Proliferation capacity, neuronal differentiation potency and microstructures after the differentiation of canine bone marrow stromal cells into neurons     

Edamura K  Kuriyama K  Kato K  Nakano R  Teshima K  Asano K  Sato T  Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(7):923-927

We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, the microstructures of neuron-like cells after neuronal differentiation were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, and 3.8 ± 2.1 × 10(6) BMSCs were collected in one passage. Approximately 65% of canine BMSCs changed to neuron-like morphology after neuronal differentiation, and nearly all neuron-like cells stained positive against neuron-specific enolase. In addition, microstructures such as the cellular organelles, filaments and growth cones of these cells bore a close resemblance to those of the original mature neurons. These results suggested that canine BMSCs might be capable of differentiating into neurons.  相似文献   

Mitigation of pyrexia by a Th-1-biased IgG subclass response after infection with equine herpesvirus type 1 in horses pre-immunized with inactivated vaccine     

Bannai H  Tsujimura K  Kondo T  Nemoto M  Yamanaka T  Sugiura T  Kato T  Maeda K  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):791-795

The immunoglobulin G (IgG) subclass response was investigated in horses with or without pyrexia after natural infection with equine herpesvirus type 1 (EHV-1) in the field. All horses were kept at the training centers of the Japan Racing Association and were immunized with an inactivated EHV-1 vaccine before EHV-1 infection. An IgG subclass response dominated by IgGa and IgGb was induced in horses without pyrexia after EHV-1 infection. In contrast, horses that developed pyrexia showed increased IgGc and IgG (T) subclass production in addition to IgGa and IgGb. Although inactivated EHV-1 vaccines are considered to induce a mainly Th-2-biased response, these results indicated that the responses in horses inoculated with inactivated EHV-1 vaccine were not uniform, and that horses with a Th-1-biased response were likely to be protected from pyrexia.  相似文献   

Ventrocaudal cystorrhaphy without urethral sphincterotomy in two broodmares     

T. Higuchi  S. Inoue  M. Satoh  T. Kato  Y. Yuasa 《Equine Veterinary Education》2012,24(10):513-516

Two broodmares were diagnosed with rupture of the urinary bladder. One mare revealed abnormalities post partum and the other associated with ante partum uterine torsion. The clinical symptoms included mild abdominal pain, anorexia, decreased urinary volume and increased peritoneal fluid. In one mare, based on the creatinine level of the peritoneal fluid and serum biochemical abnormalities, uroperitoneum was diagnosed. In the other mare, the bladder rupture was found during the celiotomy for surgical repair of uterine torsion that was diagnosed upon rectal examination. Surgery was performed without a urethral sphincterotomy. The vaginal floor was incised in a standing position and the bladder was diverted into the vagina in order to suture the tears located in the ventrocaudal aspect of the bladder. Both mares survived after treatment for uraemia. Bladder rupture, although uncommon, may affect peripartum mares. Approach to the ruptured bladder without urethral sphincterotomy in a standing position should be considered as a choice for surgical repair.  相似文献   

Distribution of photoperiod-insensitive allele Ppd-A1a and its effect on heading time in Japanese wheat cultivars     

Masako Seki  Makiko Chono  Tsutomu Nishimura  Mikako Sato  Yasuhiro Yoshimura  Hitoshi Matsunaka  Masaya Fujita  Shunsuke Oda  Katashi Kubo  Chikako Kiribuchi-Otobe  Hisayo Kojima  Hidetaka Nishida  Kenji Kato 《Breeding Science》2013,63(3):309-316

