Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress     

Wang Junjuan  Lu Xuke  Yin Zujun  Wang Delong  Wang Shuai  Mu Min  Chen Xiugui  Guo Lixue  Fan Weili  Chen Chao  Ye Wuwei 《棉花学报》2019,31(2):89-100

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.  相似文献   

餐厨废弃物作堆肥对盆栽番茄产量及品质影响   总被引：1，自引：0，他引：1  

杨丽娟  李天来  储慧霞  王艳  杨黾  李忠元 《沈阳农业大学学报》2010,41(6)

采用盆栽试验以常规量化肥(CF)、不施肥(CK)为对照,研究了餐厨废弃物(FW)、鸡粪(CM)、猪粪(PM)、马粪(HM)、牛粪(BM)等有机肥对番茄生长发育、产量及果实品质的影响。结果表明:餐厨废弃物促进番茄植株生长发育的效果优于其他有机肥处理。餐厨废弃物、猪粪、牛粪、马粪、化肥、鸡粪、对照各处理番茄的产量分别为863.6 g.pot-1,825.0 g.pot-1,816.3 g.pot-1,766.9 g.pot-1,672.0 g.pot-1,659.1 g.pot-1,616.7 g.pot-1。番茄地上部分生物量变化次序为餐厨废弃物猪粪牛粪马粪鸡粪化肥对照。餐厨废弃物处理番茄Vc、可溶性糖含量显著高于其他处理,Vc含量分别比牛粪、猪粪、鸡粪、化肥、对照处理提高7.0%、10.0%、14.4%、14.8%和18.2%;可溶性糖含量分别比猪粪、鸡粪、化肥、对照处理提高9.7%、11.6%、19.3%和60.0%;餐厨废弃物处理番茄糖酸比为7.73,显著高于其他处理。因此,餐厨废弃物能促进番茄生长发育,提高番茄产量与品质,在蔬菜生产中可以作堆肥替代部分化肥与其他动物性有机肥,具有广泛应用前景。  相似文献   

复方中草药对镜鲤(Cyprinus carpio var.specularis)幼鱼非特异性免疫功能的影响     

孟兆娜  陈玉春  刘敏 《东北农业大学学报》2011,42(3):54-60

在基础日粮(对照组)中分别添加2%由不同比例的刺五加(Acanthopanax senticosus Harms)、枸杞子(L.Chinense Mill.)、金银花(Lonicera japonica Thunb)和黄芪(Astragalus membranaceus)配伍的复方中草药,记为复方Ⅰ、复方Ⅱ、复方Ⅲ、复...  相似文献   

NaCl胁迫下构树离子相对含量及分布的能谱分析     

张敏  黄利斌  王磊 《西南林学院学报》2011,31(1):17-21

应用扫描电镜和X射线能谱仪测定不同浓度NaCl胁迫下构树幼苗根、茎、叶3器官中离子的相对含量,并对其组织分布特征进行分析。结果表明：盐胁迫下构树幼苗各器官中Na^＋和Cl^-相对含量均高于对照,K^＋、Ca^2＋和Mg^2＋相对含量则低于对照;随着盐处理浓度的增加,根部皮层和髓细胞中的Na^＋和Cl^-相对含量增幅较高,茎中的Na^＋和Cl^-主要积累于表皮细胞,皮层中分布较少,叶中Na^＋和Cl^-在表皮细胞中积累较多,而栅栏组织和海绵组织中含量相对较少;在高盐胁迫下,地上部分Ca^2＋含量明显高于根部;NaCl胁迫下构树幼苗不同器官K^＋含量下降的幅度不同,根和茎中K^＋含量均降低,而叶片中K^＋含量与对照相比变化不明显,在组织水平上,K^＋在海绵组织和栅栏组织中的相对含量有所增加;盐胁迫对P^3＋含量影响较小,其分布特点是主要积累于根部。  相似文献   

Phylogeny of Limenitidinae Butterflies （Lepidoptera： Nymphalidae） Inferred from Mitochondrial Cytochrome Oxidase I Gene Sequences     

ZHANG Min  CAO Tian-wen  ZHONG Yang  GUO Ya-ping  MA En-bo 《中国农业科学(英文版)》2011,10(4):566-575

