全文获取类型
收费全文 | 94804篇 |
免费 | 4587篇 |
国内免费 | 10232篇 |
专业分类
林业 | 10570篇 |
农学 | 13680篇 |
基础科学 | 7925篇 |
14729篇 | |
综合类 | 30993篇 |
农作物 | 5254篇 |
水产渔业 | 3252篇 |
畜牧兽医 | 13144篇 |
园艺 | 4020篇 |
植物保护 | 6056篇 |
出版年
2024年 | 223篇 |
2023年 | 994篇 |
2022年 | 2543篇 |
2021年 | 3653篇 |
2020年 | 3200篇 |
2019年 | 3227篇 |
2018年 | 2248篇 |
2017年 | 3270篇 |
2016年 | 3057篇 |
2015年 | 4322篇 |
2014年 | 4193篇 |
2013年 | 5040篇 |
2012年 | 6234篇 |
2011年 | 6628篇 |
2010年 | 6551篇 |
2009年 | 6060篇 |
2008年 | 5577篇 |
2007年 | 5813篇 |
2006年 | 5399篇 |
2005年 | 4760篇 |
2004年 | 2330篇 |
2003年 | 2202篇 |
2002年 | 1913篇 |
2001年 | 1926篇 |
2000年 | 1996篇 |
1999年 | 2276篇 |
1998年 | 1994篇 |
1997年 | 1803篇 |
1996年 | 1643篇 |
1995年 | 1473篇 |
1994年 | 1256篇 |
1993年 | 1253篇 |
1992年 | 1034篇 |
1991年 | 886篇 |
1990年 | 733篇 |
1989年 | 590篇 |
1988年 | 467篇 |
1987年 | 263篇 |
1986年 | 153篇 |
1985年 | 72篇 |
1984年 | 53篇 |
1983年 | 48篇 |
1982年 | 54篇 |
1981年 | 53篇 |
1980年 | 40篇 |
1979年 | 14篇 |
1965年 | 16篇 |
1964年 | 12篇 |
1963年 | 12篇 |
1956年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
Immunogenic properties and mycoplasmal pneumonia of swine (MPS) lung lesions in Large White pigs selected for higher peripheral blood immune capacity
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Liushiqi Borjigin Tomoyuki Shimazu Yuki Katayama Meihua Li Takumi Satoh Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Kazuo Katoh Takafumi Uchida Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2016,87(5):638-645
Immunogenic properties and mycoplasmal pneumonia of swine (MPS) lung lesions were compared between the immunity‐selected Large White line and the non‐selected Large White line. The selected Large White line showed a higher level of pulmonary MPS lesions compared with the non‐selected Large White line. Subsequent to vaccination, the percentage of natural killer cells and T cells (CD3+CD4+CD8‐ and CD3+CD4?CD8+ T cells) were significantly increased in the non‐selected line but remained unchanged in the immunity‐selected Large White line. Secretion of Mycoplasma hyopneumoniae vaccine‐specific immunoblogulin G and phagocyte activity in peripheral blood were significantly higher in the immunity‐selected Large White line than in the non‐selected line. Expression of interleukin (IL)‐4 and IL‐6 messenger RNA in hilar lymph nodes was significantly lower in the immunity‐selected Large White line than in the non‐selected line. However, expression of IL‐10 in all immune tissues was significantly higher in the immunity‐selected Large White line. These results suggest that the selection for high immunity was not effective in increasing resistance to MPS lung lesions. 相似文献
982.
Xiao-ying He Li-bing Ma Xiao-ning He Wan-tong Si Yue-Mao Zheng 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):145-152
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%, respectively) and the transgenic group (80.8%, 26.5% and 53.4%); however, they were significantly higher than the bMGEs group (71.2%, 12.8% and 14.8%), (p < 0.05). We confirmed that hTERT-bMGEs could serve as effective target cells for improving development of somatic cell cloned cattle embryos. 相似文献
983.