The Ppd-A1 genotype of 240 Japanese wheat cultivars and 40 foreign cultivars was determined using a PCR-based method. Among Japanese cultivars, only 12 cultivars, all of which were Hokkaido winter wheat, carried the Ppd-A1a allele, while this allele was not found in Hokkaido spring wheat cultivars or Tohoku-Kyushu cultivars. Cultivars with a photoperiod-insensitive allele headed 6.9–9.8 days earlier in Kanto and 2.5 days earlier in Hokkaido than photoperiod-sensitive cultivars. The lower effect of photoperiod-insensitive alleles observed in Hokkaido could be due to the longer day-length at the spike formation stage compared with that in Kanto. Pedigree analysis showed that ‘Purple Straw’ and ‘Tohoku 118’ were donors of Ppd-A1a and Ppd-D1a in Hokkaido wheat cultivars, respectively. Wheat cultivars recently developed in Hokkaido carry photoperiod-insensitive alleles at a high frequency. For efficient utilization of Ppd-1 alleles in the Hokkaido wheat-breeding program, the effect of Ppd-1 on growth pattern and grain yield should be investigated. Ppd-A1a may be useful as a unique gene source for fine tuning the heading time in the Tohoku-Kyushu region since the effect of Ppd-A1a on photoperiod insensitivity appears to differ from the effect of Ppd-B1a and Ppd-D1a.  相似文献   

Genomic gene encoding manganese peroxidase from a white-rot fungus Phanerochaete crassa WD1694     

Mariko Takano  Muneysohi Yamaguchi  Hiroaki Sano  Masaya Nakamura  Hajime Shibuya  Yasumasa Miyazaki 《Journal of Wood Science》2013,59(2):141-148

The gene encoding manganese peroxidase of a white-rot fungus Phanerochaete crassa WD1694 was cloned and sequenced. Four genomic clones were sequenced in which 3 clones were existed as alleles. The analysis of intron–exon structures divided the 4 clones into three subfamilies that corresponded to mnp2 and mnp3 of Phanerochaete chrysosporium, and a new subfamily possessing only five introns. The purified P. crassa WD1694 MnP consisted of 4 isozymes with same molecular weight, same N-terminal sequence, and different pI. N-terminal sequence of deduced protein of P. crassa mnpB3 gene was identical to those of 4 MnP isozymes; however, the other 3 mnp genes had different N-terminal sequence. The molecular weight of encoded mature protein of mnpB3 gene and purified MnP had a gap that could be difference between MnP proteins encoded by single gene. The results suggested that 4 MnP isozymes of P. crassa WD1694 arose from single gene.  相似文献   

Correlation of seedling size and branch number with disease resistance of Pinus thunbergii seedlings to Bursaphelenchus xylophilus     

T. Hakamata  K. Kato  S. Yamamoto 《Forest Pathology》2013,43(3):238-244

Japanese black pine (Pinus thunbergii) seedlings resistant to pine wood nematode (PWN; Bursaphelenchus xylophilus) are routinely selected in Japanese field inoculation trials. Correlations between morphological factors (such as height, stem diameter at ground level and number of branches on seedlings) and disease resistance were examined to improve the production efficiency of 1‐year‐old black pine seedlings for inoculation. Family relatedness and environmental conditions strongly affected seedling resistance; accordingly, logistic regression analysis was used to separate effects of these two variables. Height and stem diameter at ground level significantly correlated with disease resistance in seedlings inoculated with PWN. Because (a) interactions between stem diameter at ground level and environmental condition were significant and (b) height did not interact with any other factor, it was concluded that height of 1‐year‐old Japanese black pine seedlings independently correlated with PWN resistance. Thus, field inoculation tests should use tall seedlings to achieve enhanced survival rates.  相似文献   

GIS analysis of conjunctive water resource use in Nganjuk district, east Java, Indonesia     

Liyantono  Tasuku Kato  Hisao Kuroda  Koshi Yoshida 《Paddy and Water Environment》2013,11(1-4):193-205

