猪圆环病毒与胸膜肺炎放线杆菌混合感染的诊治     

贾伟强  盛守志  胡林杰  孟野  翟海瑞  靳广杰  李旭阳  范增蕾  王桂芳 《现代畜牧兽医》2019,(5):37-40

2019年3月,大庆某规模化猪场个别猪出现发育不良、咳喘、消瘦、皮肤苍白和呼吸困难等症状,随后出现死亡。为明确发病原因,降低损失,对送检病死猪进行临床症状观察、剖检及实验室检测,并对病原菌进行药物敏感性试验。检测结果为致病性胸膜肺炎放线杆菌和猪圆环病毒混合感染所致,分离的胸膜肺炎放线杆菌对阿莫西林和头孢唑高度敏感。本研究为养殖场饲养猪的死亡病因分析和防治提供了参考。  相似文献   

Effect of chicken egg yolk immunoglobulins on serum biochemical profiles and intestinal bacterial populations in early‐weaned piglets     

Xian Tan  Jia Li  Yali Li  Jianzhong Li  Qingping Wang  Lin Fang  Xueqin Ding  Pengfei Huang  Huansheng Yang  Yulong Yin 《Journal of animal physiology and animal nutrition》2019,103(5):1503-1511

This study was conducted to test the hypothesis that dietary supplementation with anti‐E. coli, chicken egg yolk immunoglobulins (IgY), may affect early weaned piglet (EWP) intestinal functions and enteric micro‐organisms. One hundred and forty‐eight ([Landrace × Yorkshire] × Duroc) piglets, weaned at age day 21, were randomly assigned to receive one of three diets for 14 days. Treatment group one (control group) was fed the base diet. Treatment group two (antibiotics group) was fed the base diet which was supplemented with 100 ppm colistin sulphate and 15 ppm enramycin; treatment group three (IgY group) was fed the base diet which was supplemented with 500 mg/kg anti‐E. coli IgY. The study evaluated the effects on EWPs of IgY on growth, serum biochemical, inflammatory profiles and also digestion content intestinal bacterial populations. Results showed no significant difference in diarrhoea rates between IgY‐fed EWPs and antibiotic‐treated EWPs. Serum biochemical analysis showed that EWPs fed an IgY‐containing diet had both lower (p < 0.05) cholesterol and low‐density lipoprotein compared to antibiotic‐treated EWPs. Escherichia coli populations measured in IgY‐fed EWP ileal contents, compared to the control group, were significantly reduced (p < 0.05). Enterococcus, Lactobacillus, Clostridium and Bifidobacterium populations were unaffected by the IgY treatment. Larger (p < 0.05) Enterococcus populations and lower (p < 0.05) expression levels of heat‐stable enterotoxin b (STb) were observed in IgY‐fed EWP caecal digesta compared to the control group. Enteric Lactobacillus significantly decreased (p < 0.05) in EWPs fed antibiotics while it was unaffected by IgY treatment. Dietary supplementation with anti‐E. coli IgY has the potential to suppress enteric E. coli growth, but not Lactobacillus, Clostridium and Bifidobacterium. This promotes and maintains a healthy EWP intestinal environment. These findings suggest that IgY may be used as an alternative to antibiotics in EWP diets.  相似文献   

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C     

Na Wang  Kang Yang  Hai‐Tao Guo  Jing‐Ran Wang  Huan‐Huan Sun  Shun‐Wei Wang  Meng Sun  Liang‐Zheng Sun  Shun‐Li Yue  Jia‐Bo Zhou 《Reproduction in domestic animals》2019,54(8):1069-1077

