藏猪与杜洛克猪血液中胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3含量差异研究     

陶璇  顾以韧  梁艳  杨雪梅  曾凯  陈晓晖  钟志君  杨跃奎  吕学斌 《养猪》2015,(2):65-67

为了解国内、外两类猪种肌肉生长发育及血清中相关细胞因子含量差异,以我国特有的藏猪和国外杜洛克猪作为研究对象,采用酶联免疫分析法(ELISA)测定6月龄藏猪、杜洛克猪血清中胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)的含量及肌纤维面积(CSA)。结果显示:藏猪血清中IGF-1含量及CSA均极显著低于杜洛克猪(P0.01),IGFBP-3含量极显著高于杜洛克猪(P0.01);CSA与IGF-1含量间呈极显著正相关,与IGFBP-3间呈极显著负相关,说明IGF-1对猪背最长肌生长发育有促进作用,而IGFBP-3可能有抑制作用。  相似文献   

安吉白茶对柱状黄杆菌抑菌机理的研究     

吕娜  韦薇  顾帅  庞超  李雪娇  郭畅 《中国畜牧兽医》2015,42(1):136-139

为探讨安吉白茶对柱状黄杆菌的抑菌机理,本试验通过测定安吉白茶提取液与细菌作用前后培养液电导率和紫外吸收物的变化,以及菌体磷代谢和可溶糖的变化,初步阐明了安吉白茶对柱状黄杆菌的抑菌机理。研究结果显示,经安吉白茶提取液处理后,细菌培养液的电导率和可溶糖浓度均增大,菌悬液中的紫外吸收物也随作用时间的延长而增加,表明安吉白茶提取液可破坏细胞膜的结构、导致细胞通透性增加,进而使细胞内容物外泄。此外,经安吉白茶处理后的柱状黄杆菌对磷的消耗量降低,以致严重影响了核酸、磷脂等细胞重要成分的合成及能量代谢,导致细菌正常生理功能的丧失。结果表明,安吉白茶可通过破坏菌体细胞膜及干扰磷代谢等途径抑制柱状黄杆菌的生长。  相似文献   

Pathogenicity of a Strain of H5N6 Subtype Avian Influenza Virus in Ducks and Mice     

LIU Yong-fa  HUANG Yu-mei  ZHANG Zong-yao  WAN Hong  WANG Mei  LV Jia-min  LUO Kai-jian 《中国畜牧兽医》2015,42(8):2176-2182

To investigate the pathogenicity of A/Hero/Guangdong/C1/2013(H5N6), an AIV strain isolated from Heron on ducks and mice, pathogenicity of this new virus strain and changing of histopathology as well as a preliminary study on its biological characteristics were studied by virus challenges test via intranasal and eye-drop and in chicken via jugular vein injection.Results showed that the EID₅₀ of this strain of virus was 10^-8.16/0.1 mL in embryonated chicken eggs and the intravenous inoculation of pathogenic index (IVPI) was 2.76.In ducks and mice, the 50% lethal doses (LD₅₀) of it were determined to be 10^-4.0/0.2 mL and 10^-4.67/0.05 mL, respectively.Symptoms of infection included loss of appetite, depression, swollen head and tears after being infected with 10⁶ EID₅₀ per duck by intranasal and eye-drop administration.Most of ducks died 4 to 7 days post-infection, liver, lung and kidney still eliminated toxicants at day 7 post-infection.Anatomy showed symptoms of pericardial effusion, pulmonary congestion and kidney enlargement, while pathological section showed pathological change like karyopycnosis and inflammatory cell infiltration in heart, liver, kidney and spleen.Mice developed symptoms of infection like loss of appetite, depression, shaggy coat and ruffled coat after being infected with 5×10⁵ EID₅₀ per mouse by intranasal and eye-drop administration.Most of the infected mice died 5 to 7 days post-infection and only liver still eliminated toxicants at day 7 post-infection.Although organ anatomy showed no obvious pathological changes, pathological section showed pathological changes like karyopycnosis and inflammatory cell infiltration in heart, kidney and lung.Our research demonstrated that this H5N6 subtype AIV had a strong pathogenicity and could be defined as a highly pathogenic AIV strain as its IVPI was greater than 1.2.Our work laid a theory foundation for study, prevention and control of H5N6 subtype AIV.  相似文献   

Effect of Heat Shock Protein-90 (HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix     

HOU Ning  LV Zhi-hui  ZHAO Liang  GAO Xue-li  LIU Chao-nan  LV Xiao-ping  ZHENG Shi-min 《中国畜牧兽医》2015,42(8):2183-2188

The 90 chickens were randomly divided into control group, E.necatrix one time infection group and E.necatrix two times infection group.We used duplex PCR method to test the expression changes of HSP-90 mRNA in intestinal tissue of chickens infected with E.necatrix and researched the effects of it comprehensively and systematically.The results were showed that after 0 to 14 days the expression of HSP-90 mRNA in intestinal tissues of 14-day-old chickens infected with E.necatrix presented a upward trend though it was lower than the control chickens.These chickens were infected with E.necatrix again at 28 days, HSP-90 mRNA expressions of duodenum, jejunum and ileum decreased and then increased.However, it always showed a downward trend in appendix.When 28 days chickens were infected with high-does E.necatrix once, HSP-90 mRNA expressions of duodenum, jejunum and appendix were in a declining curve.While it was increased originally and then decreased in ileum.This study indicated that HSP-90 mRNA expressions in intestinal tissue of chickens infected with E.necatrix were inhibited at first, and the expression quantity was closely related to the degrees of intestinal tissue damage.  相似文献   

