Evaluation of biocontrol agents for managing root diseases on hydroponically grown tomato     

Khalil  S.  Alsanius  B. W. 《植物病害和植物保护杂志》2010,117(5):214-219

Journal of Plant Diseases and Protection - The effect of the bacterial strain Pseudomonas fluorescens 5.014 together with three commercial biocontrol products (Binab T, Gliomix and Mycostop)...  相似文献   

Nigella sativa seed protects against 4‐nonylphenol‐induced haematotoxicity in Clarias gariepinus (Burchell, 1822): Oxidant/antioxidant rebalance          下载免费PDF全文

N.S. Abou Khalil  M. Abd‐Elkareem  A.H. Sayed 《Aquaculture Nutrition》2017,23(6):1467-1474

4‐Nonylphenol (NP) is an emerging concern contaminant which is widely spread in the aquatic ecosystem. Nigella sativa seed (NSS) has multifaceted therapeutic values. This study aimed to give insight into the potential protective effect of NSS on NP‐induced haematotoxicity in Clarias gariepinus through evaluation of haematological parameters, oxidant/antioxidant balance of blood lysate and histopathological investigation of blood smear. One hundred and fifty fish were divided into five groups (30/group). First group served as control which did not received NP exposure and fed basal diet without NSS supplementation. The other four groups were exposed to NP at a dose of 0.1 mg L^?1 and fed diets supplemented with NSS at levels of 0, 10, 25 and 50 g/kg diet, respectively. Macrocytic hypochromic anaemia, thrombocytopenia, leucopenia, neutrophilia, lymphopenia, monocytosis and eosinophilia were observed following NP exposure together with increase in morphological erythrocyte alterations and micronuclei formation. Elevation in total peroxide and malondialdehyde and depletion in total antioxidant capacity of blood lysate were reported. We concluded that supplementation of NSS markedly ameliorated the previously listed manifestations, and the most effective doses were 25 and 50 g/kg feed.  相似文献   

Characterization and Quantification of Native Glutenin Aggregates by Multistacking Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Procedures     

Dong Yin Huang  Khalil Khan 《Cereal Chemistry》1997,74(3):229-234

Native glutenin aggregates of two different quality flours containing the same high molecular weight (HMW) glutenin subunit compositions were investigated by multistacking sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) procedures. Five stacking gels (4, 6, 8, 10, and 12%) with a separating (resolving) gel of 14% were used to separate nonreduced glutenin aggregates solubilized from flour by SDS sodium phosphate buffer. There were large differences in protein solubility of the two flours. It took 8 hr to extract 91% of total proteins from the strong flour (variety Len) while it took only 2 hr for the weak flour sample 205. Total glutenin proteins and the proportions of glutenins at the different origins (including the origin of the 14% separating gel) were quantified by high-resolution densitometry procedures. As the duration of extraction increased, both total glutenins and glutenins at the 4% origins increased. The good quality flour Len had a higher total glutenin protein (3% more) and higher proportion of glutenins with the largest molecular sizes (also 3% more) at the 4% origins than the poor quality flour sample 205. After glutenin aggregates from each origin were reduced and analyzed by SDS-PAGE, the largest glutenins at the 4% origin contained twice the amount of total HMW glutenin subunits when compared to the smaller aggregates at the 12% origin. Among the total HMW glutenin subunits, the proportion of subunit 5 (which published literature reports to be the largest molecular weight based on calculations of DNA-derived amino acid sequence analysis) was twice that at the 12% origin. A randomized structure of native glutenins is proposed based on the results of our investigations.  相似文献   

Partial purification and characterization of a protein in porcine follicular fluid which restricts sperm-egg interaction in vitro.          下载免费PDF全文

J Ramsoondar  W Khalil    B R Downey 《Canadian journal of veterinary research》1995,59(1):8-14

An attempt was made to isolate and characterize a component in preovulatory porcine follicular fluid (pFF) which has a restricting effect on sperm-egg interaction in vitro. Using the zona-free hamster ova (eggs) penetration assay as an in vitro test system, it was shown previously that the numbers of porcine spermatozoa attached to or penetrated into each egg and the number of eggs with sperm attached or penetrated decreased significantly as the concentration of pFF was increased in the culture medium. In the present study, the component in pFF having these effects was shown to be a heat stable, nonsteroidal substance which retained its activity after dialysis, lyophilization and gel filtration chromatography. The activity was also found to be present in preovulatory homologous serum. Separation of the material on protein type gel filtration columns with detection at 280 nm, together with the banding seen with Coomassie staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), suggests that it is a protein. Based on high pressure liquid chromatographic separation (HPLC) and SDS-PAGE analyses, the bioactivity could be due to a single protein of 87 kD or to one or more of three smaller proteins, possibly disaggregated products of the 87 kD protein, in the range of 26-28 kD.  相似文献   

