Comparison of the anthocyanin composition during ripening of Syrah grapes grown using organic or conventional agricultural practices     

Vian MA  Tomao V  Coulomb PO  Lacombe JM  Dangles O 《Journal of agricultural and food chemistry》2006,54(15):5230-5235

The anthocyanin composition of Syrah grapes harvested at different stages of ripening and produced using organic or conventional agriculture was studied. Samples of grapes were collected from veraison to full maturity in each plot, and the content in nine anthocyanins was determined by high-performance liquid chromatography with diode array detection. The total content in anthocyanins during ripening of the conventionally grown grapes was significantly higher compared to that found in the organic production. The accumulation of anthocyanins reached a maximum 28 days after veraison (in agreement with high temperature) and then decreased until harvest. In all samples, grapes from the conventional agriculture presented higher proportions of delphinidin, petunidin, malvidin, and acylated malvidin glucosides compared to grapes from organic agriculture. In contrast with other comparative studies of organically and conventionally grown plants, the results demonstrated a higher content in anthocyanins in conventionally grown grapes.  相似文献   

A century of Alzheimer's disease     

Goedert M  Spillantini MG 《Science (New York, N.Y.)》2006,314(5800):777-781

One hundred years ago a small group of psychiatrists described the abnormal protein deposits in the brain that define the most common neurodegenerative diseases. Over the past 25 years, it has become clear that the proteins forming the deposits are central to the disease process. Amyloid-beta and tau make up the plaques and tangles of Alzheimer's disease, where these normally soluble proteins assemble into amyloid-like filaments. Tau inclusions are also found in a number of related disorders. Genetic studies have shown that dysfunction of amyloid-beta or tau is sufficient to cause dementia. The ongoing molecular dissection of the neurodegenerative pathways is expected to lead to a true understanding of disease pathogenesis.  相似文献   

Tectonic uplift and Eastern Africa aridification   总被引：2，自引：0，他引：2  

Sepulchre P  Ramstein G  Fluteau F  Schuster M  Tiercelin JJ  Brunet M 《Science (New York, N.Y.)》2006,313(5792):1419-1423

The history of Eastern African hominids has been linked to a progressive increase of open grassland during the past 8 million years. This trend was explained by global climatic processes, which do not account for the massive uplift of eastern African topography that occurred during this period. Atmosphere and biosphere simulations quantify the role played by these tectonic events. The reduced topographic barrier before 8 million years ago permitted a zonal circulation with associated moisture transport and strong precipitation. Our results suggest that the uplift itself led to a drastic reorganization of atmospheric circulation, engendering the strong aridification and paleoenvironmental changes suggested by the data.  相似文献   

Effect of fetal bovine serum and heat-inactivated fetal bovine serum on microbial cell wall-induced expression of procoagulant activity by equine and canine mononuclear cells in vitro     

Okano S  Hurley DJ  Vandenplas ML  Moore JN 《American journal of veterinary research》2006,67(6):1020-1024

OBJECTIVE: To determine the effect of fetal bovine serum (FBS) and heat-inactivated FBS (HI-FBS) on lipopolysaccharide (LPS)- and zymosan-induced procoagulant activity of equine and canine mononuclear cells. SAMPLE POPULATION: Mononuclear cells from 18 horses and 3 dogs. PROCEDURES: Cells were incubated with various concentrations of FBS, HI-FBS, LPS, zymosan, polymyxin B, and anti-LPS-binding protein monoclonal antibody or combinations of these constituents. A 1 stage recalcification assay was used to determine procoagulant activity. RESULTS: Addition of FBS to media significantly increased procoagulant activity; equine and canine cells were stimulated by 1% and 10% FBS, respectively. Coincubation of cells with FBS and polymyxin B did not reduce this effect, suggesting that the response was not attributable to LPS contamination. Addition of HI-FBS to media did not stimulate procoagulant activity of equine or canine cells, and the sensitivity of the equine cells to LPS was significantly increased by HI-FBS. This increased LPS sensitivity was reduced 40% with monoclonal antibody directed against human recombinant LPS-binding protein. Increasing concentrations of HIFBS significantly increased LPS- and zymosan-induced procoagulant activity of canine cells. CONCLUSION AND CLINICAL RELEVANCE: Procoagulant activity production in equine and canine mononuclear cells was significantly increased by addition of FBS, whereas heat inactivation of FBS eliminated this effect. Heat inactivation did not eliminate the function of serum proteins involved in enhancement of LPS and zymosan-induced procoagulant activity. Results suggest that HI-FBS can be used as a source of serum proteins that increase the sensitivity of mononuclear cells to bacterial and yeast cell wall components.  相似文献   

Airborne fungal succession in a rice field of Cuba     

Michel Almaguer  Teresa Irene Rojas  Francisco Javier Rodríguez-Rajo  María Jesús Aira 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(2):473-482

This study characterised the airborne mycobiota of a rice plantation in Bauta (Havana, Cuba), with a view to improving crop quality. Culturable airborne fungi were sampled between March 2007 and February 2008 using a volumetric method (SAS Super 100) with potato dextrose agar as culture medium. The total fungal count was 51,425 cfu/m³. The 39 filamentous genera identified included several major rice pathogens (Bipolaris, Curvularia, Alternaria, Pyricularia, Cercospora and Fusarium) as well as potentially mycotoxigenic species of Fusarium, Penicillium and Aspergillus. Analysis of the influence of weather-related factors on spore counts revealed that total counts and Penicillium counts correlated positively with daily relative humidity, mean temperature and dew point.  相似文献   