Nanosize of zinc oxide and the effects on zinc digestibility,growth performances,immune response and serum parameters of weanling piglets
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ming‐Zhe Li Jie‐Ting Huang Yi‐Hao Tsai Syuan‐Yian Mao Chao‐Ming Fu Tu‐Fa Lien 《Animal Science Journal》2016,87(11):1379-1385
The aim of this study was to investigate the effect of dietary supplementation of nanosize zinc on zinc digestibility, growth performances, immune response and serum parameters of weanling piglets. Ninety‐six LYD weanling piglets were assigned to control, zinc oxide (ZnO), organic‐Zn (Zn‐methionine) and nanosize ZnO (nano‐Zn) groups with four replicates. The zinc was at the 120 mg/kg level in the treatment group's diet, while the control group's was 80 mg/kg Zn. The experiment results indicated that the nano‐Zn and organic‐Zn groups had significantly higher Zn digestibility compared to the ZnO and control groups. For the immune response traits, the IgG level and goat red blood cells (GRBC) antibody titer were nano‐Zn and organic‐Zn>ZnO>control; in the phytohemagglutinin (PHA) challenge test result, nano‐Zn>organic‐Zn>ZnO>control; in regard to the γ‐globulin level, nano‐Zn and organic‐Zn>ZnO and control, with significant difference between groups. In the serum parameters aspect, serum Zn concentration in nano‐Zn and organic‐Zn groups were higher than in the ZnO and control groups, serum growth hormone concentration was increased in the nano‐Zn group than in the other groups. In conclusion, nanosize zinc oxide for dietary supplementation can increase zinc digestibility, serum growth hormone levels and carbonic anhydrase activity and enhance the immune response of weanling piglets. 相似文献
984.
Effects of amino acids supplementation in low crude protein diets on growth performance,carcass traits and serum parameters in finishing gilts
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This study investigated the effects of amino acids (AA) supplementation in low crude protein (CP) diets on growth performance and carcass characteristics in finishing gilts. One hundred and eighty gilts (59.1 ± 5.1 kg) were randomly allotted to one of five diets which consisted of a high CP (15.6%) diet or four low CP (11.6%) diets for 50 days. The low CP diets were supplemented with lysine + threonine + methionine (LCM), LCM + tryptophan (LCT), LCT + valine (LCV) or LCV + isoleucine (LCI), respectively. Gilts were housed at six pigs per pen with six pens per treatment. At the end of the 50‐day experiment, 30 gilts (one pig per pen) with average body weight (BW) of 98 kg were killed to evaluate carcass traits. The pigs fed the diet supplemented with LCV obtained the highest average daily gain (ADG), which was higher than those of pigs fed the diet supplemented with LCM (P < 0.05). Dietary supplementation with tryptophan, valine and isoleucine in low CP diets increased ADG (linear and quadratic effect, P < 0.05), serum levels of valine (quadratic effect, P < 0.05) and isoleucine (linear and quadratic effect, P < 0.05) and immunoglobulin G (IgG) and IgA (linear and quadratic effect, P < 0.05) in finishing gilts. © 2016 Japanese Society of Animal Science 相似文献
985.
M.‐Y. Qian L. Wei D. Zhou Z.‐J. Zhang J.‐K. He Q.‐J. Zhang P. Zhu X.‐L. Xiao 《Journal of veterinary pharmacology and therapeutics》2016,39(1):84-89
Nanotechnology applications in medicine have seen a tremendous growth in the past decade and are being employed to enhance the stability and bioavailability of lipophilic substances, such as florfenicol. This study aimed to examine the pharmacokinetic properties of the formulated oil‐in‐water florfenicol‐loaded nanoemulsion (FF‐NE). FF‐NE and florfenicol control (Nuflor®) were administered to the pigs at a dose of 20 mg/kg. Nanoemulsion formulation of florfenicol was highly influenced in vivo plasma profile. The in vivo absorption study in pigs indicated that Cmax (14.54 μg/mL) was significantly higher in FF‐NE, 3.42 times higher than the marketed formulation. In comparison with the control group, the relative bioavailability of formulated nanoemulsion was up to 134.5%. Assessment of bioequivalence using log‐transformed data showed that the 90% confidence intervals (90% CI) of Cmax and AUC0–∞ were 2.48–4.60 and 1.21–1.72, respectively. 相似文献
986.
S. Li M. Johansson J. K. Vidanarachchi J. Pickova G. Zamaratskaia 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2016,66(4):199-205
The aim of this study was to develop a high-performance thin-layer chromatography densitometry method to simultaneously evaluate levels of different biogenic amines (BAs) in beef stored aerobically at 4°C. Steaks from M. longissimus thoracis et lumborum muscles were overwrapped with polyvinyl chloride film and stored for 0, 4, 7, and 10 days. For this purpose, the solvent system and pH for the derivatization of BAs with dansyl chloride were optimized. Moreover, this method was also validated for linearity, limits of detection and quantification, precision, and recovery. As found using this method in beef samples, spermine content decreased significantly after 7 days of storage, and tyramine and cadaverine were not detected until day 10. 相似文献
987.
Jianpeng Gao Mingxing Tian Yanqing Bao Peng Li Jiameng Liu Chan Ding Shaohui Wang Tao Li Shengqing Yu 《Veterinary research》2016,47(1)
Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism. 相似文献
988.
