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Ping Wan Lijun Ling Shuanghe Cao Xianping Wang Wenjun Zhang Hongqing Ling Lihuang Zhu Xiangqi Zhang 《Euphytica》2007,155(1-2):125-133
Special and degenerate primers are designed according to the conservative sequence of barley powdery mildew resistance genes
Mla1, Mla6, and Mla13. Two wheat Mla-like orthologs, TaMla-2 and TaMla-3 are cloned and sequenced from the cDNA of wheat resistant-powdery mildew line TAM104R by RT-PCR method. TaMla-2 and TaMla-3 encode distinct but highly related coiled-coil nucleotide-binding site leucine-rich repeat type (NBS-LRR) resistant disease
proteins and both reveal about 74 and 81% identity with amino acid sequence of Mla1, respectively. They are multiple copies in wheat genomes, one copy of them is mapped on wheat chromosome 1AL and two on 1BL using Chinese
Spring nulli-tetra-somic lines and ditelosomic lines of 1A, 1B and 1D in southern analysis. This result suggests that may
be the two Mla-like genes originated from the two diploid ancestral genomes, respectively. The expression pattern analysis of semi-quantitative
PCR shows the TaMla genes are mainly expressed in leaf and sheath, and expression level is enhanced in organs infected by Erysiphe
graminis, suggesting that TaMla-2 and TaMla-3 are powdery mildew resistance related-genes in wheat.
Electronic Supplementary Material Supplementary material is available for this article at 相似文献
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水稻再生能力和头季稻产量性状的QTL定位及其遗传效应分析 总被引：30，自引：2，他引：30
对籼稻窄叶青８号和粳稻京系１７杂交Ｆ１的花药进行离体培养，获得了１个含１３３个ＤＨ系的作图群体，通过构 建分子连锁图谱，对水稻再生能力，头季稻产量及其构成因子的ＱＴＬ进行区间作图。 相似文献
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A double haploid(DH) population derived from a cross between an indica variety Zaiyeqing 8(ZYQ 8) and a japonica variety Jingxi 17(JX 17), and their parents were completely submerged for 5 d at tillering stage to evaluate their submergence tolerance. 相似文献
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The functions of rice roots are absorption of water and nutrition, synthesis of amino acid and homone, and transportation of these substrates to overground parts. Rice root vitality is very important to the development of overground parts and yield. So far, many studies on the relationship between physiology condition and the root vitality, and their influence on the yield have been undertaken, and some QTLs for root growth characteristics, such as root length and root thick, have been identified. But the genetic base of the root vitality is still not clear. 相似文献
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