Spermatozoa are highly specialized cells, and energy metabolism plays an important role in modulating sperm viability and function. Rosiglitazone is an antidiabetic drug in the thiazolidinedione class that regulates metabolic flexibility and glucose uptake in various cell types, but its effects on boar sperm metabolism are unknown. In this study, we investigated the potential effect of rosiglitazone against time‐dependent deterioration of boar spermatozoa during liquid preservation at 17°C. Freshly ejaculated semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations of rosiglitazone, and the motility, membrane and acrosome integrity of sperm were detected. Besides, we measured glucose uptake capacity, l ‐lactate production level, mitochondrial membrane potential, adenosine triphosphate (ATP) content and mitochondrial reactive oxygen species (mROS) production of sperm after boar semen had been incubated with or without rosiglitazone, iodoacetate (glycolysis inhibitor) and rotenone (electron transport chain inhibitor) for 5 days. The addition of rosiglitazone significantly enhanced sperm quality and had a strong protective effect on the sperm membrane and acrosome integrity during storage. BTS containing 50 μM rosiglitazone maintained the total motility of liquid‐preserved sperm above 60% for 7 days. Rosiglitazone improved sperm quality by regulating energy metabolism manner of preserved sperm, protected the sperm mitochondrial membrane potential, enhanced sperm ATP production and in the meanwhile reduced mROS through enhancing glycolysis but not oxidative phosphorylation. The data suggested the practical feasibility of using rosiglitazone for improving boar spermatozoa quality during semen preservation.  相似文献   

猪主要经济性状的基因组选择研究     

彭潇  尹立林  梅全顺  王海燕  刘小磊  朱猛进  李新云  付亮亮  赵书红 《畜牧兽医学报》2019,(2):439-445

旨在系统比较GBLUP、SSGBLUP、BayesA、BayesB、BayesC、BayesLASSO、BSLMM和BayesR等8种方法对猪重要经济性状基因组选择的准确性。本研究以本实验室收集的2 585头大白猪达100kg日龄、达100kg背膘厚和母猪乳头数3个性状为分析对象,结合猪50K基因芯片分型数据,以加性模型为基础,利用5倍交叉验证比较8种方法的基因组选择准确性。研究发现,基因组选择的准确性与不同性状估计遗传力呈正相关。交叉验证结果表明,预测准确性最高的性状为达100kg日龄,但不同方法在不同性状中表现并不完全相同,在达100kg日龄和达100kg背膘厚中SSGBLUP基因组预测准确性均为最高,而在母猪乳头数中BayesA的基因组预测准确性最高。综上表明,对小样本开展基因组预测时,中、高等遗传力性状可以选择SSGBLUP方法,低等遗传力性状可以选择BayesA方法。如何优化和选择一种广泛适用于所有性状的方法,可能是未来研究的方向。  相似文献   

MoHRD3基因参与调控稻瘟病菌的生长发育和致病力   总被引：1，自引：0，他引：1  

王如锋  文佳  赵桂媛  王敏  陈雪杭  Osakina Aron  王宗华  汤蔚 《植物病理学报》1955,50(1):68-79

 稻瘟病菌(Magnaporthe oryzae)作为主要的农业病原微生物,其引起的稻瘟病严重威胁着水稻等谷类作物的生产安全。内质网相关蛋白质降解途径(Endoplasmic reticulum-associated protein degradation, ERAD)是生物体应答内质网压力的主要方式之一,其在机体生长发育过程中具有重要作用。而HRD(HMG-CoA reductase degradation)复合物作为ERAD的关键组分,主要由Hrd1、Hrd3、以及凝集素Yos9等蛋白组成,负责内质网中错误折叠蛋白的识别、转运以及泛素化过程,最终由蛋白酶体降解,从而有效缓解内质网压力,保证细胞的正常生理活动。有研究表明,Hrd3属于单次跨膜蛋白,在内质网腔中与Hrd1、Yos9相结合,负责底物的识别并起着稳定Hrd1的作用。目前Hrd3在稻瘟病菌中的生物学功能尚不清楚。本研究通过基因敲除及互补试验获得了稻瘟病菌的ΔMohrd3突变体和ΔMohrd3-C回补菌株,并以野生型为对照,对突变体的生物学表型进行了分析。结果显示,ΔMohrd3突变体的生长速率、产孢量明显下降;对大麦和水稻的致病力显著减弱。进一步胁迫试验表明,MoHRD3的缺失导致稻瘟病菌对外界盐胁迫、渗透压胁迫的耐受性增强,对内质网胁迫耐受性减弱,而对细胞壁胁迫无明显变化。同时,MoHRD3基因的缺失激活了未折叠蛋白响应途径(Unfolded protein response, UPR)。上述结果表明,MoHRD3参与调控稻瘟病菌的营养生长、无性繁殖、致病及对不同环境胁迫的响应过程。  相似文献   

