Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog     

Ayne Murata Hayashi  Júlia Maria Matera  Tatiana Soares da Silva  Ana Carolina Brand?o de Campos Fonseca Pinto  Sílvia Renata Gaido Cortopassi 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):95-98

A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of follow-up demonstrated that the dog was stable and had no recurrence of symptoms. Therefore, it was concluded that the combination of electroacupuncture and Chinese herbal medicine was responsible for motor rehabilitation.  相似文献   

Serum α-globulin fraction in horses is related to changes in the acute phase proteins     

Maria Victoria Carapeto DVM  Rafael Barrera DVM  PhD  Maria Cinta Mae DVM  PhD  Concepcin Zaragoza DVM  PhD 《Journal of Equine Veterinary Science》2006,26(3):120-127

Serum electrophoresis is a useful technique to diagnose some diseases characterized by quantitative and qualitative changes in the serum proteins, such as the acute phase proteins. An increased synthesis of acute phase proteins has been associated with acute infection and inflammation. We wanted to study whether gastrointestinal diseases and disorders of the locomotor system in horses produce enough inflammatory reaction so the acute phase proteins can be detected by electrophoresis in serum. Serum total proteins were calculated, and the serum electrophoresis from 63 horses was performed. The horses were classified in 3 groups: Group I (19 healthy horses), Group II (20 horses suffering from inflammatory disorders of the gastrointestinal tract), and Group III (24 horses with inflammatory disorders of the locomotor system). Results indicated an increase of the acute phase proteins only in patent inflammatory disorders that showed a rapid progression. A global increase in those proteins was observed only for α₁-globulins from Group II.  相似文献   

Poisoning of horses by bamboo, Bambusa vulgaris     

Jos Diomedes Barbosa  Carlos Magno C. de Oliveira  Marcos Dutra Duarte  Gabriela Riet-Correa  Paulo Vargas Peixoto  Carlos Hubinger Tokarnia 《Journal of Equine Veterinary Science》2006,26(9):393-398

The clinical and pathological aspects of a neurological disease observed in 16 horses in Pará, Amazonia, Brazil, are presented. The symptoms were mainly motor incoordination, paresis of the tongue, somnolence, difficulties in apprehension, chewing and swallowing of food, as well as instability and standing with abducted members. The clinical course was subacute or chronic and in most cases was not fatal. Postmortem examination performed in one already very sick, euthanized animal, did not show significant macroscopic lesions; histopathological examination revealed slight edema and degenerative alterations of a few axons, mainly in the medulla oblongata. In all pastures where horses were affected, plenty of bamboo had been eaten, probably because of scarcity of pasture. By feeding large amounts of fresh bamboo leaves of this region, in different growing stages, to three horses (horse 1, 47 g/kg/d for 30 days; horse 2, 10 g/kg/d for 60 days; horse 3, 18 g/kg on the first day, and 31 g/kg/d for 6 more days)—the animals ate the leaves unassisted—it was possible to reproduce nervous symptoms essentially identical to those observed in the natural disease 24 to 72 hours after the first feeding of the plant. In spite of continuous administration of the plant, intensity of the clinical signs did not increase. Based on field observations and comparison of the clinical and pathological pictures seen in the natural and experimental disease, the described illness can be concluded to be caused by the ingestion of large amounts of the leaves of Bambusa vulgaris f. vulgaris.  相似文献   

A comparison of nitrogen use efficiency definitions in Citrus rootstocks     

Agostino Sorgonà  Maria Rosa AbenavoliPietro Giorgio Gringeri  Giovanni Cacco 《Scientia Horticulturae》2006