X.‐K. Li J.‐Z. Wang C.‐H. Zhang X. Li C.‐H. Tang 《Journal of animal physiology and animal nutrition》2016,100(2):271-278
To understand the background value of phosphorus in chickens, the quantitative distribution of different phosphorus forms, including total phosphorus (TP), free phosphate (FP) and phospholipid (PL), in viscera, blood and bones of broiler chickens was investigated. Results showed that phosphorus contents exhibited significant differences in different parts of chickens. TP content of breast and thigh meat was over 5.0 g/kg, in which most of the phosphorus was in the form of water‐soluble phosphates. TP content in viscera was higher than that in meat, and spleen was observed to contain the highest amount of phosphorus (10.0 g/kg). In all tested organs, FP and PL contents in liver were the highest, ranging between 1207–1989 and 81–369 mg/kg respectively. TP content in chicken bone was in the range of 52 716–136 643 mg/kg, and FP content in the bone was relatively lower than that in chicken meat. 相似文献
989.
Estimation of the standardized ileal digestible lysine requirement for primiparous pregnant sows
下载免费PDF全文
![点击此处可从《Journal of animal physiology and animal nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M. Shi C. X. Shi Y. K. Li D. F. Li F. L. Wang 《Journal of animal physiology and animal nutrition》2016,100(2):287-293
This experiment was conducted to determine the optimal standardized ileal digestible lysine (SID Lys) level in diets fed to primiparous sows during gestation. A total of 150 (Landrace × Large White) crossbred gilts (weighing 149.9 ± 3.1 kg) were fed gestation diets (12.55 MJ of ME/kg) containing SID Lys levels of 0.43, 0.52, 0.60, 0.70 or 0.80% respectively. Gilts were fed 2.0 kg/day from day 1 to 80 and 3.0 kg/day from day 80 to 110 of gestation respectively. Gilts were allocated to treatments based on their body weight on the day of breeding. Weight gain from day 80 to 110 increased with increasing dietary SID Lys levels (p = 0.044). Fitted broken‐line (p = 0.031) and quadratic plot (p = 0.047) analysis of body weight gain indicated that the optimal SID Lys level for primiparous sows was 0.70 and 0.69% respectively. During gestation, neither backfat thickness nor loin eye area was affected by dietary SID Lys level. Increasing dietary Lys had no effect on the litter size at birth or pigs born alive per litter. Litter weight at birth was not affected by dietary SID Lys level. The litter weight variation at birth quadratically decreased with increasing dietary SID Lys (p = 0.021) and was minimized at 0.70% dietary SID Lys. Gilts fed the 0.70% SID Lys diet had the highest dry matter (p = 0.031) and protein (p = 0.044) content in colostrum. On day 110 of gestation, gilts fed the 0.70% SID Lys diet tended to have the highest serum prolactin (p = 0.085) and serum insulin (p = 0.074) levels. The data demonstrate that the optimal dietary SID Lys was 0.70% for pregnant gilts, which is similar to the recommendation of 0.69% that was estimated by the NRC (2012). 相似文献
990.
Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep
下载免费PDF全文
![点击此处可从《Zeitschrift für Tierzüchtung und Züchtungsbiologie》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J. Wang H. Zhou J. Hu S. Li Y. Luo J.G.H. Hickford 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(3):219-226
Myostatin is a negative regulator of muscle growth and development in mammals, and variation in ovine myostatin gene (MSTN) has been demonstrated to be associated with variation in the muscularity of sheep. Polymerase chain reaction–single‐stranded conformational polymorphism (PCR–SSCP) was used to look for single nucleotide polymorphisms (SNPs) in a 304‐bp amplicon from the promoter region of ovine MSTN. Sequence analyses revealed two previously identified SNPs (c.?2449G/C and c. ?2379T/C) that resulted in three haplotypes (H1 (c.[?2449G; ?2379C]), H2 (c.[?2449C; ?2379C]) and H3 (c.[?2449G; ?2379T]). The effect of these SNPs on growth and carcass traits was investigated in 357 NZ Romney lambs. General linear mixed‐effect models revealed that sheep with the genotype c.?2449GC had a higher loin meat yield (p = 0.032) and proportion loin yield (p = 0.028), than those with the genotype c.?2449GG. The genotype c.?2379CC was associated with an increase in three weight traits: birthweight (p = 0.003), tailing weight (p = 0.009) and weaning weight (p = 0.028), when compared with the genotype c.?2379TC, but it was not found to have an association with growth rate. This suggests that c.?2379T/C has an effect that originates at, or before birth. Haplotype H3 was associated with a decrease in birthweight (p = 0.002), tailing weight (p = 0.003) and weaning weight (p = 0.011). Haplotype H2 was associated with increased loin yield (p = 0.012) and proportion loin yield (p = 0.002). The SNPs may have value as genetic markers for improved Romney breeding. 相似文献