濒危植物艾比湖桦生存的水文地质及土壤条件     

孙建军  丁祺仁  吕锦萍  李秀珍  李霞飞 《土壤》2007,39(5):840-843

初步研究发现,艾比湖桦仅见于古尔图河古老三角洲西缘泉水溢出地带.虽地处温带漠境,但土壤却系既无盐化又无碱化且碳酸钙含量很低的草甸沼泽土,地下水矿化度小于1g/L,pH值6.9～7.4.  相似文献   

气候变化对桂花分布的影响研究     

武耀星      张梅    杨勇    吕泽民  张秀卿  王亮生   《西北林学院学报》2022,37(4):129-134

桂花是我国特有树种,具有很高的经济价值而被广泛栽培,主要用于园林绿化、饮食和药用,桂花的栽培品种在城乡园林景观中较为多见,但野生资源破坏严重,生境恶化,物种多样性也随之减少。该文在不同气候情景下,对桂花的潜在适生区分布进行预测,可探究气候变化下该物种的生境变迁,并了解其地理分布与气候间的相互关系,为桂花的资源保护与利用提供重要的科学依据。利用最大熵（MaxEnt）模型,根据影响桂花适生区分布的31个环境因子和当前桂花生境的368个自然分布点,对当前和未来（2050年、2070年）桂花的潜在分布区进行预测,并用刀切法检验来评估气候因子的重要性。结果表明:1）在当前环境条件下,桂花在我国主要分布在33°N以南的地区,总潜在分布区面积约224.23×10⁴ km²;2）干燥季节的月降水量、潮湿的频率、干旱季节的平均温度是影响桂花生境的主导环境因子;3）随着未来气候的改变,桂花的潜在分布区向北偏东高纬度地区发生迁移,西南地区潜在分布区减少,建议该地区的桂花居群优先保护。  相似文献   

楚雄华山松种子园无性系幼苗叶枯病病原菌鉴定     

吕则佳  陈健鑫  冯峻  朱艳  董云祥  罗正平  马焕成  伍建榕   《西北林学院学报》2022,37(4):181-187

为明确楚雄市华山松种子园无性系幼苗叶枯病的病原,采用传统形态学和分子生物学相结合的方法对华山松叶枯病进行病原鉴定。结果表明,引起华山松叶枯病的病原菌为胡枝子拟盘多毛孢（Pestalotiopsis lespedezae）和互隔链格孢（Alternaria alternata）。  相似文献   

绣球菌菌丝体液态发酵培养工艺条件优化     

王斌  吕晗琪  张腾霄  桑亚姣  张春华  李爽 《陕西农业科学》2022,(6):89-92

为绣球菌进一步开发利用及工厂化生产奠定基础,在单因素实验基础上采用正交试验分析法,以绣球菌菌丝鲜重为指标,确定最佳工艺参数。结果表明：绣球菌菌丝体液态发酵最佳工艺条件：PDA培养基、培养温度26℃、振荡速度150 r/min、发酵时间13 d、碳源为葡萄糖、氮源为玉米粉。以液态发酵法高效获取绣球菌菌丝体及菌种是行业规模化发展的必然需求,本研究结果为绣球菌后续相关研究提供了理论依据。  相似文献   

蔬果合作社农产品品牌化与农户农产品生产质量控制行为——影响机制与效应测度     

张益丰  吕成成  陆泉志 《中国农业大学学报》2022,27(7):283-298

为探索参与农民合作社与提升农户实施农产品质量控制行为之间的内在关联,基于山东省985户蔬果种植类农户调研数据,在采用层次分析法(AHP)测度农产品质量控制行为效能基础上,运用内生转换模型、中介效应模型分析合作社促进农户实施农产品质量控制行为的实现效果。结果表明:参与合作社有助于提升农户的农产品质量控制行为;拥有自主农产品品牌的合作社更注重社员的农产品质量控制行为;生产流程培训作为中介渠道提高了合作社实施农产品质量控制行为,但对非品牌合作社质量控制行为的中介效应不显著。最后提出加强合作社生产质量管理、鼓励合作社实施农产品品牌化,注重合作社流程管理培训等政策建议。  相似文献   

硅对燕麦幼苗秆锈病抗病能力的作用     

李英浩  吕品  米俊珍  赵宝平  刘景辉  薛国兴 《中国农业大学学报》2022,27(8):68-75

为探究硅在燕麦抗秆锈病过程中的生理功能,以易感秆锈病的燕麦品种‘ 坝莜1号’为试验材料,采用盆栽方法,分别浇灌含不同浓度(0、0.5、1.0、1.5、2.0、2.5和3.0 mmol/L)K₂SiO₃的营养液,测定燕麦幼苗秆锈病抗性,叶片光合性能,抗氧化酶活性及渗透调节物质的含量等指标。结果表明:接种秆锈菌条件下,施用1.5 mmol/L K₂SiO₃可显著提高燕麦幼苗抗秆锈病能力及叶片的光合特性、抗氧化酶活性和渗透调节能力。与0 mmol/L(CK)相比,燕麦幼苗干和鲜重分别提高90.91%和74.71%,叶片叶绿素含量提高18.67%,净光合速率(P_n)、蒸腾速率(T_r)、气孔导度(G_s)和胞间CO₂浓度(C_i)分别提高51.47%、285.71%、285.71%和27.78%,最大荧光(F_m)、光化学效率(F_v/F_m)和光合性能指数(PI_ABS)分别提高19.62%、7.69%和5.84%,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性分别提高35.66%和28.88%,丙二醛(MDA)、可溶性蛋白及脯氨酸含量分别降低50.00%、27.45%和16.62%。总之,外源施用K₂SiO₃能提高燕麦抗秆锈病的能力,且以1.5 mmol/L为最佳浓度。  相似文献