Thelazia lacrymalis in the eyelids of British horses     

J B Arbuckle  L F Khalil 《The Veterinary record》1978,103(8):158-159

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Cholecystitis in otters (Lutra lutra) and mink (Mustela vison) caused by the fluke Pseudamphistomum truncatum     

Simpson VR  Gibbons LM  Khalil LF  Williams JL 《The Veterinary record》2005,157(2):49-52

Between 1988 and 2004, postmortem examinations were carried out on 445 otters found dead, mostly as a result of road traffic accidents, in southern and south-west England. Thickened, shrunken gall bladders were observed in 10 cases, the first in 2000 and the others between February 2002 and August 2004. A digenean fluke, Pseudamphistomum truncatum, was found in the gall bladders of three cases and also in three of seven American mink examined. Nine of the 10 otters and all the mink came from a localised area of Somerset, indicating that the fluke has become established in the local fish population. P. truncatum has not been recorded previously in Britain, and the results suggest that it has been introduced recently, possibly in imported fish.  相似文献   

Crossbreeding effects for litter and lactation traits in a Saudi project to develop new lines of rabbits suitable for hot climates   总被引：1，自引：1，他引：0  

A.M. Al-Saef  M.H. Khalil  A.H. Al-Homidan  S.N. Al-Dobaib  K.A. Al-Sobayil  M.L. García  M. Baselga 《Livestock Science》2008,118(3):238-246

A five-years crossing scheme involving the Spanish V line (V) and Saudi Gabali (S) rabbits was practiced to produce 14 genetic groups: V, S, 1/2V1/2S, 1/2S1/2V, 3/4V1/4S, 3/4S1/4V, (1/2V1/2S)², (1/2S1/2V)², (3/4V1/4S)², (3/4S1/4V)², ((3/4V1/4S)²)², ((3/4S1/4V)²)², Saudi 2 (a new synthetic line) and Saudi 3 (another new synthetic line). A total of 3496 litters from 1022 dams were used to evaluate litter size at birth (LSB) and weaning (LSW), litter weight at birth (LWB), litter weight at 21 d (LW21) and litter weight at weaning (LWW), pre-weaning litter mortality (PLM), milk yield at lactation intervals of 0–7 d (MY07), 0–21 d (MY021), 0–28 d (TMY) and milk conversion ratio as g of litter gain per g of milk suckled during 21 d of lactation (MCR021). A generalized least squares procedure was used to estimate additive and heterotic effects (direct, maternal, and grand-maternal).The comparison among V, S, Saudi 2 and Saudi 3 showed a complementarity between V and S. Line V was superior for LSB, LSW, LWB, PLM, MY07, MY021 and TMY, while line S was superior for the other traits (LW21, LWW and MCR021). Saudi 2 and Saudi 3 had the means equal to or higher than the founder lines (V or S) for all traits. Saudi 2 showed better values in litter size and pre-weaning litter mortality compared to Saudi 3 with no significant differences for the other traits. Concerning crossbreeding parameters, direct additive effects were significant for all traits, ranging between 12.3% and 31.8% relative to the average of the means of V and S. All estimates for direct heterosis except LWB and MCR021 were significant and ranged from 5.3% to 27.5%. No estimates for maternal additive effects and grand-maternal additive and heterotic effects were significant. Only estimates for maternal heterotic effects of LSB and LSW were significant (8.6% and 10.6%, respectively).  相似文献   

Effect of different dietary zinc sources on seminal plasma enzymatic activity,antioxidant, and immune-related gene expression in rainbow trout (Oncorhynchus mykiss)     

Kazemi  Esmaeil  Nazari  Sajad  Sourinejad  Iman  Pourkazemi  Mohammad  Paknejad  Hamed  Eslamloo  Khalil 《Aquaculture International》2021,29(6):2731-2750

Aquaculture International - The objective of the present work was to survey the effects of different sources of zinc supplements on serum enzymatic activities, the expression of genes related to...  相似文献   

Physiological and molecular evaluation of ten Egyptian barley cultivars under salt stress conditions     

Khalil  Shaimaa R. M.  Ashoub  Ahmed  Hussein  Basita A.  Brüggemann  Wolfgang  Hussein  Ebtissam H. A.  Tawfik  Mohamed S. 《Journal of Crop Science and Biotechnology》2022,25(1):91-101

Journal of Crop Science and Biotechnology - Ten Egyptian barley cultivars (Giza 129, 132, 123, 127, 2000, 130, 126, 135, 128 and 133) were assessed under 0, 100 and 200 mM NaCl. The...  相似文献   

Antiinflammatory activity and acute toxicity of Dodonaea viscosa     

Khalil NM  Sperotto JS  Manfron MP 《Fitoterapia》2006,77(6):478-480

The hydroalcoholic extract (HAE) of the leaves of Dodonaea viscosa, given by oral route at dose of 300 mg/kg, significantly inhibited the paw edema induced by carrageenin injection. The extract did not show any sign of toxicity in mice up to 5000 mg/kg p.o. This result seems to support the use of D. viscosa leaves ethanolic extract in relieving inflammation.  相似文献