were flexuous filaments, about 700 nm in length, with a coat protein of 30 kDa. The virus was transmitted by mechanical inoculation and by aphids in a nonpersistent manner. The nucleotide sequence of the coat protein gene had the highest identity with that of Bean common mosaic virus (BCMV, genus Potyvirus) isolate VN/BB2-5. Based on demarcation criteria, including the genome sequence and host range, we tentatively designate this isolate as BCMV-IYbn (Indonesian yam bean). The nucleotide sequence reported is available in the DDBJ/EMBL/GenBank databases under accession number AB289438.  相似文献   

Soil properties affecting adsorption of plasmid DNA and its transformation efficiency in Escherichia coli     

Takashi Kunito  Yasutaka Ihyo  Hiromu Miyahara  Ryo Seta  Seishi Yoshida  Hiroyoshi Kubo  Kazunari Nagaoka  Masao Sakai  Kazutoshi Saeki 《Biology and Fertility of Soils》2016,52(2):223-231

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Porencephaly with an optic organ abnormality in a beagle dog     

Osamu Hashiguchi  Yuko Yamaguchi  Shinichiro Ikezaki  Tsubasa Saito  Saori Igura  Ryo Hirai  Naomi Horiuchi  Kazutoshi Tamura 《Journal of toxicologic pathology》2022,35(1):107

A female TOYO beagle dog showed porencephaly and visual organ abnormalities. At necropsy, there was a cavity filled with cerebrospinal fluid in the right cerebral hemisphere and an adhesion area between the cerebral cortex and the skull, which was partially thickened. Additionally, the right optic nerve showed a slight decrease in diameter. Histopathological examination revealed increased glial fibers and collagen fibers, hemosiderin deposition, and an increased number of microglia in the adhesion area, along with a marked reduction of the cerebral parenchyma. In the right eyeball, the retina and optic nerve showed focal atrophy in the nerve fiber layer and inner granular layer to full retinal atrophy and hypoplasia of the myelinated nerve fibers, respectively. Electron microscopic examination revealed hypoplasia of the myelin sheath of nerve fibers in the right optic nerve. This is an extremely rare case of porencephaly and congenital optic nerve hypoplasia, along with independent retinal thinning.  相似文献   

Spontaneous Renal Tumors Suspected of Being Familial in Sprague-Dawley Rats     

Kayoko Kudo  Toru Hoshiya  Tomomi Nakazawa  Tsubasa Saito  Natsumi Shimoyama  Isamu Suzuki  Kazutoshi Tamura  John Curtis Seely 《Journal of toxicologic pathology》2012,25(4):277-280

Spontaneous renal tubule tumors (RTTs), with a distinctive morphological phenotype, were present in three Sprague-Dawley rats, 1 male and 2 females, out a total of 120 animals of each sex from untreated and placebo control groups in a 2-year carcinogenicity study. One female had one carcinoma, adenoma and hyperplasia, and the other female had five adenomas and many hyperplastic lesions; the male case had one carcinoma. From these cases, a biological continuum of hyperplasia, adenoma and carcinoma could be recognized. The tumors were present in the renal cortex and appeared as solid lobulated growths with occasional central necrosis. The lobules were divided by a small amount of fibrovascular tissue. Occasionally the larger tumors contained a cystic area. Tumor cells appeared distinctive and exhibited variable amounts of eosinophilic/amphophilic and vacuolated cytoplasm. Nuclei were round to oval with a prominent nucleolus. Mitotic figures were uncommon, and no distant metastasis was noted. The tumors were seen as multiple and bilateral lesions in two animals and had no apparent relationship to chronic progressive nephropathy (CPN). Foci of tubule hyperplasia were also noted to contain the same type of cellular morphology. The morphological and biological features of these 3 cases resembled the amphophilic-vacuolar (AV) variant of RTT that has been posited to be of familial origin. This is a report of spontaneous familial renal tumors in Sprague-Dawley rats from Japan.  相似文献