Nitrogen use efficiency (NUE) definitions, commonly used in literature, are evaluated in response to nitrate availability in four citrus rootstocks, Rough Lemon (Citrus jambhiri Lush) (RL), Sweet Orange (Citrus sinensis (L.) Osbeck) (SwO), Cleopatra Mandarin (Citrus reshni Hort ex Tan.) (CM) and Sour Orange (Citrus aurantium L.) (SO). The application of diverse definitions determine different characterizations in N-efficiency among rootstocks. Nitrogen utilization efficiency (NUtE) and nitrogen efficiency ratio (NER) determine equal level of nitrogen efficiency among all rootstocks. Total nitrogen accumulation (TNA), nitrogen uptake efficiency (NUpE), shoot dry weight (SDW) and total leaf area (TLA) response curves produce the same NUE characterization: SO and SwO were nitrate use efficient and inefficient rootstocks, respectively, while the RL and CM exhibit superior and inferior genetic potential, respectively.  相似文献   

Uncoupling and inhibition properties of 3,4-seco-friedelan-3-oic acid isolated from Maytenus imbricata     

Silvia Ribeiro de Souza e Silva  Luiz Claúdio de Almeida Barbosa  Beatriz King-Diaz  Blas Lotina-Hennsen 《Pesticide biochemistry and physiology》2007,87(2):109-114

3,4-Seco-friedelan-3-oic acid was isolated from Maytenus imbricata (Celastraceae). At low concentrations it inhibited non-cyclic electron transport and ATP synthesis in spinach chloroplasts, i.e., it behaved as a Hill reaction inhibitor, and at high concentrations it acts as an uncoupler by enhancing uncoupled electron transport and Mg²⁺-ATPase activity. 3,4-Seco-friedelan-3-oic acid did not inhibit PSII electron transport from DPC to DCPIP_ox and photosystem I activity, but it enhanced from TMQH₂ to MV, corroborating its action as uncoupler. It inhibits electron flow through PSII from water to sodium silicomolybdate. The whole results indicate that the 3,4-seco-friedelan-3-oic acid target is at the OEC complex enzyme, the donor side of PSII. The fluorescence decay data shows the formation of the K-band, which match this result, acting as inhibitor at the donor side of PSII and it as an uncoupler.  相似文献   

芝麻菜品种对尖镰孢菌枯萎病的抗性评价          下载免费PDF全文

陈国康  Gilardi Giovann  Gullino Maria Lodovica 《植物保护学报》2007,34(1):37-45

尖镰孢菌枯萎病是意大利西北部芝麻菜栽培品种(Eruca vesicaria)和野生品种(Diplotaxis spp．)上的重要病害之一,常引起严重的损失。为评价芝麻菜品种对尖镰孢菌枯萎病的抗性,2004年6～11月,选用了44个栽培及野生品种资源在玻璃温室进行抗性试验,使用浓度为1×10~6cfu/ mL的孢子悬浮液蘸根法人工接种30天苗龄的芝麻菜幼苗,以分离自芝麻菜两个不同野生品种的菌株Fus．Ruc．9A/02和Fus．Ruc．13/03,以及分离自栽培和野生品种的32个菌株混合(MIX),分别作为抗病试验接种体,每次接种试验设A、B、C三个重复。整个试验重复进行2次。调查病害引起的死苗率(%)和病情指数(DI),作为评价芝麻菜品种抗性差异的两项指标。研究结果显示,这两项指标间的相关性极显著(P＜0．01),即表明可采用其中一项指标评价芝麻菜品种对尖镰孢菌枯萎病的抗性。供试的44个栽培及野生芝麻菜品种中,多数品种抗尖镰孢茵茵株Fus．Ruc．13/ 03,而对菌株Fus．Ruc．9A/02及菌株混合MIX表现为易感性,而且这些易感品种在菌株Fus．Ruc．9A/02及菌株混合MIX两试验中的抗性表现较为一致,Fus．Ruc．9A/02和MIX的毒性也更强于Fus．Ruc．13/03菌株。芝麻菜品种12/03和品种20/03高抗菌株混合MIX,品种6/03和品种7/04高抗菌株Fus．Ruc．9A/02,而品种9/02、2/03、5/03、6/03、7/03、9/03、11/03、12/03、20/03、21/03、24/03、3/04、6/04、7/04和11/04高抗菌株Fus．Ruc．13/03。  相似文献   

Effects of cropping history and origin of seed potatoes on population structure of Phytophthora infestans     