Water shortages during the dry season threaten sustainable agricultural production in Nganjuk District, East Java, Indonesia. To mitigate this problem, farmers adopted conjunctive use of surface water and groundwater, but the sustainability of this practice has not been investigated. This study temporally and spatially assessed water allocation in Nganjuk District when conjunctive irrigation was used. In particular, the land cover, water balance, and irrigation well density (IWD) were analyzed using time series GIS and remote sensing data to obtain their temporal and spatial distributions. First, the land cover was analyzed to determine cropping intensity, and the water balance was analyzed temporally and spatially. IWD was introduced to facilitate the water balance analysis. Second, the land cover, water balance, and IWD results were overlaid. Third, the effectiveness of the IWD method, the magnitude of water shortages, and the sustainability of groundwater resources were considered. Temporal and spatial water shortages in irrigation blocks were observed during the dry season. The change of storage showed a surplus during the wet and early dry seasons and a shortage during the late dry season. The annual water balances indicated that the southern part had a surplus, and the northern part experienced water shortages, especially downstream of the Widas River. Conjunctive use during the late dry season was predominant and concentrated in the southern part (83% of southern blocks). IWD was appropriate for examining groundwater use trends and was effective for expressing average withdrawal data (R ² = 0.87).  相似文献   

A simple,low‐cost technique for in situ measurement of leaf P concentration in field‐grown rice     

Koji Yamane  Ranee C. Mabesa-Telosa  Ryosuke Tajima  Nino P. M. C. Banayo  Yoichiro Kato 《植物养料与土壤学杂志》2019,182(1):28-30

Simple tools for diagnosing crop nutritional status are in great demand by agricultural extension staff, particularly in low‐fertility environments. We developed the first practical method for in situ diagnosis of phosphorus (P) nutrition in field‐grown cereal crops by using a handheld colorimeter. The concentration of P in extracts from fresh leaves of lowland rice grown under various P availabilities was closely correlated with that of oven‐dried leaves measured with the standard molybdenum‐blue method in the laboratory, over a range of values from 0.319 to 1.420 mg P g ^?1 DW (r = 0.885^**, n = 14). It takes only 3–5 min to estimate the P concentration of plant samples using the new technique. Although not as accurate as the laboratory method, the new method can easily detect P deficiency of rice in the field (and possibly other cereal crops) without requiring costly, off‐site equipment. Thus, extension staff and agronomists can easily adopt this technique.  相似文献   

Development of a screening method for genetically modified soybean by plasmid-based quantitative competitive polymerase chain reaction     

Shimizu E  Kato H  Nakagawa Y  Kodama T  Futo S  Minegishi Y  Watanabe T  Akiyama H  Teshima R  Furui S  Hino A  Kitta K 《Journal of agricultural and food chemistry》2008,56(14):5521-5527

A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents.  相似文献   

Turbid water induces refuge behaviour of a commercially important ayu: A field experiment for interstream movement using multiple artificial streams          下载免费PDF全文

Terutaka Mori  Yasumitsu Kato  Tetsuya Takagi  Yukio Onoda  Yuichi Kayaba 《Ecology of Freshwater Fish》2018,27(4):1015-1022

Riverine systems are inherently dendritic in nature, and turbid water may not necessarily flow equally throughout an entire catchment. Tributaries have the potential to serve as refugia from temporarily turbid water in the main stem. As ayu (Plecoglossus altivelis) are one of the important species for the inland fisheries industry and recreational fishing, their behaviour in a branched river network is a primary concern. We released ayu with radio‐transmitting tags into two experimental streams that joined at a downstream pool. Turbid water was released into one of the streams, and we identified whether avoidance movement of ayu between the two experimental streams was triggered by flowing turbid water. The suspended solid concentration elevated rapidly after adding the turbid water and was maintained at more than 400 mg/L for 3 hr. Avoidance movement of ayu was triggered when the suspended solid concentration exceeded approximately 200 mg/L. Then, most ayu moved from the turbid stream to the nonturbid one, leading to a difference in ayu density between the two streams. Therefore, we demonstrated that turbid water induced interstream movement of a commercially important ayu as refuge behaviour, suggesting that river connectivity is important for the persistence of the ayu.  相似文献