Topography of the northern hemisphere of Mercury from MESSENGER laser altimetry     

Zuber MT  Smith DE  Phillips RJ  Solomon SC  Neumann GA  Hauck SA  Peale SJ  Barnouin OS  Head JW  Johnson CL  Lemoine FG  Mazarico E  Sun X  Torrence MH  Freed AM  Klimczak C  Margot JL  Oberst J  Perry ME  McNutt RL  Balcerski JA  Michel N  Talpe MJ  Yang D 《Science (New York, N.Y.)》2012,336(6078):217-220

Laser altimetry by the MESSENGER spacecraft has yielded a topographic model of the northern hemisphere of Mercury. The dynamic range of elevations is considerably smaller than those of Mars or the Moon. The most prominent feature is an extensive lowland at high northern latitudes that hosts the volcanic northern plains. Within this lowland is a broad topographic rise that experienced uplift after plains emplacement. The interior of the 1500-km-diameter Caloris impact basin has been modified so that part of the basin floor now stands higher than the rim. The elevated portion of the floor of Caloris appears to be part of a quasi-linear rise that extends for approximately half the planetary circumference at mid-latitudes. Collectively, these features imply that long-wavelength changes to Mercury's topography occurred after the earliest phases of the planet's geological history.  相似文献   

Smallholder Agroforestry in Rwanda: A SWOT-AHP Analysis     

G. Andrew Stainback  Michel Masozera  Athanase Mukuralinda  Puneet Dwivedi 《Small-Scale Forestry》2012,11(3):285-300

The perception of Rwandan government officials, NGOs, and extension specialists about smallholder agroforestry adoption as a strategy for smallholder farmers in Rwanda was investigated using a strengths, weaknesses, opportunities, and threats analysis framework combined with the analytical hierarchy process. Results indicate that smallholder agroforestry is viewed positively as a suitable strategy for Rwandan smallholder farmers. The most important positive features were the potential for increased agricultural output from agroforestry and a favorable policy environment in Rwanda supporting sustainable agriculture. Results also indicate that there needs to be better coordination of various efforts to promote agroforestry and stronger extension services for smallholder farmers. Carbon offset markets and other environmental service markets were seen as a potential opportunity for smallholder agroforestry. However, the results also indicate that there is substantial uncertainty and skepticism concerning how such markets would benefit smallholder farmers who adopted agroforestry.  相似文献   

Rapid MALDI-TOF mass spectrometry identification of Leptospira organisms: A reply     

Djelouadji Z  Roux V  Raoult D  Kodjo A  Drancourt M 《Veterinary microbiology》2012,159(3-4):544

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Allelic polymorphism in the second exon of Ovar-DRB1 in fat-tailed sheep     

Nikbakht G  Rezaii H  Stear MJ  Talebi MA  Mahmoudzadeh H 《Veterinary journal (London, England : 1997)》2012,192(3):547-549

Ovar-DRB1 is one of the most important response genes in the major histocompatibility complex (MHC) class II region of sheep. Gene polymorphism in the second exon of Ovar-DRB1 in three different Iranian fat-tailed sheep breeds (Lori-Bakhtiari, Shaul and Zandi) was analyzed by either restriction fragment length polymorphisms (RFLP) or direct sequencing. A total of 92 Lori-Bakhtiari, 40 Shaul, and 47 Zandi sheep were examined. PCR-RFLP identified 17 genotypes in Lori-Bakhtiari sheep, 12 in Shaul sheep and 11 in Zandi sheep. Collectively, 24 different genotypes could be found for Iranian fat-tailed sheep. Using direct sequencing, seven new sequences in exon 2 of the Ovar-DRB1 gene were identified. Generalized linear modeling with a multinomial error structure showed that the sheep populations had significantly different allele frequencies.  相似文献   

From problem to success: feline weight loss programs that work     

Michel K  Scherk M 《Journal of Feline Medicine and Surgery》2012,14(5):327-336

PRACTICAL RELEVANCE: Obesity is the most common unhealthy nutritional condition that is recognized in cats. Documented associated health risks include diabetes mellitus, lameness, non-allergic skin disease, feline lower urinary tract disease and idiopathic hepatic lipidosis. CLINICAL CHALLENGES: Simply recommending a diet designed for weight loss fails, in most cases, to result in successful weight loss in the obese or overweight cat. A more in-depth approach that centers on communication and commitment, alongside a program of feeding a predetermined amount of a specific diet plus exercise and enrichment of the cat's life, offers a chance for a healthy result. PATIENT GROUP: It has been reported in some developed countries that as much as 40-50% of the feline population may be overweight or obese, with middle-aged cats, male cats, mixed-breed cats and neutered cats being at greatest risk. AUDIENCE: This review of what is currently known about the health risks, predisposing factors and treatment of excessive weight gain in cats is aimed at all veterinary health professionals. EVIDENCE BASE: The information reported in the review is drawn from the current scientific literature as well as from the clinical experience of the authors.  相似文献