Heidi Bouws  Maria R. Finckh 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(4):313-327

The effects of origin of seed potatoes and the cropping history on the phenotypic structure of Phytophthora infestans populations was studied in northern Hessia, central Germany, from 2000 to 2002. Populations originating from fields with a history of potato cropping with only short or no rotation (old fields) were compared with populations from new fields, i.e., where no potatoes had been grown for at least 30 years and seed potatoes were either imported from breeders or produced on-farm (certified). The main goal was to determine the importance of seed potato infection in the establishment of new P. infestans populations. Isolates were characterized for mating type, virulences and rep- (repetitive extragenic palindromic) PCR fingerprints. Among a total of 639 isolates sampled from 31 sites, mating types A1 and A2 co-existed in all three years in 60–92% of the sites. Over all three years, 53 pathotypes were detected in a subsample of 272 isolates. Isolates originating from the new fields had significantly higher frequencies of the virulences v1, v2, v3, v6 and v7, indicating general effects of seed introduction into a new region. Thirty-six fingerprints were detected in a subsample of 281 isolates of which 22 were unique while four occurred in all three years and in many sites. Pathogen populations from potato fields that were grown from seed tubers of geographically different origin differed significantly based on χ ² tests. While the Nei genetic distances were less than .1 among the local populations, distances to the US lineages US-1, US-6, US-7 and US-8 ranged from .22 to .47; however, the bootstrap values were not significant. Populations from old fields were more diverse and 14 of the 22 rep-PCR types occurred there among 132 isolates tested in comparison to six in the new fields (n = 140 isolates) and two among six isolates from volunteers. The results also suggest that both sexual and asexual reproduction play a role.  相似文献   

Ineffectiveness of pruning to control citrus huanglongbing caused by <Emphasis Type="Italic">Candidatus</Emphasis> Liberibacter americanus     

Silvio Aparecido Lopes  Guilherme Fernando Frare  Pedro Takao Yamamoto  Antonio Juliano Ayres  José Carlos Barbosa 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):463-468

The huanglongbing (HLB) disease of citrus trees, caused by Candidatus Liberibacter asiaticus and Ca. Liberibacter americanus, was first reported in Brazil in March, 2004. The presence of the disease has caused serious concerns among growers. Pruning experiments were conducted to determine if removal of symptomatic branches or the entire canopy (decapitation) would eliminate infected tissues and save HLB-affected trees. Pruning was done in five blocks on a total of 592 3- to 16 year-old ‘Valência’, ‘Hamlin’ or ‘Pêra’ sweet orange trees showing no symptoms or with two levels of symptom severity. Ten decapitated trees per block were caged and all trees were treated with insecticides to control the psyllid vector, Diaphorina citri. Mottled leaves reappeared on most symptomatic (69.2%) as well on some asymptomatic (7.6%) pruned trees, regardless of age, variety, and pruning procedure. Presence of the pathogen (Ca. Liberibacter americanus) in all symptomatic trees was confirmed by PCR. In general, the greater the symptom severity before pruning the lower the percentage of trees that remained asymptomatic after pruning.  相似文献   

Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop     

F. A. Zuckermann  M. D. Pescovitz  B. Aasted  J. Dominguez  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. Boyd  J. Walker  R. Lee  W. C. Davis  I. R. Barbosa  A. Saalmü  ller 《Veterinary immunology and immunopathology》1998,60(3-4):291-303

Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, #090; UCP1H12-2, #139; and PG164A, #051) bind exclusively to CD8⁺ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 33 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8⁺ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8^low and CD8^high cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8^low and CD8^high lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4⁻/CD8^high lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, #094; and SwNL554.1, #009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8⁺ lymphocyte subsets in health and disease.  相似文献   

Discrimination of ‘San Marzano’ accessions: A comparison of minisatellite, CAPS and SSR markers in relation to morphological traits     

Martina Caramante  Rosa Rao  Luigi Maria Monti  Giandomenico Corrado   《Scientia Horticulturae》2009,120(4):560-564

‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain.  相似文献