论新时期科技期刊青年编辑人才的创新素质     

栾嘉 《四川畜牧兽医学院学报》2008,(2):194-196

高素质的青年编辑人才是科技期刊发展的关键力量,其中创新素质起着举足轻重的作用。青年编辑在成长过程中,应该以正确理解科技期刊中的创新性和科技期刊编辑的刨新素质为前提,不断提高人文修养、注重知识的广度和深度、加强自身专业修养、加强主观能动性,从而达到提高创新素质的目的。  相似文献   

Analysis of petal anthocyanins to investigate coloration mechanism in herbaceous peony cultivars   总被引：1，自引：0，他引：1  

Ni Jia  Qing-Yan Shu  Liang-Sheng Wang  Hui Du  Yan-Jun Xu  Zheng-An Liu 《Scientia Horticulturae》2008

Petal coloration and anthocyanin compositions of 41 herbaceous peony cultivars were analyzed. Anthocyanins were identified by high-performance liquid chromatography–electrospray ionization-mass spectrometry (HPLC–ESI-MSⁿ) coupled with photodiode array detection (DAD). Peonidin-3,5-di-O-glucoside (Pn3G5G), pelargonidin-3,5-di-O-glucoside (Pg3G5G), cyanidin-3,5-di-O-glucoside (Cy3G5G), peonidin-3-O-glucoside (Pn3G), and cyanidin-3-O-glucoside (Cy3G) were the five major anthocyanins in herbaceous peony cultivars. Deep purple or reddish purple cultivars contained 4–5 anthocyanins, whereas pink cultivars only contained Cy3G5G and Pn3G5G, and their contents were much lower than those of purple cultivars. According to the chemical structures of three anthocyanidins in association with petal coloration, flowers were classified into three phenotypes: 1. “Pn, Cy, and Pg” (all purple flowers including two pink flowers); 2. “Pn, Cy” (pink flowers); 3 “Pn” (light pink and white flowers). The coloration mechanisms of cultivars with the pink and purple flowers were quite different. Correlations between lightness (L*) and chroma (C*), chromatic component a* and total anthocyanins (TA) value, a* and co-pigmentation index (CI) showed opposite tendencies, whereas L* and TA showed the same tendency in each group. High contents of Pn3G5G and Pg3G5G may responsible for the purple coloration of herbaceous peony cultivars.  相似文献   

SH系矮化中间砧对‘富士’苹果树体生长、产量和果实品质的影响   总被引：2，自引：0，他引：2  

李民吉  张强  李兴亮  周贝贝  杨雨璋  周佳  张军科  魏钦平 《园艺学报》2018,45(10):1999-2007

2009年春季定植不同类型SH系矮化中间砧(SH1、SH3、SH6、SH9和SH40)苹果苗(宫藤富士/SH系砧木/平邑甜茶),株行距为1.5 m×5.0 m,纺锤形整形修剪,常规管理。2016年调查树体生长、冠层光照分布和果实产量品质的差异,为苹果矮化砧木的筛选利用提供参考。研究结果表明,栽植第8年(2016年),各中间砧嫁接树体的砧穗干周比无显著差异;SH1和SH6中间砧嫁接的树体最矮,SH40最高且冠幅最大;树体干周直径由高到低依次为:SH40、SH3、SH9、SH6和SH1;各中间砧嫁接树体总枝量均超过140万条·hm~(-2);SH6嫁接树体短枝比例最高,树势中庸;SH3和SH9短枝比例最低。各中间砧嫁接树体冠层平均相对光照强度由高到低依次为SH3(72.88%)、SH6(70.85%)、SH9(65.51%)、SH1(62.23%)和SH40(61.82%),SH6嫁接树体相对光照强度小于30%和40%的区域最小,仅为2.08%和4.17%。比较各中间砧树体连续稳产4年的结果情况,SH6嫁接树体4年(2013—2016年)累计产量最高且稳产性最好,SH1产量较低,SH9稳产性较差。各中间砧嫁接树平均单果质量由高到低依次为SH40 SH6 SH9 SH3 SH1。不同中间砧嫁接树果实不同类型糖含量和比例存在显著差异;而总酸含量、不同类型酸的含量(除草酸外)和比例差异不显著;SH6嫁接树果实糖酸比显著高于SH1、SH3和SH9。综合树体的生长和结果能力,SH6为中间砧嫁接‘富士’表现较好。  相似文献   