The phylogenetic analyses of the subfamily Limenitidinae are performed based on 1471 bp of mtDNA cytochrome oxidase subunit I (C01) gene sequence data which were obtained from 21 individuals spanning 9 genera, along with those of 17species obtained from GenBank, using Apatura iris, Aglais urticae, and Polyura dolon as outgroup species. Although the transitions at the third codon positions of the COI data set were highly saturated, they were still retained for analysis as they contain the majority of the phylogenetic information, and thus, the maximum pasimony (MP) under different weighting schemes and maximum likelihood (ML) trees were reconstructed in this study. The results showed that within this subfamily, the results based on the COI gene sequences are approximately identical to the traditional classification results. However, the clustering of Lexias pardalis and Tanaecia julii within the genus Euthalia as well as the clustering of Phaedyma aspasia within the genus Neptis with weak support are different from that of the current classification scheme made by Chinese scholars. The genus Limenitis is splited into two subelusters in the trees constructed by using MP and ML methods. These results support one of the strongest hypotheses for the tribe relationships within Limenitidinae.  相似文献   

山地丘陵耕地土壤养分最优插值方法研究——以江西省渝水区水北镇为例   总被引：2，自引：0，他引：2  

郭熙  黄俊  谢文  陶丹丹  鲁敏  赵小敏 《河南农业科学》2011,40(2):76-80

以江西省渝水区水北镇为研究区,利用823个采样点的耕地土壤养分数据,分析区内耕地土壤养分含量与海拔、坡度的相关性,采用协同克里格插值方法,结合海拔、坡度进行插值,并把经交叉检验后的结果与逆距离权重法、普通克里格法的结果进行比较.结果表明,结合海拔、坡度进行插值后,精度有明显提高.插值精度排序为协同克里格法>普通克里格法...  相似文献   

海藻酸钠包埋固定胆红素氧化酶的优化     

郑文爽  赵敏 《东北林业大学学报》2011,39(3):127-128

为改善胆红素氧化酶性质,提高酶的使用效率,以海藻酸钠为载体,采用包埋法固定了胆红素氧化酶,并优化了固定化条件,研究了固定化胆红素氧化酶的酶学性质.结果表明:海藻酸钠的质量浓度为0.10 g·mL-1、CaCl,浓度为0.20 mol·L-1、固定时间为1 h、酶的固定化效果最好,固定化酶的平均酶活力回收率达32%,固定...  相似文献   

重庆市南岸区环境友好型土地利用模式研究     

彭敏  廖和平  曾璐  魏洪斌  叶茂 《现代农业科技》2011,(1):285-286,288

为协调重庆市南岸区土地利用与生态环境之间的关系,构建生态友好型土地利用模式,促进土地资源可持续利用,应用文献资料法,对南岸区土地资源利用数量特征、空间布局、利用特点进行分析,综合分析评价了南岸区不同土地利用类型对生态环境的影响,得出南岸区都市生态休闲农业模式、环境友好型城镇土地利用模式、工业土地利用环境友好模式。提出了土地利用与生态环境建设的措施,以期在发展经济的同时建设生态环境,实现土地利用与生态环境协调发展。  相似文献   

加强农业科研档案管理 提高农业科研管理水平   总被引：7，自引：9，他引：7  

梅盈洁  邱俊荣  刘军  尹艳  张大彤  汪敏  程俊峰 《农业科技管理》2011,30(2):14-17

文章从分析农业科研档案管理工作的重要作用入手,指出了目前我国农业科研院所在科研档案管理中存在的问题与不足,在借鉴国内外科研档案管理工作先进经验的基础上,提出了更新思想观念,提高科研档案管理意识;落实和完善科技档案规章制度,依法抓好档案管理工作;创新工作方式,提高工作效率;抓住项目重要节点,加强科研档案收集;加强人才培养,提高业务素质等对策。  相似文献   

桃李杏资源亲缘关系的ISSR分析   总被引：1，自引：0，他引：1  

梁敏  董文轩  甄睿  高书燕  赵恺  邓继光  张景娥 《贵州农业科学》2011,39(5)

为了解桃、李、杏属间及各属的品种间亲缘关系,给育种提供新的分子依据,同时为科学合理地保存和利用桃、李、杏种质资源提供科学依据,对47份桃、李、杏资源及7个桃李杂交后代株系的亲缘关系进行了ISSR分析.结果表明:筛选出的7条引物共扩增出103条谱带,每个引物扩增的条带数在11～22条.UPGMA聚类结果表明,54份资源按照桃、李、杏分为3组,其中杏位于桃、李之间;7个杂交株系聚在了桃组内.ISSR技术可以很好地用于桃、李、杏亲缘关系的研究.  相似文献