Role of PI3K/Nrf2 signaling pathway in endotoxin-induced acute kidney injury in rabbits     

CHEN Mu-lin  YU Jian-bo  GONG Li-rong  SHI Jia 《园艺学报》2016,32(1):129-133

AIM: To evaluate the role of phosphatidylinositol 3-kinase/nuclear factor E2-related factor 2 (PI3K/Nrf2) signaling pathway in endotoxin-induced acute kidney injury in rabbits. METHODS: Healthy male New Zealand white rabbits were randomly divided into 5 groups: control group (group C), LPS group (group L), wortmannin+LPS group (group WL), wortmannin group (group W) and dimthyl sulfoxide (DMSO) group (group D). Wortmannin at dose of 0.6 mg/kg was injected via the auricular vein in groups W and WL, DMSO at concentration of 0.08 mL/kg was injected in group D, while normal saline (0.08 mL/kg) was injected in groups C and L. LPS at dose of 5 mg/kg was injected via the auricular vein in groups L and WL 30 min later, and equal volume of normal saline was injected in group C, D and W for control. The rabbits were sacrificed 6 h after LPS or normal saline administration. The kidneys were removed for microscopic examination and the determination of histological scores of kidney (HSK). The concentrations of blood urea nitrogen (BUN) and creatinine (Cr), urinary α1-microglobulin (α1-MG), MDA content, SOD activity, the mRNA expression of Nrf2 and HO-1, and the protein levels of total Akt, p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were also detected. RESULTS: Compared with groups C, D and W, the concentrations of BUN and Cr, urinary α1-MG concentration, MDA content and HSK were significantly increased, while SOD activity was significantly decreased (P<0.05). The mRNA expression of Nrf2 and HO-1, and the protein levels of p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were significantly increased in groups L and WL. No significant change among groups C, D and W was observed. Compared with group L, the concentrations of BUN and Cr, urinary α1-MG concentration, MDA content and HSK were significantly increased, while SOD activity, the mRNA expression of Nrf2 and HO-1, and the protein levels of p-Akt, total Nrf2, p-Nrf2, nuclear Nrf2 and HO-1 in the renal tissues were significantly decreased in group WL. CONCLUSION: Activation of PI3K/Nrf2 signaling pathway may be one of the regulatory mechanisms of the body adapting to the endotoxin-induced acute kidney injury in rabbits.  相似文献   

氮肥与行距对不同生育期虉草产量与品质的调控效应   总被引：1，自引：0，他引：1  

贾有余  骆秀梅  张永亮 《草业科学》2016,33(11):2312-2318

以通草1号虉草(Phalaris arundinacea cv.Tongcao No.1)为供试材料,采用行距(30、50、70cm)和施氮量(含氮量为0、46、92kg·hm~(-2))二因素裂区试验设计,研究了行距与施氮量对不同生育时期虉草营养成分含量的影响。结果表明,氮肥与行距对虉草产量和品质影响明显。随施氮量增加,各生育时期虉草产量、粗蛋白、粗灰分、钙和磷含量升高,而无氮浸出物含量下降。抽穗期虉草粗纤维和酸性洗涤纤维含量随施氮量增加而降低,而成熟期和秋季再生期随施氮量增加反而升高。随着行距增加各生育时期虉草产量下降。在9个处理组合中,以行距30cm、施氮量92kg·hm~(-2)组合抽穗期和成熟期草产量最高,以行距70cm、施氮量92kg·hm~(-2)组合饲草品质最好。抽穗期是虉草刈割利用的适宜时期,种植行距30cm、施氮92kg·hm~(-2)时可获得较高的饲草产量和粗蛋白质产量。  相似文献